##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935177.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	231638
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.598053859902087	31.0	31.0	34.0	30.0	34.0
2	31.710038940070284	31.0	31.0	34.0	30.0	34.0
3	31.715115827282226	31.0	31.0	34.0	30.0	34.0
4	35.515101149206956	37.0	35.0	37.0	33.0	37.0
5	32.90824476122225	37.0	35.0	37.0	28.0	37.0
6	33.95887548675088	37.0	35.0	37.0	26.0	37.0
7	35.322982412212156	37.0	35.0	37.0	32.0	37.0
8	35.249402084286686	37.0	35.0	37.0	33.0	37.0
9	37.34743867586493	39.0	37.0	39.0	34.0	39.0
10	36.76209430231655	39.0	37.0	39.0	32.0	39.0
11	36.6808943264922	39.0	37.0	39.0	32.0	39.0
12	37.03720460373514	39.0	37.0	39.0	33.0	39.0
13	36.97559554131878	39.0	37.0	39.0	33.0	39.0
14	37.92808606532607	40.0	37.0	41.0	33.0	41.0
15	38.05561695404035	40.0	37.0	41.0	33.0	41.0
16	38.05707181032473	40.0	37.0	41.0	33.0	41.0
17	37.999771194708984	39.0	37.0	41.0	33.0	41.0
18	37.88551964703546	39.0	37.0	40.0	33.0	41.0
19	37.6541025220387	39.0	37.0	40.0	33.0	41.0
20	37.42210690819296	39.0	35.0	40.0	33.0	41.0
21	37.33182811110439	39.0	35.0	40.0	33.0	41.0
22	37.32011587045304	39.0	35.0	40.0	33.0	41.0
23	37.04469473920514	39.0	35.0	40.0	32.0	41.0
24	36.761921619078045	39.0	35.0	40.0	32.0	41.0
25	36.56604270456488	38.0	35.0	40.0	31.0	41.0
26	36.91246254932265	39.0	35.0	40.0	32.0	41.0
27	36.81157668430914	39.0	35.0	40.0	32.0	41.0
28	36.92314732470493	39.0	35.0	40.0	32.0	41.0
29	37.00569422978958	39.0	35.0	40.0	33.0	41.0
30	36.65216415268652	39.0	35.0	40.0	31.0	41.0
31	36.50488261856863	39.0	35.0	40.0	31.0	41.0
32	36.2344088621038	39.0	35.0	40.0	30.0	41.0
33	35.95447206416909	39.0	35.0	40.0	30.0	41.0
34	35.80814460494392	39.0	35.0	40.0	28.0	41.0
35	35.629801673300584	39.0	35.0	40.0	26.0	41.0
36	35.416641483694384	39.0	35.0	40.0	25.0	41.0
37	35.41753511945363	39.0	35.0	40.0	25.0	41.0
38	35.29411409181568	39.0	35.0	40.0	24.0	41.0
39	35.04962052858339	38.0	35.0	40.0	23.0	41.0
40	34.919905196902064	38.0	35.0	40.0	22.0	41.0
41	34.83052866973467	38.0	35.0	40.0	22.0	41.0
42	34.99712482407895	39.0	35.0	40.0	22.0	41.0
43	34.8909073640767	38.0	35.0	40.0	21.0	41.0
44	34.76298793807579	38.0	35.0	40.0	21.0	41.0
45	34.7102677453613	38.0	35.0	40.0	20.0	41.0
46	34.55164955663579	38.0	34.0	40.0	20.0	41.0
47	34.46925374938482	38.0	34.0	40.0	20.0	41.0
48	34.45832290038767	38.0	34.0	40.0	20.0	41.0
49	34.44292387259431	38.0	34.0	40.0	20.0	41.0
50	34.32353499857536	38.0	34.0	40.0	20.0	41.0
51	33.3783360243138	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	5.0
11	6.0
12	3.0
13	7.0
14	10.0
15	20.0
16	33.0
17	74.0
18	127.0
19	202.0
20	357.0
21	650.0
22	1018.0
23	1591.0
24	2140.0
25	3019.0
26	3947.0
27	4423.0
28	4319.0
29	4308.0
30	4667.0
31	5491.0
32	6849.0
33	8967.0
34	13156.0
35	20276.0
36	25248.0
37	33048.0
38	48929.0
39	38738.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.955404553657	22.919814538201848	22.93621944585949	19.188561462281665
2	36.70511746777299	25.25967241989656	23.73272088344745	14.302489228882997
3	25.57266078968045	26.003073761645325	35.333149137878934	13.091116310795293
4	23.12487588392233	24.982084114005474	37.08372546818743	14.809314533884768
5	18.74519724742918	33.287716177829196	32.73426639843204	15.232820176309586
6	20.326975712102506	34.21675200096703	33.29850888023554	12.15776340669493
7	65.0091953824502	8.01897788791131	21.978259180272666	4.993567549365821
8	63.13558224470942	8.760220689178805	21.14506255450315	6.959134511608631
9	57.600221034545285	10.711541284245245	23.476286274272788	8.211951406936684
10	22.969029261174764	37.57544098982032	28.30623645515848	11.149293293846432
11	18.699436189226294	24.8719985494608	37.75028276880305	18.678282492509865
12	19.24641034717965	22.038698313748174	40.9147031143422	17.800188224729965
13	19.255044509104724	23.57169376354484	42.993809305899724	14.179452421450712
14	14.560650670442673	27.792503820616655	38.915462920591615	18.731382588349064
15	11.724760186152531	27.024063409285176	43.69403983802312	17.55713656653917
16	12.907640369887496	28.269972975073177	38.72680648252877	20.095580172510555
17	12.973691708614304	27.819269722584377	37.95189044975349	21.255148119047824
18	13.072552862656387	26.200364361633238	42.399347257358464	18.32773551835191
19	13.209836037265044	27.98461392344952	39.60446904221242	19.20108099707302
20	13.888481164575762	29.822395289201253	39.91918424438132	16.369939301841665
21	14.564104335212702	29.091945190340102	40.325421562955995	16.018528911491206
22	12.706896105129555	26.209861939750816	38.07579067337829	23.007451281741336
23	12.168987817197523	27.046080522194117	38.84897987376855	21.935951786839812
24	15.34247403275801	25.294640775693107	37.50464086203473	21.858244329514157
25	12.349441801431544	29.79088059817474	35.077577944896774	22.78209965549694
26	12.147834120481095	28.57734913960576	36.64338321000872	22.63143352990442
27	14.289969694091642	27.05039760315665	37.02371804280817	21.635914659943534
28	10.984812509173796	25.338243293414724	38.41727177751492	25.25967241989656
29	14.662965489254788	23.72365501342612	35.88141842012105	25.73196107719804
30	15.742667437985133	28.158592286239738	32.75067130608967	23.348068969685457
31	14.031808252531969	30.741501826125244	30.207910619155754	25.018779302187035
32	15.885131109748832	29.43860679163177	30.635733342543105	24.04052875607629
33	15.11712240651361	30.061993282622023	29.421770175877874	25.39911413498649
34	13.901432407463368	28.16506790768354	30.784240927654356	27.14925875719873
35	14.147506022327944	26.597535810186585	30.649979709719478	28.604978457765995
36	13.926903185142333	30.78337751146185	28.396463447275487	26.893255856120323
37	15.347654529913054	28.628722403059946	31.484471459777758	24.539151607249245
38	17.109455270724148	30.033068840173026	29.197713673922244	23.65976221518058
39	19.097471053972146	31.000958391973683	29.79562938723353	20.105941166820642
40	17.0503112615374	29.271967466477868	32.86507395159689	20.812647320387846
41	14.349113703278391	30.525647777998426	30.101278719381103	25.023959799342077
42	16.210639014324073	26.27159619751509	33.01012787193811	24.507636916222726
43	18.944214679802105	25.327018882912128	30.65516020687452	25.073606230411244
44	18.1127448864176	24.41784163220197	29.223616159697457	28.245797321682968
45	19.404415510408484	23.63429143750162	28.771186074823646	28.19010697726625
46	20.541966344036815	25.402567799756515	31.57642528427978	22.479040571926888
47	14.057710738307186	27.081480586086908	35.72902546214352	23.131783213462384
48	15.679638055932102	25.416814166932888	33.85023182724769	25.053315949887324
49	18.557404225558845	23.347637261589206	35.849040312902034	22.24591819994992
50	18.184408430395706	22.11424723059256	33.778999991365836	25.922344347645897
51	16.12947789222839	22.15439608354415	30.635733342543105	31.080392681684355
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2658.0
1	3016.5
2	3375.0
3	6488.5
4	9602.0
5	6237.5
6	2873.0
7	2808.0
8	2743.0
9	2713.0
10	2683.0
11	2825.5
12	2968.0
13	3106.5
14	3245.0
15	2904.5
16	2564.0
17	2474.0
18	2384.0
19	2343.0
20	2302.0
21	2226.5
22	2151.0
23	2278.0
24	2405.0
25	2463.0
26	2825.0
27	3129.0
28	3528.0
29	3927.0
30	3754.5
31	3582.0
32	3807.0
33	4032.0
34	4208.0
35	4384.0
36	4675.0
37	4966.0
38	5339.5
39	5713.0
40	6652.5
41	7592.0
42	9618.0
43	11644.0
44	13693.5
45	15743.0
46	20974.5
47	26206.0
48	27843.0
49	29480.0
50	28462.5
51	27445.0
52	21222.5
53	15000.0
54	11568.5
55	8137.0
56	6515.5
57	4894.0
58	4187.5
59	3481.0
60	3134.5
61	2788.0
62	2354.0
63	1920.0
64	1596.5
65	1273.0
66	1014.0
67	755.0
68	591.0
69	427.0
70	339.0
71	251.0
72	211.0
73	171.0
74	125.5
75	91.5
76	103.0
77	62.5
78	22.0
79	15.0
80	8.0
81	8.5
82	9.0
83	5.5
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	231638.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.219067661919624
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.195427513019	33.09828989254339
2	11.44228727908798	10.348191253604032
3	4.744666810700951	6.436482286390523
4	2.7830547681448357	5.033885674702774
5	1.875051486413188	4.239404001685046
6	1.3308834873576123	3.6108786278973257
7	1.0323561237375163	3.2677526987338674
8	0.788743855033053	2.8533009398930353
9	0.5880634405885312	2.3932512458526642
>10	2.0936655869288954	14.550479935225649
>50	0.07188551370767907	2.1887345782279897
>100	0.040934806416872804	3.4989053179180942
>500	0.00798727930085323	2.689868114084646
>1k	0.003993639650426615	3.188292820017752
>5k	9.984099126066537E-4	2.602282613223212
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5764	2.4883654668059645	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2146	0.9264455745603053	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCC	1959	0.8457161605608752	No Hit
GCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	1489	0.6428133553216657	RNA PCR Primer, Index 40 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	1468	0.6337474853003393	RNA PCR Primer, Index 46 (96% over 26bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCG	945	0.4079641509596871	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTC	930	0.40148852951588254	RNA PCR Primer, Index 46 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGCT	844	0.36436163323806975	RNA PCR Primer, Index 46 (96% over 27bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGC	776	0.33500548269282243	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	730	0.31514691026515507	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCG	628	0.27111268444728415	No Hit
TCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	569	0.24564190676831954	RNA PCR Primer, Index 40 (95% over 23bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	536	0.2313955395919495	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGC	460	0.1985857242766731	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	416	0.17959056804151305	No Hit
ACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	405	0.17484177898272305	TruSeq Adapter, Index 8 (95% over 23bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	362	0.15627833084381665	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	361	0.155846622747563	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGT	293	0.12649047220231568	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTC	285	0.1230368074322866	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	259	0.11181239692969203	No Hit
CGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTG	249	0.10749531596715564	RNA PCR Primer, Index 46 (96% over 25bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCGAATAT	240	0.1036099431008729	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCT	239	0.10317823500461927	RNA PCR Primer, Index 46 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.40019340522712166	0.0
2	0.0	0.0	0.0	2.261718716272805	0.0
3	0.0	0.0	0.0	2.9550419188561463	0.0
4	0.0	0.0	0.0	3.984234020324817	0.0
5	0.0	0.0	0.0	6.874951432839172	0.0
6	0.0	0.0	0.0	8.236558768423142	0.0
7	0.0	0.0	0.0	9.797615244476296	0.0
8	0.0	0.0	0.0	12.248853815004447	0.0
9	0.0	0.0	0.0	13.06694065740509	0.0
10	0.0	0.0	0.0	15.92873362747045	0.0
11	0.0	0.0	0.0	20.04981911430767	0.0
12	0.0	0.0	0.0	23.4482252480163	0.0
13	0.0	0.0	0.0	24.50116129477892	0.0
14	0.0	0.0	0.0	24.894447370465986	0.0
15	0.0	0.0	0.0	25.71382933715539	0.0
16	0.0	0.0	0.0	27.431164144052357	0.0
17	0.0	0.0	0.0	29.578048506721696	0.0
18	0.0	0.0	0.0	31.7922793324066	0.0
19	0.0	0.0	0.0	33.273038102556576	0.0
20	0.0	0.0	0.0	34.749479791744015	0.0
21	0.0	0.0	0.0	36.49228537631995	0.0
22	0.0	0.0	0.0	38.20962018321691	0.0
23	0.0	0.0	0.0	39.756862000189955	0.0
24	0.0	0.0	0.0	40.805480965990036	0.0
25	0.0	0.0	0.0	41.661558120860995	0.0
26	0.0	0.0	0.0	42.34797399390428	0.0
27	0.0	0.0	0.0	42.97395073347206	0.0
28	0.0	0.0	0.0	43.60899334306116	0.0
29	0.0	0.0	0.0	44.2259042126076	0.0
30	0.0	0.0	0.0	44.88814443226068	0.0
31	0.0	0.0	0.0	45.40230877489876	0.0
32	0.0	0.0	0.0	45.86898522694894	0.0
33	0.0	0.0	0.0	46.279107918389904	0.0
34	0.0	0.0	0.0	46.75010145140262	0.0
35	0.0	0.0	0.0	47.16108755903608	0.0
36	0.0	0.0	0.0	47.573800499054556	0.0
37	0.0	0.0	0.0	47.97269877999292	0.0
38	0.0	0.0	0.0	48.34871653182984	0.0
39	0.0	0.0	0.0	48.740275775131884	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCACCG	30	2.1579726E-6	45.000004	1
CGACGGT	30	2.1579726E-6	45.000004	28
CACGACG	30	2.1579726E-6	45.000004	26
CAGATTA	30	2.1579726E-6	45.000004	20
TGATTGT	30	2.1579726E-6	45.000004	43
TTTGCGG	35	1.2063174E-7	45.000004	2
CGGTCTA	30	2.1579726E-6	45.000004	31
CGCCCAA	30	2.1579726E-6	45.000004	37
AAGCTGC	30	2.1579726E-6	45.000004	42
ATTAGTT	25	3.8811326E-5	45.0	17
GTTCTAT	25	3.8811326E-5	45.0	8
TGCTTAG	20	7.0216117E-4	45.0	1
ATTACGG	20	7.0216117E-4	45.0	2
ACATTGC	20	7.0216117E-4	45.0	32
ATAGCGG	20	7.0216117E-4	45.0	2
ACTTCCC	20	7.0216117E-4	45.0	20
CGACAGG	25	3.8811326E-5	45.0	2
GCACCGG	40	6.7739165E-9	45.0	2
TATTGAG	20	7.0216117E-4	45.0	1
TCGTCTA	20	7.0216117E-4	45.0	45
>>END_MODULE