SRR2935174.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935174.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	307700
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8468	2.7520311992200197	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCC	2240	0.7279818004549886	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	1995	0.6483587910302242	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1943	0.631459213519662	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	1681	0.5463113422164446	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCG	1603	0.5209619759506012	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTC	1512	0.49138771530711733	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGC	1447	0.47026324341891457	No Hit
CTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGCT	1113	0.36171595710107246	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	861	0.27981800454988626	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	730	0.23724406889827754	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCG	717	0.23301917452063697	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	638	0.2073448163795905	No Hit
ACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	601	0.19532011699707508	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	587	0.19077023074423138	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	557	0.1810204744881378	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	527	0.17127071823204418	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGC	455	0.14787130321741956	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTGTCGAT	394	0.12804679883002926	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCT	379	0.12317192070198245	No Hit
ACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTG	374	0.12154696132596686	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGT	349	0.11342216444588885	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTG	341	0.1108222294442639	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	326	0.1059473513162171	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCT	315	0.10237244068898278	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTG	313	0.10172245693857652	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTC	309	0.10042248943776405	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	25	3.884125E-5	45.000004	13
AAGTACG	25	3.884125E-5	45.000004	1
ATAGGAC	25	3.884125E-5	45.000004	1
GGCACGG	25	3.884125E-5	45.000004	2
GCGTTAG	25	3.884125E-5	45.000004	1
TAGGGTA	60	0.0	45.000004	5
AACGAGC	25	3.884125E-5	45.000004	15
ACGTAGG	25	3.884125E-5	45.000004	2
CACAACG	25	3.884125E-5	45.000004	12
CCCGACT	25	3.884125E-5	45.000004	42
CTATGTG	20	7.0252275E-4	45.0	15
TAGCGAG	20	7.0252275E-4	45.0	1
TCGATCT	20	7.0252275E-4	45.0	24
CGTTAGG	40	6.7866495E-9	45.0	2
ATCCGAT	20	7.0252275E-4	45.0	38
TGCGAGG	20	7.0252275E-4	45.0	2
TTGACGG	20	7.0252275E-4	45.0	2
CGTAAGG	20	7.0252275E-4	45.0	2
CGGTAGT	20	7.0252275E-4	45.0	12
CGATCTC	20	7.0252275E-4	45.0	25

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content