##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935170.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	289009
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63664453356124	31.0	31.0	34.0	30.0	34.0
2	31.737862834721412	31.0	31.0	34.0	30.0	34.0
3	31.734139767273682	33.0	31.0	34.0	30.0	34.0
4	35.564805940299436	37.0	35.0	37.0	33.0	37.0
5	33.05097419111515	37.0	35.0	37.0	28.0	37.0
6	34.06670380507182	37.0	35.0	37.0	28.0	37.0
7	35.31804199869208	37.0	35.0	37.0	32.0	37.0
8	35.22603102325533	37.0	35.0	37.0	32.0	37.0
9	37.33843236715811	39.0	37.0	39.0	34.0	39.0
10	36.8765574774488	39.0	37.0	39.0	32.0	39.0
11	36.78906885252709	39.0	37.0	39.0	32.0	39.0
12	37.11243248480151	39.0	37.0	39.0	33.0	39.0
13	37.03882924061188	39.0	37.0	39.0	33.0	39.0
14	38.00186499382372	40.0	37.0	41.0	33.0	41.0
15	38.15139320920802	40.0	37.0	41.0	33.0	41.0
16	38.12271590158092	40.0	37.0	41.0	33.0	41.0
17	38.045756360528564	40.0	37.0	41.0	33.0	41.0
18	37.872391517219185	39.0	37.0	40.0	33.0	41.0
19	37.59107847852489	39.0	36.0	40.0	33.0	41.0
20	37.384863447159084	39.0	35.0	40.0	33.0	41.0
21	37.29108782079451	39.0	35.0	40.0	33.0	41.0
22	37.23684383531309	39.0	35.0	40.0	33.0	41.0
23	36.97388316626818	39.0	35.0	40.0	32.0	41.0
24	36.66433225262881	38.0	35.0	40.0	32.0	41.0
25	36.47733461587701	38.0	35.0	40.0	31.0	41.0
26	36.76363019836752	38.0	35.0	40.0	32.0	41.0
27	36.59488804846908	38.0	35.0	40.0	32.0	41.0
28	36.695822621440854	38.0	35.0	40.0	32.0	41.0
29	36.79100996854769	38.0	35.0	40.0	33.0	41.0
30	36.38125802310655	38.0	35.0	40.0	31.0	41.0
31	36.25132781332069	38.0	35.0	40.0	31.0	41.0
32	36.057036286067216	38.0	35.0	40.0	30.0	41.0
33	35.827327176662315	38.0	35.0	40.0	30.0	41.0
34	35.651695967945635	38.0	35.0	40.0	27.0	41.0
35	35.441577943939464	38.0	35.0	40.0	25.0	41.0
36	35.15629963080735	38.0	35.0	40.0	24.0	41.0
37	35.071246916186	38.0	35.0	40.0	24.0	41.0
38	35.06958606825393	38.0	35.0	40.0	23.0	41.0
39	35.03880501991287	38.0	35.0	40.0	23.0	41.0
40	34.89279918618451	38.0	35.0	40.0	23.0	41.0
41	34.76333954997941	38.0	35.0	40.0	22.0	41.0
42	34.801345286824976	38.0	35.0	40.0	22.0	41.0
43	34.628385275199044	38.0	35.0	40.0	21.0	41.0
44	34.509240196672074	38.0	34.0	40.0	20.0	41.0
45	34.49730631226017	38.0	34.0	40.0	20.0	41.0
46	34.36288835295787	38.0	34.0	40.0	20.0	41.0
47	34.27286347483988	38.0	34.0	40.0	20.0	41.0
48	34.25605776982724	38.0	34.0	40.0	20.0	41.0
49	34.247417900480606	38.0	34.0	40.0	20.0	41.0
50	34.126380147331055	38.0	34.0	40.0	20.0	41.0
51	33.235442494870405	36.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	10.0
11	13.0
12	11.0
13	8.0
14	19.0
15	33.0
16	45.0
17	99.0
18	153.0
19	329.0
20	519.0
21	841.0
22	1288.0
23	1940.0
24	2745.0
25	3789.0
26	5059.0
27	5773.0
28	5723.0
29	5560.0
30	5829.0
31	6872.0
32	8463.0
33	11302.0
34	16982.0
35	27529.0
36	32665.0
37	39359.0
38	58761.0
39	47275.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.738395690099615	24.726219598697618	21.773716389455	19.761668321747766
2	36.6590659806442	25.789508285209113	23.062257576753666	14.489168157393022
3	24.737983938216455	25.50474206685605	36.813040424346646	12.944233570580845
4	22.819358566688237	28.016774564113923	34.76293125819611	14.400935611001733
5	17.526789823154296	33.59410952600092	33.77576476857122	15.103335882273564
6	19.70699874398375	33.581653166510385	34.10862637495718	12.602721714548682
7	60.392928939929206	11.119030895231637	23.08232615593286	5.405714008906297
8	56.75670999865056	11.019380019307356	23.86084862409129	8.36306135795079
9	52.366189288222856	13.371209893117516	26.422014539339607	7.8405862793200205
10	21.2948385690411	35.37398489320402	31.30629149957268	12.024885038182202
11	17.53786214270144	26.834804452456495	37.359736201986784	18.267597202855274
12	17.597721870253174	24.099249504340694	41.522582341726384	16.780446283679748
13	18.153067897539525	26.741035746291637	42.3038728897716	12.802023466397241
14	13.002363248203345	30.364106308108052	38.956226276690344	17.67730416699826
15	11.281655588580286	30.344037728928857	42.04090530052697	16.33340138196388
16	11.371618184900816	31.4837946223128	38.78806542356813	18.356521769218258
17	11.93907456169185	31.139860696379696	36.088149503994686	20.832915237933765
18	10.935645602732095	30.767207941621194	41.35753557847679	16.939610877169915
19	12.188893771474245	31.164427405374916	38.874567920030174	17.772110903120662
20	12.211384420554378	33.89859831354733	39.05380109269954	14.836216173198757
21	13.876384472455875	31.844683037552464	39.54548128258982	14.733451207401846
22	11.250514689853949	31.655069565307652	35.524153227062136	21.57026251777626
23	11.373002224844209	30.380714787428765	37.218910137746576	21.02737284998045
24	14.871163181769425	29.38420602818597	35.82518191475006	19.919448875294542
25	12.375739163832268	31.8664816666609	33.409686203543835	22.348092965962998
26	13.591964264088663	32.63842994508821	35.002370168403054	18.767235622420063
27	15.974589026639308	32.65676847433817	34.636291603375675	16.73235089564685
28	13.325536574985556	30.617385617748926	34.77261953779986	21.284458269465656
29	16.930268607552012	27.912279548387765	33.814863896972064	21.342587947088155
30	17.266936323782303	33.48096426062856	32.76368555996526	16.488413855623875
31	17.088741181070485	35.54249175631209	29.119508389012104	18.249258673605322
32	15.747952485908742	33.094125096450284	30.611157438003662	20.546764979637313
33	18.464822894788743	30.446110674754074	31.83983889775059	19.249227532706595
34	17.756886463743342	30.839870038649313	30.987270292620643	20.415973204986695
35	17.8894082883232	29.727447934147378	31.171001595106034	21.212142182423385
36	21.472341691781224	33.03149728901176	30.07207388005218	15.424087139154835
37	19.25891581231034	32.87302471549329	31.470992252836417	16.39706721935995
38	16.911238058330362	31.084845108629832	30.811497219809763	21.192419613230037
39	19.80907168980897	28.02646284371767	33.47681214079839	18.68765332567498
40	18.09078610008685	30.72015058354584	35.09510084461038	16.093962471756935
41	18.69630357532118	31.522547740727795	30.64748848651772	19.133660197433297
42	22.78164347823078	28.444788916608132	31.291759080167054	17.48180852499403
43	23.523142877903457	26.76387240535762	31.240203592275673	18.472781124463253
44	19.476210083423005	27.337902971879767	29.58419980000623	23.601687144691
45	20.257500631468226	25.468757028327836	30.02363248203343	24.250109858170507
46	20.654028075250253	28.304308862353768	33.29204280835545	17.749620254040533
47	16.748959374967562	28.972108135040774	36.38571809182413	17.89321439816753
48	16.823351521924923	26.594327512292004	34.75428100854991	21.828039957233166
49	21.016300530433305	23.229034389932494	36.58501984367269	19.16964523596151
50	18.202547325515813	23.70998827026148	34.47989508977229	23.607569314450416
51	17.073170731707318	22.935963931919076	31.096263438162826	28.89460189821078
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6553.0
1	6480.0
2	6407.0
3	10163.0
4	13919.0
5	9163.0
6	4407.0
7	4199.0
8	3991.0
9	4255.0
10	4519.0
11	4863.5
12	5208.0
13	5068.0
14	4928.0
15	4813.0
16	4698.0
17	4660.0
18	4622.0
19	4043.5
20	3465.0
21	3107.0
22	2749.0
23	2750.0
24	2751.0
25	3848.0
26	4326.5
27	3708.0
28	4222.5
29	4737.0
30	4723.5
31	4710.0
32	4830.0
33	4950.0
34	4999.0
35	5048.0
36	5306.5
37	5565.0
38	5927.5
39	6290.0
40	7381.0
41	8472.0
42	10834.5
43	13197.0
44	15191.5
45	17186.0
46	21997.5
47	26809.0
48	32637.0
49	38465.0
50	36440.0
51	34415.0
52	26273.5
53	18132.0
54	13420.0
55	8708.0
56	6682.0
57	4656.0
58	3955.0
59	3254.0
60	2982.5
61	2711.0
62	2461.5
63	2212.0
64	1635.5
65	1059.0
66	850.0
67	641.0
68	491.5
69	342.0
70	310.0
71	278.0
72	219.5
73	161.0
74	117.0
75	57.0
76	41.0
77	28.5
78	16.0
79	11.0
80	6.0
81	4.0
82	2.0
83	2.0
84	2.0
85	1.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	289009.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.13618229040011
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.41969608491037	25.44698974558896
2	11.892922080557906	8.5952960053713
3	5.081914257339217	5.5092293996217965
4	2.790409581077537	4.033389971467876
5	1.8946411948888255	3.4232549796702036
6	1.298959085949359	2.8163653390582524
7	1.0226004239536943	2.5867012731161787
8	0.7871523234635812	2.2755743880793573
9	0.7422576287667759	2.414012128160071
>10	3.845971927560274	22.563946937186408
>50	0.12370960281262144	3.125309259821643
>100	0.08280562123748049	5.564917953005754
>500	0.007981264697588482	1.870354647589651
>1k	0.006983606610389921	4.805672215183124
>5k	0.0019953161743971205	4.968985757079445
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8324	2.8801871222003466	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5459	1.888868512745278	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	2740	0.948067361224045	RNA PCR Primer, Index 38 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	2201	0.7615679788518697	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2130	0.737001269856648	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1869	0.64669266355027	RNA PCR Primer, Index 27 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1672	0.5785286963381763	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	1508	0.5217830586590729	RNA PCR Primer, Index 38 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1210	0.41867208287631175	RNA PCR Primer, Index 27 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	814	0.2816521284804279	RNA PCR Primer, Index 27 (95% over 23bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	769	0.26608167911725933	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG	728	0.2518952696974835	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	691	0.23909290022110039	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	561	0.19411160206083547	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	551	0.19065150220235358	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	550	0.19030549221650536	No Hit
GATTTGGGGTTTATGTTTTTATTTTATTATTATTTTTTTTAATGAAGTAAG	524	0.1813092325844524	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	465	0.1608946434194091	No Hit
GCCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	434	0.15016833385811515	RNA PCR Primer, Index 27 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	430	0.1487842939147224	RNA PCR Primer, Index 27 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	429	0.14843828392887418	RNA PCR Primer, Index 38 (95% over 24bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	424	0.14670823399963323	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	414	0.1432481341411513	No Hit
AAGAAAGAAAGAAAAAGAAAGGAAGGAAGGAAGGAAGGAAGAAAGAAAGAA	369	0.1276776847779827	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	351	0.12144950503271525	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGT	333	0.11522132528744779	No Hit
AGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	328	0.11349127535820683	RNA PCR Primer, Index 38 (95% over 24bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTC	318	0.11003117549972492	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.460099858481916E-4	0.0	0.0	0.5657263268617932	0.0
2	3.460099858481916E-4	0.0	0.0	3.0005985972755176	0.0
3	3.460099858481916E-4	0.0	0.0	4.056621074084198	0.0
4	3.460099858481916E-4	0.0	0.0	5.32474767221782	0.0
5	3.460099858481916E-4	0.0	0.0	8.694884934379207	0.0
6	3.460099858481916E-4	0.0	0.0	10.340854437059054	0.0
7	3.460099858481916E-4	0.0	0.0	12.039071447601978	0.0
8	3.460099858481916E-4	0.0	0.0	14.662519160302967	0.0
9	3.460099858481916E-4	0.0	0.0	15.583597742630852	0.0
10	3.460099858481916E-4	0.0	0.0	18.20877550526108	0.0
11	3.460099858481916E-4	0.0	0.0	22.752578639419532	0.0
12	3.460099858481916E-4	0.0	0.0	25.79366040503929	0.0
13	3.460099858481916E-4	0.0	0.0	26.974938496725017	0.0
14	3.460099858481916E-4	0.0	0.0	27.411603098865434	0.0
15	3.460099858481916E-4	0.0	0.0	28.30188679245283	0.0
16	3.460099858481916E-4	0.0	0.0	30.00287188288254	0.0
17	3.460099858481916E-4	0.0	0.0	32.0474448892595	0.0
18	3.460099858481916E-4	0.0	0.0	34.13215505399486	0.0
19	3.460099858481916E-4	0.0	0.0	35.48920621849147	0.0
20	3.460099858481916E-4	0.0	0.0	36.83899117328526	0.0
21	3.460099858481916E-4	0.0	0.0	38.37458349047954	0.0
22	3.460099858481916E-4	0.0	0.0	39.95377306589068	0.0
23	3.460099858481916E-4	0.0	0.0	41.35096138874568	0.0
24	3.460099858481916E-4	0.0	0.0	42.21218024352183	0.0
25	3.460099858481916E-4	0.0	0.0	42.931881014086066	0.0
26	3.460099858481916E-4	0.0	0.0	43.52494212982987	0.0
27	3.460099858481916E-4	0.0	0.0	43.99932182042774	0.0
28	3.460099858481916E-4	0.0	0.0	44.511070589497216	0.0
29	3.460099858481916E-4	0.0	0.0	45.050154147448694	0.0
30	3.460099858481916E-4	0.0	0.0	45.586469625513395	0.0
31	3.460099858481916E-4	0.0	0.0	46.049084976592425	0.0
32	3.460099858481916E-4	0.0	0.0	46.479175389001725	0.0
33	3.460099858481916E-4	0.0	0.0	46.86082440339228	0.0
34	3.460099858481916E-4	0.0	0.0	47.23036306827815	0.0
35	3.460099858481916E-4	0.0	0.0	47.62792854201772	0.0
36	3.460099858481916E-4	0.0	0.0	47.98224276752627	0.0
37	3.460099858481916E-4	0.0	0.0	48.30472407433678	0.0
38	3.460099858481916E-4	0.0	0.0	48.623053261317125	0.0
39	3.460099858481916E-4	0.0	0.0	48.9804815766983	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAATGG	45	3.8198777E-10	45.000004	2
CATGGGT	20	7.0245145E-4	45.0	4
ATGGGCG	20	7.0245145E-4	45.0	5
CGGGTCA	20	7.0245145E-4	45.0	6
TCCATTG	20	7.0245145E-4	45.0	1
TGCCCTC	20	7.0245145E-4	45.0	42
CATATCC	20	7.0245145E-4	45.0	3
TTGCTAG	35	1.207718E-7	45.0	1
TTGTCGG	20	7.0245145E-4	45.0	2
TCGGGAA	20	7.0245145E-4	45.0	5
TAAGTTA	20	7.0245145E-4	45.0	24
AATGGGT	20	7.0245145E-4	45.0	4
ACTTAGG	20	7.0245145E-4	45.0	30
TCTAGCG	20	7.0245145E-4	45.0	1
AGAACCC	20	7.0245145E-4	45.0	25
CGTTATT	170	0.0	45.0	1
TAGTTCG	20	7.0245145E-4	45.0	1
TGCATAG	20	7.0245145E-4	45.0	1
GTCACTA	20	7.0245145E-4	45.0	24
CGAATTG	20	7.0245145E-4	45.0	41
>>END_MODULE