##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935161.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	173624
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.239419665484036	33.0	31.0	34.0	31.0	34.0
2	32.366614062572	34.0	31.0	34.0	31.0	34.0
3	32.337528221904805	34.0	31.0	34.0	31.0	34.0
4	36.03361286458093	37.0	35.0	37.0	35.0	37.0
5	33.203750633552964	37.0	35.0	37.0	30.0	37.0
6	34.409499838731975	37.0	35.0	37.0	29.0	37.0
7	35.66782818043588	37.0	35.0	37.0	32.0	37.0
8	35.735491637100864	37.0	35.0	37.0	35.0	37.0
9	37.902870570888815	39.0	38.0	39.0	35.0	39.0
10	37.427567617380085	39.0	37.0	39.0	34.0	39.0
11	37.255765331981756	39.0	37.0	39.0	34.0	39.0
12	37.58207966640557	39.0	37.0	39.0	35.0	39.0
13	37.53834723310141	39.0	37.0	39.0	35.0	39.0
14	38.70299036999493	40.0	38.0	41.0	35.0	41.0
15	38.841294982260514	40.0	38.0	41.0	35.0	41.0
16	38.82812859973276	40.0	38.0	41.0	35.0	41.0
17	38.76230244666636	40.0	38.0	41.0	35.0	41.0
18	38.47030940422983	39.0	38.0	41.0	35.0	41.0
19	38.098863060406394	39.0	37.0	41.0	35.0	41.0
20	37.76067248767451	39.0	35.0	41.0	34.0	41.0
21	37.69117172741096	39.0	35.0	41.0	34.0	41.0
22	37.610088467032206	39.0	35.0	41.0	34.0	41.0
23	37.33299543841865	39.0	35.0	40.0	33.0	41.0
24	37.09899553057181	39.0	35.0	40.0	33.0	41.0
25	36.91345090540478	38.0	35.0	40.0	33.0	41.0
26	37.166791457402205	39.0	35.0	40.0	33.0	41.0
27	37.035957010551535	39.0	35.0	40.0	33.0	41.0
28	37.08305303414274	39.0	35.0	40.0	33.0	41.0
29	37.0824713173294	39.0	35.0	40.0	33.0	41.0
30	36.73754204487859	39.0	35.0	40.0	33.0	41.0
31	36.4972872413952	39.0	35.0	40.0	33.0	41.0
32	36.118998525549465	39.0	35.0	41.0	31.0	41.0
33	35.68124222457725	39.0	35.0	41.0	27.0	41.0
34	35.397473851541264	39.0	35.0	41.0	23.0	41.0
35	35.055810256646545	39.0	35.0	41.0	21.0	41.0
36	34.78006496797678	39.0	35.0	41.0	18.0	41.0
37	34.70425747592499	39.0	35.0	41.0	18.0	41.0
38	34.594808321430214	39.0	35.0	41.0	18.0	41.0
39	34.480210109201494	39.0	35.0	40.0	16.0	41.0
40	34.37433188960052	39.0	35.0	40.0	15.0	41.0
41	34.17924365295121	38.0	35.0	40.0	15.0	41.0
42	34.09272335621804	38.0	35.0	40.0	14.0	41.0
43	33.96958945767866	38.0	35.0	40.0	12.0	41.0
44	33.881012993595355	38.0	34.0	40.0	10.0	41.0
45	33.89042989448463	38.0	34.0	40.0	10.0	41.0
46	33.74199995392342	38.0	34.0	40.0	10.0	41.0
47	33.611856655761876	38.0	34.0	40.0	10.0	41.0
48	33.53060636778326	38.0	34.0	40.0	10.0	41.0
49	33.50540823849237	38.0	34.0	40.0	10.0	41.0
50	33.42427890153435	38.0	34.0	40.0	10.0	41.0
51	32.54146316177487	36.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	5.0
11	8.0
12	6.0
13	6.0
14	10.0
15	16.0
16	17.0
17	41.0
18	71.0
19	111.0
20	233.0
21	433.0
22	729.0
23	1175.0
24	1985.0
25	3135.0
26	4385.0
27	4562.0
28	4057.0
29	3266.0
30	3046.0
31	3156.0
32	3587.0
33	4885.0
34	8414.0
35	14160.0
36	17472.0
37	21146.0
38	36842.0
39	36654.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.760148366585266	22.494586001935215	22.265930977284246	24.479334654195274
2	40.239252637884164	23.56701838455513	22.285513523476016	13.908215454084688
3	23.825047228493755	24.076164585541168	39.27625213104179	12.822536054923283
4	21.852393678293325	24.697622448509424	39.215776620743675	14.23420725245358
5	16.137169976500946	32.63028152789937	36.70057134958301	14.531977146016681
6	19.360226696770034	31.481246832235176	37.77069990323918	11.38782656775561
7	59.391558770676866	9.68068930562595	26.3782656775561	4.549486246141086
8	58.743606874625634	8.649149887112381	26.550476892595494	6.056766345666498
9	53.6129797723817	11.532967792471087	28.616435515827305	6.23761691931991
10	24.738515412615765	27.992673823895313	35.4207943602267	11.848016403262223
11	17.332281251439895	25.89273372344837	39.954153803621615	16.820831221490117
12	18.008455052296917	22.040731696078883	43.837257522001565	16.113555729622632
13	17.493549278901536	24.384877666682026	44.59579320831221	13.525779846104225
14	13.3731511772566	26.955950790213336	43.04934801640326	16.6215500161268
15	11.694811777173662	26.400152052711608	45.854835736994886	16.050200433119848
16	12.361770262175737	27.47373634981339	42.7538819518039	17.410611436206977
17	12.535709348937935	27.328019167856976	40.42067916877851	19.715592314426576
18	12.350251117357047	27.169630926600007	44.448924111873936	16.03119384416901
19	12.802377551490576	27.61426991660139	42.68073538220523	16.902617149702806
20	13.427291157904437	30.40075104824218	41.54034004515505	14.631617748698336
21	13.52232410265862	28.34573561258812	43.16684329355389	14.965096991199372
22	12.019075703819748	27.47373634981339	40.675828226512465	19.831359719854397
23	11.266299589918445	27.845228770216103	40.9759019490393	19.912569690826153
24	13.540754734368521	27.467400820163114	40.269778371653686	18.72206607381468
25	11.469612495968299	29.722849375662353	38.939317145095146	19.868220983274202
26	11.528360134543611	29.90254803483389	41.20801271713588	17.361079113486618
27	13.357024374510438	29.566764963369117	40.67870801271714	16.397502649403307
28	10.322305672026909	28.65444869372898	41.61809427268119	19.40515136156292
29	11.908491913560336	26.056881537114684	41.89109800488412	20.143528544440862
30	15.675828226512465	29.61917707229415	37.717135879832284	16.9878588213611
31	13.228009952541123	30.9196885223241	36.755863244712714	19.096438280422063
32	14.9270838132977	29.688867898447217	34.769386720729855	20.61466156752523
33	16.480440492097866	30.028106713357598	33.42049486246141	20.07095793208312
34	14.6293139197346	27.06365479426807	36.01115053218449	22.295880753812835
35	15.676980140994331	28.62507487444132	34.23086670045616	21.467078284108187
36	16.36812883011565	32.64928811685021	33.84555130627102	17.13703174676312
37	15.360203658480396	29.61917707229415	36.26399576095471	18.756623508270746
38	16.922199695894577	29.687140026724418	31.795719485785373	21.594940791595633
39	19.87052481223794	26.969773763995764	32.238054646823024	20.921646776943277
40	20.58759157720131	25.849536930378285	32.79903699949316	20.763834492927245
41	18.492835091922778	29.535087315117725	31.162742477998435	20.809335114961065
42	17.871377228954522	29.89045293277427	33.537990139612035	18.700179698659174
43	17.87656084412293	28.665391881306736	34.77169054969359	18.686356724876745
44	17.33861678109017	26.521103073307838	32.95684928350919	23.1834308620928
45	19.21220568584988	24.358959590839977	32.8382020918767	23.590632631433444
46	20.35144910841819	26.299935492789018	33.5690918306225	19.779523568170298
47	16.087637653780583	27.895337050177393	36.704027093028614	19.31299820301341
48	16.793761231166197	26.598857300833984	35.5428972953048	21.064484172695018
49	18.81249136064139	24.746578813988847	36.473068239413905	19.96786158595586
50	17.865041699304243	22.936345205731925	35.06658065705202	24.13203243791181
51	17.513131825093307	22.69271529281666	32.39010735842971	27.404045523660326
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3048.0
1	3204.0
2	3360.0
3	7311.0
4	11262.0
5	7191.0
6	3120.0
7	3135.0
8	3150.0
9	3244.5
10	3339.0
11	3560.0
12	3781.0
13	3722.0
14	3663.0
15	3462.0
16	3261.0
17	2863.5
18	2466.0
19	2370.0
20	2274.0
21	2263.0
22	2252.0
23	2279.5
24	2307.0
25	2211.5
26	2218.0
27	2320.0
28	2172.5
29	2025.0
30	2227.5
31	2430.0
32	2662.5
33	2895.0
34	3179.0
35	3463.0
36	3537.0
37	3611.0
38	3834.5
39	4058.0
40	4685.5
41	5313.0
42	6765.0
43	8217.0
44	9119.0
45	10021.0
46	12048.0
47	14075.0
48	16408.0
49	18741.0
50	18237.5
51	17734.0
52	14144.0
53	10554.0
54	8157.0
55	5760.0
56	4595.0
57	3430.0
58	3024.5
59	2619.0
60	2410.0
61	2201.0
62	1917.5
63	1634.0
64	1331.0
65	1028.0
66	879.5
67	731.0
68	616.0
69	501.0
70	406.5
71	312.0
72	255.0
73	198.0
74	185.5
75	130.0
76	87.0
77	63.5
78	40.0
79	36.0
80	32.0
81	22.5
82	13.0
83	10.5
84	8.0
85	4.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	173624.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.54298944846335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.27054117173795	40.85840667188868
2	9.262383926875328	9.548219140211032
3	3.4305125655093813	5.304566189006128
4	2.0929478942016515	4.315071649080772
5	1.3532087025510946	3.487421093857992
6	0.9699299370886458	2.9995853107865273
7	0.7542657920908248	2.721397963415196
8	0.6157043725067325	2.5388195180389808
9	0.43691544401112964	2.02679353084827
>10	1.7152562827546907	13.217642722204303
>50	0.06369355577655854	2.1592636962631895
>100	0.02681833927434044	2.84926047090264
>500	0.003352292409292555	1.2112380776851126
>1k	0.003352292409292555	2.7392526378841637
>5k	0.0011174308030975182	4.023061327927015
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6985	4.023061327927015	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2549	1.4681150071418698	No Hit
CCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	1116	0.6427682808828272	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCC	1091	0.6283693498594665	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	893	0.5143298161544487	No Hit
GCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	688	0.39625858176288986	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	522	0.300649679767774	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	476	0.2741556466847901	No Hit
CTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGCT	400	0.23038289637377318	Illumina Single End Adapter 1 (95% over 21bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	367	0.2113763074229369	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCG	348	0.2004331198451827	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	299	0.17221121503939546	No Hit
TCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	266	0.15320462608855917	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGC	229	0.13189420817398517	No Hit
ACTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	220	0.12671059300557527	No Hit
ACTTAAGGGTTATTTATTTATTTATTTATTTATTTATTTATTTATTGGATA	195	0.11231166198221443	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	195	0.11231166198221443	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGT	193	0.11115974750034559	No Hit
GCCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTG	188	0.10827996129567341	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2442058701561996	0.0
2	0.0	0.0	0.0	1.634566649771921	0.0
3	0.0	0.0	0.0	2.3193798092429616	0.0
4	0.0	0.0	0.0	3.1476063217066765	0.0
5	0.0	0.0	0.0	5.402478919964982	0.0
6	0.0	0.0	0.0	6.67419250794821	0.0
7	0.0	0.0	0.0	7.992558632447127	0.0
8	0.0	0.0	0.0	9.8638437082431	0.0
9	0.0	0.0	0.0	10.641961940745519	0.0
10	0.0	0.0	0.0	12.782219048057872	0.0
11	0.0	0.0	0.0	16.59390406856195	0.0
12	0.0	0.0	0.0	19.077431691471226	0.0
13	0.0	0.0	0.0	20.1291296134175	0.0
14	0.0	0.0	0.0	20.515596922084505	0.0
15	0.0	0.0	0.0	21.307538128369348	0.0
16	0.0	0.0	0.0	22.842464175459614	0.0
17	0.0	0.0	0.0	24.673432244390177	0.0
18	0.0	0.0	0.0	26.48481776712897	0.0
19	0.0	0.0	0.0	27.575680781458786	0.0
20	0.0	0.0	0.0	28.68958208542598	0.0
21	0.0	0.0	0.0	30.010252038888634	0.0
22	0.0	0.0	0.0	31.207091185550386	0.0
23	0.0	0.0	0.0	32.37570842740635	0.0
24	0.0	0.0	0.0	33.108901995115886	0.0
25	0.0	0.0	0.0	33.743030917384694	0.0
26	0.0	0.0	0.0	34.22798691425149	0.0
27	0.0	0.0	0.0	34.68644887803529	0.0
28	0.0	0.0	0.0	35.121296594940794	0.0
29	0.0	0.0	0.0	35.54923282495508	0.0
30	0.0	0.0	0.0	36.05665115421831	0.0
31	0.0	0.0	0.0	36.42699166013915	0.0
32	0.0	0.0	0.0	36.773141961940745	0.0
33	0.0	0.0	0.0	37.10892503340552	0.0
34	0.0	0.0	0.0	37.42858130212413	0.0
35	0.0	0.0	0.0	37.756300972215826	0.0
36	0.0	0.0	0.0	38.0235451320094	0.0
37	0.0	0.0	0.0	38.308643966271944	0.0
38	0.0	0.0	0.0	38.61044556052159	0.0
39	0.0	0.0	0.0	38.876537805833294	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGCTG	35	1.2039709E-7	45.000004	6
CGCAGGG	35	1.2039709E-7	45.000004	3
GTATAGG	35	1.2039709E-7	45.000004	2
CGCAAGG	30	2.1548294E-6	45.000004	2
TTTAGTG	25	3.877089E-5	45.0	37
TAATAAC	20	7.0167315E-4	45.0	29
CAGCATT	25	3.877089E-5	45.0	45
ATAGATG	20	7.0167315E-4	45.0	1
GGTTTCA	20	7.0167315E-4	45.0	8
GCGTTGG	25	3.877089E-5	45.0	2
CGTTATT	175	0.0	45.0	1
CGTTAGG	20	7.0167315E-4	45.0	2
CATAGTA	20	7.0167315E-4	45.0	10
CGAGGGC	20	7.0167315E-4	45.0	4
CTCATGC	20	7.0167315E-4	45.0	23
GCGATGC	20	7.0167315E-4	45.0	9
CATGTCT	20	7.0167315E-4	45.0	4
AGCTCTA	20	7.0167315E-4	45.0	10
ACGTAGG	25	3.877089E-5	45.0	2
ATAGTAT	20	7.0167315E-4	45.0	11
>>END_MODULE