SRR2935163.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935163.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	329367
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8997	2.7316033482407165	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCC	1931	0.5862760993056377	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1864	0.5659340492520502	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGC	1332	0.40441209957281693	No Hit
CCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGC	1233	0.37435444352348596	No Hit
CTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGCT	874	0.26535748875874027	Illumina Single End Adapter 1 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	682	0.20706385278428016	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	664	0.20159882441167454	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	627	0.19036515497909626	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	546	0.1657725273023709	No Hit
TCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGC	546	0.1657725273023709	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	540	0.16395085117816904	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCG	527	0.1600038862423983	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGC	467	0.14178712500037952	No Hit
ACTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGC	433	0.1314642936299022	No Hit
CCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCTGTCTCTTA	394	0.11962339882259002	No Hit
AAGTTAGGGAAATATATATAAATATATTAAAATACCAATTTTTTTTCTCTG	380	0.11537282119945227	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	378	0.11476559582471832	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCTGTCTCTTA	344	0.10444276445424103	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGGGT	65	0.0	45.000004	4
GCGAAGG	45	3.8380676E-10	45.0	2
CTGACTA	20	7.0259505E-4	45.0	22
ACGGGTT	20	7.0259505E-4	45.0	5
TGTGCGG	25	3.8847247E-5	45.0	2
TCTAGCG	20	7.0259505E-4	45.0	1
AGAACGG	20	7.0259505E-4	45.0	2
CGTTATT	320	0.0	45.0	1
TACGCGG	25	3.8847247E-5	45.0	2
ATGAGCG	20	7.0259505E-4	45.0	1
TACGAGG	20	7.0259505E-4	45.0	2
CTACGAA	25	3.8847247E-5	45.0	11
TACGAAT	25	3.8847247E-5	45.0	12
GAAGTGC	20	7.0259505E-4	45.0	9
CGAATGG	35	1.208391E-7	45.0	2
CGAATAT	25	3.8847247E-5	45.0	14
TCCGTTA	20	7.0259505E-4	45.0	32
CGATGCA	20	7.0259505E-4	45.0	10
GGACCTA	20	7.0259505E-4	45.0	8
GCTACGA	25	3.8847247E-5	45.0	10

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content