SRR2935171.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935171.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	482155
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13721	2.845765365909303	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4787	0.9928342545447003	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCC	4422	0.9171324574047764	No Hit
CCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	3256	0.6753015109249101	No Hit
GCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	3157	0.6547686947143553	No Hit
CTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGCT	2123	0.4403148365152285	No Hit
TCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	1795	0.3722869201812695	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1680	0.3484356690275948	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCG	1379	0.2860076116601508	No Hit
ACTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	1148	0.2380977071688565	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1039	0.21549086911885185	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGC	1018	0.2111354232560069	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	953	0.19765428129958207	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	863	0.17898808474453234	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	843	0.17484004106563242	No Hit
ACCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTG	671	0.13916686542709295	No Hit
AGCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTG	667	0.13833725669131297	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGT	634	0.13149298462112805	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTC	586	0.1215376797917682	No Hit
CGCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTG	565	0.11718223392892327	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	508	0.10536030944405844	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGTC	35	1.2099736E-7	45.000004	32
CTAACGG	35	1.2099736E-7	45.000004	2
ACGTCTC	35	1.2099736E-7	45.000004	34
CGCACGG	35	1.2099736E-7	45.000004	2
ATACGAG	35	1.2099736E-7	45.000004	1
TCACGAC	25	3.8874252E-5	45.0	25
TTGATCG	20	7.0292095E-4	45.0	1
AATCCGG	20	7.0292095E-4	45.0	2
CTCACGA	25	3.8874252E-5	45.0	24
GTTTCAT	20	7.0292095E-4	45.0	10
CGACGGT	25	3.8874252E-5	45.0	28
GCGCGAG	20	7.0292095E-4	45.0	1
CGTTGTT	25	3.8874252E-5	45.0	1
GTGGGCG	20	7.0292095E-4	45.0	5
ATTACGG	25	3.8874252E-5	45.0	2
GGCAACG	20	7.0292095E-4	45.0	1
ATAGCGG	25	3.8874252E-5	45.0	2
CGTCTGG	25	3.8874252E-5	45.0	2
TTACTGA	20	7.0292095E-4	45.0	7
GTTAGCG	25	3.8874252E-5	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content