SRR2935177.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935177.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	231638
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5764	2.4883654668059645	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2146	0.9264455745603053	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCC	1959	0.8457161605608752	No Hit
GCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	1489	0.6428133553216657	RNA PCR Primer, Index 40 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	1468	0.6337474853003393	RNA PCR Primer, Index 46 (96% over 26bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCG	945	0.4079641509596871	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTC	930	0.40148852951588254	RNA PCR Primer, Index 46 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGCT	844	0.36436163323806975	RNA PCR Primer, Index 46 (96% over 27bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGC	776	0.33500548269282243	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	730	0.31514691026515507	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCG	628	0.27111268444728415	No Hit
TCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	569	0.24564190676831954	RNA PCR Primer, Index 40 (95% over 23bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	536	0.2313955395919495	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGC	460	0.1985857242766731	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	416	0.17959056804151305	No Hit
ACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	405	0.17484177898272305	TruSeq Adapter, Index 8 (95% over 23bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	362	0.15627833084381665	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	361	0.155846622747563	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGT	293	0.12649047220231568	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTC	285	0.1230368074322866	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	259	0.11181239692969203	No Hit
CGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTG	249	0.10749531596715564	RNA PCR Primer, Index 46 (96% over 25bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCGAATAT	240	0.1036099431008729	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCT	239	0.10317823500461927	RNA PCR Primer, Index 46 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCACCG	30	2.1579726E-6	45.000004	1
CGACGGT	30	2.1579726E-6	45.000004	28
CACGACG	30	2.1579726E-6	45.000004	26
CAGATTA	30	2.1579726E-6	45.000004	20
TGATTGT	30	2.1579726E-6	45.000004	43
TTTGCGG	35	1.2063174E-7	45.000004	2
CGGTCTA	30	2.1579726E-6	45.000004	31
CGCCCAA	30	2.1579726E-6	45.000004	37
AAGCTGC	30	2.1579726E-6	45.000004	42
ATTAGTT	25	3.8811326E-5	45.0	17
GTTCTAT	25	3.8811326E-5	45.0	8
TGCTTAG	20	7.0216117E-4	45.0	1
ATTACGG	20	7.0216117E-4	45.0	2
ACATTGC	20	7.0216117E-4	45.0	32
ATAGCGG	20	7.0216117E-4	45.0	2
ACTTCCC	20	7.0216117E-4	45.0	20
CGACAGG	25	3.8811326E-5	45.0	2
GCACCGG	40	6.7739165E-9	45.0	2
TATTGAG	20	7.0216117E-4	45.0	1
TCGTCTA	20	7.0216117E-4	45.0	45

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content