SRR2935156.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935156.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1317683
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22845	1.7337250309824137	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	11657	0.884658905062902	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	10648	0.8080851008930069	No Hit
CTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT	9486	0.7199000062989352	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCC	7976	0.605304917798894	No Hit
TCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	6651	0.5047496249097848	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6422	0.4873706346670633	No Hit
ACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	4353	0.3303525961858808	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCG	2799	0.21241831305405015	No Hit
ACCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	2532	0.19215547290205612	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	2434	0.18471817576761634	No Hit
AGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	2367	0.1796334930328463	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTC	2250	0.17075427094377024	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCT	2082	0.15800461871330204	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	1995	0.15140212023680963	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1938	0.14707634537290076	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGC	1863	0.14138453634144177	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGT	1755	0.13318833133614078	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC	1712	0.12992502749143764	No Hit
GGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	1384	0.10503284932719022	No Hit
TGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	1370	0.10397037830798454	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCT	1344	0.10199721784374544	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGT	20	7.033664E-4	45.0	45
GCGACGG	50	2.1827873E-11	45.0	2
CAACGTC	20	7.033664E-4	45.0	27
CGACGGT	20	7.033664E-4	45.0	28
ACGTTAG	70	0.0	45.0	1
TCACACG	25	3.8911185E-5	45.0	1
GCGCGAG	45	3.8562575E-10	45.0	1
ACCGCGC	20	7.033664E-4	45.0	20
GACGTAC	20	7.033664E-4	45.0	10
GAGTACG	35	1.2121018E-7	45.0	1
GGATCGC	40	6.8157533E-9	45.0	8
TTGTACG	35	1.2121018E-7	45.0	1
ACTTACG	35	1.2121018E-7	45.0	1
CCGTCGG	25	3.8911185E-5	45.0	2
CTACGAG	25	3.8911185E-5	45.0	1
TGCGACG	20	7.033664E-4	45.0	1
CGGGCTC	25	3.8911185E-5	45.0	6
ACATGCG	35	1.2121018E-7	45.0	1
ACGTACC	20	7.033664E-4	45.0	11
ACTCCCG	20	7.033664E-4	45.0	10

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content