FastQCFastQC Report
Sat 14 Jan 2017
SRR2935157.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935157.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277123
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82302.969800413534784No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40021.4441240893033058No Hit
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC21150.7631990127127665Illumina PCR Primer Index 7 (96% over 27bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC20800.7505692418168106No Hit
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC16400.591794979124793Illumina PCR Primer Index 7 (96% over 27bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14270.5149338019579753No Hit
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT12880.46477556897117883Illumina PCR Primer Index 7 (96% over 28bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA10010.3612114476243401No Hit
TCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC8300.2995059955326696Illumina PCR Primer Index 7 (96% over 27bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7770.280380913890222No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7680.27713325851697623No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG7250.2616166828448018No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6460.23310948567964404No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6420.23166608329153482No Hit
ACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC6220.22444907135098854Illumina PCR Primer Index 7 (96% over 27bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5700.2056848403055683No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC4720.17032148179689163No Hit
CGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG4160.1501138483633621Illumina PCR Primer Index 7 (96% over 26bp)
AGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG3190.11511134045171277Illumina PCR Primer Index 7 (96% over 26bp)
ACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG3160.11402878866063083Illumina PCR Primer Index 7 (96% over 26bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.10500752373494802No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGT2890.1042858225408934No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTC2820.10175986836170221Illumina PCR Primer Index 7 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGG207.0240116E-445.02
TACCCGT207.0240116E-445.011
ATTAGAA207.0240116E-445.029
AATCAGA207.0240116E-445.024
GGGTATC253.8831196E-545.07
AACTTAC207.0240116E-445.024
GGACTAA406.7830115E-945.08
GAATCTA253.8831196E-545.08
ATAGCGG253.8831196E-545.02
GAGAGTC207.0240116E-445.09
ATAGACG207.0240116E-445.01
AGGGTAT253.8831196E-545.06
TGCGGGC253.8831196E-545.04
GCGTTAG253.8831196E-545.01
CCGTCCA207.0240116E-445.014
CAGGGCG253.8831196E-545.05
CGGGGCG207.0240116E-445.06
AGACGGT351.2074634E-745.03
CCCGTCC207.0240116E-445.013
CTACGAG207.0240116E-445.01
Produced by FastQC (version 0.11.5)