##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935168.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	978277
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83538506987285	33.0	31.0	34.0	30.0	34.0
2	31.947123360765918	33.0	31.0	34.0	30.0	34.0
3	31.90965544523688	33.0	31.0	34.0	30.0	34.0
4	35.684299027780476	37.0	35.0	37.0	33.0	37.0
5	33.324468427653926	37.0	35.0	37.0	30.0	37.0
6	34.270666692562536	37.0	35.0	37.0	29.0	37.0
7	35.53040089872296	37.0	35.0	37.0	32.0	37.0
8	35.509273958193845	37.0	35.0	37.0	33.0	37.0
9	37.56502912774194	39.0	37.0	39.0	35.0	39.0
10	37.050910938312974	39.0	37.0	39.0	33.0	39.0
11	36.97390105256487	39.0	37.0	39.0	33.0	39.0
12	37.211445224614295	39.0	37.0	39.0	34.0	39.0
13	37.173911887941756	39.0	37.0	39.0	34.0	39.0
14	38.115447874170606	40.0	38.0	41.0	33.0	41.0
15	38.23869926411436	40.0	38.0	41.0	33.0	41.0
16	38.247361432395934	40.0	38.0	41.0	33.0	41.0
17	38.2207360491967	40.0	38.0	41.0	33.0	41.0
18	38.047204421651536	39.0	37.0	41.0	34.0	41.0
19	37.792830660436664	39.0	37.0	41.0	34.0	41.0
20	37.54427426996648	39.0	35.0	41.0	33.0	41.0
21	37.44458675814723	39.0	35.0	40.0	33.0	41.0
22	37.44038753849881	39.0	35.0	40.0	33.0	41.0
23	37.230223137209606	39.0	35.0	40.0	33.0	41.0
24	36.97787640923787	39.0	35.0	40.0	32.0	41.0
25	36.76173415096133	38.0	35.0	40.0	32.0	41.0
26	37.01242592844358	39.0	35.0	40.0	33.0	41.0
27	36.95936427003804	39.0	35.0	40.0	33.0	41.0
28	36.973360305925624	39.0	35.0	40.0	33.0	41.0
29	36.90938660522531	39.0	35.0	40.0	33.0	41.0
30	36.6311136825255	39.0	35.0	40.0	31.0	41.0
31	36.39751522319343	39.0	35.0	40.0	31.0	41.0
32	35.981038090438595	38.0	35.0	40.0	30.0	41.0
33	35.76318466037738	39.0	35.0	40.0	28.0	41.0
34	35.47649694309485	39.0	35.0	40.0	25.0	41.0
35	35.18841493769147	38.0	35.0	40.0	23.0	41.0
36	35.080466984299946	38.0	35.0	40.0	23.0	41.0
37	34.99690578435351	38.0	35.0	40.0	22.0	41.0
38	34.86054052175406	38.0	35.0	40.0	22.0	41.0
39	34.7333638632003	38.0	34.0	40.0	21.0	41.0
40	34.670991958310374	38.0	34.0	40.0	21.0	41.0
41	34.62465130019412	38.0	34.0	40.0	20.0	41.0
42	34.74669444339384	38.0	35.0	40.0	21.0	41.0
43	34.67851845642901	38.0	35.0	40.0	20.0	41.0
44	34.56434220573519	38.0	35.0	40.0	18.0	41.0
45	34.490801685003326	38.0	34.0	40.0	20.0	41.0
46	34.398208278432385	38.0	34.0	40.0	20.0	41.0
47	34.33720510652913	38.0	34.0	40.0	20.0	41.0
48	34.321470299311954	38.0	34.0	40.0	20.0	41.0
49	34.327408290289966	38.0	34.0	40.0	19.0	41.0
50	34.25831231849466	38.0	34.0	40.0	18.0	41.0
51	33.3064357027713	37.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	27.0
10	30.0
11	27.0
12	24.0
13	35.0
14	59.0
15	98.0
16	154.0
17	263.0
18	432.0
19	821.0
20	1436.0
21	2405.0
22	3730.0
23	5782.0
24	9226.0
25	14639.0
26	19182.0
27	20807.0
28	19947.0
29	18897.0
30	19513.0
31	22268.0
32	27926.0
33	37331.0
34	55766.0
35	76028.0
36	100253.0
37	132746.0
38	205708.0
39	182656.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.21188477292219	20.57065636828833	21.577221993361796	23.640236865427685
2	41.026314632767615	21.372065376166464	24.3481140822078	13.253505908858127
3	27.07126918040596	22.01390812622601	38.2537870153341	12.66103567803393
4	24.163708233966453	23.33388191688039	37.460146768246624	15.042263080906531
5	18.702984941892737	30.90576595381472	35.275080575338066	15.11616852895448
6	21.42164233647525	29.722256579680394	36.4484701163372	12.407630967507158
7	66.20875273567711	6.119739092302078	23.007900625283025	4.663607546737785
8	65.41961019220528	5.372404748348371	23.628072621558108	5.579912437888247
9	59.077132550392164	8.935812658377944	25.47274442719189	6.514310364037998
10	23.61825945003307	33.81434910562142	30.429418252703478	12.13797319164204
11	20.71826282331078	21.98988630009701	38.95583766152122	18.336013215070988
12	20.838167512882343	19.61417880620724	41.50041348207103	18.047240198839386
13	21.10302092352166	19.51686485525061	43.93224005061961	15.44787417060812
14	16.05751745160113	22.989909810820453	43.0407747498919	17.911797987686516
15	13.761746417425739	23.98226678128996	44.78588375276123	17.470103048523068
16	16.148187067671017	24.672562065754384	40.743879289812604	18.435371576761998
17	16.04944202920032	23.654036637884772	40.32630839731487	19.97021293560004
18	15.537317140237377	23.313233368463123	43.66135562831386	17.488093862985636
19	15.545085901028033	24.814341950183845	42.062319772416195	17.57825237637193
20	15.22912222202914	27.22398666226437	41.63984229415595	15.907048821550543
21	15.424159006089278	25.336177790135107	43.545846421821224	15.693816781954395
22	13.875108992647277	23.407480703318182	41.761280291778306	20.956130012256242
23	13.418183193512675	23.912654595784222	42.2273037186809	20.441858492022195
24	15.238526511407303	24.045643514055833	40.350534664517305	20.365295310019555
25	14.128718144247488	26.776260711434492	38.19204581115574	20.902975333162285
26	12.982825927625816	27.44151196440272	39.43218536263247	20.143476745338997
27	15.627066771476791	25.157700733023468	40.58124641589243	18.633986079607308
28	13.520812612378702	24.85083468179258	40.068303762635736	21.56004894319298
29	15.727856220681872	25.032787237152665	39.37974622729554	19.85961031486992
30	20.077442278618427	25.210957632654146	37.00086989676748	17.710730191959946
31	18.23491710425575	28.47956151478569	34.79730178671276	18.488219594245802
32	18.141487533694445	27.420352313301855	35.522658715271845	18.915501437731848
33	20.798505944635313	24.769467134564135	33.853192909574695	20.57883401122586
34	18.042129172003428	26.608210149068206	34.865482884704434	20.484177794223925
35	17.840448053056548	27.91438416726551	34.49074239709203	19.754425382585914
36	22.384355351296207	27.48761342646306	31.288172981681058	18.83985824055968
37	21.154028971344516	27.139143616787475	34.12847281495936	17.578354596908646
38	20.976369678526634	24.84122595134098	35.093843563735014	19.08856080639737
39	21.60584374364316	24.461681098502776	33.480394612159955	20.452080545694113
40	21.06008829809962	25.992433635872047	34.82163027445192	18.125847791576412
41	18.429749447242447	27.16152991432897	32.988816051077556	21.419904587351027
42	19.858996991649605	24.589252328328275	34.6117715125675	20.939979167454617
43	21.146260210553862	22.57172559510241	34.899317882358474	21.382696311985256
44	21.6637005674262	22.88901814107865	32.82311656105581	22.624164730439333
45	22.058987382919153	22.585627588096212	32.97246076520249	22.38292426378214
46	22.316992017598288	24.247937956223033	34.29304787907719	19.142022147101486
47	17.93479760844832	25.220055260422154	37.68012536326623	19.165021767863294
48	18.73252667700457	23.43068476515343	36.72671441728672	21.110074140555284
49	21.260338329532434	20.95061010327341	36.99678107529871	20.792270491895444
50	19.692684178407546	20.82436774042526	35.93062087731798	23.552327203849217
51	18.673954309464495	20.92638383607097	33.35098341267351	27.048678441791026
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5182.0
1	7390.0
2	9598.0
3	33542.0
4	57486.0
5	35398.0
6	13310.0
7	12805.0
8	12300.0
9	12414.0
10	12528.0
11	12318.0
12	12108.0
13	11609.0
14	11110.0
15	10608.5
16	10107.0
17	9768.5
18	9430.0
19	9241.0
20	9052.0
21	8997.0
22	8942.0
23	9848.5
24	10755.0
25	10874.0
26	11706.0
27	12419.0
28	13664.0
29	14909.0
30	16147.0
31	17385.0
32	18931.0
33	20477.0
34	21968.0
35	23459.0
36	24762.0
37	26065.0
38	27252.0
39	28439.0
40	31219.0
41	33999.0
42	40288.5
43	46578.0
44	53017.5
45	59457.0
46	72334.5
47	85212.0
48	99553.0
49	113894.0
50	111904.0
51	109914.0
52	87501.5
53	65089.0
54	51391.0
55	37693.0
56	31303.0
57	24913.0
58	21893.5
59	18874.0
60	16655.0
61	14436.0
62	12842.5
63	11249.0
64	9566.0
65	7883.0
66	6485.0
67	5087.0
68	4013.5
69	2940.0
70	2493.0
71	2046.0
72	1704.5
73	1363.0
74	1029.0
75	526.5
76	358.0
77	315.0
78	272.0
79	191.5
80	111.0
81	87.5
82	64.0
83	62.5
84	61.0
85	42.0
86	23.0
87	14.0
88	5.0
89	4.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	978277.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.92936103884888
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.49047916680304	27.8307867432478
2	11.126912849727688	8.663272151497122
3	4.72180645617602	5.514507248641316
4	2.673098319736531	4.162480383254548
5	1.7928000839943468	3.4896280870147267
6	1.345701057779507	3.1432369397195554
7	1.062402157977283	2.895104602344487
8	0.8890168411342825	2.768708606250675
9	0.7650451494540976	2.6804446950706806
>10	3.9689875130890138	24.43737203223938
>50	0.10655984816890218	2.778830874705444
>100	0.04990685308116509	3.606859735408109
>500	0.003237201279160571	0.8373123500220181
>1k	0.003506968052423951	2.7165130513005824
>5k	2.697667732633809E-4	0.5555477651594728
>10k+	2.697667732633809E-4	3.919394734124136
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37321	3.8149726508954007	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	5290	0.540746639244304	No Hit
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	3764	0.38475810021088097	Illumina PCR Primer Index 5 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3476	0.35531858563576574	No Hit
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	3235	0.3306834362864506	RNA PCR Primer, Index 33 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	2827	0.2889774573050373	Illumina PCR Primer Index 5 (96% over 26bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCG	1906	0.19483234298670007	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1683	0.1720371632983296	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1508	0.15414856937247837	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1477	0.1509797327341847	No Hit
TCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1343	0.1372821808138186	RNA PCR Primer, Index 33 (96% over 27bp)
CGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	1337	0.1366688575935037	Illumina PCR Primer Index 5 (95% over 24bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGC	1169	0.11949580742468646	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGT	1126	0.11510032434576302	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1016	0.1038560653066565	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.31514591470513975	0.0
2	0.0	0.0	0.0	1.3389868104841471	0.0
3	0.0	0.0	0.0	1.9080485383996557	0.0
4	0.0	0.0	0.0	2.598957146084391	0.0
5	0.0	0.0	0.0	4.6594165047323	0.0
6	0.0	0.0	0.0	5.980412500753877	0.0
7	0.0	0.0	0.0	7.2109433217790055	0.0
8	0.0	0.0	0.0	9.064610534644073	0.0
9	0.0	0.0	0.0	9.86233960320032	0.0
10	0.0	0.0	0.0	12.177225877742194	0.0
11	0.0	0.0	0.0	15.822307996610366	0.0
12	0.0	0.0	0.0	18.58952014613448	0.0
13	0.0	0.0	0.0	19.72488364747408	0.0
14	0.0	0.0	0.0	20.231284186380748	0.0
15	0.0	0.0	0.0	21.084927888522373	0.0
16	0.0	0.0	0.0	22.674661675578594	0.0
17	0.0	0.0	0.0	24.59231894442985	0.0
18	0.0	0.0	0.0	26.394467006788467	0.0
19	0.0	0.0	0.0	27.629393310892517	0.0
20	0.0	0.0	0.0	28.921563115559294	0.0
21	0.0	0.0	0.0	30.363690447593065	0.0
22	0.0	0.0	0.0	31.900167335018608	0.0
23	0.0	0.0	0.0	33.08623222257091	0.0
24	0.0	0.0	0.0	33.91237860033508	0.0
25	0.0	0.0	0.0	34.56771446124155	0.0
26	0.0	0.0	0.0	35.1578336197212	0.0
27	0.0	0.0	0.0	35.71759327879527	0.0
28	0.0	0.0	0.0	36.21080736846517	0.0
29	0.0	0.0	0.0	36.67723967751465	0.0
30	0.0	0.0	0.0	37.21430637743707	0.0
31	0.0	0.0	0.0	37.68605415439594	0.0
32	0.0	0.0	0.0	38.071732239437296	0.0
33	0.0	0.0	0.0	38.43185519029886	0.0
34	1.022205367191501E-4	0.0	0.0	38.79453365457841	0.0
35	1.022205367191501E-4	0.0	0.0	39.17111411185176	0.0
36	2.044410734383002E-4	0.0	0.0	39.50731745712104	0.0
37	2.044410734383002E-4	0.0	0.0	39.8492451524466	0.0
38	2.044410734383002E-4	0.0	0.0	40.21570577658475	0.0
39	2.044410734383002E-4	0.0	0.0	40.688169097300666	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCG	20	7.0327707E-4	45.000004	1
CGACGGT	80	0.0	45.000004	28
CGGGTAT	25	3.8903785E-5	45.0	6
AATTGCG	25	3.8903785E-5	45.0	1
CGGTCTA	85	0.0	45.0	31
TTGCACG	25	3.8903785E-5	45.0	1
CTTACGG	30	2.1651686E-6	44.999996	2
CGTTATT	745	0.0	44.09396	1
CGTTTTT	11835	0.0	43.326996	1
GTATCGG	75	0.0	42.000004	2
TACGGGT	65	0.0	41.53846	4
GGCGTAG	55	6.002665E-11	40.909092	1
TAGCGAG	55	6.002665E-11	40.909092	1
GTTCACG	55	6.002665E-11	40.909092	1
CGCACGG	55	6.002665E-11	40.909092	2
TCGTTAG	50	1.0804797E-9	40.5	1
TTGTGCG	50	1.0804797E-9	40.5	1
TCACGAC	95	0.0	40.263157	25
TATGCGG	90	0.0	40.0	2
TACGCGG	45	1.927765E-8	40.0	2
>>END_MODULE