##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935153.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	943799
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.031810798697606	33.0	31.0	34.0	30.0	34.0
2	32.34322986144296	34.0	31.0	34.0	30.0	34.0
3	32.395168886595556	34.0	31.0	34.0	30.0	34.0
4	35.906225795958676	37.0	35.0	37.0	35.0	37.0
5	30.41287710624826	37.0	35.0	37.0	0.0	37.0
6	32.884751944005025	37.0	35.0	37.0	17.0	37.0
7	35.05498734370348	37.0	35.0	37.0	32.0	37.0
8	35.619268509502554	37.0	35.0	37.0	35.0	37.0
9	37.492054982045964	39.0	37.0	39.0	35.0	39.0
10	37.43148700093982	39.0	37.0	39.0	35.0	39.0
11	37.31319274548924	39.0	37.0	39.0	34.0	39.0
12	37.15403385678518	39.0	37.0	39.0	34.0	39.0
13	37.10881766138765	39.0	37.0	39.0	33.0	39.0
14	38.283435350111624	40.0	38.0	41.0	34.0	41.0
15	38.39117545155271	40.0	38.0	41.0	34.0	41.0
16	38.468224696148226	40.0	38.0	41.0	34.0	41.0
17	38.45162794196646	40.0	38.0	41.0	34.0	41.0
18	38.41077814237989	40.0	37.0	41.0	34.0	41.0
19	38.39448124017932	40.0	37.0	41.0	34.0	41.0
20	38.31939109916412	40.0	37.0	41.0	34.0	41.0
21	38.284708926370975	40.0	37.0	41.0	34.0	41.0
22	38.23938889530504	40.0	37.0	41.0	34.0	41.0
23	38.171773862866985	40.0	37.0	41.0	34.0	41.0
24	38.1741885719311	40.0	37.0	41.0	34.0	41.0
25	38.1163595214659	40.0	36.0	41.0	34.0	41.0
26	38.03586462795574	40.0	36.0	41.0	34.0	41.0
27	37.99632336970054	40.0	36.0	41.0	34.0	41.0
28	37.924159699258	40.0	36.0	41.0	34.0	41.0
29	37.89070236353292	40.0	36.0	41.0	34.0	41.0
30	37.751310395539726	40.0	36.0	41.0	33.0	41.0
31	33.161013097068334	38.0	33.0	41.0	9.0	41.0
32	34.02142511276236	38.0	33.0	41.0	16.0	41.0
33	35.93602239459885	38.0	34.0	41.0	29.0	41.0
34	36.75341889533682	38.0	35.0	41.0	31.0	41.0
35	36.87962585253852	39.0	35.0	40.0	31.0	41.0
36	37.048309014949155	39.0	35.0	41.0	32.0	41.0
37	37.14344897589423	39.0	35.0	41.0	33.0	41.0
38	37.08824866311577	39.0	35.0	41.0	32.0	41.0
39	37.14997367024123	39.0	35.0	41.0	33.0	41.0
40	37.12437288024251	39.0	35.0	41.0	33.0	41.0
41	37.05557539264186	39.0	35.0	41.0	32.0	41.0
42	36.97557530787805	39.0	35.0	41.0	32.0	41.0
43	36.98561028354554	39.0	35.0	41.0	32.0	41.0
44	36.93255343563619	39.0	35.0	41.0	32.0	41.0
45	36.92658712289375	39.0	35.0	41.0	32.0	41.0
46	36.87320499385992	39.0	35.0	41.0	32.0	41.0
47	36.78464694283423	39.0	35.0	41.0	32.0	41.0
48	36.76679780334584	39.0	35.0	41.0	32.0	41.0
49	36.74649369198315	39.0	35.0	41.0	32.0	41.0
50	36.57302137425447	39.0	35.0	41.0	31.0	41.0
51	35.90159027504797	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	7.0
12	7.0
13	16.0
14	24.0
15	28.0
16	60.0
17	81.0
18	194.0
19	334.0
20	595.0
21	1027.0
22	1641.0
23	2689.0
24	3758.0
25	4822.0
26	5643.0
27	6639.0
28	8042.0
29	10307.0
30	13723.0
31	19406.0
32	29970.0
33	35141.0
34	62932.0
35	92342.0
36	109208.0
37	110709.0
38	167874.0
39	256335.0
40	241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.36929685240183	22.38241405214458	23.376057825871822	11.872231269581764
2	32.2612123979788	24.854126779112924	25.06900303984217	17.815657783066097
3	32.68651481936302	24.068048387421474	26.06338849691513	17.18204829630038
4	28.94122583304284	25.851161105277715	25.620391629997492	19.587221431681957
5	21.800086670996684	39.206970975811586	20.340665756162064	18.652276597029662
6	26.9671826310475	32.75093531567633	24.82297607859301	15.458905974683168
7	77.09819569632941	7.326877862765271	9.317555962657304	6.257370478248017
8	77.4852484480276	5.722192966934697	9.737136826803166	7.05542175823454
9	71.77418073127859	7.652158987241988	12.37477471368374	8.198885567795685
10	39.96528921942066	25.661713987830037	19.138291097998618	15.234705694750684
11	28.44948977483553	25.917700696864483	25.160653910419484	20.472155617880503
12	25.214902749420165	23.862708055422818	29.918022799346044	21.004366395810973
13	23.27550675514596	26.0591503063682	30.102066223846393	20.563276714639454
14	19.43220961242807	28.691384500301442	29.71405987927514	22.162346007995346
15	18.900316698788618	28.321920239373	31.915376049349494	20.862387012488888
16	21.06168792295817	28.488798992158287	29.29702192945744	21.152491155426105
17	20.842149652627306	27.902339375227143	27.303906870000922	23.95160410214463
18	21.443125072181683	27.464746201256833	29.45288138682071	21.639247339740773
19	22.03488242729649	28.75824195617923	27.357943799474256	21.84893181705003
20	22.823291823788754	28.992084119605977	27.770955468272373	20.413668588332897
21	23.114773378653716	27.88676402496718	29.066358408940886	19.932104187438217
22	21.37436043055778	25.60576987261059	28.64900259483216	24.370867101999472
23	20.698474993086453	26.62950479922102	28.847985641010425	23.8240345666821
24	21.931682487478795	26.446944741412103	28.75697049901515	22.864402272093955
25	20.08732791621945	29.68089603824543	26.35412836843438	23.87764767710074
26	19.33240022504792	29.71522538167555	27.439740877029962	23.512633516246574
27	21.478619918012203	29.06349763032171	27.691277485990128	21.766604965675953
28	18.819367259342297	28.500030197107645	29.201238823096865	23.47936372045319
29	21.58097221972051	28.07123126852222	28.052053456297372	22.2957430554599
30	23.85285426240121	28.54590860977814	26.36165115665518	21.23958597116547
31	25.356034494632862	29.422154505355486	24.772223746793546	20.44958725321811
32	25.08955826399477	28.166908420119114	25.40731660025069	21.336216715635427
33	25.32255278931213	27.58383935562551	26.052475156256786	21.041132698805573
34	22.717125150588206	26.44026959130069	29.08913868313062	21.75346657498048
35	22.934968144700303	27.27042516468019	27.798821571118427	21.99578511950108
36	25.579705000746984	27.26205473835001	27.44397906757689	19.71426119332612
37	25.236199656918473	25.866100726955633	29.796704594940238	19.100995021185653
38	22.79839245432555	27.755909891830782	27.854447822046858	21.591249831796812
39	25.4847695324958	26.079917440048145	27.23302313310355	21.202289894352504
40	24.49080789447753	25.463684534524827	28.597720489214336	21.447787081783304
41	22.102905385574683	26.2311148878098	27.467606979876013	24.19837274673951
42	23.789493313724638	24.39428310477125	28.902128525247434	22.91409505625668
43	24.879661877158167	23.74700545349169	29.061590444575593	22.311742224774555
44	22.524817254521352	25.01104578411293	27.13649834339727	25.327638617968447
45	23.35571451124657	24.491231713532223	26.993777276729475	25.159276498491735
46	24.127594964605812	25.666270042667982	28.165954827246054	22.04018016548015
47	21.388134549835293	25.077373466172354	31.268734126651964	22.265757857340386
48	21.524392375919025	23.038909767863707	31.4445130795858	23.992184776631465
49	23.230687890112193	22.19995994909933	31.949387528488586	22.619964632299887
50	22.25155991900818	22.55967637176984	30.46930543473769	24.71945827448429
51	21.363023270844746	22.817676221314073	28.137982769636334	27.681317738204854
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1469.0
1	1664.0
2	1859.0
3	2169.5
4	2480.0
5	2133.0
6	1786.0
7	1893.0
8	2000.0
9	2246.5
10	2493.0
11	2539.0
12	2585.0
13	2597.5
14	2610.0
15	2734.5
16	2859.0
17	2777.5
18	2696.0
19	3102.5
20	3509.0
21	3717.5
22	3926.0
23	4190.0
24	4454.0
25	5023.0
26	6329.0
27	7066.0
28	9001.5
29	10937.0
30	12593.0
31	14249.0
32	15808.0
33	17367.0
34	19049.0
35	20731.0
36	23395.0
37	26059.0
38	28781.5
39	31504.0
40	35473.0
41	39442.0
42	46587.0
43	53732.0
44	61190.0
45	68648.0
46	78280.5
47	87913.0
48	100151.5
49	112390.0
50	110746.5
51	109103.0
52	93349.5
53	77596.0
54	66567.0
55	55538.0
56	47842.5
57	40147.0
58	38744.0
59	37341.0
60	31992.0
61	26643.0
62	25035.5
63	23428.0
64	19481.0
65	15534.0
66	12782.0
67	10030.0
68	8298.5
69	6567.0
70	5518.5
71	4470.0
72	3695.0
73	2920.0
74	2222.0
75	1406.5
76	1289.0
77	1006.5
78	724.0
79	558.0
80	392.0
81	254.5
82	117.0
83	65.5
84	14.0
85	15.5
86	17.0
87	24.5
88	32.0
89	24.5
90	17.0
91	8.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	943799.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.293794120344852
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.69284333587396	16.869150486380093
2	13.97795790577987	7.071111789832854
3	6.081035962682728	4.6143741503552995
4	3.1969090546233128	3.2344783779643347
5	1.9262974944231255	2.4361686119237334
6	1.1851486489345455	1.7986143556893153
7	0.7945315776608813	1.4067702703226983
8	0.5650330879723389	1.143346447868403
9	0.43088757553004753	0.9808903462024408
>10	3.264764003779508	19.298009258518558
>50	1.6312732150462572	29.15924655312313
>100	0.24423692143650325	8.583587957024108
>500	0.005257546254014747	0.9780316695866964
>1k	0.003345711252554839	1.7766567881639173
>5k	4.77958750364977E-4	0.6495629370444153
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGCT	5373	0.5692949452160895	TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	4273	0.45274470517557236	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	4143	0.4389705858980567	No Hit
TCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	1445	0.1531046335077702	No Hit
ACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	1327	0.1406019713943329	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCC	1320	0.13986028804862052	No Hit
GCCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTG	1101	0.1166561948041903	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1087	0.11517282811276552	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5954657718433692	0.0
2	0.0	0.0	0.0	1.8414937926401702	0.0
3	0.0	0.0	0.0	2.5672839238015723	0.0
4	0.0	0.0	0.0	3.3517729940379253	0.0
5	0.0	0.0	0.0	5.281315195290523	0.0
6	0.0	0.0	0.0	6.501808118042083	0.0
7	0.0	0.0	0.0	7.559660478555285	0.0
8	0.0	0.0	0.0	9.191363839122525	0.0
9	1.0595476367319736E-4	0.0	0.0	9.807278880354822	0.0
10	1.0595476367319736E-4	0.0	0.0	10.977125426070593	0.0
11	1.0595476367319736E-4	0.0	0.0	12.991113573970729	0.0
12	1.0595476367319736E-4	0.0	0.0	14.511458477917438	0.0
13	1.0595476367319736E-4	0.0	0.0	15.144008417046427	0.0
14	1.0595476367319736E-4	0.0	0.0	15.441105574386071	0.0
15	1.0595476367319736E-4	0.0	0.0	15.963462559294934	0.0
16	1.0595476367319736E-4	0.0	0.0	16.99090590263393	0.0
17	1.0595476367319736E-4	0.0	0.0	18.16202390551378	0.0
18	1.0595476367319736E-4	0.0	0.0	19.383046602083706	0.0
19	1.0595476367319736E-4	0.0	0.0	20.184382479744098	0.0
20	1.0595476367319736E-4	0.0	0.0	20.883472010459855	0.0
21	1.0595476367319736E-4	0.0	0.0	21.67135163313375	0.0
22	1.0595476367319736E-4	0.0	0.0	22.519201652046675	0.0
23	1.0595476367319736E-4	0.0	0.0	23.274023388454534	0.0
24	1.0595476367319736E-4	0.0	0.0	23.911765111003508	0.0
25	1.0595476367319736E-4	0.0	0.0	24.484238699129794	0.0
26	1.0595476367319736E-4	0.0	0.0	25.00638377451131	0.0
27	1.0595476367319736E-4	0.0	0.0	25.509668901958996	0.0
28	1.0595476367319736E-4	0.0	0.0	26.026940058211547	0.0
29	1.0595476367319736E-4	0.0	0.0	26.6204986443088	0.0
30	1.0595476367319736E-4	0.0	0.0	27.213633411351356	0.0
31	1.0595476367319736E-4	0.0	0.0	27.758982579977303	0.0
32	3.178642910195921E-4	0.0	0.0	28.35402453276598	0.0
33	3.178642910195921E-4	0.0	0.0	28.94154369733386	0.0
34	3.178642910195921E-4	0.0	0.0	29.51179223542301	0.0
35	3.178642910195921E-4	0.0	0.0	30.08776233075051	0.0
36	3.178642910195921E-4	0.0	0.0	30.65970614505843	0.0
37	3.178642910195921E-4	0.0	0.0	31.213637649541905	0.0
38	3.178642910195921E-4	0.0	0.0	31.858584296020656	0.0
39	3.178642910195921E-4	0.0	0.0	32.49314737565943	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	20	7.032643E-4	45.000004	13
ATCTCGC	20	7.032643E-4	45.000004	36
TCCGCGA	20	7.032643E-4	45.000004	31
GTTTCGA	20	7.032643E-4	45.000004	13
CCTAGTA	20	7.032643E-4	45.000004	19
TCCGATT	20	7.032643E-4	45.000004	42
CTATCGC	20	7.032643E-4	45.000004	30
TTTCGCG	20	7.032643E-4	45.000004	43
TGACGTA	30	2.1650867E-6	45.000004	26
ATGTACG	35	1.2116107E-7	45.000004	1
CCGTAAC	20	7.032643E-4	45.000004	15
CGGATAT	20	7.032643E-4	45.000004	10
TGCGACG	20	7.032643E-4	45.000004	1
AGCGCTA	30	2.1650867E-6	45.000004	22
TCTCGCA	20	7.032643E-4	45.000004	37
AACGCGT	30	2.1650867E-6	45.000004	37
GTCCGCA	20	7.032643E-4	45.000004	2
TCCGTAC	20	7.032643E-4	45.000004	32
CGTAACG	20	7.032643E-4	45.000004	14
ACCAACG	20	7.032643E-4	45.000004	29
>>END_MODULE