FastQCFastQC Report
Sat 14 Jan 2017
SRR2935159.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935159.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290557
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87092.9973464759066206No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38211.3150603840210355No Hit
CCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC26630.9165155201905306No Hit
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC22940.7895180635813283No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCC19390.667338938659196No Hit
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT16830.5792323020956301No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12420.4274548539529249No Hit
TCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC11690.4023306958703456No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9420.3242048892299962No Hit
ACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC8190.2818724036935954No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCG6370.21923409176168532No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6270.21579242627092102No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5200.1789666055197431No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTG5050.17380410728359666No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.16416744390945665No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGC4470.1538424474371638No Hit
ACCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTG4410.15177744814270522No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.13112745519811947No Hit
GCCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTG3650.1256207904128966No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTC3250.11185412844983945No Hit
TGCATAGGGATTCTTTTAGTTAATATTTGTATTTTAACATGTTGCATAATA3220.11082162880261016No Hit
AGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTG3150.10841246295907517No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGT3070.10565913056646373No Hit
GGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTG2970.10221746507569944No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG253.883587E-545.0000041
ATCTATA351.2077362E-745.00000410
TGCCAGT302.1598844E-645.00000424
GCATTGG351.2077362E-745.0000042
CTTATGG502.1827873E-1145.0000042
AGGCATG253.883587E-545.0000041
GACGCAT302.1598844E-645.00000421
CCGGGCG207.024579E-445.05
CACGGGT207.024579E-445.04
CGAAAGG406.7848305E-945.02
GGGTCAA207.024579E-445.07
CGGTTTG207.024579E-445.010
ACTCTTA406.7848305E-945.05
TTGTCGG207.024579E-445.02
AGGACGC207.024579E-445.014
CGTCTGG207.024579E-445.02
CGACAGG453.8198777E-1045.02
AGAACGG207.024579E-445.02
GACTTTC207.024579E-445.09
TCTACGG207.024579E-445.02
Produced by FastQC (version 0.11.5)