##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935162.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1508220
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91210433491135	33.0	31.0	34.0	30.0	34.0
2	32.05530360292265	33.0	31.0	34.0	30.0	34.0
3	31.98694288631632	33.0	31.0	34.0	30.0	34.0
4	35.73496572118126	37.0	35.0	37.0	35.0	37.0
5	33.177226134118364	37.0	35.0	37.0	28.0	37.0
6	34.232655050324226	37.0	35.0	37.0	28.0	37.0
7	35.54210658922438	37.0	35.0	37.0	32.0	37.0
8	35.54367930408031	37.0	35.0	37.0	33.0	37.0
9	37.655369243213855	39.0	37.0	39.0	35.0	39.0
10	37.11668125339804	39.0	37.0	39.0	33.0	39.0
11	36.99268541724682	39.0	37.0	39.0	33.0	39.0
12	37.27328837967936	39.0	37.0	39.0	34.0	39.0
13	37.25471814456777	39.0	37.0	39.0	34.0	39.0
14	38.320955165692006	40.0	38.0	41.0	33.0	41.0
15	38.44435294585671	40.0	38.0	41.0	34.0	41.0
16	38.420973730622855	40.0	38.0	41.0	33.0	41.0
17	38.3512385461007	40.0	38.0	41.0	33.0	41.0
18	38.03684542042938	39.0	37.0	40.0	34.0	41.0
19	37.587419607219104	38.0	37.0	40.0	34.0	41.0
20	37.14888013685006	38.0	35.0	40.0	33.0	41.0
21	37.0428684144223	38.0	35.0	40.0	33.0	41.0
22	37.01973054328944	38.0	35.0	40.0	33.0	41.0
23	36.793810584662715	38.0	35.0	40.0	33.0	41.0
24	36.49055774356526	38.0	35.0	40.0	32.0	41.0
25	36.28566853642042	38.0	35.0	40.0	31.0	41.0
26	36.541965363143305	38.0	35.0	40.0	33.0	41.0
27	36.442965217276	38.0	35.0	40.0	32.0	41.0
28	36.43028271737545	38.0	35.0	40.0	32.0	41.0
29	36.40636777128005	38.0	35.0	40.0	32.0	41.0
30	36.024235191152485	38.0	35.0	40.0	31.0	41.0
31	35.637048308602196	38.0	35.0	40.0	29.0	41.0
32	35.052325257588414	38.0	35.0	40.0	24.0	41.0
33	34.396474652239064	38.0	34.0	40.0	18.0	41.0
34	33.78525148850964	38.0	33.0	40.0	15.0	41.0
35	33.36928365888266	38.0	33.0	40.0	10.0	41.0
36	33.08890546472	38.0	33.0	40.0	10.0	41.0
37	32.98839890732121	38.0	33.0	40.0	10.0	41.0
38	32.90813939610932	38.0	33.0	40.0	10.0	41.0
39	32.77161422073703	38.0	33.0	40.0	10.0	41.0
40	32.633660871756106	38.0	32.0	40.0	9.0	41.0
41	32.47881012053944	38.0	31.0	40.0	8.0	41.0
42	32.47062829030248	38.0	32.0	40.0	8.0	41.0
43	32.36656654864675	38.0	31.0	40.0	8.0	41.0
44	32.25058148015542	37.0	31.0	40.0	8.0	41.0
45	32.21501239872167	37.0	31.0	40.0	8.0	41.0
46	32.09534020235775	37.0	31.0	40.0	8.0	41.0
47	31.9961935261434	37.0	31.0	40.0	8.0	41.0
48	31.914872498707084	37.0	30.0	40.0	8.0	41.0
49	31.86460861147578	37.0	30.0	40.0	8.0	41.0
50	31.758933709936215	37.0	30.0	40.0	7.0	41.0
51	30.906485128164327	35.0	27.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	12.0
9	18.0
10	33.0
11	41.0
12	40.0
13	79.0
14	121.0
15	206.0
16	319.0
17	611.0
18	1193.0
19	2166.0
20	3639.0
21	5831.0
22	9271.0
23	14711.0
24	24486.0
25	40059.0
26	54655.0
27	55937.0
28	48453.0
29	40256.0
30	36278.0
31	37261.0
32	42377.0
33	53705.0
34	77789.0
35	108945.0
36	137583.0
37	168800.0
38	264964.0
39	278276.0
40	102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.202901433477873	18.607563883253107	19.914932834732333	32.27460184853669
2	47.27758549813687	19.889870178090728	20.59527124690032	12.237273076872073
3	22.858203710334035	19.993701184177375	45.073530386813594	12.074564718674994
4	20.623251249817663	21.00907029478458	44.98090464255878	13.386773812838976
5	16.921801859145216	28.871583721207784	41.139157417352905	13.067457002294095
6	18.466603015475197	27.773003938417475	42.794287305565504	10.96610574054183
7	59.575990240150645	5.740674437416292	30.88780151436793	3.7955338080651364
8	60.13718157828434	5.267533914150455	30.468963413825566	4.12632109373964
9	55.19101987773667	7.950696847939956	31.908673800904374	4.949609473418997
10	23.30164034424686	29.202238400233387	37.342827969394385	10.153293286125367
11	18.710135126175224	22.181843497632972	44.457837715983075	14.650183660208723
12	18.06566681253398	19.023153120897483	47.287265783506385	15.62391428306215
13	17.9988330614897	19.560475262229648	48.653114267149355	13.787577409131293
14	14.35413931654533	22.349259391865907	47.493137605919564	15.8034636856692
15	13.264974605826735	23.006723157099096	48.76079086605402	14.967511371020143
16	15.384625585126837	23.269682141862592	45.80253543912692	15.54315683388365
17	15.435016111707842	23.360186179735052	44.72762594316479	16.477171765392317
18	14.999801090026654	22.954277227460185	46.71917889963002	15.326742782883136
19	14.576520666746232	24.409900412406678	45.46273090132739	15.550848019519698
20	14.778082773070242	24.90346235960271	45.80890055827399	14.509554309053055
21	15.01869753749453	23.62407341104083	47.32844014798902	14.02878890347562
22	14.30169338690642	21.823341422338917	45.928246542281634	17.946718648473034
23	13.307342430149447	22.891023855936137	46.51184840407898	17.289785309835434
24	14.291747888239117	22.930408030658658	45.59871901977165	17.179125061330573
25	13.880932489955047	24.66748882788983	43.90274628369866	17.54883239845646
26	13.235005503175929	24.60940711567278	44.4265425468433	17.72904483430799
27	13.895983344604899	23.59887814775033	45.00298364960019	17.502154858044584
28	12.289254883239845	24.1333492461312	44.62883398973624	18.948561880892708
29	13.549018047764916	22.81550436938908	44.48190582275796	19.15357176008805
30	14.498945777141268	23.837636419090053	43.617111561973715	18.046306241794962
31	13.53721605601305	26.74523610613836	41.6601689408707	18.057378896977895
32	14.560740475527442	25.776279322645234	41.167601543541394	18.495378658285926
33	14.976860139767409	24.938072694964923	40.3058572356818	19.77920992958587
34	14.253358263383326	26.66089827743963	39.3885507419342	19.697192717242842
35	15.5129888212595	25.791926907215128	37.21214411690602	21.482940154619353
36	16.05767063160547	27.123363965469228	35.783771598307936	21.035193804617364
37	15.645595470156874	29.044569094694406	36.32401108591585	18.98582434923287
38	16.448528729230482	27.66552624948615	37.081990691013246	18.80395433027012
39	17.16162098367612	25.939186590815666	37.36537109970694	19.533821325801277
40	18.495842781557066	25.78774979777486	36.84681279919375	18.86959462147432
41	17.638010369839943	26.36856691994537	34.39955709379268	21.593865616422008
42	17.539947752980332	25.615095940910475	35.35538581904497	21.48957048706422
43	17.265054169816075	24.97109175054037	36.44276034000345	21.321093739640105
44	16.959992574027662	25.1289599660527	35.83548819137792	22.075559268541724
45	17.360464653697736	23.988012358939677	36.251077429022295	22.400445558340294
46	17.693174735781252	24.791277134635532	36.506080014851946	21.009468114731273
47	15.599448356340586	25.79026932410391	38.02369680815796	20.586585511397544
48	16.145655143148876	25.14851944676506	37.283353887363916	21.42247152272215
49	17.18502605720651	23.578655633793478	38.20629616368964	21.030022145310365
50	16.383882987893013	23.218297065414863	37.84713105515111	22.55068889154102
51	16.145920356446673	23.174006444683137	36.072124756335285	24.60794844253491
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8192.0
1	9534.0
2	10876.0
3	66551.0
4	122226.0
5	78406.5
6	34587.0
7	33143.5
8	31700.0
9	32252.0
10	32804.0
11	32844.5
12	32885.0
13	31972.0
14	31059.0
15	29359.0
16	27659.0
17	25977.0
18	24295.0
19	22796.5
20	21298.0
21	20492.0
22	19686.0
23	20026.0
24	20366.0
25	20121.0
26	20202.0
27	20528.0
28	21481.0
29	22434.0
30	24098.5
31	25763.0
32	28175.5
33	30588.0
34	32913.0
35	35238.0
36	37431.5
37	39625.0
38	41735.0
39	43845.0
40	49103.5
41	54362.0
42	61015.0
43	67668.0
44	75543.5
45	83419.0
46	99051.0
47	114683.0
48	124834.5
49	134986.0
50	127945.5
51	120905.0
52	99490.0
53	78075.0
54	64922.5
55	51770.0
56	44616.5
57	37463.0
58	34188.5
59	30914.0
60	27657.0
61	24400.0
62	21757.0
63	19114.0
64	16243.5
65	13373.0
66	10640.0
67	7907.0
68	6257.0
69	4607.0
70	3982.0
71	3357.0
72	3047.5
73	2738.0
74	2027.5
75	995.0
76	673.0
77	513.5
78	354.0
79	347.5
80	341.0
81	250.0
82	159.0
83	101.0
84	43.0
85	32.5
86	22.0
87	25.0
88	28.0
89	18.0
90	8.0
91	5.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1508220.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.18985138910287
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.06744959958725	30.202618967449997
2	9.482997918637667	7.43274558309164
3	3.8034208231905913	4.471664904931757
4	2.063622362056061	3.2349221476882595
5	1.2772804804639775	2.502821610579259
6	0.8707432156836917	2.047457833242806
7	0.7093215787950573	1.9458745082043483
8	0.5156464062874151	1.6166484825383007
9	0.43984019319695306	1.55135446257086
>10	3.5176737178069026	26.674949804991606
>50	0.18542018773796556	4.8557597639315695
>100	0.05994264780595061	3.991829993371718
>500	0.0022718761515324237	0.6079000939781933
>1k	0.0040194731911727495	2.9236186583755206
>5k	1.747597039640326E-4	0.4131200278139322
>10k+	1.747597039640326E-4	5.5267131572402315
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	80696	5.3504130697113155	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6032	0.39994165307448515	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCC	4818	0.31944941719377806	No Hit
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	3490	0.23139860232592063	Illumina PCR Primer Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	3322	0.22025964381854105	Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	2576	0.1707973637798199	Illumina PCR Primer Index 10 (95% over 24bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCG	2074	0.13751309490657862	No Hit
CGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	1996	0.13234143559958098	Illumina PCR Primer Index 10 (95% over 22bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGT	1883	0.12484915993687924	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCT	1804	0.11961119730543289	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTT	1728	0.11457214464733262	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTC	1725	0.11437323467398655	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1654	0.10966569863812971	No Hit
TCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	1647	0.1092015753669889	Illumina PCR Primer Index 10 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1636	0.10847223879805333	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.326066488973757E-4	0.0	0.0	0.18757210486533796	0.0
2	1.326066488973757E-4	0.0	0.0	0.8816353051942024	0.0
3	1.326066488973757E-4	0.0	0.0	1.3252708490803728	0.0
4	1.326066488973757E-4	0.0	0.0	1.8878545570274894	0.0
5	1.326066488973757E-4	0.0	0.0	3.3084695866650753	0.0
6	1.326066488973757E-4	0.0	0.0	4.373367280635451	0.0
7	1.326066488973757E-4	0.0	0.0	5.349683733142379	0.0
8	1.326066488973757E-4	0.0	0.0	6.676015435413932	0.0
9	1.326066488973757E-4	0.0	0.0	7.2660487196828045	0.0
10	1.326066488973757E-4	0.0	0.0	8.670750951452705	0.0
11	1.326066488973757E-4	0.0	0.0	10.712893344472292	0.0
12	1.326066488973757E-4	0.0	0.0	12.212608240177163	0.0
13	1.326066488973757E-4	0.0	0.0	12.820145602100489	0.0
14	1.326066488973757E-4	0.0	0.0	13.091922929015661	0.0
15	1.326066488973757E-4	0.0	0.0	13.527137950696847	0.0
16	1.326066488973757E-4	0.0	0.0	14.391733301507738	0.0
17	1.326066488973757E-4	0.0	0.0	15.503706355836682	0.0
18	1.326066488973757E-4	0.0	0.0	16.638487753775973	0.0
19	1.9890997334606357E-4	0.0	0.0	17.40734110408296	0.0
20	1.9890997334606357E-4	0.0	0.0	18.182758483510362	0.0
21	1.9890997334606357E-4	0.0	0.0	19.090252085239555	0.0
22	1.9890997334606357E-4	0.0	0.0	20.047804696927503	0.0
23	1.9890997334606357E-4	0.0	0.0	20.945684316611636	0.0
24	1.9890997334606357E-4	0.0	0.0	21.544071819761044	0.0
25	1.9890997334606357E-4	0.0	0.0	22.022052485711633	0.0
26	1.9890997334606357E-4	0.0	0.0	22.435321107000306	0.0
27	1.9890997334606357E-4	0.0	0.0	22.849053851560118	0.0
28	1.9890997334606357E-4	0.0	0.0	23.237790242802774	0.0
29	1.9890997334606357E-4	0.0	0.0	23.644561138295476	0.0
30	1.9890997334606357E-4	0.0	0.0	24.05066900054369	0.0
31	1.9890997334606357E-4	0.0	0.0	24.412154725437933	0.0
32	3.315166222434393E-4	0.0	0.0	24.726896606595854	0.0
33	3.315166222434393E-4	0.0	0.0	25.020023603983503	0.0
34	3.315166222434393E-4	0.0	0.0	25.305857235681795	0.0
35	3.315166222434393E-4	0.0	0.0	25.595204943575872	0.0
36	3.9781994669212714E-4	0.0	0.0	25.859688904801686	0.0
37	3.9781994669212714E-4	0.0	0.0	26.142804100197583	0.0
38	3.9781994669212714E-4	0.0	0.0	26.433411571256183	0.0
39	3.9781994669212714E-4	0.0	0.0	26.843696542944663	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	25	3.8913866E-5	45.000004	1
CGCGTCA	30	2.1659544E-6	45.000004	39
AGTCGAA	25	3.8913866E-5	45.000004	25
CGGCCTA	20	7.033989E-4	45.0	45
CGTTCGC	20	7.033989E-4	45.0	44
TACCGGT	20	7.033989E-4	45.0	40
CGTTTTT	33195	0.0	44.254402	1
CGTTATT	2360	0.0	44.141945	1
GCGTAAG	100	0.0	42.750004	1
CGGTCTA	245	0.0	40.408165	31
TAGCCGT	45	1.9290383E-8	40.0	44
ACGATAG	85	0.0	39.705883	1
GACGGTC	250	0.0	39.6	29
ATCGGTT	40	3.4591721E-7	39.375	10
GTTATTT	2710	0.0	39.271217	2
TCACGAC	260	0.0	38.942307	25
CGACGGT	255	0.0	38.82353	28
TCGTTAG	35	6.2495874E-6	38.571426	1
GCGCGAC	105	0.0	38.571426	9
CTACGGG	280	0.0	38.571426	3
>>END_MODULE