SRR2935148.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935148.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	482464
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	3110	0.6446076805730583	Illumina PCR Primer Index 7 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	2430	0.5036645221197851	Illumina PCR Primer Index 7 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	2423	0.502213636665119	RNA PCR Primer, Index 14 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1026	0.21265835378390927	No Hit
TCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	850	0.1761789480665915	RNA PCR Primer, Index 14 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC	752	0.15586655170126681	No Hit
ACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	712	0.1475757776746037	RNA PCR Primer, Index 14 (95% over 24bp)
TGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCTT	625	0.1295433441666114	RNA PCR Primer, Index 14 (96% over 26bp)
GCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	555	0.11503448961995091	RNA PCR Primer, Index 14 (95% over 23bp)
AAAACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTC	548	0.11358360416528487	No Hit
AGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	517	0.10715825429462095	Illumina PCR Primer Index 7 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	511	0.10591463819062147	Illumina PCR Primer Index 7 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	491	0.10176925117728992	Illumina PCR Primer Index 7 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATCG	20	7.029211E-4	45.000004	43
GTCGCGC	20	7.029211E-4	45.000004	30
CCGGGTG	20	7.029211E-4	45.000004	3
TCGCAGG	20	7.029211E-4	45.000004	2
CAACGGC	20	7.029211E-4	45.000004	18
ACGTTGT	20	7.029211E-4	45.000004	25
CCAGGTA	20	7.029211E-4	45.000004	25
AGCCCGT	20	7.029211E-4	45.000004	35
ACCGCCC	20	7.029211E-4	45.000004	10
CGGTTTG	20	7.029211E-4	45.000004	32
GAACTCG	20	7.029211E-4	45.000004	16
CGTTGAG	20	7.029211E-4	45.000004	17
CGGCAGT	20	7.029211E-4	45.000004	43
CCGTGCC	20	7.029211E-4	45.000004	38
CCGTGAG	20	7.029211E-4	45.000004	4
TCGGGAC	20	7.029211E-4	45.000004	5
CGACCCG	20	7.029211E-4	45.000004	32
GCGCCTT	20	7.029211E-4	45.000004	9
CCTCGGC	20	7.029211E-4	45.000004	15
GCTTGCG	20	7.029211E-4	45.000004	1