##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935185.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	277527
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.706215971779322	31.0	31.0	34.0	30.0	34.0
2	31.796574747682207	33.0	31.0	34.0	30.0	34.0
3	31.813441575053957	33.0	31.0	34.0	30.0	34.0
4	35.615968896719956	37.0	35.0	37.0	33.0	37.0
5	32.9395374143777	37.0	35.0	37.0	28.0	37.0
6	34.047184598255306	37.0	35.0	37.0	27.0	37.0
7	35.37469867796647	37.0	35.0	37.0	32.0	37.0
8	35.34051461659586	37.0	35.0	37.0	33.0	37.0
9	37.44201465082676	39.0	37.0	39.0	35.0	39.0
10	36.925362217009514	39.0	37.0	39.0	33.0	39.0
11	36.82155610084784	39.0	37.0	39.0	33.0	39.0
12	37.15198521224962	39.0	37.0	39.0	34.0	39.0
13	37.07385587708583	39.0	37.0	39.0	33.0	39.0
14	38.04606038331406	40.0	37.0	41.0	33.0	41.0
15	38.21012369967607	40.0	37.0	41.0	33.0	41.0
16	38.1787970179478	40.0	37.0	41.0	33.0	41.0
17	38.10784175954051	40.0	37.0	41.0	33.0	41.0
18	37.959758870308114	39.0	37.0	40.0	33.0	41.0
19	37.71217214901613	39.0	37.0	40.0	34.0	41.0
20	37.53702522637437	39.0	35.0	40.0	33.0	41.0
21	37.45300457252808	39.0	35.0	40.0	33.0	41.0
22	37.400083595469994	39.0	35.0	40.0	33.0	41.0
23	37.13378878451466	39.0	35.0	40.0	33.0	41.0
24	36.850854151127635	39.0	35.0	40.0	32.0	41.0
25	36.66731885546271	38.0	35.0	40.0	32.0	41.0
26	36.9245226590566	38.0	35.0	40.0	33.0	41.0
27	36.851733344863746	39.0	35.0	40.0	32.0	41.0
28	36.9230453253197	39.0	35.0	40.0	33.0	41.0
29	36.952318152828376	39.0	35.0	40.0	33.0	41.0
30	36.67428394354423	39.0	35.0	40.0	32.0	41.0
31	36.59950203043307	39.0	35.0	40.0	31.0	41.0
32	36.33875983237667	38.0	35.0	40.0	31.0	41.0
33	36.15632713213489	39.0	35.0	40.0	30.0	41.0
34	36.011894338208535	39.0	35.0	40.0	30.0	41.0
35	35.8212894601246	39.0	35.0	40.0	29.0	41.0
36	35.63076025035402	39.0	35.0	40.0	27.0	41.0
37	35.63251503457321	39.0	35.0	40.0	27.0	41.0
38	35.553034479528115	39.0	35.0	40.0	26.0	41.0
39	35.42436952080338	39.0	35.0	40.0	25.0	41.0
40	35.32942380381008	38.0	35.0	40.0	25.0	41.0
41	35.289510570142724	38.0	35.0	40.0	24.0	41.0
42	35.36897671217575	39.0	35.0	40.0	24.0	41.0
43	35.24696696177309	39.0	35.0	40.0	23.0	41.0
44	35.11902265365172	38.0	35.0	40.0	23.0	41.0
45	35.12371769233264	38.0	35.0	40.0	23.0	41.0
46	35.028357601242405	38.0	35.0	40.0	23.0	41.0
47	34.989092953118075	38.0	35.0	40.0	23.0	41.0
48	34.93458294147957	38.0	35.0	40.0	23.0	41.0
49	34.92575857484137	38.0	35.0	40.0	23.0	41.0
50	34.842573875694974	38.0	35.0	40.0	22.0	41.0
51	33.9541233825898	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	6.0
11	3.0
12	7.0
13	14.0
14	10.0
15	23.0
16	31.0
17	74.0
18	135.0
19	223.0
20	395.0
21	628.0
22	956.0
23	1408.0
24	2110.0
25	3127.0
26	4308.0
27	4867.0
28	4913.0
29	4949.0
30	5197.0
31	6363.0
32	7872.0
33	10415.0
34	16044.0
35	25669.0
36	29984.0
37	37850.0
38	58919.0
39	51011.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.37899735881554	23.97388362213406	23.166394621063898	18.480724397986503
2	35.241255805741424	25.837846407736905	23.981450453469392	14.93944733305228
3	26.901166373001544	25.27609926241411	34.89534351612636	12.927390848457987
4	23.667607115704055	27.427241313457788	33.89544080395781	15.00971076688034
5	18.098058927599837	34.73644005808444	32.183535295664925	14.981965718650796
6	20.784644376943504	34.17000868383977	32.67718095896976	12.368165980246967
7	63.94188673534467	9.965516868628999	21.20586465461018	4.886731741416151
8	61.80551802166996	9.703560374305924	21.187127738922698	7.303793865101414
9	55.922847146403775	13.267898258547817	23.603469212004597	7.205785383043812
10	22.061637246105782	38.80018881045808	28.36372677253024	10.774447170905894
11	19.651421303152485	26.89107726455444	35.912181517473975	17.5453199148191
12	19.530352001787215	23.58689424812721	39.938456438472656	16.944297311612925
13	19.344424146119117	25.42022938308705	41.28607306676467	13.949273404029158
14	14.286898211705529	30.037437798844795	37.92820158038677	17.7474624090629
15	12.92630987255294	28.980603689010437	41.861152248249724	16.2319341901869
16	13.707495126600294	30.263001437697955	37.36645443506398	18.663049000637777
17	13.6563289337614	29.760347641851066	35.671123890648474	20.91219953373906
18	12.490676582818969	29.760347641851066	40.64541468037344	17.103561094956525
19	14.214833151369055	29.735485196034983	38.00098729132661	18.048694361269355
20	14.051966115008629	32.067150223221525	38.399867400288976	15.481016261480866
21	15.22230269487293	30.548739401932064	38.539673617341734	15.689284285853269
22	12.32276499223499	30.165713606243717	35.61995769780958	21.89156370371171
23	12.618592064916207	28.87430772501414	37.68173907403604	20.82536113603361
24	15.24824611659406	28.284455206160118	36.01343292724672	20.4538657499991
25	12.773171619337939	31.44811135493123	33.98047757515485	21.79823945057598
26	11.60031276236186	33.366483261088106	36.33988765057094	18.693316325979094
27	15.776843334162082	29.157163086834792	37.365013133857246	17.700980445145877
28	12.81929325795328	30.090765943493786	36.923254313994676	20.166686484558262
29	15.451109261441228	29.811153509388276	35.07658714287258	19.66115008629791
30	16.529202564074847	31.88158269285511	34.46655640712435	17.122658335945694
31	17.44911305926991	32.60331427212487	30.481358570517465	19.466214098087754
32	17.248051540931154	32.85554198330253	31.57026163220155	18.32614484356477
33	18.52648571130016	30.736468884108575	31.079138246008498	19.65790715858277
34	16.65819902207713	29.55676384640053	31.31983554753231	22.465201583990027
35	16.60451055212646	30.20498906412709	32.01958728339945	21.170913100346993
36	19.17399027842336	32.20803741617932	31.759432415584786	16.858539889812523
37	20.560882364598758	28.299228543529097	33.65510382773568	17.484785264136463
38	20.839053497497538	31.26506610167659	29.598201256094004	18.297679144731863
39	22.972539608758787	28.65991417051314	29.287240520742124	19.080305699985946
40	18.486489602813418	28.99141344806091	32.31757630789077	20.204520641234907
41	16.87835778140505	29.071405665034394	31.528824222508078	22.521412331052474
42	21.239735232968325	27.62938380770159	32.84509254955374	18.285788409776345
43	24.408435935962988	25.30528561185038	32.073275753350124	18.213002698836508
44	20.648801738209254	27.38148000014413	29.802505702147897	22.167212559498715
45	20.325589942600182	25.656242455688993	30.03635682293975	23.981810778771074
46	20.865357244520354	27.70649342226162	32.449815693608194	18.97833363960984
47	18.362177373733008	26.500844962832442	36.1813445178307	18.95563314560385
48	18.461627156997338	25.947385299448346	34.35953979252469	21.23144775102963
49	21.81049051083318	23.581489368601975	35.43727276985663	19.17074735070822
50	19.530352001787215	22.853992584505292	33.9120157678352	23.703639645872293
51	18.420189747303866	22.22918851138808	30.52675955852944	28.823862182778615
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5656.0
1	6032.0
2	6408.0
3	9295.5
4	12183.0
5	7907.0
6	3631.0
7	3601.0
8	3571.0
9	3518.0
10	3465.0
11	3676.0
12	3887.0
13	3810.0
14	3733.0
15	3475.5
16	3218.0
17	2889.0
18	2560.0
19	2409.5
20	2259.0
21	2499.5
22	2740.0
23	2758.0
24	2776.0
25	3666.0
26	4697.0
27	4838.0
28	4844.0
29	4850.0
30	4938.0
31	5026.0
32	5163.0
33	5300.0
34	5493.5
35	5687.0
36	5991.0
37	6295.0
38	6978.5
39	7662.0
40	8426.5
41	9191.0
42	11202.0
43	13213.0
44	15501.5
45	17790.0
46	21946.0
47	26102.0
48	30841.0
49	35580.0
50	33606.0
51	31632.0
52	24203.5
53	16775.0
54	12708.5
55	8642.0
56	6757.0
57	4872.0
58	4339.5
59	3807.0
60	3545.5
61	3284.0
62	2968.0
63	2652.0
64	2118.0
65	1584.0
66	1199.5
67	815.0
68	684.5
69	554.0
70	437.0
71	320.0
72	250.0
73	180.0
74	146.5
75	90.5
76	68.0
77	45.0
78	22.0
79	15.0
80	8.0
81	13.0
82	18.0
83	10.0
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	277527.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.97955626080028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.08961110241903	26.23992771901135
2	12.585371412504426	9.559736432485586
3	5.368200339094313	6.116456003936388
4	3.005633713053899	4.566105388171544
5	2.0212889423757234	3.838382855314614
6	1.4420891114197747	3.286194272415289
7	1.1211668104922499	2.9806992569782684
8	0.9228428833748742	2.803933056721215
9	0.734487012798653	2.5105941742872218
>10	3.521949717857595	20.884523752930825
>50	0.11560446135476744	3.0719102986974023
>100	0.055809050309198076	3.4920458866168356
>500	0.0069761312886497595	1.8421620778413326
>1k	0.007972721472742582	6.088938020594519
>5k	9.965901840928227E-4	2.718390803997627
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7182	2.587856316682701	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4956	1.7857721951377703	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2078	0.7487559768959416	No Hit
GCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	2075	0.7476750009908946	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCC	1784	0.6428203382013281	No Hit
CCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	1762	0.6348931815643163	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGCT	1176	0.42374255477845396	TruSeq Adapter, Index 19 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1145	0.4125724704263009	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1111	0.40032141016910067	No Hit
TCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	964	0.3473535908217939	TruSeq Adapter, Index 13 (95% over 22bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	800	0.28826024134588707	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	698	0.25150706057428646	No Hit
ACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	687	0.24754348225578052	TruSeq Adapter, Index 19 (95% over 22bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	666	0.239976650920451	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCG	545	0.19637728941688556	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	507	0.1826849279529559	No Hit
ACCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTG	372	0.13404101222583747	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGC	358	0.12899645800228446	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	355	0.12791548209723738	No Hit
GCCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTG	307	0.11061986761648417	TruSeq Adapter, Index 13 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTG	294	0.10593563869461349	TruSeq Adapter, Index 19 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.603253016823588E-4	0.0	0.0	0.45184792830967796	0.0
2	3.603253016823588E-4	0.0	0.0	2.554706388927924	0.0
3	3.603253016823588E-4	0.0	0.0	3.3892197876242673	0.0
4	3.603253016823588E-4	0.0	0.0	4.475240246894897	0.0
5	3.603253016823588E-4	0.0	0.0	7.2587532023911185	0.0
6	3.603253016823588E-4	0.0	0.0	8.698252782612142	0.0
7	3.603253016823588E-4	0.0	0.0	10.231076615968897	0.0
8	3.603253016823588E-4	0.0	0.0	12.608502956469101	0.0
9	3.603253016823588E-4	0.0	0.0	13.434008222623385	0.0
10	3.603253016823588E-4	0.0	0.0	15.866204008979306	0.0
11	3.603253016823588E-4	0.0	0.0	19.762761821372337	0.0
12	3.603253016823588E-4	0.0	0.0	22.698692379480196	0.0
13	3.603253016823588E-4	0.0	0.0	23.83768065809813	0.0
14	3.603253016823588E-4	0.0	0.0	24.255297682747983	0.0
15	3.603253016823588E-4	0.0	0.0	25.112151250148635	0.0
16	3.603253016823588E-4	0.0	0.0	26.905850601923415	0.0
17	3.603253016823588E-4	0.0	0.0	29.073567616844485	0.0
18	3.603253016823588E-4	0.0	0.0	31.30866546318016	0.0
19	3.603253016823588E-4	0.0	0.0	32.682585838494994	0.0
20	3.603253016823588E-4	0.0	0.0	34.30260839485888	0.0
21	3.603253016823588E-4	0.0	0.0	35.94569177053044	0.0
22	3.603253016823588E-4	0.0	0.0	37.60751206188947	0.0
23	3.603253016823588E-4	0.0	0.0	39.096736533742664	0.0
24	3.603253016823588E-4	0.0	0.0	40.053039884407646	0.0
25	3.603253016823588E-4	0.0	0.0	40.86701474090809	0.0
26	3.603253016823588E-4	0.0	0.0	41.51740191044475	0.0
27	3.603253016823588E-4	0.0	0.0	42.05500726055483	0.0
28	3.603253016823588E-4	0.0	0.0	42.652066285442494	0.0
29	3.603253016823588E-4	0.0	0.0	43.211651478955204	0.0
30	3.603253016823588E-4	0.0	0.0	43.83069034724549	0.0
31	3.603253016823588E-4	0.0	0.0	44.347036504556314	3.603253016823588E-4
32	3.603253016823588E-4	0.0	0.0	44.77186003523981	3.603253016823588E-4
33	3.603253016823588E-4	0.0	0.0	45.170740144202185	3.603253016823588E-4
34	3.603253016823588E-4	0.0	0.0	45.564936024242684	3.603253016823588E-4
35	3.603253016823588E-4	0.0	0.0	45.94688084402598	3.603253016823588E-4
36	3.603253016823588E-4	0.0	0.0	46.380712507251545	3.603253016823588E-4
37	3.603253016823588E-4	0.0	0.0	46.78103391742065	3.603253016823588E-4
38	3.603253016823588E-4	0.0	0.0	47.13415271306936	3.603253016823588E-4
39	3.603253016823588E-4	0.0	0.0	47.47321882195246	3.603253016823588E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	25	3.8831335E-5	45.000004	6
CCCTCGT	25	3.8831335E-5	45.000004	14
ATATGAG	25	3.8831335E-5	45.000004	37
AATATGG	35	1.2074815E-7	45.000004	2
CGGGACG	25	3.8831335E-5	45.000004	6
CAAGCGG	35	1.2074815E-7	45.000004	2
TTAAACG	25	3.8831335E-5	45.000004	37
TTACGCG	35	1.2074815E-7	45.000004	1
ACGTAAG	25	3.8831335E-5	45.000004	1
AGTCGTG	25	3.8831335E-5	45.000004	1
ATCGAGG	25	3.8831335E-5	45.000004	2
TCACGAC	20	7.0240296E-4	45.0	25
TATCAGG	20	7.0240296E-4	45.0	2
ATGGGCG	20	7.0240296E-4	45.0	5
CGTGGGT	20	7.0240296E-4	45.0	4
CTCACGA	20	7.0240296E-4	45.0	24
CAAACGG	20	7.0240296E-4	45.0	2
TTGTGCG	20	7.0240296E-4	45.0	1
GATCGAC	20	7.0240296E-4	45.0	10
CGCGGGT	20	7.0240296E-4	45.0	4
>>END_MODULE