SRR2935147.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935147.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1271325
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGCT	6093	0.47926376025013273	Illumina PCR Primer Index 11 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	5793	0.4556663323697717	RNA PCR Primer, Index 14 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	4725	0.37165948911568636	Illumina PCR Primer Index 11 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGC	2419	0.19027392680864452	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	2257	0.17753131575324957	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCG	2124	0.16706978939295616	No Hit
TCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	1970	0.1549564430810375	RNA PCR Primer, Index 14 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCC	1631	0.12829134957622954	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTC	1609	0.12656087153166973	No Hit
ACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	1552	0.1220773602344011	RNA PCR Primer, Index 14 (95% over 23bp)
TGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGCTT	1455	0.11444752521975106	RNA PCR Primer, Index 14 (96% over 25bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	55	1.8189894E-12	45.000004	1
TAGCGAG	60	0.0	45.000004	1
AATTGCG	55	1.8189894E-12	45.000004	1
ACTCGAG	30	2.1656851E-6	45.000004	1
CGTAACA	30	2.1656851E-6	45.000004	15
GCGTAAA	30	2.1656851E-6	45.000004	41
TCTTGCG	35	1.2120654E-7	45.0	1
CGAAAAC	20	7.033569E-4	45.0	6
GACCGGT	20	7.033569E-4	45.0	19
CGTTCGA	45	3.8562575E-10	45.0	19
AATGCGT	20	7.033569E-4	45.0	28
CTAACGG	90	0.0	45.0	2
TACTACC	20	7.033569E-4	45.0	6
TTAACGG	80	0.0	42.1875	2
TATCTCG	235	0.0	42.12766	14
TTACGGG	310	0.0	42.09677	3
ATCTCGA	225	0.0	42.0	15
TATTGCG	60	3.6379788E-12	41.250004	1
CGTAAGG	175	0.0	41.142857	2
CGCATCG	55	6.184564E-11	40.909092	21