##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935179.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	844138
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72101481037461	31.0	31.0	34.0	30.0	34.0
2	31.828199891486936	33.0	31.0	34.0	30.0	34.0
3	31.79303384043841	33.0	31.0	34.0	30.0	34.0
4	35.58402299150139	37.0	35.0	37.0	33.0	37.0
5	33.173799781552304	37.0	35.0	37.0	28.0	37.0
6	34.1521966787421	37.0	35.0	37.0	28.0	37.0
7	35.44127263551694	37.0	35.0	37.0	32.0	37.0
8	35.41706213912891	37.0	35.0	37.0	33.0	37.0
9	37.472259275142214	39.0	37.0	39.0	35.0	39.0
10	36.91846830731468	39.0	37.0	39.0	32.0	39.0
11	36.846289350793356	39.0	37.0	39.0	33.0	39.0
12	37.07918965856294	39.0	37.0	39.0	33.0	39.0
13	37.022698895204336	39.0	37.0	39.0	33.0	39.0
14	37.88882031137089	40.0	37.0	41.0	33.0	41.0
15	38.05692552639497	40.0	37.0	41.0	33.0	41.0
16	38.07783087599421	40.0	37.0	41.0	33.0	41.0
17	38.03574060165518	40.0	37.0	41.0	33.0	41.0
18	37.88100997704167	39.0	37.0	40.0	33.0	41.0
19	37.6238150634138	39.0	37.0	40.0	33.0	41.0
20	37.38589661879929	39.0	35.0	40.0	33.0	41.0
21	37.28248106352279	39.0	35.0	40.0	33.0	41.0
22	37.279966071898194	39.0	35.0	40.0	33.0	41.0
23	37.02547213844182	39.0	35.0	40.0	32.0	41.0
24	36.726932089302935	38.0	35.0	40.0	32.0	41.0
25	36.54062724341281	38.0	35.0	40.0	31.0	41.0
26	36.822189025964946	38.0	35.0	40.0	32.0	41.0
27	36.768864806465295	39.0	35.0	40.0	32.0	41.0
28	36.81199993366014	39.0	35.0	40.0	32.0	41.0
29	36.85671655582381	39.0	35.0	40.0	32.0	41.0
30	36.569005304819825	39.0	35.0	40.0	31.0	41.0
31	36.366505239664605	39.0	35.0	40.0	31.0	41.0
32	36.11079823441191	39.0	35.0	40.0	30.0	41.0
33	35.86735581149054	39.0	35.0	40.0	29.0	41.0
34	35.668666734586054	39.0	35.0	40.0	26.0	41.0
35	35.46196474983948	39.0	35.0	40.0	25.0	41.0
36	35.28560614496682	39.0	35.0	40.0	24.0	41.0
37	35.2449101924093	39.0	35.0	40.0	23.0	41.0
38	35.18326268927592	39.0	35.0	40.0	23.0	41.0
39	35.04075755385968	39.0	35.0	40.0	23.0	41.0
40	34.90985715605742	38.0	35.0	40.0	22.0	41.0
41	34.76477068915272	38.0	35.0	40.0	21.0	41.0
42	34.82844866597642	38.0	35.0	40.0	21.0	41.0
43	34.77655075354978	38.0	35.0	40.0	21.0	41.0
44	34.727447407888285	38.0	35.0	40.0	20.0	41.0
45	34.71646223721714	38.0	35.0	40.0	20.0	41.0
46	34.613779974364384	38.0	35.0	40.0	20.0	41.0
47	34.510666502396525	38.0	34.0	40.0	20.0	41.0
48	34.45467328801689	38.0	34.0	40.0	20.0	41.0
49	34.441889833178934	38.0	34.0	40.0	20.0	41.0
50	34.352832119866655	38.0	34.0	40.0	19.0	41.0
51	33.4457884848212	37.0	33.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	15.0
10	31.0
11	22.0
12	23.0
13	42.0
14	74.0
15	105.0
16	161.0
17	289.0
18	502.0
19	879.0
20	1454.0
21	2287.0
22	3613.0
23	5526.0
24	8193.0
25	12141.0
26	15848.0
27	17455.0
28	16746.0
29	16120.0
30	17570.0
31	20019.0
32	24814.0
33	33371.0
34	48904.0
35	65111.0
36	84243.0
37	109131.0
38	174349.0
39	165036.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.48402512385415	21.924614221845243	23.086391087713146	22.504969566587455
2	40.20408985260704	20.702539158289284	24.75507559190559	14.33829539719809
3	26.95767753613746	21.502052981858416	38.50650012201796	13.033769359986163
4	24.082673686056072	23.586783203694182	37.254809047809715	15.075734062440027
5	18.300799158431442	31.164928009401304	34.82262378900132	15.711649043165929
6	20.590946030151468	31.07264452020878	35.63978875491922	12.696620694720531
7	66.10648969718221	6.5490476675614655	22.596779199609543	4.7476834356467785
8	64.98262132494924	6.022001141993371	22.90561495869159	6.089762574365802
9	58.93029338804793	9.30049352120151	25.28378061407021	6.485432476680353
10	23.314078977607927	35.11309762147895	29.863718965382436	11.709104435530683
11	20.868507281984698	22.236530046035128	38.67092821315946	18.224034458820714
12	21.054614292923667	20.062714864157282	41.42308485105516	17.45958599186389
13	21.220463952576473	20.24017399998578	43.402026682840955	15.137335364596785
14	15.504100040514704	23.41193027680308	42.81823588086308	18.265733801819135
15	13.18007245260846	24.04275130369679	44.726810071339045	18.050366172355705
16	15.827388412795065	25.321689107705136	40.117966493630185	18.73295598586961
17	15.800141682994962	24.53804946584563	39.57587503465073	20.085933816508675
18	15.361706261298508	23.673380418841468	43.23499238276206	17.729920937097962
19	15.25864254422855	25.49618664246841	41.29123437163118	17.953936441671857
20	14.9380788449282	27.899348210837566	40.80884879012673	16.353724154107503
21	15.028940765609414	26.13672172085607	42.5892448864996	16.245092627034914
22	13.791228448429049	23.76898090122705	40.81465352821458	21.62513712212932
23	12.930824106958816	24.3576287289519	41.873129748927305	20.838417415161974
24	14.746759416114427	24.38155846555895	39.931148698435564	20.94053341989106
25	13.95624885978359	26.453968426963364	37.712198716323634	21.877583996929413
26	12.750047977937257	26.420206174819754	39.27296247769914	21.55678336954384
27	13.913009484231251	24.679140140593127	41.60303173177846	19.80481864339717
28	11.63873679422085	24.046542153060283	41.51098517067114	22.80373588204772
29	15.130819842253281	23.509781575998236	39.003575244805944	22.35582333694254
30	18.1772411619901	23.983400818349608	38.953228026697055	18.886129992963234
31	15.40861802217173	26.515451265077512	37.93183105132099	20.144099661429767
32	17.310084370091147	25.777894135792963	34.6837839310634	22.228237563052485
33	18.618401256666566	24.65627658036956	35.424421125455794	21.300901037508087
34	15.990750327553076	22.814279181839936	37.53118565921686	23.663784831390128
35	16.446126107342636	23.88128481362052	34.07997270588458	25.592616373152254
36	16.920693062034882	25.82445050453836	36.748849121826055	20.50600731160071
37	16.874018229246875	25.335194008562585	38.962942078191006	18.827845683999538
38	20.487526920953684	25.08985497631904	33.9286941234727	20.49392397925458
39	22.589908285138215	23.215635358199727	33.79838367660264	20.396072680059422
40	20.687375760835312	21.82901373945966	35.798056715845036	21.685553783859984
41	16.003781372240084	24.881950581539986	33.888653277070816	25.225614769149118
42	16.9610893005646	25.272763458107562	35.18536068747053	22.58078655385731
43	18.80486365973336	23.566051996237583	36.48408198659461	21.145002357434446
44	20.061767151816408	22.058952446164014	34.019200652026086	23.860079749993485
45	21.253752348549646	19.620962449267772	34.48808133267309	24.637203869509488
46	20.991472958212995	21.424695962034644	36.00750114317801	21.57632993657435
47	16.006387581177485	23.926775005982435	39.22877538980593	20.838062023034148
48	17.238176696227395	23.448772594054525	37.105544354122195	22.207506355595886
49	20.879405973904742	20.880709078373442	37.334772276570895	20.905112671150924
50	19.244720649941126	19.69938564547503	36.20557302242051	24.850320682163343
51	17.806567172666078	19.941644612610734	33.41361246620813	28.838175748515056
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5448.0
1	8169.5
2	10891.0
3	29219.5
4	47548.0
5	29424.0
6	11300.0
7	10958.5
8	10617.0
9	10722.5
10	10828.0
11	10813.5
12	10799.0
13	10596.5
14	10394.0
15	9939.5
16	9485.0
17	9132.0
18	8779.0
19	8206.5
20	7634.0
21	7706.5
22	7779.0
23	8161.5
24	8544.0
25	9196.5
26	10186.0
27	10523.0
28	11069.5
29	11616.0
30	12649.5
31	13683.0
32	14424.5
33	15166.0
34	16880.5
35	18595.0
36	19854.5
37	21114.0
38	21686.0
39	22258.0
40	25235.5
41	28213.0
42	34047.0
43	39881.0
44	45457.0
45	51033.0
46	62572.0
47	74111.0
48	87785.0
49	101459.0
50	98742.5
51	96026.0
52	77155.5
53	58285.0
54	45711.5
55	33138.0
56	27598.0
57	22058.0
58	19727.5
59	17397.0
60	14729.5
61	12062.0
62	10685.0
63	9308.0
64	7969.0
65	6630.0
66	5310.5
67	3991.0
68	3348.0
69	2705.0
70	2424.0
71	2143.0
72	1752.0
73	1361.0
74	1102.0
75	601.0
76	359.0
77	259.0
78	159.0
79	118.0
80	77.0
81	50.0
82	23.0
83	21.5
84	20.0
85	11.5
86	3.0
87	2.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	844138.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.36565962092511
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.55878796882288	28.885176780354556
2	11.090707580354223	8.953674542875852
3	4.941084607090055	5.98350418223969
4	2.847291066433727	4.597311281174588
5	1.9172390080282167	3.869530860501355
6	1.4301519157174596	3.463741526163897
7	1.0986770997247268	3.1044178088555463
8	0.8998374045601746	2.9058024309322157
9	0.7156252752651473	2.5998017649735394
>10	3.3442044167054927	20.97398703051229
>50	0.09782159395509311	2.7289430040747478
>100	0.05162795274047451	3.747415455908508
>500	0.002415342818227725	0.767666264156272
>1k	0.003924932079620054	2.9873052019365836
>5k	3.0191785227846565E-4	0.6427483532561008
>10k+	3.0191785227846565E-4	3.788973512084219
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31090	3.6830470847183756	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCC	5274	0.6247793607206404	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	3423	0.40550241785110963	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3238	0.38358656996841745	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	2794	0.3309885350499563	No Hit
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT	2445	0.28964458417936406	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1757	0.208141322864271	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCG	1692	0.20044116009467647	No Hit
TCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	1613	0.19108250072855387	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1497	0.17734067178589283	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1493	0.17686681561545622	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1377	0.1631249866727952	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGC	1095	0.1297181266570158	No Hit
ACTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	1064	0.12604574133613225	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTC	1024	0.12130717963176638	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	979	0.11597629771435476	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGT	964	0.11419933707521755	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG	962	0.11396240898999928	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	935	0.11076387983955231	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.30540030184638056	0.0
2	0.0	0.0	0.0	1.4248855045028184	0.0
3	0.0	0.0	0.0	1.9827326811492907	0.0
4	0.0	0.0	0.0	2.7459965076800237	0.0
5	0.0	0.0	0.0	4.815444868019211	0.0
6	0.0	0.0	0.0	6.1968540688844715	0.0
7	0.0	0.0	0.0	7.529693012279982	0.0
8	0.0	0.0	0.0	9.59084888963653	0.0
9	0.0	0.0	0.0	10.434312873013655	0.0
10	0.0	0.0	0.0	12.806673790304428	0.0
11	0.0	0.0	0.0	16.585202893365775	0.0
12	0.0	0.0	0.0	19.34659972658499	0.0
13	0.0	0.0	0.0	20.399745065380305	0.0
14	0.0	0.0	0.0	20.862110223683807	0.0
15	1.1846404260914685E-4	0.0	0.0	21.662097903423373	0.0
16	1.1846404260914685E-4	0.0	0.0	23.26752260886253	0.0
17	1.1846404260914685E-4	0.0	0.0	25.215426861484733	0.0
18	2.369280852182937E-4	0.0	0.0	27.194842549440967	0.0
19	2.369280852182937E-4	0.0	0.0	28.446533623649213	0.0
20	2.369280852182937E-4	0.0	0.0	29.933020430308787	0.0
21	2.369280852182937E-4	0.0	0.0	31.594360163859463	0.0
22	2.369280852182937E-4	0.0	0.0	33.26268927592408	0.0
23	2.369280852182937E-4	0.0	0.0	34.61543017847793	0.0
24	2.369280852182937E-4	0.0	0.0	35.4931302701691	0.0
25	2.369280852182937E-4	0.0	0.0	36.2274888703032	0.0
26	2.369280852182937E-4	0.0	0.0	36.84800352548991	0.0
27	2.369280852182937E-4	0.0	0.0	37.45347324726526	0.0
28	2.369280852182937E-4	0.0	0.0	38.01072810369868	0.0
29	2.369280852182937E-4	0.0	0.0	38.551871850337264	0.0
30	2.369280852182937E-4	0.0	0.0	39.142296638701254	0.0
31	2.369280852182937E-4	0.0	0.0	39.60501718913258	0.0
32	2.369280852182937E-4	0.0	0.0	40.0442818591273	0.0
33	2.369280852182937E-4	0.0	0.0	40.42964538973485	0.0
34	2.369280852182937E-4	0.0	0.0	40.84687574780427	0.0
35	3.5539212782744053E-4	0.0	0.0	41.22856689309094	0.0
36	3.5539212782744053E-4	0.0	0.0	41.58869758262275	0.0
37	3.5539212782744053E-4	0.0	0.0	41.93449412299885	0.0
38	3.5539212782744053E-4	0.0	0.0	42.32649163999251	0.0
39	3.5539212782744053E-4	0.0	0.0	42.917508748569546	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAG	35	1.2114106E-7	45.000004	1
ATGACCG	20	7.0322194E-4	45.000004	1
CGGTCTA	120	0.0	45.000004	31
TCGAACG	20	7.0322194E-4	45.000004	1
ACGACAG	35	1.2114106E-7	45.000004	1
CGAACCA	25	3.8899227E-5	45.0	15
CGCATCG	25	3.8899227E-5	45.0	21
TTATGCG	45	3.8380676E-10	45.0	1
CGTTATT	765	0.0	44.117645	1
CGACGGT	130	0.0	43.269234	28
CGTTTTT	9070	0.0	42.81698	1
CTCACGA	140	0.0	41.785717	24
AACGAGC	110	0.0	40.909092	15
CACAACG	110	0.0	40.909092	12
TCACGAC	145	0.0	40.34483	25
CACGACG	145	0.0	40.34483	26
ACGGTCT	135	0.0	40.000004	30
CGGGTAT	45	1.9272193E-8	40.0	6
TATTGCG	45	1.9272193E-8	40.0	1
CGTACGG	45	1.9272193E-8	40.0	2
>>END_MODULE