##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935167.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4337286
Sequences flagged as poor quality	0
Sequence length	51
%GC	31
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22240820642217	33.0	31.0	34.0	30.0	34.0
2	32.38528494547051	34.0	31.0	34.0	31.0	34.0
3	32.253439593330945	34.0	31.0	34.0	30.0	34.0
4	35.94933467610851	37.0	35.0	37.0	35.0	37.0
5	33.37574925886833	37.0	35.0	37.0	30.0	37.0
6	34.475385298548446	37.0	35.0	37.0	29.0	37.0
7	35.764319669028055	37.0	35.0	37.0	32.0	37.0
8	35.78928712563571	37.0	35.0	37.0	35.0	37.0
9	37.93470917066571	39.0	38.0	39.0	35.0	39.0
10	37.553788705656025	39.0	37.0	39.0	35.0	39.0
11	37.418766712640114	39.0	37.0	39.0	35.0	39.0
12	37.63908859134491	39.0	37.0	39.0	35.0	39.0
13	37.63014359671001	39.0	37.0	39.0	35.0	39.0
14	38.858471403545906	40.0	38.0	41.0	35.0	41.0
15	38.98266312159263	40.0	38.0	41.0	35.0	41.0
16	38.93810207581423	40.0	38.0	41.0	35.0	41.0
17	38.8464159384463	40.0	38.0	41.0	35.0	41.0
18	38.34037068341816	39.0	38.0	40.0	35.0	41.0
19	37.711181600659955	38.0	37.0	40.0	34.0	41.0
20	37.07694811916945	38.0	35.0	40.0	34.0	41.0
21	36.931553971769446	37.0	35.0	40.0	33.0	41.0
22	36.89336326910423	37.0	35.0	40.0	33.0	41.0
23	36.72119915541654	37.0	35.0	40.0	33.0	41.0
24	36.43155304953374	37.0	35.0	40.0	33.0	41.0
25	36.23732606058258	36.0	35.0	40.0	33.0	41.0
26	36.46305177938462	37.0	35.0	40.0	33.0	41.0
27	36.379058010009025	37.0	35.0	40.0	33.0	41.0
28	36.338473183460806	37.0	35.0	40.0	33.0	41.0
29	36.2756610931352	37.0	35.0	40.0	33.0	41.0
30	35.87188416904027	36.0	35.0	40.0	31.0	41.0
31	35.42072577183059	36.0	35.0	40.0	30.0	41.0
32	34.5892998524884	36.0	35.0	40.0	21.0	41.0
33	33.58481824809339	36.0	33.0	40.0	15.0	41.0
34	32.68461775405173	36.0	33.0	40.0	10.0	41.0
35	32.04558357461325	36.0	31.0	40.0	8.0	41.0
36	31.668430903565042	36.0	30.0	40.0	7.0	41.0
37	31.520394781437055	36.0	29.0	40.0	7.0	41.0
38	31.374742177481494	36.0	27.0	40.0	7.0	41.0
39	31.18515910640894	36.0	25.0	40.0	7.0	41.0
40	31.034524354630985	36.0	24.0	40.0	7.0	41.0
41	30.846826794451644	35.0	23.0	40.0	7.0	41.0
42	30.764874393802945	35.0	23.0	40.0	7.0	41.0
43	30.63912594189085	35.0	23.0	40.0	7.0	41.0
44	30.50156180616173	35.0	22.0	40.0	7.0	41.0
45	30.47361760326619	35.0	23.0	40.0	7.0	41.0
46	30.409494554889854	35.0	23.0	40.0	7.0	41.0
47	30.372549792658358	35.0	23.0	40.0	7.0	41.0
48	30.298392358723866	35.0	23.0	40.0	7.0	41.0
49	30.254462122165798	35.0	22.0	40.0	7.0	41.0
50	30.183705893501145	35.0	22.0	40.0	7.0	41.0
51	29.551058657418487	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	16.0
9	61.0
10	80.0
11	78.0
12	111.0
13	161.0
14	269.0
15	459.0
16	863.0
17	1565.0
18	3189.0
19	5842.0
20	9970.0
21	15589.0
22	24057.0
23	39479.0
24	71738.0
25	134262.0
26	205755.0
27	226669.0
28	198203.0
29	156537.0
30	127561.0
31	116018.0
32	117191.0
33	134811.0
34	191729.0
35	284892.0
36	364379.0
37	380161.0
38	642011.0
39	883267.0
40	308.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.6384029091003	14.141723649305119	16.739269672325044	44.480603769269536
2	56.53943502918646	15.395249471674221	17.656963363725612	10.408352135413711
3	19.736674039941107	15.908473639967482	54.458041272814384	9.89681104727703
4	17.471432596328672	17.513601823813325	53.387786740371745	11.627178839486259
5	14.91755443380953	25.61023183622201	48.92137617855959	10.550837551408875
6	16.999340140355052	22.290160252286796	51.153509360461825	9.556990246896332
7	52.517680411206456	4.149507318631974	40.43111291254485	2.901699357616722
8	54.135696838991024	3.600684852232479	39.84832911641058	2.4152891923659174
9	49.24000861368146	6.589719930850767	40.93495333256787	3.235318122899897
10	18.88293739449047	29.235471214026465	44.50534274198197	7.376248649501093
11	16.38118860504011	18.33282379810785	53.7717595749969	11.514228021855141
12	16.291109232824397	16.592680307454938	54.33019634859219	12.78601411112848
13	15.377288931373215	16.44961849414588	55.62086521386876	12.552227360612141
14	11.97965732488012	19.18273316539421	55.60269717053475	13.234912339190913
15	11.418223285252575	20.475684564033823	55.83862811905879	12.267464031654818
16	14.304590474319657	20.94023774314168	53.7400577227326	11.015114059806063
17	14.1688373789508	20.57397183399942	53.15268580397973	12.104504983070058
18	13.584854676403632	20.661907008207436	53.762260547263885	11.990977768125044
19	13.191728652433804	21.492656928779887	52.92650749800681	12.389106920779493
20	13.624072749641137	22.37830292952782	52.65218848837729	11.34543583245375
21	13.860672319049286	21.039308913454175	53.99344198192141	11.106576785575127
22	13.795401087223668	18.807452402262616	54.39685093397115	13.000295576542568
23	12.059107930627585	20.483200785007032	54.43443203883719	13.023259245528193
24	12.123825821031863	20.949529267841687	53.76472752776736	13.161917383359087
25	12.995891901064399	21.70811424471432	52.48980583710643	12.80618801711485
26	12.759038716838134	22.23189801179816	52.0612198503857	12.947843420978003
27	12.84842641227717	21.13270833419793	53.101847560894065	12.91701769263083
28	12.132079830566857	21.204366048261516	53.06560369779627	13.597950423375355
29	12.787743303070167	20.94634755466898	52.822940428645936	13.442968713614919
30	13.691442067689334	20.62310394103594	52.50071127428535	13.184742716989382
31	13.566871080210067	21.3345165617393	51.69456199106999	13.404050366980641
32	13.921124869330729	22.308143848480363	50.50453209679971	13.266199185389205
33	14.481959455751822	22.993641645950945	48.078983032246434	14.445415866050798
34	13.865075994527453	24.151439402428153	46.915905476373936	15.067579126670458
35	14.416134882504867	25.19490298772089	44.53679097942815	15.852171150346091
36	15.290483495900432	25.3643637980064	43.02347135973971	16.321681346353458
37	15.167918371073524	26.826914342286855	41.92979204046032	16.075375246179295
38	15.288708192173631	26.775361366531975	41.29238883486125	16.64354160643315
39	15.792848338799887	25.584178677633894	40.879895861144504	17.743077122421717
40	16.702518579591015	25.871224539954245	40.97039023942622	16.455866641028514
41	16.005262276916948	26.511486676230252	40.176114740877125	17.307136305975675
42	16.28018073975292	26.708038160268888	40.25941568068142	16.752365419296765
43	16.59440949939663	26.371744911449234	40.504246203732016	16.529599385422127
44	16.855471370806537	26.24383081954937	39.75377690103904	17.14692090860506
45	16.21550896113376	25.678546445864995	40.23689007365435	17.869054519346893
46	16.2489630612323	25.632895778604407	40.556121961982676	17.562019198180614
47	15.60807841585729	26.015254700750656	41.0944309413767	17.282235942015355
48	15.522010768946295	25.7682108120147	41.13662322475392	17.57315519428509
49	15.886270815436196	25.375799520714104	41.02016791145431	17.717761752395393
50	15.01515002699845	25.07913935119796	41.56751480073023	18.338195821073363
51	14.917485266131864	24.94619907472092	41.090326992501765	19.045988666645457
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13234.0
1	13204.0
2	13174.0
3	280939.0
4	548704.0
5	347406.0
6	146108.0
7	139580.5
8	133053.0
9	132803.5
10	132554.0
11	130397.0
12	128240.0
13	124350.5
14	120461.0
15	113314.5
16	106168.0
17	98551.5
18	90935.0
19	84780.0
20	78625.0
21	74261.5
22	69898.0
23	66527.5
24	63157.0
25	63588.0
26	64848.5
27	65678.0
28	71496.0
29	77314.0
30	84212.0
31	91110.0
32	98254.5
33	105399.0
34	111880.5
35	118362.0
36	123543.5
37	128725.0
38	138016.5
39	147308.0
40	151836.5
41	156365.0
42	167214.0
43	178063.0
44	190462.5
45	202862.0
46	224912.0
47	246962.0
48	248123.5
49	249285.0
50	235241.0
51	221197.0
52	191136.0
53	161075.0
54	140553.5
55	120032.0
56	109309.0
57	98586.0
58	87859.5
59	77133.0
60	70557.5
61	63982.0
62	54834.0
63	45686.0
64	37264.0
65	28842.0
66	23646.5
67	18451.0
68	14609.5
69	10768.0
70	8675.0
71	6582.0
72	5470.5
73	4359.0
74	3363.0
75	1780.0
76	1193.0
77	924.5
78	656.0
79	520.0
80	384.0
81	258.0
82	132.0
83	84.0
84	36.0
85	33.0
86	30.0
87	19.0
88	8.0
89	8.0
90	8.0
91	7.5
92	7.0
93	4.0
94	1.0
95	3.0
96	5.0
97	3.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4337286.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.089259679108466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.39882217047678	23.096529563323532
2	7.6237754248270715	4.435399661360003
3	2.8610772219776	2.4967985481626602
4	1.6519378348997507	1.922145946125722
5	1.07958151102374	1.5702113459466933
6	0.8171142187311454	1.4261548617697273
7	0.6340497256428508	1.2910825983084655
8	0.5370047789746679	1.249685717161309
9	0.45020663954088724	1.1786560062177285
>10	4.075440822527347	25.62425757488199
>50	0.6840031076124602	13.682005448337687
>100	0.1757630098925981	7.794662221416942
>500	0.00750986457722317	1.4496142598326272
>1k	0.0027233574837032897	1.6514343653632912
>5k	6.602078748371612E-4	1.492537341518373
>10k+	3.301039374185806E-4	9.638824540273237
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	364486	8.40355005411218	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTT	14244	0.32840813356555226	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCT	11856	0.2733506621421783	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10928	0.2519547938503479	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGT	9879	0.22776916255925939	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCC	9769	0.2252330143781157	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTC	8949	0.20632718248231727	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	8949	0.20632718248231727	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCG	7782	0.1794209558696383	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	5688	0.13114191685768475	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG	5645	0.13015051347778311	TruSeq Adapter, Index 20 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT	5512	0.12708407976785482	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	4375	0.1008695299318514	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0750303300266573E-4	0.0	0.0	0.0765455632854278	0.0
2	2.0750303300266573E-4	0.0	0.0	0.2700075577215798	0.0
3	2.0750303300266573E-4	0.0	0.0	0.49067550537363686	0.0
4	2.0750303300266573E-4	0.0	0.0	0.7598299950706502	0.0
5	2.0750303300266573E-4	0.0	0.0	1.3742695316840992	0.0
6	2.0750303300266573E-4	0.0	0.0	2.229089804084859	0.0
7	2.0750303300266573E-4	0.0	0.0	2.9335626011289087	0.0
8	2.0750303300266573E-4	0.0	0.0	3.7248638895382964	0.0
9	2.0750303300266573E-4	0.0	0.0	4.288165456462867	0.0
10	2.0750303300266573E-4	0.0	0.0	4.991139620490786	0.0
11	2.0750303300266573E-4	0.0	0.0	5.78774376418802	0.0
12	2.0750303300266573E-4	0.0	0.0	6.320496273476087	0.0
13	2.0750303300266573E-4	0.0	0.0	6.5982967228815435	0.0
14	2.0750303300266573E-4	0.0	0.0	6.773567618091129	0.0
15	2.0750303300266573E-4	0.0	0.0	6.944227334789543	0.0
16	2.3055892555851748E-4	0.0	0.0	7.190625658533931	0.0
17	2.3055892555851748E-4	0.0	0.0	7.506030268697983	0.0
18	2.3055892555851748E-4	0.0	0.0	7.838542351138477	0.0
19	2.536148181143692E-4	0.0	0.0	8.06734902886275	0.0
20	2.536148181143692E-4	0.0	0.0	8.324745013356278	0.0
21	2.536148181143692E-4	0.0	0.0	8.592216422896715	0.0
22	2.76670710670221E-4	0.0	0.0	8.877118087209375	0.0
23	2.76670710670221E-4	0.0	0.0	9.137326890594718	0.0
24	2.76670710670221E-4	0.0	0.0	9.332771691790672	0.0
25	2.997266032260727E-4	0.0	0.0	9.50486087382755	0.0
26	2.997266032260727E-4	0.0	0.0	9.66549127726417	0.0
27	2.997266032260727E-4	0.0	0.0	9.841084954969537	0.0
28	2.997266032260727E-4	0.0	0.0	10.017900594980363	0.0
29	2.997266032260727E-4	0.0	0.0	10.19928130171725	0.0
30	2.997266032260727E-4	0.0	0.0	10.397792536623133	0.0
31	3.2278249578192444E-4	0.0	0.0	10.568175582610877	0.0
32	3.2278249578192444E-4	0.0	0.0	10.74985601595099	0.0
33	3.2278249578192444E-4	0.0	0.0	10.911385599197287	0.0
34	3.2278249578192444E-4	0.0	0.0	11.070886263898668	0.0
35	3.2278249578192444E-4	0.0	0.0	11.235320889607003	0.0
36	3.6889428089362795E-4	0.0	0.0	11.38617559459994	0.0
37	3.6889428089362795E-4	0.0	0.0	11.544661800028866	2.3055892555851747E-5
38	3.6889428089362795E-4	0.0	0.0	11.733697063094294	2.3055892555851747E-5
39	3.6889428089362795E-4	0.0	0.0	12.034553405055604	2.3055892555851747E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	144335	0.0	44.57287	1
CGTTATT	10925	0.0	44.505722	1
CGGTCTA	580	0.0	42.672417	31
TAGTACG	145	0.0	41.896553	1
CGACGGT	615	0.0	40.609756	28
GTTATTT	12265	0.0	40.010193	2
GTTTTTT	161920	0.0	39.690437	2
TCACGAC	635	0.0	38.97638	25
CGCATCG	145	0.0	38.793106	21
CGTAAGG	410	0.0	38.414635	2
CGTTCTG	1220	0.0	38.17623	1
CTCACGA	660	0.0	37.840908	24
CGTTTCT	1895	0.0	37.51979	1
AATCGGC	30	1.1404443E-4	37.499996	28
CGTTTTC	1650	0.0	37.36364	1
ACGGTCT	665	0.0	37.21805	30
TACGGGA	1780	0.0	36.910114	4
GCCGCAT	165	0.0	36.81818	19
CGCCGCA	160	0.0	36.5625	18
ATGGGCG	575	0.0	35.217392	5
>>END_MODULE