##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935158.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	382621
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47727646940445	31.0	31.0	34.0	30.0	34.0
2	31.58623546538219	31.0	31.0	34.0	30.0	34.0
3	31.60454339934295	31.0	31.0	34.0	30.0	34.0
4	35.44077559778475	37.0	35.0	37.0	33.0	37.0
5	32.882928537639074	35.0	35.0	37.0	28.0	37.0
6	33.92643895656538	36.0	35.0	37.0	26.0	37.0
7	35.236380125502784	37.0	35.0	37.0	32.0	37.0
8	35.19878417546345	37.0	35.0	37.0	32.0	37.0
9	37.22369916967443	39.0	37.0	39.0	34.0	39.0
10	36.686094072201996	39.0	35.0	39.0	32.0	39.0
11	36.58877322467925	39.0	35.0	39.0	32.0	39.0
12	36.98844287166674	39.0	37.0	39.0	33.0	39.0
13	36.953980570852096	39.0	37.0	39.0	33.0	39.0
14	37.853220288483904	39.0	37.0	41.0	33.0	41.0
15	38.00104280737335	39.0	37.0	41.0	33.0	41.0
16	37.94529835006443	40.0	37.0	41.0	33.0	41.0
17	37.911303875114015	39.0	37.0	41.0	33.0	41.0
18	37.81501799430768	39.0	37.0	40.0	33.0	41.0
19	37.583786566863814	39.0	36.0	40.0	33.0	41.0
20	37.41662114729719	39.0	35.0	40.0	33.0	41.0
21	37.320549577780625	39.0	35.0	40.0	33.0	41.0
22	37.257552512799876	39.0	35.0	40.0	33.0	41.0
23	36.996868964327625	39.0	35.0	40.0	32.0	41.0
24	36.698231409149	38.0	35.0	40.0	31.0	41.0
25	36.513706775111665	38.0	35.0	40.0	31.0	41.0
26	36.858923059633426	38.0	35.0	40.0	32.0	41.0
27	36.77064771667002	39.0	35.0	40.0	32.0	41.0
28	36.86844945781857	39.0	35.0	40.0	32.0	41.0
29	37.00412941265639	39.0	35.0	40.0	33.0	41.0
30	36.736279503738686	39.0	35.0	40.0	32.0	41.0
31	36.58014066138555	39.0	35.0	40.0	31.0	41.0
32	36.42658400871881	39.0	35.0	40.0	31.0	41.0
33	36.289218312638354	39.0	35.0	40.0	30.0	41.0
34	36.19727354222586	39.0	35.0	40.0	30.0	41.0
35	35.9828289612959	39.0	35.0	40.0	30.0	41.0
36	35.78016366064591	39.0	35.0	40.0	28.0	41.0
37	35.751095731807716	39.0	35.0	40.0	28.0	41.0
38	35.65272162270236	39.0	35.0	40.0	27.0	41.0
39	35.57578125612552	39.0	35.0	40.0	26.0	41.0
40	35.45027586044676	39.0	35.0	40.0	25.0	41.0
41	35.25514281756621	38.0	35.0	40.0	24.0	41.0
42	35.30620640268046	39.0	35.0	40.0	24.0	41.0
43	35.1803298825731	39.0	35.0	40.0	23.0	41.0
44	35.066663878877534	39.0	35.0	40.0	23.0	41.0
45	35.07919847577629	39.0	35.0	40.0	23.0	41.0
46	34.93115119138782	38.0	35.0	40.0	23.0	41.0
47	34.83166632254895	38.0	35.0	40.0	23.0	41.0
48	34.795152383167675	38.0	35.0	40.0	22.0	41.0
49	34.779533794538196	38.0	35.0	40.0	22.0	41.0
50	34.66683219164656	38.0	34.0	40.0	20.0	41.0
51	33.71007602823682	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	4.0
10	11.0
11	5.0
12	13.0
13	18.0
14	23.0
15	31.0
16	67.0
17	100.0
18	185.0
19	351.0
20	665.0
21	986.0
22	1625.0
23	2364.0
24	3238.0
25	4582.0
26	5771.0
27	6568.0
28	6511.0
29	6636.0
30	7295.0
31	8873.0
32	11049.0
33	14879.0
34	21426.0
35	34687.0
36	43035.0
37	55110.0
38	83235.0
39	63269.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.1737646391599	24.70878493339362	22.992726483909664	18.124723943536818
2	34.54933210670612	25.038876590673276	25.276709851262737	15.135081451357872
3	25.50957736245528	25.14550952509141	35.74686177705876	13.598051335394553
4	23.21121945737427	27.86987645738211	33.756380334586964	15.162523750656657
5	16.990180883955663	35.21552659158802	31.854236960334113	15.940055564122199
6	19.821180750664496	33.52220604723735	33.04575546036418	13.610857741733987
7	63.75212024431487	9.570305864027327	20.574929238071093	6.102644653586708
8	60.477600549891406	8.740764359509802	21.29600832155057	9.485626769048222
9	54.179200827973375	12.143870827790424	24.992616714712472	8.684311629523732
10	22.535877539392768	33.77885688448883	30.609924703557827	13.075340872560576
11	15.632963167207237	26.81034234921763	37.739434061381885	19.817260422193243
12	15.810946079802207	23.70491948952096	42.73262575760348	17.751508673073353
13	17.332033526649084	25.879917725373147	43.02116193308783	13.766886814889931
14	13.31212871222437	29.806257366950582	38.14218247299547	18.739431447829574
15	10.808345595249607	29.301841770315797	42.309491637939374	17.580320996495228
16	11.47140381735451	30.841224083361862	36.60802726457774	21.079344834705886
17	10.716609909022244	31.17680420050128	35.031793863901875	23.0747920265746
18	10.769403665768476	28.91163840981023	42.12549755502181	18.19346036939948
19	11.095313639345463	30.894279195339514	37.84632835103144	20.164078814283588
20	12.138382367930669	32.42294594389749	38.38315199636194	17.055519691809913
21	13.282856926305667	31.783148337388695	38.559566777568406	16.374427958737236
22	10.011734849890622	30.805679771889157	34.70379304847355	24.478792329746668
23	10.197035708965268	29.72758944229407	36.22174423254343	23.85363061619723
24	14.305539946840346	28.597750776878428	34.7675637249393	22.329145551341927
25	11.331317413315004	32.00242537654755	32.0447649240371	24.621492286100345
26	13.379297006698534	30.804895706194902	34.65753317251275	21.158274114593816
27	14.418706762043904	29.41187232274235	34.97664791007289	21.192773005140857
28	10.537581575501607	27.741812393987786	37.5164980489832	24.20410798152741
29	15.126195373489693	25.600006272525555	35.94967343663835	23.32412491734641
30	16.46982261820444	27.55546611398747	31.566223495312595	24.408487772495498
31	13.598312690625972	30.3679097592657	34.148674536943865	21.885103013164464
32	16.560251528274716	28.56952441188539	32.483057647123395	22.3871664127165
33	14.160487793403917	25.430125372104513	33.66490600359102	26.744480830900553
34	15.277258697248714	23.176720566827225	38.13251232943304	23.41350840649102
35	19.530553733328805	23.12523358623808	31.386933806560542	25.957278873872582
36	17.899174378823954	23.713282856926305	34.865833291952086	23.52170947229765
37	14.86614691822979	24.0177617015271	41.60226438172499	19.513826998518116
38	15.420481364065225	25.985505238865613	36.0442840304113	22.549729366657868
39	17.720930110997568	21.688563879138886	38.272860088703965	22.31764592115958
40	21.215249555042718	21.68542761636188	35.88250514216418	21.21681768643122
41	20.929065576641115	23.767906100292457	31.282914424456575	24.02011389860985
42	20.844909192124845	21.03883477383625	36.88009806048283	21.23615797355608
43	20.41890016491515	20.432490636948835	37.41639899535049	21.732210202785524
44	16.85767378162725	21.44236725114408	34.75501867383129	26.944940293397384
45	20.05430961708845	18.606401635038328	33.69783676274956	27.64145198512366
46	22.41931310618079	20.255291790048116	35.76411122233228	21.561283881438815
47	15.669552899605616	21.68307541927913	41.40415711631092	21.243214564804337
48	16.44943691015391	19.681355701856408	39.30416783187541	24.565039556114275
49	19.57158650466127	17.39763368973475	41.781815425708466	21.24896437989551
50	18.77471440407087	16.66505497607293	37.740479482307556	26.819751137548646
51	17.409917385611347	16.781619409284907	32.96630347001341	32.842159735090334
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7499.0
1	6617.5
2	5736.0
3	10030.5
4	14325.0
5	9148.0
6	3971.0
7	4114.5
8	4258.0
9	4783.0
10	5308.0
11	5548.0
12	5788.0
13	5859.5
14	5931.0
15	5399.0
16	4867.0
17	4523.5
18	4180.0
19	4192.5
20	4205.0
21	4049.5
22	3894.0
23	3479.0
24	3064.0
25	3727.5
26	4537.5
27	4684.0
28	4648.5
29	4613.0
30	4741.0
31	4869.0
32	5297.5
33	5726.0
34	5915.5
35	6105.0
36	6425.0
37	6745.0
38	7353.5
39	7962.0
40	9487.0
41	11012.0
42	14768.0
43	18524.0
44	22707.0
45	26890.0
46	34745.5
47	42601.0
48	51165.5
49	59730.0
50	56971.5
51	54213.0
52	39977.5
53	25742.0
54	18442.0
55	11142.0
56	8133.0
57	5124.0
58	4136.5
59	3149.0
60	2708.0
61	2267.0
62	1953.5
63	1640.0
64	1344.0
65	1048.0
66	812.0
67	576.0
68	442.0
69	308.0
70	261.5
71	215.0
72	162.5
73	110.0
74	106.0
75	82.0
76	62.0
77	42.0
78	22.0
79	17.5
80	13.0
81	8.5
82	4.0
83	3.0
84	2.0
85	1.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	382621.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.44334531576949
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.01444227484042	26.590182845036285
2	11.543805903946456	8.644774214393713
3	5.04051482259938	5.662012112155295
4	2.8741624523582288	4.304730287890721
5	1.901033094471834	3.559051930650736
6	1.4109827575086435	3.1699148774395667
7	1.0521366081547845	2.757686003695143
8	0.8486112853270293	2.5419876316287158
9	0.6846540062147564	2.3072162738871698
>10	3.4099814438019864	20.897979451750448
>50	0.12117575800896252	3.034103886726588
>100	0.0850356196554123	6.020712837228947
>500	0.007086301637951024	1.7822541151851765
>1k	0.004960411146565717	4.734104201746899
>5k	0.0014172603275902048	3.993289330584622
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9070	2.3704919489520964	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5980	1.5629042838735983	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	3779	0.9876614195248039	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	3695	0.9657075800857768	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCC	2876	0.7516576455552623	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT	2638	0.6894551004780187	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	1877	0.4905637693696896	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1640	0.42862257952386307	No Hit
ACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	1337	0.3494319444045152	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCG	934	0.24410578614346834	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	811	0.21195909267917862	No Hit
ACCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTG	696	0.1819032410662248	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTG	663	0.17327851842946415	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	642	0.16779005856970736	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	625	0.16334701963561854	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGC	623	0.16282430917278456	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTG	581	0.15184738945327098	No Hit
AGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTG	579	0.15132467899043703	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	563	0.14714299528776517	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTC	499	0.13041626047707783	No Hit
AGCAAAGGGTTTTATAATTGTTATCATTTACATGCTTATTTTTAAAATAGT	442	0.11551901228630942	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGT	440	0.11499630182347546	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	438	0.11447359136064147	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	409	0.10689428964954877	No Hit
GGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTG	393	0.10271260594687692	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6135523141698964E-4	0.0	0.0	0.73101058227332	0.0
2	2.6135523141698964E-4	0.0	0.0	3.691642643764979	0.0
3	2.6135523141698964E-4	0.0	0.0	4.914523771565073	0.0
4	2.6135523141698964E-4	0.0	0.0	6.426986495775193	0.0
5	2.6135523141698964E-4	0.0	0.0	10.226830205346806	0.0
6	2.6135523141698964E-4	0.0	0.0	12.138643723162085	0.0
7	2.6135523141698964E-4	0.0	0.0	14.290119988186744	0.0
8	2.6135523141698964E-4	0.0	0.0	17.50975508401264	0.0
9	2.6135523141698964E-4	0.0	0.0	18.676183481826666	0.0
10	2.6135523141698964E-4	0.0	0.0	21.92456765310843	0.0
11	2.6135523141698964E-4	0.0	0.0	27.21152262944271	0.0
12	2.6135523141698964E-4	0.0	0.0	30.85455320016413	0.0
13	2.6135523141698964E-4	0.0	0.0	32.20288483904438	0.0
14	2.6135523141698964E-4	0.0	0.0	32.719322776324354	0.0
15	2.6135523141698964E-4	0.0	0.0	33.79584497453093	0.0
16	2.6135523141698964E-4	0.0	0.0	36.051340621659556	0.0
17	2.6135523141698964E-4	0.0	0.0	38.90847601151008	0.0
18	2.6135523141698964E-4	0.0	0.0	41.73895316775608	0.0
19	2.6135523141698964E-4	0.0	0.0	43.50937350537477	0.0
20	2.6135523141698964E-4	0.0	0.0	45.14127557034245	0.0
21	5.227104628339793E-4	0.0	0.0	47.04237352366964	0.0
22	5.227104628339793E-4	0.0	0.0	48.88283706330808	0.0
23	5.227104628339793E-4	0.0	0.0	50.517091325358514	0.0
24	5.227104628339793E-4	0.0	0.0	51.71200744339699	0.0
25	5.227104628339793E-4	0.0	0.0	52.56036652457654	0.0
26	5.227104628339793E-4	0.0	0.0	53.27517308250201	0.0
27	5.227104628339793E-4	0.0	0.0	53.90948222915104	0.0
28	5.227104628339793E-4	0.0	0.0	54.501974538773354	0.0
29	5.227104628339793E-4	0.0	0.0	55.19822487526822	0.0
30	5.227104628339793E-4	0.0	0.0	55.82704556205749	0.0
31	5.227104628339793E-4	0.0	0.0	56.3455743411888	0.0
32	5.227104628339793E-4	0.0	0.0	56.787264682283514	0.0
33	5.227104628339793E-4	0.0	0.0	57.19367206713693	0.0
34	5.227104628339793E-4	0.0	0.0	57.580477809634075	0.0
35	5.227104628339793E-4	0.0	0.0	57.960749671345795	0.0
36	5.227104628339793E-4	0.0	0.0	58.29502301232812	0.0
37	5.227104628339793E-4	0.0	0.0	58.64288682534414	0.0
38	5.227104628339793E-4	0.0	0.0	58.98761437558315	0.0
39	5.227104628339793E-4	0.0	0.0	59.336262254293416	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCTAG	30	2.1616906E-6	45.000004	1
ACGATAG	45	3.8380676E-10	45.000004	1
CCGAGGG	60	0.0	45.000004	3
GTCCGAG	30	2.1616906E-6	45.000004	1
AGACACG	30	2.1616906E-6	45.000004	1
CTACCGG	30	2.1616906E-6	45.000004	2
TGGTATG	30	2.1616906E-6	45.000004	1
TCCGGCA	20	7.0273806E-4	45.0	31
GTGCACG	20	7.0273806E-4	45.0	1
GAGTGTA	20	7.0273806E-4	45.0	9
ATGGGCG	25	3.8859114E-5	45.0	5
AGACTAT	20	7.0273806E-4	45.0	37
ACCGGTA	20	7.0273806E-4	45.0	4
CGAACGG	20	7.0273806E-4	45.0	2
TTGGACG	20	7.0273806E-4	45.0	1
CTCCGGC	20	7.0273806E-4	45.0	30
GATCGAG	35	1.2090823E-7	45.0	1
TTTACGG	25	3.8859114E-5	45.0	2
AGATACG	40	6.7939254E-9	45.0	1
CCGATGA	20	7.0273806E-4	45.0	11
>>END_MODULE