FastQCFastQC Report
Sat 14 Jan 2017
SRR2935179.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935179.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844138
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310903.6830470847183756No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCC52740.6247793607206404No Hit
CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC34230.40550241785110963No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32380.38358656996841745No Hit
GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC27940.3309885350499563No Hit
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT24450.28964458417936406No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17570.208141322864271No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCG16920.20044116009467647No Hit
TCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC16130.19108250072855387No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14970.17734067178589283No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14930.17686681561545622No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13770.1631249866727952No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGC10950.1297181266570158No Hit
ACTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC10640.12604574133613225No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTC10240.12130717963176638No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9790.11597629771435476No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGT9640.11419933707521755No Hit
CGCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG9620.11396240898999928No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9350.11076387983955231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAG351.2114106E-745.0000041
ATGACCG207.0322194E-445.0000041
CGGTCTA1200.045.00000431
TCGAACG207.0322194E-445.0000041
ACGACAG351.2114106E-745.0000041
CGAACCA253.8899227E-545.015
CGCATCG253.8899227E-545.021
TTATGCG453.8380676E-1045.01
CGTTATT7650.044.1176451
CGACGGT1300.043.26923428
CGTTTTT90700.042.816981
CTCACGA1400.041.78571724
AACGAGC1100.040.90909215
CACAACG1100.040.90909212
TCACGAC1450.040.3448325
CACGACG1450.040.3448326
ACGGTCT1350.040.00000430
CGGGTAT451.9272193E-840.06
TATTGCG451.9272193E-840.01
CGTACGG451.9272193E-840.02
Produced by FastQC (version 0.11.5)