##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935152.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	475325
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99184978698785	33.0	31.0	34.0	30.0	34.0
2	32.30488402671856	34.0	31.0	34.0	30.0	34.0
3	32.3560511229159	34.0	31.0	34.0	30.0	34.0
4	35.88585283753221	37.0	35.0	37.0	35.0	37.0
5	30.398998579919002	37.0	35.0	37.0	0.0	37.0
6	32.855711355388415	37.0	35.0	37.0	17.0	37.0
7	35.0039509809078	37.0	35.0	37.0	32.0	37.0
8	35.52223426076895	37.0	35.0	37.0	33.0	37.0
9	37.33610687424394	39.0	37.0	39.0	34.0	39.0
10	37.36619786461895	39.0	37.0	39.0	34.0	39.0
11	37.266503970967236	39.0	37.0	39.0	34.0	39.0
12	37.02606847946142	39.0	37.0	39.0	34.0	39.0
13	36.88762636091096	39.0	35.0	39.0	33.0	39.0
14	38.027198232788095	40.0	37.0	41.0	33.0	41.0
15	38.18092883816336	40.0	37.0	41.0	33.0	41.0
16	38.27237153526534	40.0	37.0	41.0	34.0	41.0
17	38.255269552411505	40.0	37.0	41.0	34.0	41.0
18	38.19443749013833	40.0	37.0	41.0	34.0	41.0
19	38.1392899595014	40.0	36.0	41.0	34.0	41.0
20	38.010009993162576	40.0	36.0	41.0	34.0	41.0
21	37.96820070478094	40.0	36.0	41.0	34.0	41.0
22	37.95072213748488	40.0	36.0	41.0	34.0	41.0
23	37.89802976910535	40.0	35.0	41.0	34.0	41.0
24	37.86702992689213	40.0	35.0	41.0	34.0	41.0
25	37.79235049702835	40.0	35.0	41.0	33.0	41.0
26	37.70081312785989	40.0	35.0	41.0	33.0	41.0
27	37.633473938883924	40.0	35.0	41.0	33.0	41.0
28	37.59005943301951	40.0	35.0	41.0	33.0	41.0
29	37.5758207542208	40.0	35.0	41.0	33.0	41.0
30	37.41742386787987	39.0	35.0	41.0	33.0	41.0
31	32.83835691369063	38.0	33.0	41.0	9.0	41.0
32	33.66579287855678	38.0	33.0	40.0	16.0	41.0
33	35.534575290590645	38.0	34.0	40.0	27.0	41.0
34	36.3975301109767	38.0	35.0	40.0	31.0	41.0
35	36.514502708673014	39.0	35.0	40.0	31.0	41.0
36	36.64340398674591	39.0	35.0	41.0	31.0	41.0
37	36.73576395098091	39.0	35.0	41.0	31.0	41.0
38	36.709806974175564	39.0	35.0	41.0	31.0	41.0
39	36.68570136222584	39.0	35.0	41.0	31.0	41.0
40	36.664862988481566	39.0	35.0	41.0	31.0	41.0
41	36.58495345290065	39.0	35.0	41.0	31.0	41.0
42	36.4647009940567	39.0	35.0	41.0	31.0	41.0
43	36.43058959659181	39.0	35.0	41.0	31.0	41.0
44	36.34247304475885	39.0	35.0	41.0	31.0	41.0
45	36.33534739388839	39.0	35.0	41.0	31.0	41.0
46	36.28402040708988	39.0	35.0	41.0	31.0	41.0
47	36.207237153526535	39.0	35.0	41.0	31.0	41.0
48	36.177863567033086	39.0	35.0	41.0	31.0	41.0
49	36.1491484773576	39.0	35.0	41.0	31.0	41.0
50	35.97080734234471	38.0	35.0	40.0	30.0	41.0
51	35.35815494661547	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	8.0
11	7.0
12	12.0
13	10.0
14	16.0
15	29.0
16	45.0
17	113.0
18	208.0
19	335.0
20	636.0
21	964.0
22	1451.0
23	2263.0
24	2923.0
25	3486.0
26	3776.0
27	4225.0
28	4872.0
29	5719.0
30	7184.0
31	10276.0
32	16503.0
33	18109.0
34	33595.0
35	51998.0
36	52620.0
37	52887.0
38	78558.0
39	122416.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.38831325934887	21.86987850418135	24.523431336454003	12.218376900015778
2	32.71193393993583	23.98927049913217	25.035502024930313	18.263293536001683
3	31.868090254036712	26.727186661757745	24.22489875348446	17.179824330721086
4	28.75674538473676	27.248093409772263	25.236837952979542	18.75832325251144
5	20.46641771419555	40.94314416451901	20.893493925209068	17.69694419607637
6	26.156629674433283	34.15768158628307	24.150633776889496	15.53505496239415
7	74.7574817230316	8.968600431283859	10.048282753905223	6.225635091779309
8	74.06279913743228	8.276232051754064	10.506285173302478	7.154683637511177
9	68.2533003734287	10.066586020091517	12.890548572029664	8.789565034450114
10	39.37916162625572	24.925261663072636	19.954347025719244	15.741229684952401
11	31.22137484878767	25.73312996370904	23.7525903329301	19.292904854573187
12	27.604060379740176	23.638352706043232	28.16998895492558	20.587597959291013
13	22.64282333140483	28.630095198022403	29.997790985115447	18.72929048545732
14	18.47935622994793	31.31036659127965	29.25535160153579	20.954925577236626
15	18.034397517488035	27.295429443012676	34.49934255509388	20.170830484405407
16	20.224477988744543	27.068637248198602	31.74606847946142	20.960816283595435
17	19.71619418292747	27.558197023089466	25.587334981328564	27.1382738126545
18	21.648977015726082	27.227686319886395	30.742544574764636	20.38079208962289
19	22.59170041550518	28.705412086467152	26.803345079682323	21.89954241834534
20	23.450691632041234	29.396518171777203	27.15552516699101	19.997265029190554
21	21.302687634776206	29.294693104717823	29.188870772629254	20.213748487876714
22	20.013464471677274	28.07636880029454	27.13595960658497	24.774207121443222
23	19.98148635144375	27.72818597801504	28.412980592226372	23.877347078314838
24	21.769315731341713	26.271708830799977	29.23662757060958	22.722347867248725
25	20.779045915952242	29.001209698627257	26.813653815810234	23.406090569610267
26	19.4227107768369	30.838689317835165	27.269552411507913	22.469047493820018
27	22.81912375742913	27.85693998842897	28.57202966391416	20.75190659022774
28	20.11066112659759	27.46057960342923	28.805554094566876	23.6232051754063
29	19.646978383211486	26.091831904486405	30.349129542944304	23.912060169357808
30	22.668910745279547	28.864461158154946	27.161626255719774	21.305001840845737
31	25.754588965444697	28.029663914163994	24.676800084152948	21.538947036238362
32	22.49955293746384	29.247777836217324	24.4948193341398	23.757849892179035
33	21.677799400410247	30.617577447009943	23.96528690895703	23.739336243622784
34	20.76473991479514	27.00552253721138	28.4962920107295	23.73344553726398
35	19.55798664071951	26.580129385157527	27.968863409246303	25.89302056487666
36	24.08646715405249	27.357913006890023	27.472571398516806	21.083048440540683
37	22.264135065481515	27.714090359227896	29.269447220322935	20.752327354967655
38	20.58696681218114	28.99447746278862	24.771682533003734	25.646873192026508
39	21.599537158786095	28.088781360122024	27.6800084152948	22.631673065797088
40	22.861200231420607	25.381370641140272	29.72176931573134	22.035659811707777
41	20.382895913322464	26.00305054436438	26.75095986956293	26.86309367275022
42	23.199915847052015	25.062010203544943	27.892073844211858	23.846000105191184
43	24.0	24.333456056382474	27.727344448535213	23.93919949508231
44	21.255141219165836	25.21369589228423	27.83169410403408	25.699468784515854
45	20.50681112922737	24.732130647451743	27.842633987271864	26.91842423604902
46	22.676274128228055	25.165307947194027	28.787461210750536	23.370956713827383
47	20.549308367958766	24.311786672276863	31.148792931152368	23.990112028612
48	19.085467837795193	23.646347236101615	31.285751853994636	25.982433072108556
49	21.858097091463733	22.023668016620206	31.733235154894018	24.384999737022035
50	20.25224846157892	23.10524378057119	30.239099563456584	26.403408194393307
51	19.78898648293273	24.036396150002627	27.368221743017934	28.806395624046704
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1513.0
1	1570.5
2	1628.0
3	1655.5
4	1683.0
5	1578.0
6	1473.0
7	1474.0
8	1475.0
9	1607.0
10	1739.0
11	1799.0
12	1859.0
13	2082.5
14	2306.0
15	2332.0
16	2358.0
17	2432.5
18	2507.0
19	2421.0
20	2335.0
21	2302.5
22	2270.0
23	2164.0
24	2058.0
25	2597.0
26	3124.5
27	3113.0
28	3817.5
29	4522.0
30	5065.0
31	5608.0
32	6839.5
33	8071.0
34	8955.5
35	9840.0
36	10447.5
37	11055.0
38	13364.0
39	15673.0
40	17053.0
41	18433.0
42	23059.0
43	27685.0
44	32347.5
45	37010.0
46	41599.0
47	46188.0
48	50571.5
49	54955.0
50	54128.5
51	53302.0
52	44966.0
53	36630.0
54	31941.5
55	27253.0
56	24459.0
57	21665.0
58	19641.5
59	17618.0
60	15794.5
61	13971.0
62	12333.0
63	10695.0
64	9153.5
65	7612.0
66	6783.5
67	5955.0
68	4788.5
69	3622.0
70	3003.0
71	2384.0
72	2035.5
73	1687.0
74	1412.0
75	900.5
76	664.0
77	550.0
78	436.0
79	262.5
80	89.0
81	70.0
82	51.0
83	49.0
84	47.0
85	26.0
86	5.0
87	4.5
88	4.0
89	4.5
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	475325.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.643321845737354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.86936707766722	21.60090100978467
2	11.395016466794186	6.755722811253138
3	4.815161975470093	4.282121885346493
4	2.5768660248332607	3.0554747570991263
5	1.5605278432888703	2.3129614553923177
6	0.9985276244770167	1.7759805446539063
7	0.6745888348528879	1.399793776155958
8	0.4735264684989466	1.1229518006551746
9	0.35262644721040853	0.9407717339379346
>10	2.8117199271373976	19.786867781420092
>50	1.291142043140086	26.722236256226072
>100	0.1732301489007271	7.269728161091582
>500	0.004619470637352723	0.9199847492343004
>1k	0.003079647091568482	2.0545032777492365
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGCT	3204	0.6740651133435018	No Hit
CCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC	2488	0.5234313364540052	No Hit
GCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC	2175	0.45758165465733974	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1135	0.23878398990164626	No Hit
TCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC	761	0.16010098353757954	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCC	749	0.15757639509809077	No Hit
TGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGCTT	700	0.1472676589701783	Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC	667	0.1403250407615842	No Hit
AAAACTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTC	648	0.13632777573239363	No Hit
CGCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTG	506	0.10645347919844315	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7033082627675801	0.0
2	0.0	0.0	0.0	2.0470204596854784	0.0
3	0.0	0.0	0.0	2.738968074475359	0.0
4	0.0	0.0	0.0	3.5291642560353442	0.0
5	0.0	0.0	0.0	5.453742176405617	0.0
6	0.0	0.0	0.0	6.69605007100405	0.0
7	0.0	0.0	0.0	7.676011150265608	0.0
8	0.0	0.0	0.0	9.385999053279335	0.0
9	0.0	0.0	0.0	9.954241834534265	0.0
10	0.0	0.0	0.0	11.098301162362594	0.0
11	0.0	0.0	0.0	13.014463787934572	0.0
12	0.0	0.0	0.0	14.474727817808867	0.0
13	0.0	0.0	0.0	15.065691895019198	0.0
14	0.0	0.0	0.0	15.32572450428654	0.0
15	0.0	0.0	0.0	15.788565718192816	0.0
16	0.0	0.0	0.0	16.71782464629464	0.0
17	0.0	0.0	0.0	17.838110766317783	0.0
18	0.001051911849786988	0.0	0.0	19.044653658023456	0.0
19	0.0012622942197443854	0.0	0.0	19.761426392468312	0.0
20	0.0012622942197443854	0.0	0.0	20.402882238468415	0.0
21	0.0012622942197443854	0.0	0.0	21.17772050702151	0.0
22	0.0012622942197443854	0.0	0.0	21.86651238626203	0.0
23	0.0012622942197443854	0.0	0.0	22.500815231683585	0.0
24	0.0012622942197443854	0.0	0.0	23.055383158891285	0.0
25	0.0012622942197443854	0.0	0.0	23.533371903434492	0.0
26	0.0012622942197443854	0.0	0.0	24.01851364855625	0.0
27	0.0012622942197443854	0.0	0.0	24.48324830379214	0.0
28	0.0012622942197443854	0.0	0.0	24.939988428969652	0.0
29	0.0012622942197443854	0.0	0.0	25.474990795771316	0.0
30	0.0012622942197443854	0.0	0.0	26.066165255351603	0.0
31	0.0012622942197443854	0.0	0.0	26.568558354809866	0.0
32	0.0012622942197443854	0.0	0.0	27.12523010571714	0.0
33	0.0012622942197443854	0.0	0.0	27.61121338031873	0.0
34	0.0012622942197443854	0.0	0.0	28.11255456792721	0.0
35	0.001472676589701783	0.0	0.0	28.656603376637037	0.0
36	0.001472676589701783	0.0	0.0	29.189501919739126	0.0
37	0.001472676589701783	0.0	0.0	29.715878609372535	0.0
38	0.001472676589701783	0.0	0.0	30.20859411981276	0.0
39	0.001472676589701783	0.0	0.0	30.80439699153211	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGTA	45	3.8380676E-10	45.000004	31
GCGTAAG	45	3.8380676E-10	45.000004	1
TGATCGA	20	7.029108E-4	45.0	21
TGATCAT	20	7.029108E-4	45.0	30
AGTCCGC	20	7.029108E-4	45.0	23
AAATCGC	20	7.029108E-4	45.0	11
ATTAGGT	20	7.029108E-4	45.0	12
GGTCGCA	20	7.029108E-4	45.0	18
TTTACGG	25	3.887341E-5	45.0	2
TTTACGA	25	3.887341E-5	45.0	17
CGACGGA	20	7.029108E-4	45.0	3
ACGTTAT	20	7.029108E-4	45.0	44
ACGTTAA	20	7.029108E-4	45.0	43
GGGTCGT	55	1.8189894E-12	45.0	7
CGGCCAA	25	3.887341E-5	45.0	23
ACCGCGA	20	7.029108E-4	45.0	41
GACGTAA	25	3.887341E-5	45.0	20
TGGCGTT	35	1.209919E-7	45.0	35
ATTACGA	20	7.029108E-4	45.0	23
TCTTACG	20	7.029108E-4	45.0	28
>>END_MODULE