SRR2935168.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935168.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	978277
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37321	3.8149726508954007	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	5290	0.540746639244304	No Hit
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	3764	0.38475810021088097	Illumina PCR Primer Index 5 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3476	0.35531858563576574	No Hit
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	3235	0.3306834362864506	RNA PCR Primer, Index 33 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	2827	0.2889774573050373	Illumina PCR Primer Index 5 (96% over 26bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCG	1906	0.19483234298670007	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1683	0.1720371632983296	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1508	0.15414856937247837	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1477	0.1509797327341847	No Hit
TCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1343	0.1372821808138186	RNA PCR Primer, Index 33 (96% over 27bp)
CGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	1337	0.1366688575935037	Illumina PCR Primer Index 5 (95% over 24bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGC	1169	0.11949580742468646	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGT	1126	0.11510032434576302	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1016	0.1038560653066565	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCG	20	7.0327707E-4	45.000004	1
CGACGGT	80	0.0	45.000004	28
CGGGTAT	25	3.8903785E-5	45.0	6
AATTGCG	25	3.8903785E-5	45.0	1
CGGTCTA	85	0.0	45.0	31
TTGCACG	25	3.8903785E-5	45.0	1
CTTACGG	30	2.1651686E-6	44.999996	2
CGTTATT	745	0.0	44.09396	1
CGTTTTT	11835	0.0	43.326996	1
GTATCGG	75	0.0	42.000004	2
TACGGGT	65	0.0	41.53846	4
GGCGTAG	55	6.002665E-11	40.909092	1
TAGCGAG	55	6.002665E-11	40.909092	1
GTTCACG	55	6.002665E-11	40.909092	1
CGCACGG	55	6.002665E-11	40.909092	2
TCGTTAG	50	1.0804797E-9	40.5	1
TTGTGCG	50	1.0804797E-9	40.5	1
TCACGAC	95	0.0	40.263157	25
TATGCGG	90	0.0	40.0	2
TACGCGG	45	1.927765E-8	40.0	2