FastQCFastQC Report
Sat 14 Jan 2017
SRR2935176.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935176.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912253
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190712.090538480004999No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC115911.2705905050462973RNA PCR Primer, Index 21 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCG115111.2618210079879157No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGC82940.9091776075277364No Hit
GCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC69480.761630819520462TruSeq Adapter, Index 21 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCC47360.5191542258562044No Hit
CCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC35330.38728291384078756RNA PCR Primer, Index 21 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCT27750.3041919292126197RNA PCR Primer, Index 21 (96% over 27bp)
GAACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT26800.29377815145579134RNA PCR Primer, Index 14 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26410.2895030216398302No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCT26320.2885164532207622RNA PCR Primer, Index 21 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCGATCT24020.2633041491779145No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTC20660.22647226153271077No Hit
TCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC19800.21704505219495032TruSeq Adapter, Index 21 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTA17830.19545016568868503No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCG16030.17571879730732592No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13820.15149306168354612No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13800.15127382425708658No Hit
ACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC13340.146231363448517RNA PCR Primer, Index 14 (96% over 25bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11610.12726732605976632No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGC10760.11794973543523561No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTC9640.10567243955350106No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9610.10534358341381174No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCG253.89017E-545.0000041
CGACAGG253.89017E-545.0000042
ACAATCG253.89017E-545.0000044
GTCAACG253.89017E-545.0000041
GTCGGGC253.89017E-545.0000044
AAATGCG253.89017E-545.0000041
CGACGAA207.032521E-445.019
TAGAACG207.032521E-445.01
ACGGGTC351.2115561E-745.05
TCAGGCG207.032521E-445.01
TTACGAG351.2115561E-745.01
CGTTTTT68700.043.820961
CGTTATT4100.043.3536571
CCGATGA7550.042.61589418
CGATGAA7800.042.11538319
CGACGGT700.041.78571328
CGGTCTA700.041.78571331
GCGTAAG700.041.7857131
ACGGGTG603.6379788E-1241.2500045
TACGGCT9950.040.7035187
Produced by FastQC (version 0.11.5)