##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935165.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1448036
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.564800875116365	31.0	31.0	34.0	30.0	34.0
2	31.663397180733075	31.0	31.0	34.0	30.0	34.0
3	31.690961412561567	31.0	31.0	34.0	30.0	34.0
4	35.517441555320445	37.0	35.0	37.0	33.0	37.0
5	33.070631531260275	37.0	35.0	37.0	28.0	37.0
6	34.04767422909375	36.0	35.0	37.0	28.0	37.0
7	35.37644160780533	37.0	35.0	37.0	32.0	37.0
8	35.37398863011693	37.0	35.0	37.0	33.0	37.0
9	37.35760782190498	39.0	37.0	39.0	34.0	39.0
10	36.72371819485151	39.0	37.0	39.0	32.0	39.0
11	36.70190658243303	39.0	37.0	39.0	32.0	39.0
12	37.04583035228406	39.0	37.0	39.0	33.0	39.0
13	37.00086116643509	39.0	37.0	39.0	33.0	39.0
14	37.804945457157146	39.0	37.0	41.0	33.0	41.0
15	37.94591087514399	39.0	37.0	41.0	33.0	41.0
16	37.92774834327324	39.0	37.0	41.0	33.0	41.0
17	37.950340323030645	39.0	37.0	41.0	33.0	41.0
18	37.909535398291204	39.0	37.0	40.0	33.0	41.0
19	37.79211083149866	39.0	37.0	40.0	33.0	41.0
20	37.66848407083802	39.0	36.0	40.0	33.0	41.0
21	37.58383286050899	39.0	36.0	40.0	33.0	41.0
22	37.55075633478726	39.0	36.0	40.0	33.0	41.0
23	37.13052576040927	39.0	35.0	40.0	32.0	41.0
24	36.81963639025549	39.0	35.0	40.0	31.0	41.0
25	36.42664754191194	38.0	35.0	40.0	31.0	41.0
26	36.61934993328895	38.0	35.0	40.0	31.0	41.0
27	36.521404163984876	38.0	35.0	40.0	31.0	41.0
28	36.430460983014235	38.0	35.0	40.0	31.0	41.0
29	36.55838666994467	38.0	35.0	40.0	31.0	41.0
30	36.273723857694144	38.0	35.0	40.0	30.0	41.0
31	35.89196539312559	38.0	35.0	40.0	30.0	41.0
32	35.755344480385844	38.0	35.0	40.0	29.0	41.0
33	35.59104884132715	38.0	35.0	40.0	27.0	41.0
34	35.64916963390413	38.0	35.0	40.0	27.0	41.0
35	35.62059921162181	38.0	35.0	40.0	27.0	41.0
36	35.558905303459305	38.0	35.0	40.0	27.0	41.0
37	35.539420290655755	38.0	35.0	40.0	27.0	41.0
38	35.4923075116917	38.0	35.0	40.0	26.0	41.0
39	35.48068141952272	38.0	35.0	40.0	26.0	41.0
40	35.367318215845465	38.0	35.0	40.0	25.0	41.0
41	35.172750539351235	38.0	34.0	40.0	25.0	41.0
42	35.299759812601344	38.0	35.0	40.0	25.0	41.0
43	35.159222560765066	38.0	34.0	40.0	25.0	41.0
44	35.16307191257676	38.0	35.0	40.0	25.0	41.0
45	35.210597664698945	38.0	35.0	40.0	24.0	41.0
46	35.165198240927715	38.0	35.0	40.0	24.0	41.0
47	35.188194906756465	38.0	35.0	40.0	25.0	41.0
48	35.277646412105774	38.0	35.0	40.0	25.0	41.0
49	35.422585488206096	39.0	35.0	40.0	26.0	41.0
50	35.41828725252687	39.0	35.0	40.0	26.0	41.0
51	34.34790364327959	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	12.0
10	21.0
11	29.0
12	23.0
13	29.0
14	50.0
15	83.0
16	137.0
17	265.0
18	495.0
19	896.0
20	1612.0
21	2676.0
22	4398.0
23	6484.0
24	9640.0
25	13661.0
26	18097.0
27	20803.0
28	22810.0
29	25329.0
30	29946.0
31	37626.0
32	49201.0
33	66644.0
34	89882.0
35	125497.0
36	178014.0
37	258325.0
38	316957.0
39	168329.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.37363711951913	23.70017043775155	23.831866058578655	17.09432638415067
2	33.76697816905105	24.298705280807937	26.30528522771533	15.629031322425686
3	27.65649472803162	24.194149869202146	33.72112295550663	14.428232447259598
4	25.484034927308436	25.123201356872343	32.804640216127225	16.588123499692
5	18.846699943924047	33.29903400191708	30.390749953730435	17.463516100428443
6	22.92332511070167	31.195702316793227	31.966125151584627	13.914847420920474
7	71.04236358764561	5.707731023261853	16.92223121524603	6.327674173846506
8	67.7671687720471	4.946838338273358	18.580891635290836	8.705101254388703
9	61.23701344441713	8.656967091978377	21.066741434605216	9.039278028999279
10	23.318135736956815	36.490322063816095	26.5987862180222	13.592755981204888
11	18.0963732945866	22.905231637887454	37.406942921308584	21.59145214621736
12	19.784521931775174	19.42244529832131	40.44858000767937	20.344452762224144
13	20.524420663574663	20.76813007411418	43.02365410804704	15.683795154264121
14	15.512045280642194	25.696598703347156	38.725625605993216	20.06573041001743
15	12.053291492752942	25.991066520445628	42.96039601225384	18.995245974547593
16	13.917264487899473	27.56664889546945	36.61007046786129	21.906016148769783
17	13.39945968194161	26.6797924913469	35.840407282691864	24.080340544019624
18	13.585228544041723	25.527058719534594	40.809551696228546	20.078161040195134
19	13.332955810490898	27.246145814054344	37.76674060589654	21.654157769558218
20	13.30574654221304	29.48289959641887	38.07792071467836	19.133433146689725
21	13.631429052868851	28.65453621318807	39.570701280907386	18.143333453035698
22	12.319859451008124	24.956838089660753	35.91775342601979	26.805549033311326
23	11.52236546605195	26.431939537414816	37.81881113452981	24.226883862003433
24	15.639183003737475	23.94519197036538	35.375294536876154	25.040330489020988
25	12.511981746310175	28.3432870453497	33.720225187771575	25.42450602056855
26	11.970904038297391	26.652099809673242	37.085542072158425	24.29145407987094
27	15.32600018231591	25.055454422403862	36.22285633782585	23.395689057454373
28	10.50105107884058	23.80507114464005	38.41313337513708	27.280744401382286
29	13.257474261689625	21.457546635580886	38.1029891522034	27.18198995052609
30	15.86500611863241	23.445204400995557	35.23807419152563	25.45171528884641
31	12.473723028985468	26.75064708335981	34.171180826996014	26.604449060658713
32	12.489744730103396	24.745033963244005	33.96655884245972	28.79866246419288
33	12.83331353640379	22.061053730708352	33.74812504661486	31.357507686272996
34	11.918281037211782	19.362225801016	37.35577016041038	31.363723001361844
35	10.973069730310572	19.819327696272744	36.747497990381454	32.460104583035225
36	12.598858039441009	20.069114303788027	37.41177705526658	29.920250601504385
37	12.137612600791693	20.80873679936134	37.07228273330221	29.981367866544755
38	12.463502288617134	21.067846379509902	36.36808753373535	30.100563798137614
39	16.395448731937602	17.841407257830607	34.77054437873092	30.992599631500873
40	16.38619481836087	17.647972840454244	38.87327386888171	27.092558472303175
41	14.063945923996366	20.070288307749255	33.960274468314324	31.90549129994006
42	15.691598827653456	18.05963387650583	37.44603034731181	28.802736948528906
43	17.57200787825717	17.09432638415067	35.43751674682121	29.89614899077095
44	16.75780160161764	17.461029974392904	33.96262247623678	31.81854594775268
45	17.561718078832296	14.762616399039802	34.41544270998788	33.26022281214003
46	20.06724970926137	16.603454610244498	36.60081655428456	26.72847912620957
47	12.613774795654251	17.8927181368419	40.92833327348215	28.565173794021696
48	13.789021819899505	17.01442505573066	38.36838310649735	30.828170017872488
49	17.70404879436699	13.714368979776745	39.95321939509791	28.628362830758352
50	16.589021267427054	13.089867931460267	38.28613377015488	32.0349770309578
51	15.933719879892488	13.161827468377856	33.08398409984282	37.82046855188683
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5667.0
1	7553.0
2	9439.0
3	23700.5
4	37962.0
5	23998.0
6	10034.0
7	9896.0
8	9758.0
9	10105.5
10	10453.0
11	10702.0
12	10951.0
13	10662.0
14	10373.0
15	9982.5
16	9592.0
17	9390.5
18	9189.0
19	9233.5
20	9278.0
21	10331.0
22	11384.0
23	11575.0
24	11766.0
25	12219.0
26	13637.5
27	14603.0
28	15315.0
29	16027.0
30	17957.0
31	19887.0
32	22000.5
33	24114.0
34	26447.5
35	28781.0
36	30370.5
37	31960.0
38	35321.5
39	38683.0
40	45436.5
41	52190.0
42	63376.5
43	74563.0
44	92312.0
45	110061.0
46	144409.5
47	178758.0
48	210994.5
49	243231.0
50	227808.0
51	212385.0
52	161048.5
53	109712.0
54	79709.0
55	49706.0
56	37206.5
57	24707.0
58	20396.5
59	16086.0
60	13608.5
61	11131.0
62	9752.0
63	8373.0
64	6958.5
65	5544.0
66	4529.5
67	3515.0
68	2925.5
69	2336.0
70	1823.0
71	1310.0
72	1103.0
73	896.0
74	655.5
75	339.5
76	264.0
77	207.0
78	150.0
79	109.0
80	68.0
81	54.5
82	41.0
83	25.5
84	10.0
85	7.0
86	4.0
87	2.5
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1448036.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.209434919231214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.67916545925296	21.476982403284577
2	12.23123100194341	7.879220778783588
3	5.724472078441135	5.531460325725178
4	3.410064339533615	4.393449816583965
5	2.231329502371025	3.593493119499004
6	1.6016917504295791	3.095375171767863
7	1.2354669635358704	2.785558492980817
8	1.0153388531937193	2.6162792570327964
9	0.7877432257600696	2.28354877468658
>10	4.755553844851603	26.216159660045374
>50	0.2135809251585158	4.720196827331879
>100	0.09669996713831609	5.744539706850061
>500	0.01059725303412659	2.4383871311915466
>1k	0.005960954831696207	3.323289127707954
>5k	6.623283146329119E-4	1.3933437576135073
>10k+	4.415522097552746E-4	2.5087156489153273
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24040	1.660179719288747	No Hit
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	11239	0.7761547364844521	TruSeq Adapter, Index 16 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	7810	0.5393512315992144	TruSeq Adapter, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT	6748	0.4660105135507681	TruSeq Adapter, Index 27 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCC	5036	0.34778140874950625	No Hit
TCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	4456	0.3077271559546862	TruSeq Adapter, Index 16 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3888	0.26850161183837973	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	3608	0.24916507600639765	TruSeq Adapter, Index 27 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	3575	0.24688612714048544	TruSeq Adapter, Index 27 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	2252	0.15552099533437014	TruSeq Adapter, Index 27 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	1943	0.13418174686264706	TruSeq Adapter, Index 16 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	1855	0.12810454988688125	TruSeq Adapter, Index 27 (95% over 22bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1655	0.11429273857832263	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCG	1573	0.10862989594181359	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC	1535	0.10600565179318747	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5058575891759597	0.0
2	0.0	0.0	0.0	2.5402683358701026	0.0
3	0.0	0.0	0.0	3.7347137778342527	0.0
4	0.0	0.0	0.0	5.267410478745004	0.0
5	0.0	0.0	0.0	9.397487355286748	0.0
6	0.0	0.0	0.0	11.698880414575328	0.0
7	0.0	0.0	0.0	14.090395542652255	0.0
8	0.0	0.0	0.0	17.63878798593405	0.0
9	0.0	0.0	0.0	18.918797598954722	0.0
10	0.0	0.0	0.0	22.51587667709919	0.0
11	0.0	0.0	0.0	27.55304426133052	0.0
12	0.0	0.0	0.0	31.7335342491485	0.0
13	6.905905654279313E-5	0.0	0.0	33.30953097851158	0.0
14	6.905905654279313E-5	0.0	0.0	33.990798571306236	0.0
15	6.905905654279313E-5	0.0	0.0	35.206168907402855	0.0
16	6.905905654279313E-5	0.0	0.0	37.50189912405493	0.0
17	6.905905654279313E-5	0.0	0.0	40.38884392376985	0.0
18	6.905905654279313E-5	0.0	0.0	43.28587134574002	0.0
19	1.3811811308558626E-4	0.0	0.0	45.10350571394634	0.0
20	1.3811811308558626E-4	0.0	0.0	47.15476687043692	0.0
21	2.762362261711725E-4	0.0	0.0	49.375775187909696	0.0
22	2.762362261711725E-4	0.0	0.0	51.74505329977984	0.0
23	2.762362261711725E-4	0.0	0.0	53.592383062299554	0.0
24	2.762362261711725E-4	0.0	0.0	54.86679889174026	0.0
25	2.762362261711725E-4	0.0	0.0	55.843293951255355	0.0
26	3.4529528271396565E-4	0.0	0.0	56.67953006693204	0.0
27	3.4529528271396565E-4	0.0	0.0	57.4752285164181	0.0
28	3.4529528271396565E-4	0.0	0.0	58.165680963732946	0.0
29	3.4529528271396565E-4	0.0	0.0	58.81849622523197	0.0
30	3.4529528271396565E-4	0.0	0.0	59.523382015364255	0.0
31	3.4529528271396565E-4	0.0	0.0	60.06515031394247	0.0
32	3.4529528271396565E-4	0.0	0.0	60.5306083550409	0.0
33	3.4529528271396565E-4	0.0	0.0	60.9435815131668	0.0
34	4.1435433925675883E-4	0.0	0.0	61.34944158846879	0.0
35	4.1435433925675883E-4	0.0	0.0	61.76262192376433	0.0
36	4.1435433925675883E-4	0.0	0.0	62.12739186042336	0.0
37	5.52472452342345E-4	0.0	0.0	62.49644345858805	0.0
38	5.52472452342345E-4	0.0	0.0	62.84629663903384	0.0
39	5.52472452342345E-4	0.0	0.0	63.283785762232434	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	30	2.1658943E-6	45.000004	1
TCGATAG	30	2.1658943E-6	45.000004	1
GCTAGCG	35	1.2122291E-7	45.000004	1
GGCGCGA	60	0.0	45.000004	8
CTCCGCG	20	7.0338947E-4	45.0	40
CGATTAT	20	7.0338947E-4	45.0	10
AGCGCTA	25	3.8913106E-5	45.0	45
GGACGAT	25	3.8913106E-5	45.0	8
ATGCGTG	20	7.0338947E-4	45.0	10
TACTACG	25	3.8913106E-5	45.0	1
CCGAACC	20	7.0338947E-4	45.0	18
CGGGTAT	65	0.0	44.999996	6
CGTTAGG	205	0.0	43.90244	2
CGTTTTT	8025	0.0	43.514023	1
ACGGGTA	120	0.0	43.125004	5
GCGTAAG	110	0.0	42.954544	1
TACGTAG	90	0.0	42.5	1
CGTTATT	630	0.0	42.499996	1
TATAGCG	75	0.0	42.0	1
CGGTCTA	150	0.0	42.0	31
>>END_MODULE