##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935172.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1609892
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.129680748770724	33.0	31.0	34.0	30.0	34.0
2	32.26695393231347	34.0	31.0	34.0	30.0	34.0
3	32.18671376713469	33.0	31.0	34.0	30.0	34.0
4	35.89174553324074	37.0	35.0	37.0	35.0	37.0
5	33.25774710353241	37.0	35.0	37.0	30.0	37.0
6	34.375392262338096	37.0	35.0	37.0	28.0	37.0
7	35.699792284202914	37.0	35.0	37.0	32.0	37.0
8	35.73239695582064	37.0	35.0	37.0	35.0	37.0
9	37.8648008686297	39.0	38.0	39.0	35.0	39.0
10	37.41655589319035	39.0	37.0	39.0	34.0	39.0
11	37.28778949146899	39.0	37.0	39.0	34.0	39.0
12	37.49567300166719	39.0	37.0	39.0	35.0	39.0
13	37.45575355365453	39.0	37.0	39.0	35.0	39.0
14	38.533626479291776	40.0	38.0	41.0	34.0	41.0
15	38.682756358811645	40.0	38.0	41.0	34.0	41.0
16	38.68771694001833	40.0	38.0	41.0	34.0	41.0
17	38.6137299893409	40.0	38.0	41.0	34.0	41.0
18	38.29185684505544	39.0	38.0	41.0	34.0	41.0
19	37.849793650754215	39.0	37.0	41.0	34.0	41.0
20	37.41445450999198	39.0	35.0	41.0	34.0	41.0
21	37.29843927418734	39.0	35.0	41.0	33.0	41.0
22	37.26998022227578	39.0	35.0	41.0	33.0	41.0
23	37.07638338472395	38.0	35.0	40.0	33.0	41.0
24	36.80937727499733	38.0	35.0	40.0	33.0	41.0
25	36.5933851463328	38.0	35.0	40.0	33.0	41.0
26	36.84615924546491	38.0	35.0	40.0	33.0	41.0
27	36.768794428446135	38.0	35.0	40.0	33.0	41.0
28	36.7649469653865	38.0	35.0	40.0	33.0	41.0
29	36.73264293505403	38.0	35.0	40.0	33.0	41.0
30	36.37739363882795	38.0	35.0	40.0	32.0	41.0
31	36.04445453483836	38.0	35.0	40.0	31.0	41.0
32	35.5031902761179	38.0	35.0	41.0	26.0	41.0
33	34.93007357015253	38.0	35.0	41.0	21.0	41.0
34	34.39844970966997	38.0	35.0	41.0	17.0	41.0
35	33.98165653348175	38.0	34.0	41.0	15.0	41.0
36	33.73413682408509	38.0	34.0	41.0	10.0	41.0
37	33.653203444703124	38.0	34.0	41.0	10.0	41.0
38	33.5233431807848	38.0	34.0	41.0	10.0	41.0
39	33.38358722199999	38.0	33.0	40.0	10.0	41.0
40	33.24274112797629	38.0	33.0	40.0	10.0	41.0
41	33.08863451709804	38.0	33.0	40.0	10.0	41.0
42	33.0953660245532	38.0	33.0	40.0	10.0	41.0
43	32.99938753655525	38.0	33.0	40.0	10.0	41.0
44	32.90729999279455	38.0	33.0	40.0	10.0	41.0
45	32.890788947333114	38.0	33.0	40.0	10.0	41.0
46	32.82161287837942	38.0	33.0	40.0	10.0	41.0
47	32.757955813184985	37.0	33.0	40.0	10.0	41.0
48	32.68634231364589	37.0	33.0	40.0	10.0	41.0
49	32.64972246585485	37.0	32.0	40.0	10.0	41.0
50	32.55210908557841	37.0	32.0	40.0	9.0	41.0
51	31.800158644182343	36.0	30.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	15.0
9	22.0
10	26.0
11	52.0
12	54.0
13	80.0
14	98.0
15	152.0
16	275.0
17	493.0
18	903.0
19	1644.0
20	2798.0
21	4617.0
22	7439.0
23	12410.0
24	21580.0
25	36793.0
26	52011.0
27	55468.0
28	49386.0
29	41167.0
30	36440.0
31	36219.0
32	41195.0
33	53073.0
34	80068.0
35	112673.0
36	143940.0
37	167273.0
38	283888.0
39	367493.0
40	143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.07375153115861	17.369239675704954	20.25788065286367	33.299128140272764
2	47.604808272853084	18.939220767604287	21.085327462960247	12.37064349658238
3	23.394612806324897	19.131469688649922	45.9579276125355	11.515989892489682
4	20.993085250439158	20.155389305618016	45.40683474419402	13.444690699748804
5	17.22891970393045	28.60291249350888	41.55949591649627	12.608671886064407
6	19.439502774099132	26.44456895245147	43.37533200984911	10.740596263600292
7	61.54872500764026	4.796843514968706	30.605903998529094	3.048527478861936
8	62.57109172540767	4.2305943504284755	30.151525692406693	3.0467882317571613
9	56.94847853147913	7.565165862057828	31.50882170977929	3.977533896683753
10	21.473117451356984	33.968614043674975	35.76370340370658	8.794565101261451
11	20.018734175957146	20.721141542414028	45.436526176911244	13.823598104717583
12	19.267565774598545	18.767470116007782	47.14738628429733	14.817577825096343
13	18.773619596842522	18.726162997269384	48.96527220459509	13.534945201293006
14	14.623838120818041	20.83313663276791	49.488785583132284	15.05423966328176
15	13.283996690461223	22.09359385598537	49.750293808528774	14.872115645024635
16	16.921818358001655	22.569029475269147	46.68021208876124	13.828940077967964
17	16.64620980786289	21.943521677230525	45.7466712052734	15.663597309633193
18	16.344761015024613	21.54144501618742	47.83532063020377	14.278473338584202
19	15.471099924715448	22.75040810190994	46.892648699415865	14.885843273958749
20	15.537191314696885	24.684016070643246	46.38211755819645	13.396675056463414
21	16.104247986821477	22.41237300390337	48.38746947000171	13.095909539273443
22	15.494455528693852	20.350433445224898	48.284791774851975	15.870319251229276
23	13.91161643141279	21.559458646915445	48.84352490726086	15.685400014410906
24	14.318972949738242	22.229752057902022	47.1850285609221	16.266246431437636
25	14.836647427280836	23.65425755268055	45.60734508898733	15.901749931051276
26	14.297853520608836	23.82190855038723	45.61628978838332	16.263948140620613
27	14.388170138121067	22.075890805097483	47.7027030384647	15.833236018316757
28	12.74476797201303	22.34069117679944	47.132043640194496	17.782497210993036
29	14.058085884022034	22.084649156589386	47.27764346925135	16.579621490137228
30	16.18065062749551	21.743073448405234	46.52566756030839	15.550608363790863
31	14.919634360565803	23.450144481741635	45.525289895222784	16.10493126246978
32	15.507686229883744	23.565555950337043	43.73951793039533	17.187239889383886
33	16.99834523061174	22.97911909618782	42.44371672137013	17.578818951830307
34	15.543030215691486	23.846071661949996	42.26190328295314	18.34899483940538
35	15.441097912158083	25.522954334824945	40.709563125973666	18.326384627043304
36	17.743364151135605	25.49494003324447	40.052748880049094	16.708946935570836
37	16.888275735266713	25.82850278155305	39.75869188740611	17.524529595774126
38	17.84343297562818	25.938385928993995	37.83210302305993	18.386078072317893
39	19.51758254590991	24.150688369157685	36.672273668047296	19.65945541688511
40	19.231538513142496	24.779426197533745	37.80266005421481	18.18637523510894
41	17.82827667942943	26.282197812027142	36.80613357914693	19.083391929396505
42	18.602676452830377	25.574448472319883	38.215234313854594	17.607640760995146
43	18.130595095820095	24.59227078586638	38.904845790897774	18.372288327415752
44	18.471425412387912	23.86458222042224	37.83657537275793	19.827416994431925
45	18.254392220099234	23.19354341781933	37.55376137032795	20.998302991753484
46	18.075622464115607	23.97881348562512	38.5924024717186	19.35316157854067
47	16.29146551445687	25.026771982219927	39.9119940964984	18.769768406824806
48	16.870945380186996	24.735013280393964	39.46538028637946	18.928661053039583
49	18.354088348783645	23.399644199735135	39.22536418592055	19.02090326556067
50	16.762801479850822	23.33908113090816	38.90621234219439	20.991905047046632
51	16.833303103562226	23.162485433805497	37.8167603789571	22.187451083675178
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8210.0
1	10888.5
2	13567.0
3	84160.0
4	154753.0
5	95405.0
6	36057.0
7	34174.5
8	32292.0
9	32295.0
10	32298.0
11	31646.0
12	30994.0
13	29601.0
14	28208.0
15	26487.5
16	24767.0
17	23689.5
18	22612.0
19	21313.0
20	20014.0
21	20093.5
22	20173.0
23	19638.0
24	19103.0
25	20130.5
26	23602.5
27	26047.0
28	27619.0
29	29191.0
30	32238.0
31	35285.0
32	37981.5
33	40678.0
34	43544.5
35	46411.0
36	49967.0
37	53523.0
38	54838.0
39	56153.0
40	58256.0
41	60359.0
42	66130.5
43	71902.0
44	76749.5
45	81597.0
46	90879.5
47	100162.0
48	109178.0
49	118194.0
50	116165.5
51	114137.0
52	97684.0
53	81231.0
54	70177.0
55	59123.0
56	51904.0
57	44685.0
58	41354.0
59	38023.0
60	33050.5
61	28078.0
62	24478.5
63	20879.0
64	17683.5
65	14488.0
66	11907.0
67	9326.0
68	7782.0
69	6238.0
70	5215.0
71	4192.0
72	3405.5
73	2619.0
74	2080.5
75	1210.5
76	879.0
77	629.5
78	380.0
79	273.5
80	167.0
81	137.0
82	107.0
83	80.0
84	53.0
85	38.0
86	23.0
87	15.0
88	7.0
89	4.0
90	1.0
91	1.5
92	2.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1609892.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.12094789388475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.4025901631523	24.97405259857714
2	8.914381013084025	5.90505498081183
3	3.640177505794425	3.616983884817255
4	2.0629307359426163	2.7330488565539484
5	1.3673670632826942	2.264424662740016
6	0.9856178200035433	1.9586757875772978
7	0.7900692220454775	1.831748907515122
8	0.6396324273085452	1.694818583690028
9	0.551343076763535	1.6434904785425228
>10	5.334318356835767	34.258676229567364
>50	0.23648705132993772	5.177048190940746
>100	0.06869942695382306	3.751700395582126
>500	0.002128712501116262	0.48821490991015287
>1k	0.0034833477290993372	1.8420386367215287
>5k	5.805579548498895E-4	1.2219794220082796
>10k+	1.9351931828329652E-4	6.63804347444466
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	103565	6.433040228785533	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7224	0.44872575303188045	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCC	6400	0.39754219537708124	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5441	0.3379729820385467	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC	2356	0.146345220673188	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCG	2336	0.14510290131263462	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2217	0.13771110111734203	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2106	0.13081622866627077	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1870	0.1161568602117409	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1823	0.11323740971444048	No Hit
GCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC	1682	0.10447905822253914	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGT	1612	0.10013094046060234	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2423193605533788E-4	0.0	0.0	0.0949753151143058	0.0
2	1.2423193605533788E-4	0.0	0.0	0.4553100456428133	0.0
3	1.2423193605533788E-4	0.0	0.0	0.7239616073624815	0.0
4	1.2423193605533788E-4	0.0	0.0	1.0575864716390913	0.0
5	1.2423193605533788E-4	0.0	0.0	1.9426147840973182	0.0
6	1.2423193605533788E-4	0.0	0.0	2.660302678689005	0.0
7	1.2423193605533788E-4	0.0	0.0	3.3117128353951695	0.0
8	1.2423193605533788E-4	0.0	0.0	4.250533576165358	0.0
9	1.2423193605533788E-4	0.0	0.0	4.685904396071289	0.0
10	1.2423193605533788E-4	0.0	0.0	5.689201511654198	0.0
11	1.2423193605533788E-4	0.0	0.0	7.346828234440571	0.0
12	1.2423193605533788E-4	0.0	0.0	8.47851905593667	0.0
13	1.2423193605533788E-4	0.0	0.0	8.920225704581425	0.0
14	1.2423193605533788E-4	0.0	0.0	9.13409098250069	0.0
15	1.2423193605533788E-4	0.0	0.0	9.454609377523461	0.0
16	1.2423193605533788E-4	0.0	0.0	10.05446328076666	0.0
17	1.2423193605533788E-4	0.0	0.0	10.80911017633481	0.0
18	1.2423193605533788E-4	0.0	0.0	11.561396665117908	0.0
19	1.2423193605533788E-4	0.0	0.0	12.076089576195173	0.0
20	1.2423193605533788E-4	0.0	0.0	12.685012410770412	0.0
21	1.8634790408300682E-4	0.0	0.0	13.33070789841803	0.0
22	1.8634790408300682E-4	0.0	0.0	14.009449081056369	0.0
23	1.8634790408300682E-4	0.0	0.0	14.580791754974868	0.0
24	1.8634790408300682E-4	0.0	0.0	14.981377632785305	0.0
25	1.8634790408300682E-4	0.0	0.0	15.337612709423986	0.0
26	1.8634790408300682E-4	0.0	0.0	15.646453302457557	0.0
27	1.8634790408300682E-4	0.0	0.0	15.976413324620534	0.0
28	1.8634790408300682E-4	0.0	0.0	16.27811058133092	0.0
29	1.8634790408300682E-4	0.0	0.0	16.612729301095975	0.0
30	1.8634790408300682E-4	0.0	0.0	16.972256524040123	0.0
31	1.8634790408300682E-4	0.0	0.0	17.27128279412532	0.0
32	1.8634790408300682E-4	0.0	0.0	17.55484218817163	0.0
33	1.8634790408300682E-4	0.0	0.0	17.81734426905656	0.0
34	1.8634790408300682E-4	0.0	0.0	18.088107773689167	0.0
35	1.8634790408300682E-4	0.0	0.0	18.36166649688302	0.0
36	1.8634790408300682E-4	0.0	0.0	18.617025241444768	0.0
37	2.4846387211067576E-4	0.0	0.0	18.874061117143263	0.0
38	2.4846387211067576E-4	0.0	0.0	19.18029283951967	0.0
39	3.105798401383447E-4	0.0	0.0	19.713869004877346	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGAC	20	7.0341286E-4	45.000004	25
CCGAATA	20	7.0341286E-4	45.000004	42
TATCGCG	20	7.0341286E-4	45.000004	1
TAGTACG	45	3.8562575E-10	45.0	1
CGTTTTT	35050	0.0	44.22325	1
CGTTATT	2640	0.0	43.721592	1
CGACGGT	240	0.0	43.124996	28
CGACAGG	70	0.0	41.785713	2
CGGTCTA	255	0.0	40.588234	31
ACGGGTA	145	0.0	40.344826	5
CGAATAT	110	0.0	38.863636	14
ACAACGA	470	0.0	38.776596	13
CACGACG	280	0.0	38.571426	26
GTTTTTT	41980	0.0	37.98952	2
CGTAAGG	160	0.0	37.968754	2
AGCGACT	505	0.0	37.87129	19
CTCACGA	280	0.0	37.767857	24
CGCGACG	30	1.14012524E-4	37.499996	1
CCGTTTA	30	1.14012524E-4	37.499996	26
TCGGTAG	30	1.14012524E-4	37.499996	1
>>END_MODULE