##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935150.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1244244
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.085545921860984	33.0	31.0	34.0	30.0	34.0
2	32.41497809111396	34.0	31.0	34.0	30.0	34.0
3	32.462729979007335	34.0	31.0	34.0	30.0	34.0
4	35.95802913254956	37.0	35.0	37.0	35.0	37.0
5	30.408261562844586	37.0	35.0	37.0	0.0	37.0
6	32.88986886816412	37.0	35.0	37.0	17.0	37.0
7	35.10383574282858	37.0	35.0	37.0	32.0	37.0
8	35.69219943998122	37.0	35.0	37.0	35.0	37.0
9	37.60612066443559	39.0	37.0	39.0	35.0	39.0
10	37.47946624617037	39.0	37.0	39.0	35.0	39.0
11	37.29732431902424	39.0	37.0	39.0	34.0	39.0
12	37.005889520061984	39.0	35.0	39.0	33.0	39.0
13	36.87081070915351	39.0	35.0	39.0	33.0	39.0
14	37.631645400741334	40.0	37.0	41.0	32.0	41.0
15	37.88881602000894	40.0	37.0	41.0	33.0	41.0
16	38.109073461475404	40.0	37.0	41.0	33.0	41.0
17	38.180016138313704	40.0	37.0	41.0	34.0	41.0
18	38.17329318043728	40.0	36.0	41.0	34.0	41.0
19	38.1815769254262	40.0	36.0	41.0	34.0	41.0
20	38.058967533699175	40.0	36.0	41.0	33.0	41.0
21	38.03553483078882	40.0	36.0	41.0	34.0	41.0
22	38.08060396513867	40.0	36.0	41.0	34.0	41.0
23	38.0479230761812	40.0	36.0	41.0	34.0	41.0
24	38.02800254612439	40.0	36.0	41.0	34.0	41.0
25	37.95581493662015	40.0	35.0	41.0	34.0	41.0
26	37.892153789771136	40.0	35.0	41.0	34.0	41.0
27	37.86027097578931	40.0	35.0	41.0	34.0	41.0
28	37.81923883096884	40.0	35.0	41.0	34.0	41.0
29	37.773398947473325	40.0	35.0	41.0	34.0	41.0
30	37.677261855391706	40.0	35.0	41.0	33.0	41.0
31	33.01653775304522	38.0	33.0	41.0	9.0	41.0
32	33.91157200677681	38.0	33.0	41.0	16.0	41.0
33	35.87492163916402	38.0	34.0	41.0	29.0	41.0
34	36.644064186767224	38.0	35.0	41.0	31.0	41.0
35	36.793605595044056	39.0	35.0	41.0	31.0	41.0
36	36.993499667267834	39.0	35.0	41.0	32.0	41.0
37	37.02639675176252	39.0	35.0	41.0	32.0	41.0
38	36.98989185400934	39.0	35.0	41.0	32.0	41.0
39	36.99793931093901	39.0	35.0	41.0	32.0	41.0
40	36.93786267002292	39.0	35.0	41.0	32.0	41.0
41	36.912051012502374	39.0	35.0	41.0	32.0	41.0
42	36.85913454274242	39.0	35.0	41.0	32.0	41.0
43	36.816752984141374	39.0	35.0	41.0	32.0	41.0
44	36.754618868967825	39.0	35.0	41.0	32.0	41.0
45	36.755396047720545	39.0	35.0	41.0	32.0	41.0
46	36.78098588379771	39.0	35.0	41.0	32.0	41.0
47	36.68140573713838	39.0	35.0	41.0	32.0	41.0
48	36.637582339155344	39.0	35.0	41.0	32.0	41.0
49	36.589209190480325	38.0	35.0	41.0	32.0	41.0
50	36.40002202140416	38.0	35.0	40.0	31.0	41.0
51	35.76728198006179	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	12.0
11	19.0
12	21.0
13	19.0
14	24.0
15	53.0
16	83.0
17	154.0
18	310.0
19	521.0
20	781.0
21	1235.0
22	2147.0
23	3323.0
24	4796.0
25	6229.0
26	7746.0
27	9375.0
28	11442.0
29	14685.0
30	20082.0
31	29346.0
32	43547.0
33	51894.0
34	102318.0
35	116454.0
36	135949.0
37	138255.0
38	208990.0
39	334111.0
40	318.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.52209052243772	21.49031861917759	23.944901482345905	11.042689376038783
2	33.556119217774004	23.166838658655376	25.093148932203007	18.18389319136761
3	34.300667714692615	23.36631721752325	25.233635846345248	17.09937922143888
4	29.48931238567355	25.444848438087707	25.305888555620925	19.759950620617822
5	22.584637739864526	38.691366002166774	21.465645002105695	17.258351255862998
6	27.633567049549768	32.19794509758536	24.75093309672379	15.417554756141078
7	80.5168439630812	6.026149211890916	8.22129743040754	5.235709394620348
8	81.57925615875986	4.669582493465912	8.287040162540467	5.464121185233765
9	76.13996933077435	6.612207894914503	10.521248243913574	6.726574530397575
10	44.95782177772206	21.810593420583103	18.25807478275965	14.973510018935194
11	33.433715573472725	25.35539652994107	23.162820154246273	18.048067742339928
12	30.66279604321982	22.910538447442786	28.218018330809713	18.208647178527684
13	28.67741375485837	23.61675041229855	30.134041233070043	17.571794599773035
14	20.732026837179845	26.539408669039194	33.194132340602	19.534432153178958
15	20.331381947592273	26.42809609690704	34.49106445359592	18.74945750190477
16	26.531773510661893	26.61977875722125	28.183378822803245	18.665068909313607
17	26.11786755652428	25.233796586521617	26.30673726375213	22.34159859320198
18	26.191647297475413	24.537711252776788	29.582300577700195	19.688340872047604
19	25.178903816293268	26.513850980997294	27.383455335127195	20.923789867582244
20	23.339634348246808	31.655125522003726	26.725626163357024	18.279613966392443
21	23.787134999244522	26.46707558967534	31.28799495918807	18.457794451892074
22	21.909448629047034	23.88767797956028	32.451753836064306	21.751119555328376
23	21.195119285284882	25.590720148138146	31.669672507964673	21.5444880586123
24	22.830972060142543	27.83224190753582	28.17389515239776	21.162890879923875
25	23.950768498783198	26.799566644484525	26.924863611960355	22.324801244771926
26	19.952838832254766	27.42806073406824	27.994750225839947	24.624350207837047
27	21.53878178235137	25.852807005699844	30.88887710127596	21.719534110672825
28	20.007731602483116	26.674028566744145	28.376106294263824	24.94213353650892
29	21.45423244958384	28.37401667197109	28.734878367908546	21.43687251053652
30	25.31633666708459	25.46518207039777	29.17104683647259	20.04743442604505
31	26.519396517081862	26.66599155792594	26.420702048794286	20.393909876197917
32	26.932418400249468	27.509877483837574	25.79494054220876	19.76276357370419
33	27.172483853649283	26.018690867707623	26.36524668794867	20.44357859069443
34	22.63454756462559	26.943589842506775	29.920417538682127	20.501445054185513
35	22.999347394883962	28.78896743725507	27.59201571395964	20.619669453901324
36	27.20503373936302	26.84875313845194	26.61857320589852	19.327639916286515
37	23.650184368982288	29.03763249009037	27.217892953472152	20.094290187455194
38	25.17086680747506	27.760632158965603	25.82242711236703	21.246073921192306
39	24.67860001735994	26.05582184844773	26.332536062058566	22.933042072133762
40	26.008885716949408	24.447696754012878	28.479140747313224	21.064276781724484
41	23.10382850952064	24.883543742224195	27.042525421058894	24.970102327196273
42	23.68691349928149	24.68253815168086	29.841333371910977	21.789214977126672
43	23.676304647641462	24.25199558928956	29.81304310087089	22.25865666219809
44	24.030897476700712	24.394893606077265	27.662821761648036	23.911387155573987
45	22.16116774523325	24.184806195569358	27.05377723340438	26.60024882579301
46	23.65830174788868	24.32336422759523	28.08484509469204	23.933488929824055
47	20.906269188358554	25.56693060203626	31.415060068603907	22.11174014100128
48	22.28871507517818	27.686450567573562	28.124949768694883	21.89988458855337
49	25.013662914990952	24.25191521920138	29.444626616644324	21.289795249163348
50	22.28493768103362	24.95402830955986	28.188442138358717	24.5725918710478
51	21.932755954619836	25.299619688742723	26.663500085192293	26.10412427144515
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1150.0
1	1597.0
2	2044.0
3	2352.5
4	2661.0
5	2286.5
6	1912.0
7	2005.0
8	2098.0
9	2254.5
10	2411.0
11	2474.0
12	2537.0
13	2692.0
14	2847.0
15	2866.0
16	2885.0
17	2966.5
18	3048.0
19	3181.0
20	3314.0
21	3950.5
22	4587.0
23	4926.5
24	5266.0
25	6188.5
26	9321.5
27	11532.0
28	12476.5
29	13421.0
30	15985.0
31	18549.0
32	21047.0
33	23545.0
34	25999.5
35	28454.0
36	31366.5
37	34279.0
38	39819.0
39	45359.0
40	49501.5
41	53644.0
42	58844.5
43	64045.0
44	73355.0
45	82665.0
46	93510.0
47	104355.0
48	118982.5
49	133610.0
50	132685.0
51	131760.0
52	115650.0
53	99540.0
54	90284.0
55	81028.0
56	75512.5
57	69997.0
58	67249.0
59	64501.0
60	53642.0
61	42783.0
62	36493.5
63	30204.0
64	27121.5
65	24039.0
66	19873.0
67	15707.0
68	12685.5
69	9664.0
70	8201.0
71	6738.0
72	5807.5
73	4877.0
74	3991.0
75	2466.0
76	1827.0
77	1205.5
78	584.0
79	519.0
80	454.0
81	272.5
82	91.0
83	52.0
84	13.0
85	7.0
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1244244.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.280041079926903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.13146212482802	17.47646202431063
2	13.269398020512016	6.70901854128889
3	5.203447179221713	3.94630075343864
4	2.5709664020641987	2.5997654503717924
5	1.4368895796638428	1.8162313800610423
6	0.9519919749755521	1.443983774108562
7	0.6733738186668973	1.1916042458632485
8	0.4915085421561376	0.9940284909473714
9	0.39789665178498207	0.9052959332420745
>10	4.019726053618727	24.336199782574962
>50	1.6789672849583381	29.254599836185225
>100	0.16605169440930537	6.314432792786658
>500	0.00542652596108782	0.9756440680354994
>1k	0.0028941471792468376	2.036432926785383
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	4778	0.38400828133388626	No Hit
CTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGCT	4310	0.34639508006468184	Illumina Single End Adapter 1 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC	3851	0.309505209589116	Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC	3228	0.2594346446516921	Illumina Single End Adapter 1 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2550	0.2049437248642549	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCC	1341	0.10777628825214347	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.36455871999382755	0.0
2	0.0	0.0	0.0	1.1565255689398541	0.0
3	0.0	0.0	0.0	1.6216272692494398	0.0
4	0.0	0.0	0.0	2.1703942313565507	0.0
5	0.0	0.0	0.0	3.5456871803279744	0.0
6	0.0	0.0	0.0	4.458691382076184	0.0
7	0.0	0.0	0.0	5.248809718994024	0.0
8	0.0	0.0	0.0	6.451869568991291	0.0
9	0.0	0.0	0.0	6.889886549583522	0.0
10	0.0	0.0	0.0	7.8146247842063135	0.0
11	0.0	0.0	0.0	9.40297883694838	0.0
12	0.0	0.0	0.0	10.5847406135774	0.0
13	8.037008818206075E-5	0.0	0.0	11.105538784997155	0.0
14	1.607401763641215E-4	0.0	0.0	11.339817592047861	0.0
15	1.607401763641215E-4	0.0	0.0	11.741989513310894	0.0
16	1.607401763641215E-4	0.0	0.0	12.475205827795834	0.0
17	1.607401763641215E-4	0.0	0.0	13.310894004712901	0.0
18	1.607401763641215E-4	0.0	0.0	14.211601582969257	0.0
19	1.607401763641215E-4	0.0	0.0	14.813010952835617	0.0
20	2.4111026454618227E-4	0.0	0.0	15.38717486280826	0.0
21	2.4111026454618227E-4	0.0	0.0	16.035279253908396	0.0
22	2.4111026454618227E-4	0.0	0.0	16.69953803273313	0.0
23	3.21480352728243E-4	0.0	0.0	17.306091088243143	0.0
24	3.21480352728243E-4	0.0	0.0	17.808002288940113	0.0
25	3.21480352728243E-4	0.0	0.0	18.285802463182463	0.0
26	3.21480352728243E-4	0.0	0.0	18.71779168716104	0.0
27	4.0185044091030375E-4	0.0	0.0	19.263986806446326	0.0
28	4.0185044091030375E-4	0.0	0.0	19.72723999472772	0.0
29	4.0185044091030375E-4	0.0	0.0	20.302127235494	0.0
30	4.0185044091030375E-4	0.0	0.0	21.008982161055226	0.0
31	4.0185044091030375E-4	0.0	0.0	21.482361980447564	0.0
32	4.0185044091030375E-4	0.0	0.0	22.004606813454597	0.0
33	4.0185044091030375E-4	0.0	0.0	22.531834591928913	0.0
34	4.0185044091030375E-4	0.0	0.0	23.129466567650717	0.0
35	4.0185044091030375E-4	0.0	0.0	23.750968459562593	0.0
36	4.0185044091030375E-4	0.0	0.0	24.279160679095096	0.0
37	4.0185044091030375E-4	0.0	0.0	24.877355245434174	0.0
38	4.0185044091030375E-4	0.0	0.0	25.556000270043498	0.0
39	4.0185044091030375E-4	0.0	0.0	26.896091120391176	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTCGT	30	2.165647E-6	45.000004	30
AGCCGTA	20	7.0335116E-4	45.0	12
CGAACCC	40	6.8157533E-9	45.0	19
GTCGATC	20	7.0335116E-4	45.0	21
CGCGCTA	20	7.0335116E-4	45.0	16
CGATTTA	25	3.8909922E-5	45.0	13
CCGTCAA	25	3.8909922E-5	45.0	27
CGCTATA	25	3.8909922E-5	45.0	18
CGTACAT	25	3.8909922E-5	45.0	35
CGTCGTG	20	7.0335116E-4	45.0	21
TATCGAT	20	7.0335116E-4	45.0	25
CGATATA	20	7.0335116E-4	45.0	19
ATCGAAT	50	2.1827873E-11	45.0	10
AGTCGAC	20	7.0335116E-4	45.0	12
TATCCCG	20	7.0335116E-4	45.0	1
CGCAATC	120	0.0	43.125004	20
CGTAAGG	230	0.0	43.04348	2
CGGTCTA	75	0.0	42.000004	31
TAAACGG	60	3.6379788E-12	41.250004	2
AATGCGA	55	6.184564E-11	40.90909	25
>>END_MODULE