##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935178.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	297869
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.614515105633686	31.0	31.0	34.0	30.0	34.0
2	31.71613024517489	31.0	31.0	34.0	30.0	34.0
3	31.730072615814336	31.0	31.0	34.0	30.0	34.0
4	35.54671013096361	37.0	35.0	37.0	33.0	37.0
5	33.101594996458175	37.0	35.0	37.0	28.0	37.0
6	34.088176345977594	37.0	35.0	37.0	28.0	37.0
7	35.31347001534232	37.0	35.0	37.0	32.0	37.0
8	35.28830794745341	37.0	35.0	37.0	33.0	37.0
9	37.35350439287069	39.0	37.0	39.0	34.0	39.0
10	36.89922079840467	39.0	37.0	39.0	33.0	39.0
11	36.76787446830654	39.0	37.0	39.0	32.0	39.0
12	37.08942185994514	39.0	37.0	39.0	33.0	39.0
13	37.03234979135123	39.0	37.0	39.0	33.0	39.0
14	37.99358442805394	40.0	37.0	41.0	33.0	41.0
15	38.12790522007997	40.0	37.0	41.0	33.0	41.0
16	38.09035515612568	40.0	37.0	41.0	33.0	41.0
17	38.040148521665564	39.0	37.0	41.0	33.0	41.0
18	37.93770751572	39.0	37.0	40.0	33.0	41.0
19	37.691287109434015	39.0	36.0	40.0	33.0	41.0
20	37.52619775807486	39.0	35.0	40.0	33.0	41.0
21	37.43595338890586	39.0	35.0	40.0	33.0	41.0
22	37.34730032329648	39.0	35.0	40.0	33.0	41.0
23	37.09220831976473	39.0	35.0	40.0	32.0	41.0
24	36.81593586442362	39.0	35.0	40.0	32.0	41.0
25	36.59791049085336	38.0	35.0	40.0	31.0	41.0
26	36.81144395690723	38.0	35.0	40.0	32.0	41.0
27	36.712454132521344	38.0	35.0	40.0	32.0	41.0
28	36.73665604678567	38.0	35.0	40.0	32.0	41.0
29	36.854107006771436	39.0	35.0	40.0	33.0	41.0
30	36.64404150817977	38.0	35.0	40.0	32.0	41.0
31	36.432277276252314	38.0	35.0	40.0	31.0	41.0
32	36.215000553934786	38.0	35.0	40.0	31.0	41.0
33	36.01196163414118	38.0	35.0	40.0	30.0	41.0
34	35.91006784861802	38.0	35.0	40.0	30.0	41.0
35	35.7437866981794	38.0	35.0	40.0	30.0	41.0
36	35.42963517519446	38.0	35.0	40.0	27.0	41.0
37	35.382550718604485	38.0	35.0	40.0	27.0	41.0
38	35.36265270974825	38.0	35.0	40.0	27.0	41.0
39	35.32064431008262	38.0	35.0	40.0	27.0	41.0
40	35.30147145221557	38.0	35.0	40.0	26.0	41.0
41	35.106694553646065	38.0	35.0	40.0	25.0	41.0
42	35.01114583927834	38.0	35.0	40.0	24.0	41.0
43	34.895927404328745	38.0	35.0	40.0	24.0	41.0
44	34.897730210260214	38.0	35.0	40.0	24.0	41.0
45	34.99766675954866	38.0	35.0	40.0	24.0	41.0
46	34.91362981713438	38.0	35.0	40.0	23.0	41.0
47	34.77000627792754	38.0	34.0	40.0	23.0	41.0
48	34.71089640076678	38.0	34.0	40.0	23.0	41.0
49	34.71255820511701	38.0	34.0	40.0	23.0	41.0
50	34.66433902151617	38.0	34.0	40.0	23.0	41.0
51	33.81078930670866	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	8.0
10	10.0
11	13.0
12	9.0
13	16.0
14	12.0
15	26.0
16	31.0
17	64.0
18	134.0
19	257.0
20	460.0
21	694.0
22	1025.0
23	1547.0
24	2264.0
25	3373.0
26	4371.0
27	4650.0
28	4790.0
29	4872.0
30	5482.0
31	6837.0
32	9066.0
33	12252.0
34	18520.0
35	30901.0
36	36365.0
37	43932.0
38	63124.0
39	42748.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.36611396284944	24.18244261739221	23.157159691005106	18.294283728753243
2	35.958424676619586	25.396399088189774	24.047483961070135	14.597692274120503
3	26.0644780087891	24.88476477914788	36.15884835279938	12.891908859263637
4	24.716570035821118	25.658259167620667	34.432921854909374	15.192248941648845
5	17.918615230185083	33.17028626678171	32.46024258986333	16.450855913169885
6	19.870815694147428	33.39286733429797	32.656973367487055	14.07934360406756
7	61.32930919296737	10.960187196384988	21.585663496369207	6.124840114278425
8	59.39825896618983	9.592807576484965	21.56484897723496	9.444084480090241
9	53.84212522954722	12.859008490309499	24.774984976617237	8.523881303526046
10	23.580835870802268	32.89734749168258	30.424112613262878	13.097704024252272
11	16.3420832647909	27.34792811605102	35.98763214701765	20.322356472140438
12	16.64960099909692	23.89473224806878	41.95099187898036	17.504674873853943
13	18.576958327318387	26.834615216756358	40.945180599525294	13.643245856399961
14	13.63485290513615	29.863799186890883	37.674951069094135	18.826396838878836
15	10.774535114429499	30.24953922697562	40.6826490839933	18.293276574601585
16	11.059559739348506	30.96059005804565	36.604681923933	21.37516827867284
17	10.664755311898855	31.50344614578892	34.431914700757716	23.39988384155451
18	10.493203388066567	29.620403600240376	41.33024920350892	18.55614380818413
19	10.949444218767312	30.64467937247582	38.16946375755785	20.236412651199018
20	12.118750188841403	33.20587238014026	38.032826510983014	16.64255092003532
21	13.070175144106974	32.2410187028526	38.629733204865225	16.059072948175203
22	10.551954046913242	32.10606004653052	33.58859095777002	23.75339494878621
23	10.565382768935336	30.62890062409986	34.64710997116182	24.158606635802986
24	14.787037254632072	29.676804232733183	33.55099053610815	21.985167976526593
25	10.449224323444199	33.68527775632912	31.172092429893677	24.693405490333
26	10.885993507212904	35.5172911581937	33.35325260433278	20.243462730260617
27	16.17523139366634	32.526379045822154	33.39152446209575	17.906865098415746
28	10.203814428490377	33.35190973213057	33.61511268376367	22.829163155615387
29	13.710389466510446	31.152285064911084	34.985849484169215	20.151475984409252
30	22.354793550184812	32.02313768804407	28.837509106352123	16.78455965541899
31	18.51216474356177	37.63970067378612	25.785832026830587	18.062302555821518
32	19.302109316511622	34.355370985231765	28.287602939547252	18.054916758709368
33	22.04828297003045	29.27159254571641	29.59086041179176	19.089264072461383
34	19.12887880242657	30.42948410207172	30.066237171373995	20.37539992412772
35	21.610506632110088	30.43250556452669	27.430179038436357	20.526808764926862
36	25.192618231504454	32.03186635735843	28.415847234858276	14.359668176278834
37	20.885691360967403	32.556593670371875	32.33300544870396	14.224709519956761
38	20.425757631710585	31.768663405725334	30.478163219401814	17.327415743162263
39	25.37659172320718	26.304180696883527	32.315548110075234	16.003679469834054
40	29.613353521178777	25.740845808056562	28.693821780715684	15.951978890048983
41	25.556200880252728	29.084933309609255	25.844918403727814	19.5139474064102
42	22.58408897871212	27.345913807747703	32.016759045083575	18.053238168456602
43	22.85467772745737	26.684213530108874	33.46101809856011	17.00009064387365
44	20.40057877791915	26.33976681024209	30.55504265297832	22.704611758860437
45	24.548375292494352	24.194528467212095	28.3359463388268	22.92114990146675
46	27.609116759380804	25.067731116698948	31.379566185135076	15.943585938785171
47	20.11756846130346	27.24083405792479	36.265942410925604	16.37565506984614
48	19.696242307860167	24.50238191956867	35.189294622803985	20.61208114976718
49	22.720390507236402	21.877402482299267	37.63399346692673	17.768213543537595
50	22.55622438051627	20.293820437843483	33.547969073653185	23.60198610798707
51	20.354249686942918	19.85067261111428	29.700640214322405	30.094437487620397
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7955.0
1	6908.5
2	5862.0
3	9093.0
4	12324.0
5	7747.5
6	3171.0
7	3223.0
8	3275.0
9	3737.5
10	4200.0
11	4681.5
12	5163.0
13	5074.0
14	4985.0
15	4707.5
16	4430.0
17	3874.0
18	3318.0
19	3048.5
20	2779.0
21	2578.0
22	2377.0
23	2404.5
24	2432.0
25	2412.0
26	2279.5
27	2167.0
28	2376.0
29	2585.0
30	2774.0
31	2963.0
32	3125.0
33	3287.0
34	3692.5
35	4098.0
36	4433.0
37	4768.0
38	5217.5
39	5667.0
40	6951.0
41	8235.0
42	11106.5
43	13978.0
44	16752.0
45	19526.0
46	25289.0
47	31052.0
48	39147.0
49	47242.0
50	45768.0
51	44294.0
52	32705.0
53	21116.0
54	15189.0
55	9262.0
56	6835.5
57	4409.0
58	3498.0
59	2587.0
60	2260.0
61	1933.0
62	1713.0
63	1493.0
64	1216.0
65	939.0
66	776.0
67	613.0
68	535.0
69	457.0
70	354.0
71	251.0
72	188.5
73	126.0
74	116.5
75	62.5
76	18.0
77	18.0
78	18.0
79	13.5
80	9.0
81	6.5
82	4.0
83	2.5
84	1.0
85	0.5
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	297869.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.84010328320577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.81884777442319	23.976557642921193
2	12.318576004107	8.583609205702162
3	5.423399223003782	5.668553672265291
4	3.057653450767684	4.261158481159865
5	2.0110781333446215	3.5033084938161636
6	1.470096655634221	3.073099159115498
7	1.0738758879904302	2.6189762795651634
8	0.8752684021832572	2.4395553226072937
9	0.6327481657799361	1.9840510303228778
>10	4.092902541660553	23.45394483738364
>50	0.1327373903850088	3.239990778286414
>100	0.07984203932932862	5.600962809254942
>500	0.004990127458083039	1.2013488022085812
>1k	0.005988152949699646	5.272721631459023
>5k	0.0019960509832332154	5.122161853931869
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7426	2.493042243402301	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7305	2.4524203592854574	No Hit
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	3726	1.250885456358332	No Hit
GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	2762	0.9272532556257953	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2486	0.8345950736733263	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCC	2358	0.7916231632026159	No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT	2328	0.7815516216860432	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	1504	0.5049199480308458	No Hit
ACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	833	0.27965313611016923	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCG	781	0.26219579748144317	No Hit
CGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG	668	0.22425965776901924	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	655	0.21989532311183774	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGC	518	0.17390195018615565	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	500	0.16785902527621202	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	450	0.15107312274859083	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	439	0.14738022419251415	No Hit
GCCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG	436	0.1463730700408569	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	435	0.14603735199030446	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTC	429	0.14402304368698993	No Hit
ACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG	418	0.14033014513091324	No Hit
AGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG	412	0.1383158368275987	No Hit
AGCCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT	381	0.12790857726047358	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	379	0.1272371411593687	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGT	375	0.12589426895715902	No Hit
GGCCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT	331	0.11112267473285237	No Hit
GGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG	313	0.10507974982290874	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8627953899197298	0.0
2	0.0	0.0	0.0	4.1511536950807235	0.0
3	0.0	0.0	0.0	5.4621326824879395	0.0
4	0.0	0.0	0.0	7.0077785872313	0.0
5	0.0	0.0	0.0	10.75271344114359	0.0
6	0.0	0.0	0.0	12.635755986692136	0.0
7	0.0	0.0	0.0	14.670543091090378	0.0
8	0.0	0.0	0.0	17.539925269161948	0.0
9	0.0	0.0	0.0	18.61623733923302	0.0
10	0.0	0.0	0.0	21.792801533560056	0.0
11	0.0	0.0	0.0	27.307641949984724	0.0
12	0.0	0.0	0.0	31.087155763103915	0.0
13	0.0	0.0	0.0	32.4703141313799	0.0
14	0.0	0.0	0.0	32.99739147074721	0.0
15	0.0	0.0	0.0	34.03744599135862	0.0
16	0.0	0.0	0.0	36.31126434775018	0.0
17	0.0	0.0	0.0	39.05139507635907	0.0
18	0.0	0.0	0.0	41.727739375362994	0.0
19	0.0	0.0	0.0	43.48656624220715	0.0
20	0.0	0.0	0.0	44.93921824694748	0.0
21	0.0	0.0	0.0	46.736652689605165	0.0
22	0.0	0.0	0.0	48.50655825211754	0.0
23	0.0	0.0	0.0	49.97935333989103	0.0
24	0.0	0.0	0.0	50.99926477746929	0.0
25	0.0	0.0	0.0	51.793237967025775	0.0
26	0.0	0.0	0.0	52.499588745388074	0.0
27	0.0	0.0	0.0	53.07433804793382	0.0
28	0.0	0.0	0.0	53.62323706058704	0.0
29	0.0	0.0	0.0	54.16105737757202	0.0
30	0.0	0.0	0.0	54.70659920971971	0.0
31	0.0	0.0	0.0	55.17794735269531	0.0
32	0.0	0.0	0.0	55.54253715559525	0.0
33	0.0	0.0	0.0	55.87691233394546	0.0
34	0.0	0.0	0.0	56.16965847402717	0.0
35	0.0	0.0	0.0	56.487247749849764	0.0
36	0.0	0.0	0.0	56.81423713108783	0.0
37	0.0	0.0	0.0	57.12477632784882	0.0
38	0.0	0.0	0.0	57.39805082099849	0.0
39	0.0	0.0	0.0	57.66360379898546	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGA	25	3.883824E-5	45.000004	24
GATAAAT	25	3.883824E-5	45.000004	9
GACCGTG	25	3.883824E-5	45.000004	27
CCGTGAT	25	3.883824E-5	45.000004	29
CACGACG	25	3.883824E-5	45.000004	26
TGTGCGG	50	2.1827873E-11	45.000004	2
CTACGAA	25	3.883824E-5	45.000004	11
GTGTACG	25	3.883824E-5	45.000004	1
TTAGTGG	25	3.883824E-5	45.000004	2
CGGTCTA	25	3.883824E-5	45.000004	31
GCTACGA	25	3.883824E-5	45.000004	10
CAACTCA	25	3.883824E-5	45.000004	20
AGGTGTA	25	3.883824E-5	45.000004	15
CCGCAGG	25	3.883824E-5	45.000004	2
GTCGGAG	25	3.883824E-5	45.000004	1
GCATATC	55	1.8189894E-12	45.000004	9
CTTAAGG	40	6.7866495E-9	45.0	2
ATGGGAC	105	0.0	45.0	5
CGGAAGG	35	1.2078817E-7	45.0	2
GCCTTGG	20	7.0248626E-4	45.0	2
>>END_MODULE