##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935175.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	417152
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.730724052623504	31.0	31.0	34.0	30.0	34.0
2	31.83912818349187	33.0	31.0	34.0	30.0	34.0
3	31.826801741331696	33.0	31.0	34.0	30.0	34.0
4	35.627910210187174	37.0	35.0	37.0	33.0	37.0
5	33.04381856014115	37.0	35.0	37.0	28.0	37.0
6	34.10497132939552	37.0	35.0	37.0	28.0	37.0
7	35.42712967934949	37.0	35.0	37.0	32.0	37.0
8	35.40666471693771	37.0	35.0	37.0	33.0	37.0
9	37.49788566277999	39.0	37.0	39.0	35.0	39.0
10	36.96882910785517	39.0	37.0	39.0	33.0	39.0
11	36.808861038662165	39.0	37.0	39.0	32.0	39.0
12	37.18136794262043	39.0	37.0	39.0	34.0	39.0
13	37.13202381865603	39.0	37.0	39.0	33.0	39.0
14	38.14643822875115	40.0	37.0	41.0	33.0	41.0
15	38.27975174516723	40.0	38.0	41.0	34.0	41.0
16	38.23679857701749	40.0	38.0	41.0	33.0	41.0
17	38.180490564590364	40.0	37.0	41.0	33.0	41.0
18	38.05871001457502	39.0	37.0	41.0	34.0	41.0
19	37.85732299018104	39.0	37.0	41.0	34.0	41.0
20	37.64443656029457	39.0	35.0	41.0	33.0	41.0
21	37.58138759972384	39.0	35.0	40.0	33.0	41.0
22	37.5268894791347	39.0	35.0	40.0	33.0	41.0
23	37.287784788278614	39.0	35.0	40.0	33.0	41.0
24	37.02420220926665	39.0	35.0	40.0	32.0	41.0
25	36.78930941239644	38.0	35.0	40.0	32.0	41.0
26	37.01297368824793	39.0	35.0	40.0	33.0	41.0
27	36.911104345658174	39.0	35.0	40.0	32.0	41.0
28	36.979189839674746	39.0	35.0	40.0	33.0	41.0
29	36.94492894676281	39.0	35.0	40.0	33.0	41.0
30	36.6270472154035	39.0	35.0	40.0	31.0	41.0
31	36.48499108238723	38.0	35.0	40.0	31.0	41.0
32	36.165623082233815	38.0	35.0	40.0	30.0	41.0
33	35.95971732126419	38.0	35.0	40.0	30.0	41.0
34	35.78697692927278	38.0	35.0	40.0	29.0	41.0
35	35.499930480975756	38.0	35.0	40.0	26.0	41.0
36	35.32264018870819	38.0	35.0	40.0	25.0	41.0
37	35.31000210954281	38.0	35.0	40.0	25.0	41.0
38	35.21136899739184	38.0	35.0	40.0	25.0	41.0
39	35.09150861077018	38.0	35.0	40.0	24.0	41.0
40	34.97024346041731	38.0	35.0	40.0	23.0	41.0
41	34.90246720619822	38.0	35.0	40.0	23.0	41.0
42	34.964269618748084	38.0	35.0	40.0	23.0	41.0
43	34.85940136928506	38.0	35.0	40.0	22.0	41.0
44	34.746864452285976	38.0	35.0	40.0	22.0	41.0
45	34.748777424056456	38.0	35.0	40.0	22.0	41.0
46	34.63567476603252	38.0	34.0	40.0	22.0	41.0
47	34.54272303620743	38.0	34.0	40.0	21.0	41.0
48	34.51661984120896	38.0	34.0	40.0	21.0	41.0
49	34.50208077631175	38.0	34.0	40.0	20.0	41.0
50	34.42428419377109	38.0	34.0	40.0	20.0	41.0
51	33.52607203129794	37.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	8.0
10	8.0
11	9.0
12	19.0
13	24.0
14	19.0
15	38.0
16	61.0
17	92.0
18	177.0
19	377.0
20	684.0
21	994.0
22	1534.0
23	2449.0
24	3667.0
25	5352.0
26	6924.0
27	7761.0
28	7558.0
29	7343.0
30	7730.0
31	9229.0
32	11538.0
33	15661.0
34	23406.0
35	36985.0
36	48425.0
37	61298.0
38	87617.0
39	70139.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.95657218471924	24.08810217858239	24.133888846271862	18.82143679042651
2	37.13778191162934	23.955296870205583	24.58264613378337	14.324275084381712
3	26.27291730592206	24.255666999079473	36.32896402270635	13.142451672292113
4	24.203887312058914	24.959487189321877	35.20371471310218	15.63291078551703
5	18.14039007364222	33.398377569806684	33.19749156182878	15.263740794722308
6	20.34342397974839	32.71517336606321	34.554311138386005	12.387091515802394
7	65.62092474685485	7.8920872967167846	21.623772629640996	4.863215326787358
8	63.915790886775085	7.289189552009819	21.843596578705125	6.951422982509972
9	58.03615948143602	10.874213715863762	24.16193617674133	6.927690625958884
10	24.728636084688553	35.54315932801473	28.960187173979747	10.768017413316969
11	18.095322568272476	25.873542497698683	37.5664026541884	18.464732279840444
12	18.94681075483277	21.614902961030992	41.46785823872354	17.970428045412703
13	19.33252147898128	24.3498772629641	41.83654878797177	14.481052470082847
14	14.429752224608775	27.93178505676588	39.650055615219394	17.988407103405955
15	12.790781297944154	28.03390610616754	41.880657410248546	17.29465518563977
16	14.026781604786745	29.164189552009816	37.98591400736422	18.823114835839213
17	13.94144100951212	28.754746471310217	36.06191508131328	21.241897437864377
18	12.97201020251611	28.520539275851487	41.16077592819883	17.34667459343357
19	14.293351104633324	28.80269062595888	38.360118134397055	18.54384013501074
20	14.298385240871433	31.578657180116597	38.335666615526236	15.78729096348573
21	15.514488723534829	29.026350107394904	39.537866293341516	15.92129487572875
22	13.063823258668302	28.80340978827861	36.51474762196993	21.618019331083154
23	13.062145213255599	27.984044185332923	37.7821513501074	21.17165925130408
24	15.530310294568887	27.304915234734583	37.14185716477447	20.02291730592206
25	12.957387235348266	30.918226449831238	34.45602562135625	21.668360693464255
26	12.284730745627494	33.31663278613072	35.39764881865603	19.000987649585763
27	15.585925513961337	30.91750728751151	36.16475529303467	17.331811905492483
28	13.058789122430191	29.684863071494323	36.30331389996931	20.953033906106167
29	13.833566661552624	30.00489030377416	36.301156413010126	19.860386621663086
30	16.138481896287203	33.530703436637005	32.8700329855784	17.46078168149739
31	16.319950521632403	33.481320957348885	30.150400813132862	20.048327707885853
32	14.489922138692851	33.73566469776005	30.8853847038969	20.8890284596502
33	17.181506980668917	32.4373369898742	29.277098036207427	21.104057993249466
34	15.514968165081314	29.76972422522246	30.506386161399202	24.208921448297023
35	14.335302239950906	32.638222997852104	28.89977753912243	24.126697223074565
36	18.767499616446763	34.79594967781528	27.611997545259282	18.824553160478676
37	18.480074409328015	31.127742405645904	31.097058146670758	19.295125038355323
38	17.397735118134396	31.037367674133172	30.401148741945384	21.16374846578705
39	19.61850836146057	29.432676818042346	30.69864222154035	20.250172598956734
40	17.376879410862227	29.634281988339982	29.742635777845965	23.246202822951826
41	17.043907256827246	30.439264344891072	25.993882325866828	26.52294607241485
42	20.844680116600184	28.95898857011353	28.630091669223688	21.566239644062595
43	22.002291730592205	27.20878720466401	31.133495704203746	19.655425360540043
44	18.365487879717705	28.409308837066582	29.834688554771404	23.39051472844431
45	18.837737803007055	27.05680423442774	29.543188094507517	24.562269868057687
46	20.188804081006445	28.091199370972692	30.332348880024547	21.38764766799632
47	16.22885662779994	28.737966017183187	33.41923327707886	21.61394407793802
48	17.541567582080393	26.679004295796254	32.28847038969009	23.490957732433262
49	20.103703206505063	24.261660018410556	34.54016761276465	21.09446916231973
50	18.300523550168762	23.286475912856705	32.498945228597734	25.914055308376803
51	17.427220773243327	23.288153958269408	29.03378145136545	30.250843817121815
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4224.0
1	5357.5
2	6491.0
3	12208.5
4	17926.0
5	11687.0
6	5448.0
7	5278.0
8	5108.0
9	5791.0
10	6474.0
11	6878.5
12	7283.0
13	7214.0
14	7145.0
15	6415.5
16	5686.0
17	5257.0
18	4828.0
19	4487.0
20	4146.0
21	4009.5
22	3873.0
23	4192.0
24	4511.0
25	5061.0
26	5865.5
27	6120.0
28	6237.0
29	6354.0
30	6584.5
31	6815.0
32	7370.0
33	7925.0
34	8117.0
35	8309.0
36	9114.5
37	9920.0
38	11341.5
39	12763.0
40	13924.0
41	15085.0
42	18543.0
43	22001.0
44	24792.0
45	27583.0
46	33581.0
47	39579.0
48	45911.0
49	52243.0
50	49560.0
51	46877.0
52	35760.0
53	24643.0
54	18714.0
55	12785.0
56	10404.5
57	8024.0
58	7150.0
59	6276.0
60	5804.0
61	5332.0
62	4586.5
63	3841.0
64	3042.0
65	2243.0
66	1848.5
67	1454.0
68	1138.0
69	822.0
70	710.5
71	599.0
72	503.5
73	408.0
74	295.0
75	146.0
76	110.0
77	80.5
78	51.0
79	38.0
80	25.0
81	24.5
82	24.0
83	12.5
84	1.0
85	0.5
86	0.0
87	0.5
88	1.0
89	1.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	417152.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.67683777363965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.58023812804586	30.66591647808342
2	10.830786614942605	9.027858734237414
3	4.471810561502883	5.591127699786122
4	2.460766232611675	4.1022782030162865
5	1.6531313017239244	3.4448642540236865
6	1.2274356262317374	3.0693381283227548
7	0.9550774901039154	2.7863226731421067
8	0.762167097500934	2.5411771583161777
9	0.6307552898137803	2.3659097299590583
>10	3.2741940660185973	21.515749184596434
>50	0.09136781009902231	2.6051518311545125
>100	0.051794491075241936	3.7979771458940976
>500	0.005237645164912105	1.6633582774469458
>1k	0.004655684591032982	4.02329937391221
>5k	0.0	0.0
>10k+	5.819605738791228E-4	2.799671128108792
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11543	2.7670968855477143	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3289	0.7884416231972998	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCC	2944	0.7057379564283522	No Hit
CCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	2487	0.5961855630561521	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	2394	0.5738915311445228	No Hit
CTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCT	1634	0.391703743479595	No Hit
TCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	1362	0.3264996931574103	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1259	0.3018084535133476	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1219	0.2922196225836146	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	930	0.22294031911629336	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG	891	0.21359120895980363	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	854	0.20472154034980056	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	828	0.19848880024547405	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	823	0.19729019637925743	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	791	0.189619131635471	No Hit
ACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	622	0.14910632095734888	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC	567	0.13592167842896594	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	552	0.13232586683031605	No Hit
GCCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTG	439	0.1052374194538202	No Hit
AGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTG	438	0.10499769868057686	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTG	437	0.10475797790733354	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.424066047867444	0.0
2	0.0	0.0	0.0	2.1663566277999387	0.0
3	0.0	0.0	0.0	2.9535996471310217	0.0
4	0.0	0.0	0.0	4.025391224301933	0.0
5	0.0	0.0	0.0	6.826049018103713	0.0
6	0.0	0.0	0.0	8.24495627493096	0.0
7	0.0	0.0	0.0	9.78516224301933	0.0
8	0.0	0.0	0.0	12.004736882479287	0.0
9	0.0	0.0	0.0	12.797733200368212	0.0
10	0.0	0.0	0.0	15.051108468855476	0.0
11	0.0	0.0	0.0	19.075301089291195	0.0
12	0.0	0.0	0.0	21.908321187480823	0.0
13	0.0	0.0	0.0	22.95254487572875	0.0
14	0.0	0.0	0.0	23.36102907333538	0.0
15	0.0	0.0	0.0	24.169367520711877	0.0
16	0.0	0.0	0.0	25.77333921448297	0.0
17	0.0	0.0	0.0	27.923394829702364	0.0
18	0.0	0.0	0.0	29.92026887081927	0.0
19	0.0	0.0	0.0	31.28667727830623	0.0
20	0.0	0.0	0.0	32.70438593126726	0.0
21	0.0	0.0	0.0	34.28102945688862	0.0
22	0.0	0.0	0.0	35.87613148204971	0.0
23	0.0	0.0	0.0	37.21736920834612	0.0
24	0.0	0.0	0.0	38.16858123657563	0.0
25	0.0	0.0	0.0	38.92825636698374	0.0
26	0.0	0.0	0.0	39.57694077938018	0.0
27	0.0	0.0	0.0	40.172646900889845	0.0
28	0.0	0.0	0.0	40.72256635471003	0.0
29	0.0011986038662166309	0.0	0.0	41.35279226756674	0.0
30	0.0011986038662166309	0.0	0.0	41.96408023933722	0.0
31	0.0011986038662166309	0.0	0.0	42.4552681037128	0.0
32	0.0011986038662166309	0.0	0.0	42.89899125498619	0.0
33	0.0011986038662166309	0.0	0.0	43.29692773857011	0.0
34	0.0011986038662166309	0.0	0.0	43.72770596808837	0.0
35	0.0011986038662166309	0.0	0.0	44.13163547100338	0.0
36	0.0011986038662166309	0.0	0.0	44.53556497391838	0.0
37	0.0011986038662166309	0.0	0.0	44.92127569806689	0.0
38	0.0011986038662166309	0.0	0.0	45.29212373427432	0.0
39	0.0011986038662166309	0.0	0.0	45.64523243326174	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	30	2.1621636E-6	45.000004	1
ATGTACG	35	1.209446E-7	45.000004	1
AAGTTCG	30	2.1621636E-6	45.000004	1
GACGGGC	30	2.1621636E-6	45.000004	4
CGGTCTA	35	1.209446E-7	45.000004	31
TAGTACG	30	2.1621636E-6	45.000004	1
CTCACGA	40	6.7975634E-9	45.0	24
CCTTAAT	25	3.8865182E-5	45.0	42
GGCGTAG	20	7.0281135E-4	45.0	1
GGCCGAT	20	7.0281135E-4	45.0	8
GGGCGCG	20	7.0281135E-4	45.0	7
TATTCGT	25	3.8865182E-5	45.0	14
CGGGCAC	25	3.8865182E-5	45.0	6
TACCTTG	20	7.0281135E-4	45.0	11
ACTAATC	25	3.8865182E-5	45.0	10
GACGACG	20	7.0281135E-4	45.0	1
CTGGCGG	20	7.0281135E-4	45.0	2
TGCACGG	55	1.8189894E-12	45.0	2
GACTAAT	55	1.8189894E-12	45.0	9
TGATCTA	25	3.8865182E-5	45.0	41
>>END_MODULE