SRR2935178.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935178.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	297869
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7426	2.493042243402301	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7305	2.4524203592854574	No Hit
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	3726	1.250885456358332	No Hit
GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	2762	0.9272532556257953	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2486	0.8345950736733263	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCC	2358	0.7916231632026159	No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT	2328	0.7815516216860432	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	1504	0.5049199480308458	No Hit
ACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	833	0.27965313611016923	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCG	781	0.26219579748144317	No Hit
CGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG	668	0.22425965776901924	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	655	0.21989532311183774	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGC	518	0.17390195018615565	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	500	0.16785902527621202	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	450	0.15107312274859083	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	439	0.14738022419251415	No Hit
GCCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG	436	0.1463730700408569	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	435	0.14603735199030446	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTC	429	0.14402304368698993	No Hit
ACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG	418	0.14033014513091324	No Hit
AGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG	412	0.1383158368275987	No Hit
AGCCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT	381	0.12790857726047358	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	379	0.1272371411593687	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGT	375	0.12589426895715902	No Hit
GGCCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT	331	0.11112267473285237	No Hit
GGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG	313	0.10507974982290874	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGA	25	3.883824E-5	45.000004	24
GATAAAT	25	3.883824E-5	45.000004	9
GACCGTG	25	3.883824E-5	45.000004	27
CCGTGAT	25	3.883824E-5	45.000004	29
CACGACG	25	3.883824E-5	45.000004	26
TGTGCGG	50	2.1827873E-11	45.000004	2
CTACGAA	25	3.883824E-5	45.000004	11
GTGTACG	25	3.883824E-5	45.000004	1
TTAGTGG	25	3.883824E-5	45.000004	2
CGGTCTA	25	3.883824E-5	45.000004	31
GCTACGA	25	3.883824E-5	45.000004	10
CAACTCA	25	3.883824E-5	45.000004	20
AGGTGTA	25	3.883824E-5	45.000004	15
CCGCAGG	25	3.883824E-5	45.000004	2
GTCGGAG	25	3.883824E-5	45.000004	1
GCATATC	55	1.8189894E-12	45.000004	9
CTTAAGG	40	6.7866495E-9	45.0	2
ATGGGAC	105	0.0	45.0	5
CGGAAGG	35	1.2078817E-7	45.0	2
GCCTTGG	20	7.0248626E-4	45.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content