FastQCFastQC Report
Sat 14 Jan 2017
SRR2935167.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935167.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4337286
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3644868.40355005411218No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTT142440.32840813356555226No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCT118560.2733506621421783No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA109280.2519547938503479No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGT98790.22776916255925939No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCC97690.2252330143781157No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTC89490.20632718248231727No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC89490.20632718248231727No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCG77820.1794209558696383No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA56880.13114191685768475No Hit
CGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG56450.13015051347778311TruSeq Adapter, Index 20 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT55120.12708407976785482No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA43750.1008695299318514No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1443350.044.572871
CGTTATT109250.044.5057221
CGGTCTA5800.042.67241731
TAGTACG1450.041.8965531
CGACGGT6150.040.60975628
GTTATTT122650.040.0101932
GTTTTTT1619200.039.6904372
TCACGAC6350.038.9763825
CGCATCG1450.038.79310621
CGTAAGG4100.038.4146352
CGTTCTG12200.038.176231
CTCACGA6600.037.84090824
CGTTTCT18950.037.519791
AATCGGC301.1404443E-437.49999628
CGTTTTC16500.037.363641
ACGGTCT6650.037.2180530
TACGGGA17800.036.9101144
GCCGCAT1650.036.8181819
CGCCGCA1600.036.562518
ATGGGCG5750.035.2173925
Produced by FastQC (version 0.11.5)