SRR2935161.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935161.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	173624
Sequences flagged as poor quality	0
Sequence length	51
%GC	36

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6985	4.023061327927015	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2549	1.4681150071418698	No Hit
CCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	1116	0.6427682808828272	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCC	1091	0.6283693498594665	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	893	0.5143298161544487	No Hit
GCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	688	0.39625858176288986	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	522	0.300649679767774	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	476	0.2741556466847901	No Hit
CTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGCT	400	0.23038289637377318	Illumina Single End Adapter 1 (95% over 21bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	367	0.2113763074229369	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCG	348	0.2004331198451827	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	299	0.17221121503939546	No Hit
TCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	266	0.15320462608855917	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGC	229	0.13189420817398517	No Hit
ACTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	220	0.12671059300557527	No Hit
ACTTAAGGGTTATTTATTTATTTATTTATTTATTTATTTATTTATTGGATA	195	0.11231166198221443	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	195	0.11231166198221443	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGT	193	0.11115974750034559	No Hit
GCCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTG	188	0.10827996129567341	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGCTG	35	1.2039709E-7	45.000004	6
CGCAGGG	35	1.2039709E-7	45.000004	3
GTATAGG	35	1.2039709E-7	45.000004	2
CGCAAGG	30	2.1548294E-6	45.000004	2
TTTAGTG	25	3.877089E-5	45.0	37
TAATAAC	20	7.0167315E-4	45.0	29
CAGCATT	25	3.877089E-5	45.0	45
ATAGATG	20	7.0167315E-4	45.0	1
GGTTTCA	20	7.0167315E-4	45.0	8
GCGTTGG	25	3.877089E-5	45.0	2
CGTTATT	175	0.0	45.0	1
CGTTAGG	20	7.0167315E-4	45.0	2
CATAGTA	20	7.0167315E-4	45.0	10
CGAGGGC	20	7.0167315E-4	45.0	4
CTCATGC	20	7.0167315E-4	45.0	23
GCGATGC	20	7.0167315E-4	45.0	9
CATGTCT	20	7.0167315E-4	45.0	4
AGCTCTA	20	7.0167315E-4	45.0	10
ACGTAGG	25	3.877089E-5	45.0	2
ATAGTAT	20	7.0167315E-4	45.0	11

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content