SRR2935170.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935170.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	289009
Sequences flagged as poor quality	0
Sequence length	51
%GC	37

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8324	2.8801871222003466	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5459	1.888868512745278	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	2740	0.948067361224045	RNA PCR Primer, Index 38 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	2201	0.7615679788518697	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2130	0.737001269856648	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1869	0.64669266355027	RNA PCR Primer, Index 27 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1672	0.5785286963381763	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	1508	0.5217830586590729	RNA PCR Primer, Index 38 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1210	0.41867208287631175	RNA PCR Primer, Index 27 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	814	0.2816521284804279	RNA PCR Primer, Index 27 (95% over 23bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	769	0.26608167911725933	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG	728	0.2518952696974835	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	691	0.23909290022110039	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	561	0.19411160206083547	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	551	0.19065150220235358	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	550	0.19030549221650536	No Hit
GATTTGGGGTTTATGTTTTTATTTTATTATTATTTTTTTTAATGAAGTAAG	524	0.1813092325844524	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	465	0.1608946434194091	No Hit
GCCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	434	0.15016833385811515	RNA PCR Primer, Index 27 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	430	0.1487842939147224	RNA PCR Primer, Index 27 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	429	0.14843828392887418	RNA PCR Primer, Index 38 (95% over 24bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	424	0.14670823399963323	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	414	0.1432481341411513	No Hit
AAGAAAGAAAGAAAAAGAAAGGAAGGAAGGAAGGAAGGAAGAAAGAAAGAA	369	0.1276776847779827	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	351	0.12144950503271525	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGT	333	0.11522132528744779	No Hit
AGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	328	0.11349127535820683	RNA PCR Primer, Index 38 (95% over 24bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTC	318	0.11003117549972492	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAATGG	45	3.8198777E-10	45.000004	2
CATGGGT	20	7.0245145E-4	45.0	4
ATGGGCG	20	7.0245145E-4	45.0	5
CGGGTCA	20	7.0245145E-4	45.0	6
TCCATTG	20	7.0245145E-4	45.0	1
TGCCCTC	20	7.0245145E-4	45.0	42
CATATCC	20	7.0245145E-4	45.0	3
TTGCTAG	35	1.207718E-7	45.0	1
TTGTCGG	20	7.0245145E-4	45.0	2
TCGGGAA	20	7.0245145E-4	45.0	5
TAAGTTA	20	7.0245145E-4	45.0	24
AATGGGT	20	7.0245145E-4	45.0	4
ACTTAGG	20	7.0245145E-4	45.0	30
TCTAGCG	20	7.0245145E-4	45.0	1
AGAACCC	20	7.0245145E-4	45.0	25
CGTTATT	170	0.0	45.0	1
TAGTTCG	20	7.0245145E-4	45.0	1
TGCATAG	20	7.0245145E-4	45.0	1
GTCACTA	20	7.0245145E-4	45.0	24
CGAATTG	20	7.0245145E-4	45.0	41

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content