##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935176.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	912253
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.587836926817452	31.0	31.0	34.0	30.0	34.0
2	31.6787809960614	31.0	31.0	34.0	30.0	34.0
3	31.70521116400823	31.0	31.0	34.0	30.0	34.0
4	35.52760034771056	37.0	35.0	37.0	33.0	37.0
5	32.942998269120515	37.0	35.0	37.0	28.0	37.0
6	33.96598421709767	36.0	35.0	37.0	27.0	37.0
7	35.335558227816186	37.0	35.0	37.0	32.0	37.0
8	35.2152615557307	37.0	35.0	37.0	32.0	37.0
9	37.2489945223529	39.0	37.0	39.0	34.0	39.0
10	36.71237584310493	39.0	35.0	39.0	32.0	39.0
11	36.65508691119678	39.0	37.0	39.0	32.0	39.0
12	36.95905741060868	39.0	37.0	39.0	33.0	39.0
13	36.85938878797877	39.0	37.0	39.0	33.0	39.0
14	37.724111622543305	39.0	37.0	41.0	32.0	41.0
15	37.83644668748691	40.0	37.0	41.0	33.0	41.0
16	37.89500938884279	40.0	37.0	41.0	33.0	41.0
17	37.82512088203602	39.0	37.0	41.0	33.0	41.0
18	37.77002761295386	39.0	37.0	40.0	33.0	41.0
19	37.612896860848906	39.0	37.0	40.0	33.0	41.0
20	37.31479205878194	39.0	35.0	40.0	32.0	41.0
21	37.32164870929446	39.0	35.0	40.0	32.0	41.0
22	37.37242957819815	39.0	35.0	40.0	33.0	41.0
23	37.02387495574145	39.0	35.0	40.0	32.0	41.0
24	36.77653019502265	39.0	35.0	40.0	31.0	41.0
25	36.60106680931715	38.0	35.0	40.0	31.0	41.0
26	36.93082949576488	39.0	35.0	40.0	32.0	41.0
27	36.802969132466544	39.0	35.0	40.0	32.0	41.0
28	36.918898595016955	39.0	35.0	40.0	32.0	41.0
29	37.039050570400974	39.0	35.0	40.0	32.0	41.0
30	36.73905265315653	39.0	35.0	40.0	31.0	41.0
31	36.584518768367985	39.0	35.0	40.0	31.0	41.0
32	36.35568203119091	39.0	35.0	40.0	30.0	41.0
33	36.19904127473409	39.0	35.0	40.0	30.0	41.0
34	35.927042169222794	39.0	35.0	40.0	28.0	41.0
35	35.83299698658157	39.0	35.0	40.0	27.0	41.0
36	35.70930980769589	39.0	35.0	40.0	26.0	41.0
37	35.66446259973932	39.0	35.0	40.0	25.0	41.0
38	35.653886586286916	39.0	35.0	40.0	25.0	41.0
39	35.4647822479071	39.0	35.0	40.0	24.0	41.0
40	35.30595130955996	39.0	35.0	40.0	24.0	41.0
41	35.12056304555863	39.0	35.0	40.0	23.0	41.0
42	35.386089166053715	39.0	35.0	40.0	24.0	41.0
43	35.394228355511025	39.0	35.0	40.0	24.0	41.0
44	35.33509234828496	39.0	35.0	40.0	23.0	41.0
45	35.31691427707007	39.0	35.0	40.0	23.0	41.0
46	35.14647033224336	39.0	35.0	40.0	23.0	41.0
47	35.02657267227403	39.0	35.0	40.0	23.0	41.0
48	35.02500950942337	39.0	35.0	40.0	23.0	41.0
49	35.08235653924953	39.0	35.0	40.0	23.0	41.0
50	34.96920152633096	39.0	35.0	40.0	23.0	41.0
51	33.992522907570596	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	13.0
10	22.0
11	21.0
12	23.0
13	27.0
14	35.0
15	66.0
16	111.0
17	224.0
18	404.0
19	737.0
20	1578.0
21	2839.0
22	4541.0
23	6194.0
24	8311.0
25	11097.0
26	14145.0
27	15356.0
28	15461.0
29	16166.0
30	17900.0
31	21629.0
32	27363.0
33	36761.0
34	52435.0
35	68744.0
36	91267.0
37	126813.0
38	198573.0
39	173349.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.76544555074086	20.477762199740642	21.895241780514834	16.861550469003664
2	34.7133963933251	28.546357205731304	22.854405521275346	13.88584087966825
3	25.73935081605651	28.338848981587343	32.989313271647234	12.932486930708913
4	22.733824936722595	22.802336632491205	39.06065532259143	15.403183108194765
5	22.541553713717576	30.321961122627165	31.02856334810628	16.107921815548977
6	20.57104772469918	34.934058863056634	32.28161485903582	12.213278553208376
7	65.92403642410603	5.605024044864747	23.647496911492755	4.823442619536467
8	63.04829910123617	9.655435498704854	20.106373999318173	7.189891400740803
9	57.39811214652075	8.323458514249884	21.644872639498033	12.633556699731324
10	25.660973436097223	33.13981976491171	27.739125001507258	13.460081797483811
11	22.101434580100037	22.519410733645163	36.152032385752634	19.227122300502163
12	20.97203297769369	18.979932102169027	39.41560071602944	20.632434204107852
13	21.760849238095133	20.72034841211813	43.327454116347106	14.191348233439626
14	16.227406212969427	26.21016318937838	38.174716882268406	19.387713715383782
15	12.845340053691245	24.16796656190772	43.96258494080041	19.024108443600625
16	14.852020218075468	26.212026707503288	35.81363941801233	23.122313656408913
17	14.914174028476749	26.249406688714643	37.38151587333777	21.454903409470838
18	14.610968667683197	24.486957017406354	40.66163662931226	20.240437685598184
19	14.423739905486746	26.51128579462057	37.27200677882123	21.792967521071457
20	14.953636765239468	28.038822563477456	40.31502225807972	16.692518413203356
21	14.962515881011079	28.549865004554658	39.48060461297469	17.007014501459572
22	13.190200525512111	23.34626468753734	38.55761504758	24.905919739370546
23	13.578360389058735	25.859602544469574	39.05879180446652	21.503245262005166
24	16.467251957516172	24.106908938638732	35.756747305846076	23.66909179799902
25	12.922621246518235	30.326674727296044	33.69745015911156	23.053253867074154
26	12.765208774320284	25.34143488703243	37.59209342145216	24.30126291719512
27	16.179941310140936	24.757824857797125	37.59044914075372	21.471784691308223
28	10.677191524719568	24.496274608030884	39.59899282326285	25.2275410439867
29	14.751499858043765	21.358219704402178	36.320516347986796	27.569764089567254
30	16.870210347348817	25.734856448814092	37.48806526259711	19.906867941239987
31	14.243965215789917	27.33770127365983	34.27393497198693	24.144398538563316
32	16.38712067814521	25.857629407631432	32.292138255505876	25.463111658717487
33	18.256119738712833	23.18205585511914	32.01337786776256	26.54844653840546
34	14.845223857855222	24.85571436871131	32.949631297458055	27.349430475975417
35	14.136538876824742	23.0806585453816	34.47453721719742	28.30826536059624
36	15.786410129646052	23.65001814189704	34.95313251915861	25.610439209298296
37	13.41656316833159	24.05900556095732	38.91332777201061	23.61110349870047
38	17.579553040658677	22.899678049839245	33.07810442936335	26.442664480138735
39	20.68099529406864	23.459171962164007	29.503218953513993	26.356613790253363
40	20.046083707041795	22.923465310610105	34.90237905493323	22.128071927414872
41	15.614089512448851	25.66809865245716	31.422094528601168	27.29571730649283
42	16.13181869503307	20.42185665599346	38.0609326579359	25.385391991037576
43	18.75170594122464	19.477381822805732	33.50649436066529	28.264417875304325
44	19.04449752426136	19.835067684074485	32.47311875104823	28.647316040615927
45	19.526052531479753	18.116246260631645	32.52891467608218	29.82878653180642
46	22.05309272756571	21.646078445343562	33.125898188331526	23.174930638759204
47	14.576658010442278	21.68565080081951	40.80929303603277	22.928398152705444
48	16.923813898118176	21.379047259915833	35.39171699079093	26.30542185117506
49	18.87623279945366	18.091582050154948	38.56309598324149	24.469089167149903
50	19.048005323084716	17.590843768121346	34.980427578752824	28.380723330041118
51	17.919151814244515	17.933621484390844	31.42143681632179	32.72578988504286
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3824.0
1	5970.5
2	8117.0
3	19420.5
4	30724.0
5	19603.5
6	8483.0
7	8349.5
8	8216.0
9	8150.0
10	8084.0
11	8183.0
12	8282.0
13	8077.0
14	7872.0
15	7515.0
16	7158.0
17	7204.0
18	7250.0
19	6996.5
20	6743.0
21	7174.5
22	7606.0
23	7646.5
24	7687.0
25	8406.0
26	9808.0
27	10491.0
28	11301.5
29	12112.0
30	12988.0
31	13864.0
32	14971.0
33	16078.0
34	17385.5
35	18693.0
36	20298.0
37	21903.0
38	23702.0
39	25501.0
40	28891.0
41	32281.0
42	38845.0
43	45409.0
44	54589.5
45	63770.0
46	96542.0
47	129314.0
48	125034.0
49	120754.0
50	114732.0
51	108710.0
52	85223.0
53	61736.0
54	48111.5
55	34487.0
56	27684.5
57	20882.0
58	18046.0
59	15210.0
60	13392.0
61	11574.0
62	9632.5
63	7691.0
64	6418.5
65	5146.0
66	3998.5
67	2851.0
68	2423.0
69	1995.0
70	1546.5
71	1098.0
72	868.5
73	639.0
74	531.0
75	328.0
76	233.0
77	180.0
78	127.0
79	96.0
80	65.0
81	46.5
82	28.0
83	18.5
84	9.0
85	6.5
86	4.0
87	2.5
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	912253.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.822440681200646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.52369429011533	26.67378243907016
2	11.573715105279167	8.754923060610743
3	5.056743761666215	5.737751728969555
4	2.9269651746681773	4.4281986677930885
5	1.9927969483064252	3.768622218349872
6	1.438599725010445	3.264681165791947
7	1.1389229302416113	3.0153791478665792
8	0.9217252504001126	2.7889518886098457
9	0.7355539813065324	2.503840214320856
>10	3.5241125840139476	20.71217894921957
>50	0.104256833698281	2.731150636711942
>100	0.05182867069460117	3.85598616090959
>500	0.004793403069922254	1.2742997462566417
>1k	0.004793403069922254	3.984481262437182
>5k	5.991753837402817E-4	1.7270920089315074
>10k+	8.987630756104226E-4	4.778680704150929
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19071	2.090538480004999	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	11591	1.2705905050462973	RNA PCR Primer, Index 21 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCG	11511	1.2618210079879157	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGC	8294	0.9091776075277364	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	6948	0.761630819520462	TruSeq Adapter, Index 21 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCC	4736	0.5191542258562044	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	3533	0.38728291384078756	RNA PCR Primer, Index 21 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCT	2775	0.3041919292126197	RNA PCR Primer, Index 21 (96% over 27bp)
GAACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	2680	0.29377815145579134	RNA PCR Primer, Index 14 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2641	0.2895030216398302	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCT	2632	0.2885164532207622	RNA PCR Primer, Index 21 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCGATCT	2402	0.2633041491779145	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTC	2066	0.22647226153271077	No Hit
TCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	1980	0.21704505219495032	TruSeq Adapter, Index 21 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTA	1783	0.19545016568868503	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCG	1603	0.17571879730732592	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1382	0.15149306168354612	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1380	0.15127382425708658	No Hit
ACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	1334	0.146231363448517	RNA PCR Primer, Index 14 (96% over 25bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1161	0.12726732605976632	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGC	1076	0.11794973543523561	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTC	964	0.10567243955350106	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	961	0.10534358341381174	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0961871322977288E-4	0.0	0.0	0.3262252905718041	0.0
2	1.0961871322977288E-4	0.0	0.0	1.955268987879459	0.0
3	1.0961871322977288E-4	0.0	0.0	2.5814110778479216	0.0
4	1.0961871322977288E-4	0.0	0.0	3.7691298356925107	0.0
5	1.0961871322977288E-4	0.0	0.0	7.482682983777527	0.0
6	1.0961871322977288E-4	0.0	0.0	9.026004847339498	0.0
7	1.0961871322977288E-4	0.0	0.0	10.75863822864929	0.0
8	1.0961871322977288E-4	0.0	0.0	13.144051047242376	0.0
9	1.0961871322977288E-4	0.0	0.0	13.896035419998618	0.0
10	1.0961871322977288E-4	0.0	0.0	17.696516207674843	0.0
11	1.0961871322977288E-4	0.0	0.0	21.485596649175175	0.0
12	1.0961871322977288E-4	0.0	0.0	25.365331766516526	0.0
13	1.0961871322977288E-4	0.0	0.0	26.510628082341192	0.0
14	1.0961871322977288E-4	0.0	0.0	27.00007563691213	0.0
15	1.0961871322977288E-4	0.0	0.0	28.130025332884628	0.0
16	1.0961871322977288E-4	0.0	0.0	29.960109750255686	0.0
17	1.0961871322977288E-4	0.0	0.0	32.167063303710705	0.0
18	1.0961871322977288E-4	0.0	0.0	34.3069302046691	0.0
19	2.1923742645954577E-4	0.0	0.0	36.07957441630776	0.0
20	2.1923742645954577E-4	0.0	0.0	37.8249235683522	0.0
21	2.1923742645954577E-4	0.0	0.0	39.67375278568555	0.0
22	2.1923742645954577E-4	0.0	0.0	41.66497671150437	0.0
23	2.1923742645954577E-4	0.0	0.0	43.23910143348391	0.0
24	2.1923742645954577E-4	0.0	0.0	44.27379246765974	0.0
25	2.1923742645954577E-4	0.0	0.0	45.11676037239669	0.0
26	2.1923742645954577E-4	0.0	0.0	45.843532441110085	0.0
27	2.1923742645954577E-4	0.0	0.0	46.543996018648336	0.0
28	2.1923742645954577E-4	0.0	0.0	47.1701381086168	0.0
29	2.1923742645954577E-4	0.0	0.0	47.77698730505682	0.0
30	3.2885613968931864E-4	0.0	0.0	48.41222774822335	0.0
31	5.480935661488644E-4	0.0	0.0	48.92173552731534	0.0
32	5.480935661488644E-4	0.0	0.0	49.37139148898387	0.0
33	5.480935661488644E-4	0.0	0.0	49.798137139587375	0.0
34	5.480935661488644E-4	0.0	0.0	50.19988972357449	0.0
35	6.577122793786373E-4	0.0	0.0	50.61589274028148	0.0
36	6.577122793786373E-4	0.0	0.0	50.974126695116375	0.0
37	6.577122793786373E-4	0.0	0.0	51.33455302421587	0.0
38	6.577122793786373E-4	0.0	0.0	51.73137276610765	0.0
39	7.673309926084102E-4	0.0	0.0	52.201088952297226	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCG	25	3.89017E-5	45.000004	1
CGACAGG	25	3.89017E-5	45.000004	2
ACAATCG	25	3.89017E-5	45.000004	4
GTCAACG	25	3.89017E-5	45.000004	1
GTCGGGC	25	3.89017E-5	45.000004	4
AAATGCG	25	3.89017E-5	45.000004	1
CGACGAA	20	7.032521E-4	45.0	19
TAGAACG	20	7.032521E-4	45.0	1
ACGGGTC	35	1.2115561E-7	45.0	5
TCAGGCG	20	7.032521E-4	45.0	1
TTACGAG	35	1.2115561E-7	45.0	1
CGTTTTT	6870	0.0	43.82096	1
CGTTATT	410	0.0	43.353657	1
CCGATGA	755	0.0	42.615894	18
CGATGAA	780	0.0	42.115383	19
CGACGGT	70	0.0	41.785713	28
CGGTCTA	70	0.0	41.785713	31
GCGTAAG	70	0.0	41.785713	1
ACGGGTG	60	3.6379788E-12	41.250004	5
TACGGCT	995	0.0	40.703518	7
>>END_MODULE