##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935145.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	758831
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01875779982631	33.0	31.0	34.0	30.0	34.0
2	32.348072232157094	34.0	31.0	34.0	30.0	34.0
3	32.39767220896353	34.0	31.0	34.0	30.0	34.0
4	35.90606735887174	37.0	35.0	37.0	35.0	37.0
5	30.373815777162505	37.0	35.0	37.0	0.0	37.0
6	32.859640684157604	37.0	35.0	37.0	17.0	37.0
7	35.07153503217449	37.0	35.0	37.0	32.0	37.0
8	35.63761100956603	37.0	35.0	37.0	35.0	37.0
9	37.501927306607136	39.0	37.0	39.0	35.0	39.0
10	37.465950916607255	39.0	37.0	39.0	35.0	39.0
11	37.32305348621762	39.0	37.0	39.0	35.0	39.0
12	36.98658726383081	39.0	35.0	39.0	33.0	39.0
13	36.805407264595146	39.0	35.0	39.0	33.0	39.0
14	37.642850647904474	40.0	36.0	41.0	33.0	41.0
15	37.89506622686738	40.0	37.0	41.0	33.0	41.0
16	38.10356324398977	40.0	36.0	41.0	33.0	41.0
17	38.16519488529067	40.0	36.0	41.0	34.0	41.0
18	38.140003505391846	40.0	36.0	41.0	34.0	41.0
19	38.1227190243941	40.0	36.0	41.0	34.0	41.0
20	38.02559331392629	40.0	36.0	41.0	33.0	41.0
21	37.983815895765986	40.0	36.0	41.0	34.0	41.0
22	38.01169166784172	40.0	36.0	41.0	34.0	41.0
23	37.96721140807374	40.0	35.0	41.0	34.0	41.0
24	37.938215491987016	40.0	35.0	41.0	34.0	41.0
25	37.893113222838814	40.0	35.0	41.0	34.0	41.0
26	37.82838734843463	40.0	35.0	41.0	34.0	41.0
27	37.768957778477684	40.0	35.0	41.0	34.0	41.0
28	37.72405449961849	40.0	35.0	41.0	34.0	41.0
29	37.69431138158562	40.0	35.0	41.0	33.0	41.0
30	37.543583485650956	39.0	35.0	41.0	33.0	41.0
31	32.90433179456295	38.0	33.0	41.0	9.0	41.0
32	33.70554840274053	38.0	33.0	40.0	16.0	41.0
33	35.66302378263408	38.0	34.0	40.0	28.0	41.0
34	36.495464734571996	38.0	35.0	40.0	31.0	41.0
35	36.51774505785873	38.0	35.0	40.0	31.0	41.0
36	36.7286339118987	39.0	35.0	40.0	31.0	41.0
37	36.8144105868105	39.0	35.0	41.0	31.0	41.0
38	36.75791447634585	39.0	35.0	41.0	31.0	41.0
39	36.81341036409952	39.0	35.0	41.0	32.0	41.0
40	36.76571199647879	39.0	35.0	41.0	32.0	41.0
41	36.62987015554188	39.0	35.0	41.0	31.0	41.0
42	36.56307794489155	39.0	35.0	41.0	31.0	41.0
43	36.604927842958446	39.0	35.0	41.0	31.0	41.0
44	36.60819207438811	39.0	35.0	40.0	31.0	41.0
45	36.62077458617268	39.0	35.0	40.0	32.0	41.0
46	36.6087442394947	38.0	35.0	40.0	32.0	41.0
47	36.490370056046736	38.0	35.0	40.0	31.0	41.0
48	36.46053337304354	38.0	35.0	40.0	31.0	41.0
49	36.45544396578421	38.0	35.0	40.0	31.0	41.0
50	36.269652926672734	38.0	35.0	40.0	31.0	41.0
51	35.58374789643544	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	6.0
11	11.0
12	10.0
13	17.0
14	21.0
15	36.0
16	56.0
17	91.0
18	177.0
19	337.0
20	545.0
21	869.0
22	1414.0
23	2193.0
24	2978.0
25	3769.0
26	4630.0
27	5668.0
28	6971.0
29	8975.0
30	12354.0
31	18364.0
32	27489.0
33	33236.0
34	63568.0
35	77227.0
36	84984.0
37	89141.0
38	134302.0
39	179228.0
40	158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.083201661503026	20.62066520740455	24.140816598162175	11.155316532930257
2	33.32454789010992	23.838904841789542	24.297768541348468	18.53877872675207
3	32.474160913299535	22.970595560803396	26.459910045846836	18.095333480050236
4	29.115573823420497	25.42357916321289	26.445545846176554	19.01530116719006
5	22.76686113245242	38.38957554448882	21.486602418720373	17.356960904338383
6	27.189057906174103	32.960962322308916	23.68445674992192	16.165523021595057
7	77.53557775051362	6.378231780198754	9.972708020626463	6.1134824486611645
8	77.5828873622717	5.600983618223294	9.835259761396149	6.9808692581088545
9	71.81467283229071	7.6773352696450194	11.951673033916643	8.556318864147617
10	43.36367386150539	23.644922255416557	18.147782576094016	14.843621306984032
11	33.113565471099626	23.879625371130068	23.18184154311039	19.824967614659915
12	29.085000481003014	21.755832326301906	29.794117530780902	19.36504966191418
13	26.030565435518582	23.849052028712585	31.326342756160464	18.794039779608372
14	19.73390649564923	28.015197059687864	31.67530056099448	20.57559588366843
15	18.950991722794665	25.606623872772726	35.41065138350963	20.031733020922974
16	23.5953723556365	26.21097451211139	29.534244120232305	20.659409012019804
17	23.702379054097683	26.19489715101254	25.606755654421075	24.495968140468694
18	24.19906408673341	25.12509372969739	29.493260027595074	21.18258215597412
19	23.617643454207855	26.879107469252045	27.293824316613318	22.209424759926783
20	23.493373359812658	29.826799379572	27.285126727822135	19.394700532793205
21	23.351049179593346	26.7873874419996	30.47463796286657	19.38692541554048
22	20.273025219054045	24.11327423365677	30.736224534843725	24.87747601244546
23	20.368303350811974	26.722550871010803	30.629217836382537	22.279927941794682
24	23.149950384209394	26.193579334529034	28.101514039357905	22.554956241903664
25	21.770196525972185	26.83865050320822	27.099446385295277	24.291706585524313
26	19.336980170815373	27.382249802656982	28.007158379138435	25.27361164738921
27	21.651988387401147	27.281700404965004	28.982474358585776	22.083836849048076
28	19.922617816088167	27.042648494855904	28.089258346061246	24.945475342994687
29	21.01073888652414	27.844144480127987	28.92343618012443	22.221680453223446
30	25.40921496354261	27.553697727161914	26.59524979870353	20.441837510591952
31	27.38238158430533	26.83693734177966	25.260960609147492	20.51972046476752
32	25.991953412551677	27.765339054413964	24.851515027720268	21.391192505314095
33	27.95536819133641	25.131814593763302	25.21773622848829	21.695080986411995
34	24.400294663765713	24.230428119040997	29.573383269792615	21.795893947400675
35	22.72047399223279	27.44932666166775	28.66079535496046	21.169403991139003
36	28.02679384474277	25.795730538156718	26.35856995826475	19.81890565883576
37	24.42256576233707	28.963761364519904	27.19538342529496	19.41828944784807
38	24.78430638706115	27.361691865514192	25.616639278047415	22.23736246937724
39	26.157998289474204	24.37262051761196	26.334585698264828	23.13479549464901
40	27.266413733756266	22.71941973904598	28.852537653311476	21.16162887388628
41	23.147446532890722	23.562031598603642	27.50717880529393	25.783343063211706
42	23.30677054574734	23.42840500717551	30.19512908671364	23.069695360363507
43	24.82660829618189	23.556760332669594	28.695585710125183	22.92104566102334
44	23.361723493109796	24.661090545852765	26.8141391166149	25.16304684442254
45	22.2388120675091	23.675363816185683	26.889913564416844	27.195910551888364
46	24.92649878563211	23.477559562010512	27.633425624414393	23.962516027942982
47	21.333472143336262	24.62550950079794	31.49304654132475	22.54797181454105
48	22.31537720520116	24.39146529332618	29.74680791902281	23.546349582449846
49	24.961552704093535	22.222866488058607	30.397282135284403	22.418298672563456
50	22.23986632069591	23.28278628574742	29.380323154958088	25.097024238598582
51	21.765584168279894	23.937872859701304	26.596962960132096	27.699580011886702
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1045.0
1	1194.0
2	1343.0
3	1527.5
4	1712.0
5	1379.0
6	1046.0
7	1059.5
8	1073.0
9	1250.5
10	1428.0
11	1553.5
12	1679.0
13	1759.5
14	1840.0
15	1898.0
16	1956.0
17	1959.0
18	1962.0
19	2209.5
20	2457.0
21	2375.0
22	2293.0
23	2761.5
24	3230.0
25	3346.0
26	3885.5
27	4309.0
28	5784.0
29	7259.0
30	8441.0
31	9623.0
32	10804.5
33	11986.0
34	14441.5
35	16897.0
36	18125.5
37	19354.0
38	20832.0
39	22310.0
40	25988.5
41	29667.0
42	34538.5
43	39410.0
44	45298.5
45	51187.0
46	59075.5
47	66964.0
48	77481.5
49	87999.0
50	87379.0
51	86759.0
52	75390.0
53	64021.0
54	56521.0
55	49021.0
56	44385.0
57	39749.0
58	37312.0
59	34875.0
60	31293.5
61	27712.0
62	23383.0
63	19054.0
64	17113.0
65	15172.0
66	13179.5
67	11187.0
68	9484.0
69	7781.0
70	5996.5
71	4212.0
72	3377.0
73	2542.0
74	1884.5
75	1102.5
76	978.0
77	798.5
78	619.0
79	457.5
80	296.0
81	177.5
82	59.0
83	46.5
84	34.0
85	38.0
86	42.0
87	21.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	758831.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.937042417170026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.4867410208055	19.133169889119817
2	13.344395984909374	7.456059133238532
3	5.623476916754386	4.713099394660314
4	2.9057008152717243	3.2470674771140677
5	1.6635951374010576	2.3237963959285577
6	0.9976697417450017	1.6723165136074307
7	0.7161101908268493	1.4004200543548186
8	0.5162586957151896	1.1538192864342498
9	0.3757652125536201	0.9447991813806655
>10	4.015188735200335	26.977191421761958
>50	1.2557344705247921	23.257328691134877
>100	0.08915822160268964	4.318866641613868
>500	0.007519368086973826	1.5375543368174722
>1k	0.002685488602490652	1.8645115828334058
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGCT	3495	0.4605768609874926	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	3184	0.41959276835026504	TruSeq Adapter, Index 21 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	2962	0.3903372424162956	TruSeq Adapter, Index 21 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1767	0.23285817263659497	No Hit
TCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	1018	0.13415371802153575	TruSeq Adapter, Index 21 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	924	0.12176624307652165	No Hit
GCCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTG	871	0.11478181571390732	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCC	852	0.11227796439523426	No Hit
ACTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	848	0.11175083780182939	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGC	826	0.1088516415381027	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCG	789	0.10397572054910778	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	786	0.10358037560405413	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.48482468428411596	0.0
2	0.0	0.0	0.0	1.59798426790682	0.0
3	0.0	0.0	0.0	2.287861197025425	0.0
4	0.0	0.0	0.0	3.032295728561432	0.0
5	0.0	0.0	0.0	4.965268946577037	0.0
6	0.0	0.0	0.0	6.072234792727234	0.0
7	0.0	0.0	0.0	7.075883826570079	0.0
8	0.0	0.0	0.0	8.639604865905584	0.0
9	0.0	0.0	0.0	9.26108711952991	0.0
10	0.0	0.0	0.0	10.574423026998106	0.0
11	0.0	0.0	0.0	12.507396245013712	0.0
12	0.0	0.0	0.0	14.071249065997568	0.0
13	0.0	0.0	0.0	14.737800643358007	0.0
14	0.0	0.0	0.0	15.045510792258092	0.0
15	0.0	0.0	0.0	15.59569917412441	0.0
16	0.0	0.0	0.0	16.59262734390134	0.0
17	0.0	0.0	0.0	17.66164007532639	0.0
18	0.0	0.0	0.0	18.877721126311393	0.0
19	0.0	0.0	0.0	19.718356261143786	0.0
20	0.0	0.0	0.0	20.492573445207167	0.0
21	0.0	0.0	0.0	21.28326333531445	0.0
22	0.0	0.0	0.0	22.067364143004173	0.0
23	0.0	0.0	0.0	22.811139766298425	0.0
24	0.0	0.0	0.0	23.437629722560096	0.0
25	0.0	0.0	0.0	24.024716965964753	0.0
26	0.0	0.0	0.0	24.562122527941003	0.0
27	0.0	0.0	0.0	25.200868177499338	0.0
28	0.0	0.0	0.0	25.754351100574436	0.0
29	0.0	0.0	0.0	26.455561251451247	0.0
30	0.0	0.0	0.0	27.212119694635565	0.0
31	0.0	0.0	0.0	27.774168424853492	0.0
32	0.0	0.0	0.0	28.38853446946685	0.0
33	0.0	0.0	0.0	28.989195222651684	0.0
34	0.0	0.0	0.0	29.623460296166076	0.0
35	0.0	0.0	0.0	30.279073996713365	0.0
36	0.0	0.0	0.0	30.85192882209609	0.0
37	0.0	0.0	0.0	31.52243384890707	0.0
38	0.0	0.0	0.0	32.264496310772756	0.0
39	0.0	0.0	0.0	33.45988764296661	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCTA	45	3.8380676E-10	45.000004	31
ACGCTAA	65	0.0	45.000004	25
CTTCGTC	20	7.031769E-4	45.0	29
CTTAACG	25	3.8895487E-5	45.0	1
AACGTAG	25	3.8895487E-5	45.0	1
CGGGTAG	55	1.8189894E-12	45.0	6
TCGCAAG	20	7.031769E-4	45.0	2
AAATCCG	25	3.8895487E-5	45.0	15
CTCGTAT	20	7.031769E-4	45.0	28
CTATCGA	20	7.031769E-4	45.0	11
TTAGACG	35	1.2111923E-7	45.0	1
GGTCCGT	20	7.031769E-4	45.0	9
GATTTCG	20	7.031769E-4	45.0	9
TTCGTCG	20	7.031769E-4	45.0	30
TTCGTAG	35	1.2111923E-7	45.0	1
TAAGTCG	20	7.031769E-4	45.0	25
TAACGCG	20	7.031769E-4	45.0	1
CAGATAG	40	6.8102963E-9	45.0	1
CGCTATC	20	7.031769E-4	45.0	1
TACGACG	35	1.2111923E-7	45.0	1
>>END_MODULE