FastQCFastQC Report
Sat 14 Jan 2017
SRR2935165.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935165.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448036
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240401.660179719288747No Hit
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC112390.7761547364844521TruSeq Adapter, Index 16 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC78100.5393512315992144TruSeq Adapter, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT67480.4660105135507681TruSeq Adapter, Index 27 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCC50360.34778140874950625No Hit
TCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC44560.3077271559546862TruSeq Adapter, Index 16 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38880.26850161183837973No Hit
CGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG36080.24916507600639765TruSeq Adapter, Index 27 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC35750.24688612714048544TruSeq Adapter, Index 27 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG22520.15552099533437014TruSeq Adapter, Index 27 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG19430.13418174686264706TruSeq Adapter, Index 16 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG18550.12810454988688125TruSeq Adapter, Index 27 (95% over 22bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16550.11429273857832263No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCG15730.10862989594181359No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC15350.10600565179318747No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG302.1658943E-645.0000041
TCGATAG302.1658943E-645.0000041
GCTAGCG351.2122291E-745.0000041
GGCGCGA600.045.0000048
CTCCGCG207.0338947E-445.040
CGATTAT207.0338947E-445.010
AGCGCTA253.8913106E-545.045
GGACGAT253.8913106E-545.08
ATGCGTG207.0338947E-445.010
TACTACG253.8913106E-545.01
CCGAACC207.0338947E-445.018
CGGGTAT650.044.9999966
CGTTAGG2050.043.902442
CGTTTTT80250.043.5140231
ACGGGTA1200.043.1250045
GCGTAAG1100.042.9545441
TACGTAG900.042.51
CGTTATT6300.042.4999961
TATAGCG750.042.01
CGGTCTA1500.042.031
Produced by FastQC (version 0.11.5)