##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935171.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 482155 Sequences flagged as poor quality 0 Sequence length 51 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.58201823065197 31.0 31.0 34.0 30.0 34.0 2 31.686752185500513 31.0 31.0 34.0 30.0 34.0 3 31.68721884041439 31.0 31.0 34.0 30.0 34.0 4 35.51787080917962 37.0 35.0 37.0 33.0 37.0 5 32.95795335524883 37.0 35.0 37.0 28.0 37.0 6 33.99431303211623 37.0 35.0 37.0 27.0 37.0 7 35.339575447729466 37.0 35.0 37.0 32.0 37.0 8 35.34584314172828 37.0 35.0 37.0 33.0 37.0 9 37.4002260683805 39.0 37.0 39.0 34.0 39.0 10 36.802781263286704 39.0 37.0 39.0 32.0 39.0 11 36.6023394966349 39.0 37.0 39.0 32.0 39.0 12 37.034447428731426 39.0 37.0 39.0 33.0 39.0 13 37.03231948232415 39.0 37.0 39.0 33.0 39.0 14 37.96313633582562 40.0 37.0 41.0 33.0 41.0 15 38.08401032862876 40.0 37.0 41.0 33.0 41.0 16 38.079152969480766 40.0 37.0 41.0 33.0 41.0 17 38.03135091412513 40.0 37.0 41.0 33.0 41.0 18 37.93276021196503 39.0 37.0 40.0 33.0 41.0 19 37.69645031162178 39.0 37.0 40.0 33.0 41.0 20 37.49563314701704 39.0 35.0 40.0 33.0 41.0 21 37.42202196389128 39.0 35.0 40.0 33.0 41.0 22 37.375912310356625 39.0 35.0 40.0 33.0 41.0 23 37.11717186381973 39.0 35.0 40.0 32.0 41.0 24 36.79247959681015 39.0 35.0 40.0 32.0 41.0 25 36.577158797482134 38.0 35.0 40.0 31.0 41.0 26 36.87381858530971 38.0 35.0 40.0 32.0 41.0 27 36.73946345055013 38.0 35.0 40.0 32.0 41.0 28 36.82454812249173 38.0 35.0 40.0 32.0 41.0 29 36.84771702046022 39.0 35.0 40.0 32.0 41.0 30 36.51489666186185 38.0 35.0 40.0 31.0 41.0 31 36.354813286183905 38.0 35.0 40.0 31.0 41.0 32 36.046698675737055 38.0 35.0 40.0 30.0 41.0 33 35.881500762203025 38.0 35.0 40.0 30.0 41.0 34 35.76063506548724 38.0 35.0 40.0 29.0 41.0 35 35.49213634619573 38.0 35.0 40.0 27.0 41.0 36 35.37194263255592 38.0 35.0 40.0 25.0 41.0 37 35.3010359739088 38.0 35.0 40.0 25.0 41.0 38 35.170915991745396 38.0 35.0 40.0 25.0 41.0 39 35.187051881656316 38.0 35.0 40.0 24.0 41.0 40 35.10279474442866 38.0 35.0 40.0 24.0 41.0 41 34.98787319430473 38.0 35.0 40.0 24.0 41.0 42 35.0883761446008 38.0 35.0 40.0 24.0 41.0 43 35.015640198691294 38.0 35.0 40.0 23.0 41.0 44 34.94371312129917 38.0 35.0 40.0 23.0 41.0 45 34.93164853625909 38.0 35.0 40.0 23.0 41.0 46 34.79591832501996 38.0 35.0 40.0 23.0 41.0 47 34.6791861538302 38.0 35.0 40.0 22.0 41.0 48 34.67093776897471 38.0 35.0 40.0 22.0 41.0 49 34.699335276000454 38.0 35.0 40.0 22.0 41.0 50 34.63262228951271 38.0 35.0 40.0 21.0 41.0 51 33.80834793790379 37.0 33.0 40.0 20.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 3.0 9 4.0 10 13.0 11 10.0 12 11.0 13 28.0 14 24.0 15 45.0 16 52.0 17 145.0 18 214.0 19 429.0 20 668.0 21 1116.0 22 1757.0 23 2741.0 24 4137.0 25 6158.0 26 7893.0 27 8470.0 28 8489.0 29 8436.0 30 9180.0 31 10985.0 32 13974.0 33 18900.0 34 28191.0 35 44214.0 36 60155.0 37 72025.0 38 101412.0 39 72259.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.91172963051301 24.18454646327426 24.956912196285426 18.946811709927307 2 36.88129336001908 24.185376072010037 24.306084143066027 14.627246424904856 3 25.223838807022638 23.69072186330122 37.624415385094004 13.461023944582138 4 23.987721790710456 25.666642469745206 35.46784747643393 14.87778826311041 5 17.857328037664235 33.13996536383528 33.61657558254089 15.386131015959597 6 20.859267248084123 31.88642656407172 34.559633312938786 12.694672874905372 7 65.20226897989237 7.552550528357063 21.729319409733385 5.515861082017193 8 63.10356628055294 6.5319243811637335 22.36625151662847 7.998257821654862 9 58.112847528284476 9.588410365961153 24.843878006035403 7.454864099718971 10 26.646202984517426 31.069261959328433 30.23903101699661 12.045504039157533 11 16.282108450601985 26.313115077101763 37.2745279007788 20.13024857151746 12 16.944965830490194 22.127324200723834 43.32569401955803 17.602015949227944 13 18.817185344961683 24.945090271800563 42.373717995250495 13.864006387987265 14 14.456554427518123 28.068981966380107 39.6123653182068 17.86209828789497 15 11.579471331833124 29.109103918864264 42.1018137320986 17.20961101720401 16 12.275513061152534 30.232601549294312 38.14126162748494 19.350623762068214 17 11.996764525930457 30.91267331044996 35.56574130725597 21.524820856363615 18 12.1504495442337 29.310906243842748 41.13718617457042 17.401458037353134 19 12.365110804616773 31.20863622693947 37.99400607688399 18.432246891559767 20 12.441642210492477 33.760305296014764 37.81999564455414 15.978056848938618 21 13.389470191121111 31.74705229646068 38.85908058611857 16.004396926299634 22 10.873059493316465 31.233524489012872 34.82801173896361 23.065404278707057 23 11.050595762773382 30.10235297777686 36.42127531602908 22.425775943420685 24 14.572492248343375 29.107652103576655 35.232445997656356 21.08740965042362 25 11.452955999626676 31.92583297902127 32.85250593688752 23.76870508446454 26 10.744055334902676 32.34146695564704 35.11941180740633 21.795065902043948 27 14.408229718658937 32.03886716927129 34.71207391813836 18.840829193931413 28 11.740830230942331 30.44415177691821 34.40055583785298 23.414462154286483 29 13.57198411299271 29.448413891798282 32.89336416712468 24.086237828084332 30 18.682788729765324 32.923437483796704 29.503790274911594 18.889983511526374 31 16.116186703445987 35.52094243552384 25.997863757505367 22.3650071035248 32 14.026194895832253 37.91021559457021 25.399093652456163 22.664495857141375 33 18.25554023083863 32.76664143273429 27.543839636631372 21.43397869979571 34 15.633354419222034 30.039717518225466 27.599216019744688 26.72771204280781 35 13.467660814468378 34.51089379971171 25.2066244257552 26.81482096006471 36 18.310709211768 33.82480737522166 27.462123176157043 20.402360236853294 37 17.52424013024857 34.73862139768332 28.886976179859175 18.850162292208935 38 17.759434206842197 37.44086445230268 26.233057834098993 18.566643506756126 39 21.24316869056631 28.74739451006419 29.8476630958924 20.1617737034771 40 19.13388847984569 28.380292644481546 28.936130497454137 23.549688378218622 41 15.229957171449016 32.98607294334809 26.61986290715641 25.16410697804648 42 16.974416940610386 30.61297715464944 32.016467733405236 20.396138171334943 43 19.340668457238856 29.504619883647376 31.77442938474142 19.380282274372348 44 19.258122388028745 28.342960251371448 27.493233503748794 24.905683856851013 45 21.54493886820628 25.92651740622829 26.3736765148137 26.154867210751732 46 21.8469164480302 28.39232197115036 28.570273044975163 21.19048853584428 47 14.92756478725721 30.580829816137967 32.9155562008068 21.57604919579803 48 16.02244091630285 28.71835820431189 32.515477387976894 22.743723491408367 49 20.113448994617915 24.913357737656977 34.73986581078699 20.23332745693812 50 18.281880308199646 23.41301033899887 32.22698095010941 26.07812840269208 51 16.836287086103017 22.986176644440064 28.368885524364572 31.808650745092347 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5928.0 1 6572.5 2 7217.0 3 13930.0 4 20643.0 5 12972.5 6 5302.0 7 5572.5 8 5843.0 9 6854.5 10 7866.0 11 9141.0 12 10416.0 13 10702.5 14 10989.0 15 9888.5 16 8788.0 17 7534.5 18 6281.0 19 5582.0 20 4883.0 21 4627.5 22 4372.0 23 4297.5 24 4223.0 25 4168.0 26 4636.5 27 5160.0 28 5078.5 29 4997.0 30 5606.0 31 6215.0 32 6367.5 33 6520.0 34 6960.0 35 7400.0 36 7664.5 37 7929.0 38 8915.5 39 9902.0 40 11751.5 41 13601.0 42 18277.5 43 22954.0 44 27246.5 45 31539.0 46 41360.0 47 51181.0 48 61660.0 49 72139.0 50 68036.5 51 63934.0 52 48392.0 53 32850.0 54 24595.0 55 16340.0 56 12155.0 57 7970.0 58 6458.5 59 4947.0 60 4250.0 61 3553.0 62 3073.5 63 2594.0 64 2072.5 65 1551.0 66 1239.0 67 927.0 68 684.5 69 442.0 70 375.5 71 309.0 72 235.5 73 162.0 74 104.5 75 40.0 76 33.0 77 39.5 78 46.0 79 40.5 80 35.0 81 21.5 82 8.0 83 6.0 84 4.0 85 2.0 86 0.0 87 0.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 482155.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.2730697131053 #Duplication Level Percentage of deduplicated Percentage of total 1 73.53769081492591 30.35126239545221 2 11.16482875787389 9.216135113172239 3 4.62461152721396 5.726157418761965 4 2.561588681446659 4.228985129025978 5 1.6568115514305277 3.419084933183516 6 1.1702794731623518 2.8980615767787534 7 0.8518313277348869 2.461038564138633 8 0.7086830058123256 2.33996184827081 9 0.57717464771386 2.1439592524558027 >10 2.9655368255948633 19.971208148919516 >50 0.11520486051411975 3.200903241261933 >100 0.05505365015717139 4.404134846149501 >500 0.0045878041797642825 1.3233654126155046 >1k 0.005607316219711901 5.428954071085927 >5k 0.0 0.0 >10k+ 5.097560199738092E-4 2.8867880487277167 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13721 2.845765365909303 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4787 0.9928342545447003 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCC 4422 0.9171324574047764 No Hit CCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC 3256 0.6753015109249101 No Hit GCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC 3157 0.6547686947143553 No Hit CTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGCT 2123 0.4403148365152285 No Hit TCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC 1795 0.3722869201812695 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1680 0.3484356690275948 No Hit CGTTTTTTTCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCG 1379 0.2860076116601508 No Hit ACTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC 1148 0.2380977071688565 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1039 0.21549086911885185 No Hit CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGC 1018 0.2111354232560069 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 953 0.19765428129958207 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 863 0.17898808474453234 No Hit TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 843 0.17484004106563242 No Hit ACCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTG 671 0.13916686542709295 No Hit AGCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTG 667 0.13833725669131297 No Hit CGTTTTTTCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGT 634 0.13149298462112805 No Hit CGTTTTTCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTC 586 0.1215376797917682 No Hit CGCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTG 565 0.11718223392892327 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 508 0.10536030944405844 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.0740218394499693E-4 0.0 0.0 0.5062687310097376 0.0 2 2.0740218394499693E-4 0.0 0.0 2.69996163059597 0.0 3 2.0740218394499693E-4 0.0 0.0 3.7062770270970953 0.0 4 2.0740218394499693E-4 0.0 0.0 5.036347232736361 0.0 5 2.0740218394499693E-4 0.0 0.0 8.44686874552789 0.0 6 2.0740218394499693E-4 0.0 0.0 10.290881562982857 0.0 7 2.0740218394499693E-4 0.0 0.0 12.404309817382376 0.0 8 2.0740218394499693E-4 0.0 0.0 15.53483838184816 0.0 9 2.0740218394499693E-4 0.0 0.0 16.616233368937376 0.0 10 2.0740218394499693E-4 0.0 0.0 19.57378851199303 0.0 11 2.0740218394499693E-4 0.0 0.0 24.902987628459726 0.0 12 2.0740218394499693E-4 0.0 0.0 28.432143190467794 0.0 13 2.0740218394499693E-4 0.0 0.0 29.72156256805384 0.0 14 2.0740218394499693E-4 0.0 0.0 30.17639555744522 0.0 15 2.0740218394499693E-4 0.0 0.0 31.053499393348613 0.0 16 2.0740218394499693E-4 0.0 0.0 32.942933289087534 0.0 17 2.0740218394499693E-4 0.0 0.0 35.448144268959155 0.0 18 2.0740218394499693E-4 0.0 0.0 38.00997604504776 0.0 19 2.0740218394499693E-4 0.0 0.0 39.68848191971461 0.0 20 2.0740218394499693E-4 0.0 0.0 41.2477315386131 0.0 21 2.0740218394499693E-4 0.0 0.0 43.054204560774025 0.0 22 2.0740218394499693E-4 0.0 0.0 45.01581441652581 0.0 23 2.0740218394499693E-4 0.0 0.0 46.79179931764681 0.0 24 2.0740218394499693E-4 0.0 0.0 47.88709025106034 0.0 25 2.0740218394499693E-4 0.0 0.0 48.7303875309807 0.0 26 2.0740218394499693E-4 0.0 0.0 49.42705146685194 0.0 27 2.0740218394499693E-4 0.0 0.0 50.04614698592776 0.0 28 2.0740218394499693E-4 0.0 0.0 50.672086777073766 0.0 29 2.0740218394499693E-4 0.0 0.0 51.31192251454408 0.0 30 2.0740218394499693E-4 0.0 0.0 51.928321805228606 0.0 31 2.0740218394499693E-4 0.0 0.0 52.47918200578652 0.0 32 4.1480436788999387E-4 0.0 0.0 52.94500731092698 0.0 33 4.1480436788999387E-4 0.0 0.0 53.36935217927845 0.0 34 4.1480436788999387E-4 0.0 0.0 53.758853480727154 0.0 35 4.1480436788999387E-4 0.0 0.0 54.153539836774485 0.0 36 4.1480436788999387E-4 0.0 0.0 54.52043430017318 0.0 37 4.1480436788999387E-4 0.0 0.0 54.898943285872804 0.0 38 4.1480436788999387E-4 0.0 0.0 55.24240130248572 0.0 39 4.1480436788999387E-4 0.0 0.0 55.58378529725918 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGTC 35 1.2099736E-7 45.000004 32 CTAACGG 35 1.2099736E-7 45.000004 2 ACGTCTC 35 1.2099736E-7 45.000004 34 CGCACGG 35 1.2099736E-7 45.000004 2 ATACGAG 35 1.2099736E-7 45.000004 1 TCACGAC 25 3.8874252E-5 45.0 25 TTGATCG 20 7.0292095E-4 45.0 1 AATCCGG 20 7.0292095E-4 45.0 2 CTCACGA 25 3.8874252E-5 45.0 24 GTTTCAT 20 7.0292095E-4 45.0 10 CGACGGT 25 3.8874252E-5 45.0 28 GCGCGAG 20 7.0292095E-4 45.0 1 CGTTGTT 25 3.8874252E-5 45.0 1 GTGGGCG 20 7.0292095E-4 45.0 5 ATTACGG 25 3.8874252E-5 45.0 2 GGCAACG 20 7.0292095E-4 45.0 1 ATAGCGG 25 3.8874252E-5 45.0 2 CGTCTGG 25 3.8874252E-5 45.0 2 TTACTGA 20 7.0292095E-4 45.0 7 GTTAGCG 25 3.8874252E-5 45.0 1 >>END_MODULE