FastQCFastQC Report
Sat 14 Jan 2017
SRR2935166.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935166.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5212043
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4694529.007063065289369No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130110.2496333971151044No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTT123280.23652913070747883No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGT109510.21010954821362757No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCT100250.19234300254238118No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCC94220.18077364288821102No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTC85470.16398560027229248No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA81440.15625350750176084No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCG76480.14673708563033727No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTC73180.1404055952723337No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA65280.12524839108196154No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA59000.11319937306733656No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1875700.044.4711
CGTTATT139100.043.8838961
GTTATTT151350.040.64422
GTTTTTT2103750.039.735832
CGGTCTA4350.038.2758631
TACGCGG3500.036.6428572
CGACGGT4750.036.47368228
CGTTTCT17600.036.1789781
CCCGTAG250.00210779135.99999632
CGTAAGG6050.035.7024772
TAGTGCG2200.034.7727281
CGTTAGG4150.034.6987952
CGTTTTC15300.034.5588231
GCGTAAG3000.034.51
TGCGACG1700.034.4117621
TCACGAC4850.034.329925
TACGGGA27750.034.2162174
ACGGGAT32000.034.171875
TAAGGGA57800.034.061424
GACCGAT11450.033.7991269
Produced by FastQC (version 0.11.5)