##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935148.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	482464
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.922669877959805	33.0	31.0	34.0	30.0	34.0
2	32.252887262054784	34.0	31.0	34.0	30.0	34.0
3	32.306178284804666	34.0	31.0	34.0	30.0	34.0
4	35.86257005704053	37.0	35.0	37.0	35.0	37.0
5	30.356072162897128	37.0	35.0	37.0	0.0	37.0
6	32.830144839822246	37.0	35.0	37.0	17.0	37.0
7	35.05836912184122	37.0	35.0	37.0	32.0	37.0
8	35.630596272468	37.0	35.0	37.0	35.0	37.0
9	37.54935290508722	39.0	37.0	39.0	35.0	39.0
10	37.338835643695695	39.0	37.0	39.0	34.0	39.0
11	37.150796743383964	39.0	37.0	39.0	34.0	39.0
12	36.979368408834645	39.0	37.0	39.0	33.0	39.0
13	36.89550722955495	39.0	37.0	39.0	33.0	39.0
14	38.048782914372886	40.0	37.0	41.0	33.0	41.0
15	38.2184701863766	40.0	37.0	41.0	33.0	41.0
16	38.3087048981893	40.0	37.0	41.0	34.0	41.0
17	38.31074028321284	40.0	37.0	41.0	34.0	41.0
18	38.242500994892886	40.0	37.0	41.0	34.0	41.0
19	38.21832302513763	40.0	37.0	41.0	34.0	41.0
20	38.15612978377661	40.0	36.0	41.0	34.0	41.0
21	38.1129037606951	40.0	36.0	41.0	34.0	41.0
22	38.078667009351996	40.0	36.0	41.0	34.0	41.0
23	38.03236718180009	40.0	36.0	41.0	34.0	41.0
24	38.00788038071234	40.0	36.0	41.0	34.0	41.0
25	37.92175996551038	40.0	36.0	41.0	34.0	41.0
26	37.8742849207402	40.0	36.0	41.0	34.0	41.0
27	37.85751475757777	40.0	36.0	41.0	34.0	41.0
28	37.8268658386947	40.0	36.0	41.0	33.0	41.0
29	37.86117306161703	40.0	36.0	41.0	34.0	41.0
30	37.71995838031439	40.0	36.0	41.0	33.0	41.0
31	33.10405750480865	38.0	33.0	41.0	9.0	41.0
32	34.0154208396896	38.0	33.0	41.0	16.0	41.0
33	35.97576814021357	38.0	34.0	41.0	30.0	41.0
34	36.827771605757114	38.0	35.0	41.0	31.0	41.0
35	37.00134932347284	39.0	35.0	41.0	32.0	41.0
36	37.139115042780396	39.0	35.0	41.0	33.0	41.0
37	37.22628216820323	39.0	35.0	41.0	33.0	41.0
38	37.23459574185846	39.0	35.0	41.0	33.0	41.0
39	37.22858907607614	39.0	35.0	41.0	33.0	41.0
40	37.213246998739805	39.0	35.0	41.0	33.0	41.0
41	37.12864794057173	39.0	35.0	41.0	33.0	41.0
42	37.04220833056974	39.0	35.0	41.0	32.0	41.0
43	37.01182678914903	39.0	35.0	41.0	32.0	41.0
44	36.93221048617099	39.0	35.0	41.0	32.0	41.0
45	36.91841463819062	39.0	35.0	41.0	32.0	41.0
46	36.85397666976189	39.0	35.0	41.0	32.0	41.0
47	36.73767576440937	39.0	35.0	41.0	31.0	41.0
48	36.77082642435498	39.0	35.0	41.0	32.0	41.0
49	36.767912217284604	39.0	35.0	41.0	32.0	41.0
50	36.58442495191351	39.0	35.0	41.0	31.0	41.0
51	35.98354488625058	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	6.0
12	4.0
13	3.0
14	9.0
15	18.0
16	28.0
17	60.0
18	136.0
19	222.0
20	417.0
21	677.0
22	1100.0
23	1592.0
24	2161.0
25	2633.0
26	2984.0
27	3454.0
28	4271.0
29	5225.0
30	7089.0
31	10117.0
32	16120.0
33	17579.0
34	32136.0
35	49696.0
36	53461.0
37	53666.0
38	83428.0
39	134036.0
40	132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.106279432247796	22.53038568680772	25.328729190157194	11.034605690787291
2	30.656795118392253	25.177837102871926	25.7051303309677	18.460237447768122
3	29.888654904821916	26.44819095310738	26.791014459109903	16.872139682960803
4	27.388157458380313	27.031861444584464	26.69235424819261	18.887626848842608
5	20.594489951581878	40.582302513762684	21.585651986469458	17.23755554818598
6	25.03191948000265	34.20731909531074	24.16843536512569	16.59232605956092
7	80.61326855475227	5.963139218677456	8.251807388737813	5.17178483783246
8	80.43398056642567	4.839946607415269	8.543020826424355	6.183051999734696
9	75.01969058831332	6.647749883929163	10.870033826358029	7.462525701399484
10	39.66409929030974	22.78055979306228	20.392609604032632	17.162731312595344
11	27.24244710486171	29.33503846919148	25.5494710486171	17.873043377329708
12	25.65538568680772	25.81581216422365	28.462434502885188	20.06636764608344
13	21.936973535849308	29.14932513099423	30.659904158652253	18.25379717450421
14	16.59419148371692	32.87830801883664	28.861842541619687	21.665657955826756
15	15.181029050872189	31.024076407773432	34.726321549379854	19.06857299197453
16	16.467549910459642	31.868077203687733	31.27528686078132	20.3890860250713
17	17.49249684950587	30.635653644624263	27.371368640976325	24.500480864893547
18	17.767128739139086	29.813001591828613	31.714283345493133	20.705586323539166
19	17.31631790143928	31.152168866485376	29.440953107382107	22.090560124693244
20	20.60485341911521	31.605052397691846	28.85748988525569	18.932604297937257
21	19.38051336472773	30.844995688797503	30.82468329243218	18.94980765404258
22	18.477855342574784	28.044786761292034	28.232987331697284	25.244370564435897
23	17.284398421436624	29.04506864760894	29.101031372288915	24.569501558665515
24	19.798161106320887	27.110416528487097	30.68954367579757	22.40187868939444
25	17.48772965444054	31.69044737016648	27.70009783113351	23.121725144259468
26	18.997479604695894	30.924379850102806	27.910890760761426	22.167249784439875
27	19.18485109769848	30.253863500696426	29.066002852026262	21.49528254957883
28	16.347333687073025	28.73209192810241	30.80996716853485	24.11060721628971
29	18.08694534721762	26.30828414140744	29.327991311268818	26.27677920010612
30	19.344655767062413	28.778520262651718	26.6359769848113	25.240846985474562
31	21.618400543874777	30.141730781985803	25.879236585527625	22.360632088611794
32	18.788759368574652	29.843470186376603	26.324451150759437	25.04331929428931
33	18.269964183856207	29.458778271539433	25.51610068315978	26.755156861444583
34	17.149880612854016	28.61560655302779	28.972109836174305	25.262402997943887
35	18.076789149034955	26.81008323937123	28.272575777674604	26.840551833919214
36	19.44476686343437	27.784663726205476	28.508448298733168	24.262121111626982
37	16.677306493334218	28.705768720567754	32.13234562578762	22.484579160310407
38	16.779904821914172	28.567105524971808	28.821010479538366	25.831979173575647
39	19.64457451747695	27.625273595542883	28.482954168601182	24.247197718378988
40	20.339134111560657	26.52964780791935	29.671436625323338	23.45978145519666
41	17.281703919877963	27.558325595277577	29.175233799827556	25.984736685016912
42	20.632627512104527	26.88967467002719	29.372554221662135	23.105143596206144
43	21.117637792664322	25.55921270809843	29.483443324268755	23.839706174968494
44	18.546254228294753	25.526878689394444	28.241070836373282	27.68579624593752
45	18.877677920010612	24.954193473502688	28.003747429860056	28.16438117662665
46	21.810124693241363	24.911081448564037	29.583347151290045	23.69544670690456
47	17.044380513364725	26.320720302447437	32.01440936525834	24.620489818929496
48	17.40046925780991	23.584764873648602	32.76534622272335	26.249419645818133
49	20.01227034555946	21.831887975061353	32.57009849439544	25.58574318498375
50	18.465419181534788	23.01684685282218	32.04384161305299	26.47389235259004
51	17.407930954433905	23.253133912582076	29.362190754128804	29.97674437885521
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1251.0
1	1234.0
2	1217.0
3	1289.0
4	1361.0
5	1130.0
6	899.0
7	890.0
8	881.0
9	984.0
10	1087.0
11	1204.5
12	1322.0
13	1334.0
14	1346.0
15	1420.0
16	1494.0
17	1558.0
18	1622.0
19	1620.5
20	1619.0
21	1886.0
22	2153.0
23	2180.0
24	2207.0
25	2140.5
26	3615.5
27	5157.0
28	6136.5
29	7116.0
30	8552.0
31	9988.0
32	10732.5
33	11477.0
34	13049.5
35	14622.0
36	15372.0
37	16122.0
38	18289.0
39	20456.0
40	22551.5
41	24647.0
42	28701.0
43	32755.0
44	36648.0
45	40541.0
46	46275.0
47	52009.0
48	56822.0
49	61635.0
50	58169.0
51	54703.0
52	46161.0
53	37619.0
54	30880.0
55	24141.0
56	20272.5
57	16404.0
58	13798.0
59	11192.0
60	9347.5
61	7503.0
62	6776.5
63	6050.0
64	4653.5
65	3257.0
66	2484.0
67	1711.0
68	1523.5
69	1336.0
70	981.5
71	627.0
72	574.5
73	522.0
74	392.0
75	143.5
76	25.0
77	28.0
78	31.0
79	27.0
80	23.0
81	11.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	482464.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.998580779661317
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.48513767111069	21.14454096192363
2	12.59180161849985	7.554723560280757
3	5.317886346758708	4.785871294508973
4	2.827146049989739	3.3924147662607034
5	1.6645585952085935	2.4967197740422273
6	1.048943165595792	1.888008377183941
7	0.6661769899422159	1.3989054972433232
8	0.49302548565814075	1.1832051886357988
9	0.35443561212010194	0.9569308807236224
>10	3.28628587074216	24.635859774259714
>50	1.1758048335099742	23.253425914743133
>100	0.07961178560223782	4.080906093470341
>500	0.006123983507864449	1.164267283433382
>1k	0.0030619917539322244	2.064220633290468
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	3110	0.6446076805730583	Illumina PCR Primer Index 7 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	2430	0.5036645221197851	Illumina PCR Primer Index 7 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	2423	0.502213636665119	RNA PCR Primer, Index 14 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1026	0.21265835378390927	No Hit
TCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	850	0.1761789480665915	RNA PCR Primer, Index 14 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC	752	0.15586655170126681	No Hit
ACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	712	0.1475757776746037	RNA PCR Primer, Index 14 (95% over 24bp)
TGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCTT	625	0.1295433441666114	RNA PCR Primer, Index 14 (96% over 26bp)
GCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	555	0.11503448961995091	RNA PCR Primer, Index 14 (95% over 23bp)
AAAACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTC	548	0.11358360416528487	No Hit
AGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	517	0.10715825429462095	Illumina PCR Primer Index 7 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	511	0.10591463819062147	Illumina PCR Primer Index 7 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	491	0.10176925117728992	Illumina PCR Primer Index 7 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6746617364197122	0.0
2	0.0	0.0	0.0	2.07828977913378	0.0
3	0.0	0.0	0.0	2.9025999867347614	0.0
4	0.0	0.0	0.0	3.7700222192743915	0.0
5	0.0	0.0	0.0	6.0974497579093985	0.0
6	0.0	0.0	0.0	7.630621144790077	0.0
7	0.0	0.0	0.0	8.847292233202891	0.0
8	0.0	0.0	0.0	10.895320687139352	0.0
9	0.0	0.0	0.0	11.589880281223055	0.0
10	0.0	0.0	0.0	13.124917092259734	0.0
11	0.0	0.0	0.0	15.491518538170723	0.0
12	0.0	0.0	0.0	17.164389467400678	0.0
13	0.0	0.0	0.0	17.84630563109372	0.0
14	0.0	0.0	0.0	18.1279846786496	0.0
15	0.0	0.0	0.0	18.64823074882271	0.0
16	0.0	0.0	0.0	19.794637527359555	0.0
17	0.0	0.0	0.0	21.04322809577502	0.0
18	0.0	0.0	0.0	22.461572262386415	0.0
19	0.0	0.0	0.0	23.365473900643366	0.0
20	0.0	0.0	0.0	24.113094448497712	0.0
21	2.0726935066657824E-4	0.0	0.0	25.047671950653314	0.0
22	2.0726935066657824E-4	0.0	0.0	25.91820322345294	0.0
23	2.0726935066657824E-4	0.0	0.0	26.77920010612191	0.0
24	2.0726935066657824E-4	0.0	0.0	27.45987265371095	0.0
25	2.0726935066657824E-4	0.0	0.0	28.105309411686676	0.0
26	2.0726935066657824E-4	0.0	0.0	28.632809909133115	0.0
27	2.0726935066657824E-4	0.0	0.0	29.148496053591565	0.0
28	2.0726935066657824E-4	0.0	0.0	29.641382569476686	0.0
29	2.0726935066657824E-4	0.0	0.0	30.22215129004444	0.0
30	2.0726935066657824E-4	0.0	0.0	30.88064601711216	0.0
31	2.0726935066657824E-4	0.0	0.0	31.436956954301255	0.0
32	2.0726935066657824E-4	0.0	0.0	32.07099389799031	0.0
33	2.0726935066657824E-4	0.0	0.0	32.64803177024607	0.0
34	4.145387013331565E-4	0.0	0.0	33.22341148769649	0.0
35	4.145387013331565E-4	0.0	0.0	33.8392087285269	0.0
36	4.145387013331565E-4	0.0	0.0	34.415832062081314	0.0
37	4.145387013331565E-4	0.0	0.0	35.04717450421171	0.0
38	4.145387013331565E-4	0.0	0.0	35.62545599257147	0.0
39	4.145387013331565E-4	0.0	0.0	36.200421171320556	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATCG	20	7.029211E-4	45.000004	43
GTCGCGC	20	7.029211E-4	45.000004	30
CCGGGTG	20	7.029211E-4	45.000004	3
TCGCAGG	20	7.029211E-4	45.000004	2
CAACGGC	20	7.029211E-4	45.000004	18
ACGTTGT	20	7.029211E-4	45.000004	25
CCAGGTA	20	7.029211E-4	45.000004	25
AGCCCGT	20	7.029211E-4	45.000004	35
ACCGCCC	20	7.029211E-4	45.000004	10
CGGTTTG	20	7.029211E-4	45.000004	32
GAACTCG	20	7.029211E-4	45.000004	16
CGTTGAG	20	7.029211E-4	45.000004	17
CGGCAGT	20	7.029211E-4	45.000004	43
CCGTGCC	20	7.029211E-4	45.000004	38
CCGTGAG	20	7.029211E-4	45.000004	4
TCGGGAC	20	7.029211E-4	45.000004	5
CGACCCG	20	7.029211E-4	45.000004	32
GCGCCTT	20	7.029211E-4	45.000004	9
CCTCGGC	20	7.029211E-4	45.000004	15
GCTTGCG	20	7.029211E-4	45.000004	1
>>END_MODULE