##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935171.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	482155
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58201823065197	31.0	31.0	34.0	30.0	34.0
2	31.686752185500513	31.0	31.0	34.0	30.0	34.0
3	31.68721884041439	31.0	31.0	34.0	30.0	34.0
4	35.51787080917962	37.0	35.0	37.0	33.0	37.0
5	32.95795335524883	37.0	35.0	37.0	28.0	37.0
6	33.99431303211623	37.0	35.0	37.0	27.0	37.0
7	35.339575447729466	37.0	35.0	37.0	32.0	37.0
8	35.34584314172828	37.0	35.0	37.0	33.0	37.0
9	37.4002260683805	39.0	37.0	39.0	34.0	39.0
10	36.802781263286704	39.0	37.0	39.0	32.0	39.0
11	36.6023394966349	39.0	37.0	39.0	32.0	39.0
12	37.034447428731426	39.0	37.0	39.0	33.0	39.0
13	37.03231948232415	39.0	37.0	39.0	33.0	39.0
14	37.96313633582562	40.0	37.0	41.0	33.0	41.0
15	38.08401032862876	40.0	37.0	41.0	33.0	41.0
16	38.079152969480766	40.0	37.0	41.0	33.0	41.0
17	38.03135091412513	40.0	37.0	41.0	33.0	41.0
18	37.93276021196503	39.0	37.0	40.0	33.0	41.0
19	37.69645031162178	39.0	37.0	40.0	33.0	41.0
20	37.49563314701704	39.0	35.0	40.0	33.0	41.0
21	37.42202196389128	39.0	35.0	40.0	33.0	41.0
22	37.375912310356625	39.0	35.0	40.0	33.0	41.0
23	37.11717186381973	39.0	35.0	40.0	32.0	41.0
24	36.79247959681015	39.0	35.0	40.0	32.0	41.0
25	36.577158797482134	38.0	35.0	40.0	31.0	41.0
26	36.87381858530971	38.0	35.0	40.0	32.0	41.0
27	36.73946345055013	38.0	35.0	40.0	32.0	41.0
28	36.82454812249173	38.0	35.0	40.0	32.0	41.0
29	36.84771702046022	39.0	35.0	40.0	32.0	41.0
30	36.51489666186185	38.0	35.0	40.0	31.0	41.0
31	36.354813286183905	38.0	35.0	40.0	31.0	41.0
32	36.046698675737055	38.0	35.0	40.0	30.0	41.0
33	35.881500762203025	38.0	35.0	40.0	30.0	41.0
34	35.76063506548724	38.0	35.0	40.0	29.0	41.0
35	35.49213634619573	38.0	35.0	40.0	27.0	41.0
36	35.37194263255592	38.0	35.0	40.0	25.0	41.0
37	35.3010359739088	38.0	35.0	40.0	25.0	41.0
38	35.170915991745396	38.0	35.0	40.0	25.0	41.0
39	35.187051881656316	38.0	35.0	40.0	24.0	41.0
40	35.10279474442866	38.0	35.0	40.0	24.0	41.0
41	34.98787319430473	38.0	35.0	40.0	24.0	41.0
42	35.0883761446008	38.0	35.0	40.0	24.0	41.0
43	35.015640198691294	38.0	35.0	40.0	23.0	41.0
44	34.94371312129917	38.0	35.0	40.0	23.0	41.0
45	34.93164853625909	38.0	35.0	40.0	23.0	41.0
46	34.79591832501996	38.0	35.0	40.0	23.0	41.0
47	34.6791861538302	38.0	35.0	40.0	22.0	41.0
48	34.67093776897471	38.0	35.0	40.0	22.0	41.0
49	34.699335276000454	38.0	35.0	40.0	22.0	41.0
50	34.63262228951271	38.0	35.0	40.0	21.0	41.0
51	33.80834793790379	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	4.0
10	13.0
11	10.0
12	11.0
13	28.0
14	24.0
15	45.0
16	52.0
17	145.0
18	214.0
19	429.0
20	668.0
21	1116.0
22	1757.0
23	2741.0
24	4137.0
25	6158.0
26	7893.0
27	8470.0
28	8489.0
29	8436.0
30	9180.0
31	10985.0
32	13974.0
33	18900.0
34	28191.0
35	44214.0
36	60155.0
37	72025.0
38	101412.0
39	72259.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.91172963051301	24.18454646327426	24.956912196285426	18.946811709927307
2	36.88129336001908	24.185376072010037	24.306084143066027	14.627246424904856
3	25.223838807022638	23.69072186330122	37.624415385094004	13.461023944582138
4	23.987721790710456	25.666642469745206	35.46784747643393	14.87778826311041
5	17.857328037664235	33.13996536383528	33.61657558254089	15.386131015959597
6	20.859267248084123	31.88642656407172	34.559633312938786	12.694672874905372
7	65.20226897989237	7.552550528357063	21.729319409733385	5.515861082017193
8	63.10356628055294	6.5319243811637335	22.36625151662847	7.998257821654862
9	58.112847528284476	9.588410365961153	24.843878006035403	7.454864099718971
10	26.646202984517426	31.069261959328433	30.23903101699661	12.045504039157533
11	16.282108450601985	26.313115077101763	37.2745279007788	20.13024857151746
12	16.944965830490194	22.127324200723834	43.32569401955803	17.602015949227944
13	18.817185344961683	24.945090271800563	42.373717995250495	13.864006387987265
14	14.456554427518123	28.068981966380107	39.6123653182068	17.86209828789497
15	11.579471331833124	29.109103918864264	42.1018137320986	17.20961101720401
16	12.275513061152534	30.232601549294312	38.14126162748494	19.350623762068214
17	11.996764525930457	30.91267331044996	35.56574130725597	21.524820856363615
18	12.1504495442337	29.310906243842748	41.13718617457042	17.401458037353134
19	12.365110804616773	31.20863622693947	37.99400607688399	18.432246891559767
20	12.441642210492477	33.760305296014764	37.81999564455414	15.978056848938618
21	13.389470191121111	31.74705229646068	38.85908058611857	16.004396926299634
22	10.873059493316465	31.233524489012872	34.82801173896361	23.065404278707057
23	11.050595762773382	30.10235297777686	36.42127531602908	22.425775943420685
24	14.572492248343375	29.107652103576655	35.232445997656356	21.08740965042362
25	11.452955999626676	31.92583297902127	32.85250593688752	23.76870508446454
26	10.744055334902676	32.34146695564704	35.11941180740633	21.795065902043948
27	14.408229718658937	32.03886716927129	34.71207391813836	18.840829193931413
28	11.740830230942331	30.44415177691821	34.40055583785298	23.414462154286483
29	13.57198411299271	29.448413891798282	32.89336416712468	24.086237828084332
30	18.682788729765324	32.923437483796704	29.503790274911594	18.889983511526374
31	16.116186703445987	35.52094243552384	25.997863757505367	22.3650071035248
32	14.026194895832253	37.91021559457021	25.399093652456163	22.664495857141375
33	18.25554023083863	32.76664143273429	27.543839636631372	21.43397869979571
34	15.633354419222034	30.039717518225466	27.599216019744688	26.72771204280781
35	13.467660814468378	34.51089379971171	25.2066244257552	26.81482096006471
36	18.310709211768	33.82480737522166	27.462123176157043	20.402360236853294
37	17.52424013024857	34.73862139768332	28.886976179859175	18.850162292208935
38	17.759434206842197	37.44086445230268	26.233057834098993	18.566643506756126
39	21.24316869056631	28.74739451006419	29.8476630958924	20.1617737034771
40	19.13388847984569	28.380292644481546	28.936130497454137	23.549688378218622
41	15.229957171449016	32.98607294334809	26.61986290715641	25.16410697804648
42	16.974416940610386	30.61297715464944	32.016467733405236	20.396138171334943
43	19.340668457238856	29.504619883647376	31.77442938474142	19.380282274372348
44	19.258122388028745	28.342960251371448	27.493233503748794	24.905683856851013
45	21.54493886820628	25.92651740622829	26.3736765148137	26.154867210751732
46	21.8469164480302	28.39232197115036	28.570273044975163	21.19048853584428
47	14.92756478725721	30.580829816137967	32.9155562008068	21.57604919579803
48	16.02244091630285	28.71835820431189	32.515477387976894	22.743723491408367
49	20.113448994617915	24.913357737656977	34.73986581078699	20.23332745693812
50	18.281880308199646	23.41301033899887	32.22698095010941	26.07812840269208
51	16.836287086103017	22.986176644440064	28.368885524364572	31.808650745092347
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5928.0
1	6572.5
2	7217.0
3	13930.0
4	20643.0
5	12972.5
6	5302.0
7	5572.5
8	5843.0
9	6854.5
10	7866.0
11	9141.0
12	10416.0
13	10702.5
14	10989.0
15	9888.5
16	8788.0
17	7534.5
18	6281.0
19	5582.0
20	4883.0
21	4627.5
22	4372.0
23	4297.5
24	4223.0
25	4168.0
26	4636.5
27	5160.0
28	5078.5
29	4997.0
30	5606.0
31	6215.0
32	6367.5
33	6520.0
34	6960.0
35	7400.0
36	7664.5
37	7929.0
38	8915.5
39	9902.0
40	11751.5
41	13601.0
42	18277.5
43	22954.0
44	27246.5
45	31539.0
46	41360.0
47	51181.0
48	61660.0
49	72139.0
50	68036.5
51	63934.0
52	48392.0
53	32850.0
54	24595.0
55	16340.0
56	12155.0
57	7970.0
58	6458.5
59	4947.0
60	4250.0
61	3553.0
62	3073.5
63	2594.0
64	2072.5
65	1551.0
66	1239.0
67	927.0
68	684.5
69	442.0
70	375.5
71	309.0
72	235.5
73	162.0
74	104.5
75	40.0
76	33.0
77	39.5
78	46.0
79	40.5
80	35.0
81	21.5
82	8.0
83	6.0
84	4.0
85	2.0
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	482155.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.2730697131053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.53769081492591	30.35126239545221
2	11.16482875787389	9.216135113172239
3	4.62461152721396	5.726157418761965
4	2.561588681446659	4.228985129025978
5	1.6568115514305277	3.419084933183516
6	1.1702794731623518	2.8980615767787534
7	0.8518313277348869	2.461038564138633
8	0.7086830058123256	2.33996184827081
9	0.57717464771386	2.1439592524558027
>10	2.9655368255948633	19.971208148919516
>50	0.11520486051411975	3.200903241261933
>100	0.05505365015717139	4.404134846149501
>500	0.0045878041797642825	1.3233654126155046
>1k	0.005607316219711901	5.428954071085927
>5k	0.0	0.0
>10k+	5.097560199738092E-4	2.8867880487277167
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13721	2.845765365909303	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4787	0.9928342545447003	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCC	4422	0.9171324574047764	No Hit
CCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	3256	0.6753015109249101	No Hit
GCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	3157	0.6547686947143553	No Hit
CTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGCT	2123	0.4403148365152285	No Hit
TCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	1795	0.3722869201812695	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1680	0.3484356690275948	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCG	1379	0.2860076116601508	No Hit
ACTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	1148	0.2380977071688565	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1039	0.21549086911885185	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGC	1018	0.2111354232560069	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	953	0.19765428129958207	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	863	0.17898808474453234	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	843	0.17484004106563242	No Hit
ACCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTG	671	0.13916686542709295	No Hit
AGCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTG	667	0.13833725669131297	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGT	634	0.13149298462112805	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTC	586	0.1215376797917682	No Hit
CGCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTG	565	0.11718223392892327	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	508	0.10536030944405844	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0740218394499693E-4	0.0	0.0	0.5062687310097376	0.0
2	2.0740218394499693E-4	0.0	0.0	2.69996163059597	0.0
3	2.0740218394499693E-4	0.0	0.0	3.7062770270970953	0.0
4	2.0740218394499693E-4	0.0	0.0	5.036347232736361	0.0
5	2.0740218394499693E-4	0.0	0.0	8.44686874552789	0.0
6	2.0740218394499693E-4	0.0	0.0	10.290881562982857	0.0
7	2.0740218394499693E-4	0.0	0.0	12.404309817382376	0.0
8	2.0740218394499693E-4	0.0	0.0	15.53483838184816	0.0
9	2.0740218394499693E-4	0.0	0.0	16.616233368937376	0.0
10	2.0740218394499693E-4	0.0	0.0	19.57378851199303	0.0
11	2.0740218394499693E-4	0.0	0.0	24.902987628459726	0.0
12	2.0740218394499693E-4	0.0	0.0	28.432143190467794	0.0
13	2.0740218394499693E-4	0.0	0.0	29.72156256805384	0.0
14	2.0740218394499693E-4	0.0	0.0	30.17639555744522	0.0
15	2.0740218394499693E-4	0.0	0.0	31.053499393348613	0.0
16	2.0740218394499693E-4	0.0	0.0	32.942933289087534	0.0
17	2.0740218394499693E-4	0.0	0.0	35.448144268959155	0.0
18	2.0740218394499693E-4	0.0	0.0	38.00997604504776	0.0
19	2.0740218394499693E-4	0.0	0.0	39.68848191971461	0.0
20	2.0740218394499693E-4	0.0	0.0	41.2477315386131	0.0
21	2.0740218394499693E-4	0.0	0.0	43.054204560774025	0.0
22	2.0740218394499693E-4	0.0	0.0	45.01581441652581	0.0
23	2.0740218394499693E-4	0.0	0.0	46.79179931764681	0.0
24	2.0740218394499693E-4	0.0	0.0	47.88709025106034	0.0
25	2.0740218394499693E-4	0.0	0.0	48.7303875309807	0.0
26	2.0740218394499693E-4	0.0	0.0	49.42705146685194	0.0
27	2.0740218394499693E-4	0.0	0.0	50.04614698592776	0.0
28	2.0740218394499693E-4	0.0	0.0	50.672086777073766	0.0
29	2.0740218394499693E-4	0.0	0.0	51.31192251454408	0.0
30	2.0740218394499693E-4	0.0	0.0	51.928321805228606	0.0
31	2.0740218394499693E-4	0.0	0.0	52.47918200578652	0.0
32	4.1480436788999387E-4	0.0	0.0	52.94500731092698	0.0
33	4.1480436788999387E-4	0.0	0.0	53.36935217927845	0.0
34	4.1480436788999387E-4	0.0	0.0	53.758853480727154	0.0
35	4.1480436788999387E-4	0.0	0.0	54.153539836774485	0.0
36	4.1480436788999387E-4	0.0	0.0	54.52043430017318	0.0
37	4.1480436788999387E-4	0.0	0.0	54.898943285872804	0.0
38	4.1480436788999387E-4	0.0	0.0	55.24240130248572	0.0
39	4.1480436788999387E-4	0.0	0.0	55.58378529725918	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGTC	35	1.2099736E-7	45.000004	32
CTAACGG	35	1.2099736E-7	45.000004	2
ACGTCTC	35	1.2099736E-7	45.000004	34
CGCACGG	35	1.2099736E-7	45.000004	2
ATACGAG	35	1.2099736E-7	45.000004	1
TCACGAC	25	3.8874252E-5	45.0	25
TTGATCG	20	7.0292095E-4	45.0	1
AATCCGG	20	7.0292095E-4	45.0	2
CTCACGA	25	3.8874252E-5	45.0	24
GTTTCAT	20	7.0292095E-4	45.0	10
CGACGGT	25	3.8874252E-5	45.0	28
GCGCGAG	20	7.0292095E-4	45.0	1
CGTTGTT	25	3.8874252E-5	45.0	1
GTGGGCG	20	7.0292095E-4	45.0	5
ATTACGG	25	3.8874252E-5	45.0	2
GGCAACG	20	7.0292095E-4	45.0	1
ATAGCGG	25	3.8874252E-5	45.0	2
CGTCTGG	25	3.8874252E-5	45.0	2
TTACTGA	20	7.0292095E-4	45.0	7
GTTAGCG	25	3.8874252E-5	45.0	1
>>END_MODULE