SRR2935158.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935158.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	382621
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9070	2.3704919489520964	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5980	1.5629042838735983	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	3779	0.9876614195248039	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	3695	0.9657075800857768	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCC	2876	0.7516576455552623	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT	2638	0.6894551004780187	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	1877	0.4905637693696896	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1640	0.42862257952386307	No Hit
ACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	1337	0.3494319444045152	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCG	934	0.24410578614346834	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	811	0.21195909267917862	No Hit
ACCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTG	696	0.1819032410662248	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTG	663	0.17327851842946415	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	642	0.16779005856970736	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	625	0.16334701963561854	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGC	623	0.16282430917278456	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTG	581	0.15184738945327098	No Hit
AGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTG	579	0.15132467899043703	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	563	0.14714299528776517	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTC	499	0.13041626047707783	No Hit
AGCAAAGGGTTTTATAATTGTTATCATTTACATGCTTATTTTTAAAATAGT	442	0.11551901228630942	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGT	440	0.11499630182347546	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	438	0.11447359136064147	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	409	0.10689428964954877	No Hit
GGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTG	393	0.10271260594687692	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCTAG	30	2.1616906E-6	45.000004	1
ACGATAG	45	3.8380676E-10	45.000004	1
CCGAGGG	60	0.0	45.000004	3
GTCCGAG	30	2.1616906E-6	45.000004	1
AGACACG	30	2.1616906E-6	45.000004	1
CTACCGG	30	2.1616906E-6	45.000004	2
TGGTATG	30	2.1616906E-6	45.000004	1
TCCGGCA	20	7.0273806E-4	45.0	31
GTGCACG	20	7.0273806E-4	45.0	1
GAGTGTA	20	7.0273806E-4	45.0	9
ATGGGCG	25	3.8859114E-5	45.0	5
AGACTAT	20	7.0273806E-4	45.0	37
ACCGGTA	20	7.0273806E-4	45.0	4
CGAACGG	20	7.0273806E-4	45.0	2
TTGGACG	20	7.0273806E-4	45.0	1
CTCCGGC	20	7.0273806E-4	45.0	30
GATCGAG	35	1.2090823E-7	45.0	1
TTTACGG	25	3.8859114E-5	45.0	2
AGATACG	40	6.7939254E-9	45.0	1
CCGATGA	20	7.0273806E-4	45.0	11

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content