##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935142.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	683252
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.977554401597068	33.0	31.0	34.0	30.0	34.0
2	32.308366459227344	34.0	31.0	34.0	30.0	34.0
3	32.37222137659312	34.0	31.0	34.0	30.0	34.0
4	35.88195716953628	37.0	35.0	37.0	35.0	37.0
5	30.416118796578715	37.0	35.0	37.0	0.0	37.0
6	32.85816214222571	37.0	35.0	37.0	17.0	37.0
7	35.03049240982829	37.0	35.0	37.0	32.0	37.0
8	35.594099980680625	37.0	35.0	37.0	35.0	37.0
9	37.4397440475842	39.0	37.0	39.0	35.0	39.0
10	37.3696000889862	39.0	37.0	39.0	34.0	39.0
11	37.27503175987776	39.0	37.0	39.0	34.0	39.0
12	37.118039903286046	39.0	37.0	39.0	34.0	39.0
13	37.06423837764105	39.0	37.0	39.0	33.0	39.0
14	38.26438122391153	40.0	38.0	41.0	33.0	41.0
15	38.38252943277151	40.0	38.0	41.0	34.0	41.0
16	38.45758958627271	40.0	38.0	41.0	34.0	41.0
17	38.43873124410906	40.0	38.0	41.0	34.0	41.0
18	38.385087786058435	40.0	37.0	41.0	34.0	41.0
19	38.36067658784753	40.0	37.0	41.0	34.0	41.0
20	38.2686739299702	40.0	37.0	41.0	34.0	41.0
21	38.23606370709489	40.0	37.0	41.0	34.0	41.0
22	38.21302242803534	40.0	37.0	41.0	34.0	41.0
23	38.15079648504505	40.0	37.0	41.0	34.0	41.0
24	38.114805371956464	40.0	37.0	41.0	34.0	41.0
25	38.07028739030402	40.0	36.0	41.0	34.0	41.0
26	37.98682184611241	40.0	36.0	41.0	34.0	41.0
27	37.94536130154028	40.0	36.0	41.0	34.0	41.0
28	37.855985785625215	40.0	36.0	41.0	33.0	41.0
29	37.87912805231452	40.0	36.0	41.0	34.0	41.0
30	37.74131213666407	40.0	36.0	41.0	33.0	41.0
31	33.10033194194821	38.0	33.0	41.0	9.0	41.0
32	34.00374386024483	38.0	33.0	41.0	16.0	41.0
33	35.993545573229206	39.0	34.0	41.0	30.0	41.0
34	36.79929074484963	39.0	35.0	41.0	31.0	41.0
35	36.964992418609825	39.0	35.0	41.0	32.0	41.0
36	37.118325303109245	39.0	35.0	41.0	32.0	41.0
37	37.18781650108598	39.0	35.0	41.0	32.0	41.0
38	37.16705695702318	39.0	35.0	41.0	32.0	41.0
39	37.19902905516559	39.0	35.0	41.0	32.0	41.0
40	37.1722439158612	39.0	35.0	41.0	32.0	41.0
41	37.13135856170198	39.0	35.0	41.0	32.0	41.0
42	37.054758712744345	39.0	35.0	41.0	32.0	41.0
43	37.003537494218826	39.0	35.0	41.0	32.0	41.0
44	36.901471199498864	39.0	35.0	41.0	32.0	41.0
45	36.85986868680955	39.0	35.0	41.0	32.0	41.0
46	36.80140416713014	39.0	35.0	41.0	31.0	41.0
47	36.71114317996874	39.0	35.0	41.0	31.0	41.0
48	36.71527781843302	39.0	35.0	41.0	31.0	41.0
49	36.670530053333174	39.0	35.0	41.0	31.0	41.0
50	36.49392025197145	39.0	35.0	41.0	31.0	41.0
51	35.84845415747045	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	4.0
11	6.0
12	12.0
13	10.0
14	14.0
15	31.0
16	47.0
17	79.0
18	171.0
19	299.0
20	491.0
21	855.0
22	1332.0
23	2122.0
24	2993.0
25	3584.0
26	4371.0
27	5174.0
28	6293.0
29	7709.0
30	9922.0
31	13986.0
32	21667.0
33	25142.0
34	44381.0
35	66892.0
36	77719.0
37	77264.0
38	119255.0
39	191273.0
40	149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.70693682565144	21.36093271589399	23.22042233319478	12.711708125259788
2	33.095695292512865	24.177463073653644	24.73348047279774	17.993361161035754
3	31.3584153430945	24.50311158986728	25.73647790273574	18.40199516430248
4	27.581917067202145	25.8304403060657	26.39875185143988	20.18889077529228
5	22.228840896184717	38.968052782867815	21.300047420278318	17.503058900669153
6	26.077201383969605	32.748971097047644	25.051079250408343	16.122748268574405
7	76.90808076668637	6.9906857206418715	9.653392891641737	6.447840621030015
8	76.58857932358778	6.278357033715233	10.063490483745383	7.069573158951601
9	70.66411807063865	8.567849051301716	12.559494886220604	8.208537991839028
10	37.97134878492855	25.827366769508174	20.659873663011595	15.54141078255168
11	28.526224584779847	25.454297974978484	25.62787961103663	20.39159782920504
12	26.335963890336217	23.41932405613156	30.14144122519949	20.10327082833274
13	23.209152699150533	26.791871813035307	30.483189218619195	19.51578626919497
14	19.139497579224063	29.24396855040307	30.04923512847383	21.567298741899034
15	19.035729130686775	26.91774045301002	33.902571818304224	20.14395859799898
16	21.060604286558984	27.085028657069426	30.833572386176694	21.020794670194892
17	20.38793885711275	27.33252152939179	28.00123526897836	24.278304344517103
18	21.724195465216347	27.20079853406942	30.563247527998456	20.511758472715776
19	20.77432631005837	28.32102942984433	28.373425910205896	22.531218349891404
20	22.862428503685315	27.062343030097242	29.943417655564858	20.131810810652585
21	22.248013909948305	28.64828789377858	29.572983320941614	19.530714875331505
22	20.316662080754977	26.19897197520095	29.588058285961843	23.89630765808223
23	19.709858148969925	27.553816161533373	28.804160104910046	23.932165584586652
24	21.861333739235302	26.83138871163202	29.044335033047837	22.26294251608484
25	20.065217518572943	27.94664340536142	27.009361114200907	24.97877796186473
26	21.052115471304877	28.973058256690067	27.75930403423627	22.215522237768788
27	22.585078419089882	27.598748338826667	28.754837161106007	21.061336080977444
28	18.708909743403606	26.235854413891214	31.832032690720265	23.22320315198492
29	21.679116929039356	24.963995714611887	30.525926012657116	22.830961343691637
30	25.128064023230085	25.427807017030318	29.14268820288854	20.301440756851058
31	24.807391709061957	27.551181701626927	26.811922980101045	20.82950360921007
32	22.273919432361705	26.696299461984747	29.224063742221023	21.805717363432525
33	24.307429762371715	24.83637076803288	29.07755849964581	21.778640969949596
34	20.479852236071025	24.374754848869816	32.644763571859286	22.500629343199876
35	22.33978093002289	23.928652971378057	30.215352461463706	23.516213637135348
36	24.61170988156639	23.61266414148806	30.60495980985054	21.17066616709501
37	20.7254424429054	25.64983344359036	33.572532535579846	20.052191577924397
38	20.905610228729664	26.022463161468973	29.644845532834154	23.42708107696721
39	23.977683197414716	23.64837570910879	31.857352777598898	20.516588315877595
40	23.384344282929284	23.64149684157529	30.81191127139035	22.162247604105072
41	20.676997652403507	24.346653943201044	28.932809563674894	26.043538840720554
42	22.651086275634757	22.843694566572804	30.196325806583808	24.30889335120863
43	23.712919976816753	22.37622429206208	30.888310608677326	23.02254512244384
44	21.833671910217607	23.406737192134088	29.929074484963085	24.830516412685217
45	22.66469765181807	23.651888322317387	28.69819627311739	24.985217752747158
46	23.721994227605627	23.296382593830682	30.391129480777224	22.590493697786467
47	20.27348621006598	23.165391392926768	33.22815008225369	23.33297231475356
48	20.680071188961026	22.202057220469168	32.88464578222969	24.233225808340116
49	22.242159554600647	21.02547815447302	33.795583474325724	22.936778816600608
50	21.38713095607477	20.75617780848062	33.07213151223853	24.78455972320608
51	20.35456903163108	21.345857750873762	30.165297723241206	28.134275494253952
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	876.0
1	1179.0
2	1482.0
3	1645.5
4	1809.0
5	1512.0
6	1215.0
7	1253.5
8	1292.0
9	1390.5
10	1489.0
11	1757.0
12	2025.0
13	2028.5
14	2032.0
15	2142.5
16	2253.0
17	2146.0
18	2039.0
19	2245.5
20	2452.0
21	2395.0
22	2338.0
23	3056.5
24	3775.0
25	4005.0
26	4959.5
27	5684.0
28	7534.5
29	9385.0
30	9547.0
31	9709.0
32	11387.0
33	13065.0
34	15028.5
35	16992.0
36	17959.0
37	18926.0
38	21114.0
39	23302.0
40	27394.0
41	31486.0
42	36850.5
43	42215.0
44	47014.0
45	51813.0
46	58603.0
47	65393.0
48	72499.5
49	79606.0
50	78589.5
51	77573.0
52	67158.0
53	56743.0
54	48187.5
55	39632.0
56	34162.0
57	28692.0
58	26553.5
59	24415.0
60	21279.0
61	18143.0
62	16865.5
63	15588.0
64	12692.0
65	9796.0
66	7742.0
67	5688.0
68	4833.5
69	3979.0
70	3291.5
71	2604.0
72	1911.5
73	1219.0
74	1029.0
75	705.0
76	571.0
77	477.0
78	383.0
79	380.5
80	378.0
81	245.0
82	112.0
83	59.5
84	7.0
85	4.0
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	683252.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.09438472370604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.56829653557966	20.24046783979021
2	13.439195612591389	7.820102550597524
3	5.299315395292923	4.62540962648732
4	2.679323565929591	3.1181308250578996
5	1.5062310791605102	2.191143324994941
6	0.9796633294886798	1.7101621084710268
7	0.6376159883936822	1.2985731410618313
8	0.43553352795443234	1.013726401790339
9	0.33448306083870344	0.8758420970043611
>10	3.9550810033442336	29.477032577302705
>50	1.062612426349724	20.281132124236066
>100	0.09519355789209594	4.463891228746236
>500	0.0057345516802467435	1.1321983135216578
>1k	0.0017203655040740227	1.7521878409378795
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	3832	0.5608472423059135	No Hit
CTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT	3716	0.5438696117976969	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	2954	0.43234414242475694	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	944	0.13816278620479705	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCC	862	0.12616135774209222	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTG	811	0.11869705467382459	No Hit
ACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	744	0.1088910094664926	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	696	0.10186578304929952	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.573580465187076	0.0
2	0.0	0.0	0.0	1.8856878574815734	0.0
3	0.0	0.0	0.0	2.5557188270213627	0.0
4	0.0	0.0	0.0	3.3080034891957872	0.0
5	0.0	0.0	0.0	5.117877444925152	0.0
6	0.0	0.0	0.0	6.2271314244232	0.0
7	0.0	0.0	0.0	7.181976781626691	0.0
8	0.0	0.0	0.0	8.769531593028633	0.0
9	0.0	0.0	0.0	9.331256988636696	0.0
10	0.0	0.0	0.0	10.557012639553196	0.0
11	0.0	0.0	0.0	12.504756663719974	0.0
12	0.0	0.0	0.0	13.997324559606119	0.0
13	0.0	0.0	0.0	14.650670616405074	0.0
14	0.0	0.0	0.0	14.933435979697096	0.0
15	0.0	0.0	0.0	15.446862943686956	0.0
16	0.0	0.0	0.0	16.42527208116478	0.0
17	0.0	0.0	0.0	17.627171234039565	0.0
18	0.0	0.0	0.0	18.83624197221523	0.0
19	0.0	0.0	0.0	19.654680849818224	0.0
20	0.0	0.0	0.0	20.365545947907947	0.0
21	0.0	0.0	0.0	21.174764215838373	0.0
22	0.0	0.0	0.0	21.978713563955903	0.0
23	0.0	0.0	0.0	22.744024166778875	0.0
24	0.0	0.0	0.0	23.363268603677707	0.0
25	0.0	0.0	0.0	23.944898807467816	0.0
26	0.0	0.0	0.0	24.48789026596336	0.0
27	0.0	0.0	0.0	24.989901237025286	0.0
28	0.0	0.0	0.0	25.534795361008822	0.0
29	0.0	0.0	0.0	26.11232751605557	0.0
30	0.0	0.0	0.0	26.711081709237586	0.0
31	0.0	0.0	0.0	27.248218812385474	0.0
32	0.0	0.0	0.0	27.83043445171035	0.0
33	1.463588836915223E-4	0.0	0.0	28.414406397639524	0.0
34	1.463588836915223E-4	0.0	0.0	28.991792193802578	0.0
35	1.463588836915223E-4	0.0	0.0	29.586301979357543	0.0
36	1.463588836915223E-4	0.0	0.0	30.153881730313266	0.0
37	1.463588836915223E-4	0.0	0.0	30.744732543775942	0.0
38	1.463588836915223E-4	0.0	0.0	31.34538940244595	0.0
39	1.463588836915223E-4	0.0	0.0	31.93463026818802	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGCG	40	6.8084773E-9	45.0	1
GTACCGG	45	3.8380676E-10	45.0	2
TCCGCTT	20	7.0312765E-4	45.0	31
TCCGCTA	25	3.8891383E-5	45.0	27
GTCGATT	20	7.0312765E-4	45.0	32
CAACGAA	20	7.0312765E-4	45.0	14
CGACGGA	20	7.0312765E-4	45.0	21
GACGTTC	25	3.8891383E-5	45.0	42
CGCATCG	20	7.0312765E-4	45.0	21
CGCGATT	25	3.8891383E-5	45.0	27
CGATTAT	25	3.8891383E-5	45.0	34
CACGACG	20	7.0312765E-4	45.0	26
CGCGAGG	70	0.0	45.0	2
CGTTCTA	20	7.0312765E-4	45.0	44
TAACGCC	35	1.2109558E-7	45.0	12
ATCCGTT	25	3.8891383E-5	45.0	40
GTGGACG	35	1.2109558E-7	45.0	12
ACGATTC	25	3.8891383E-5	45.0	24
ACGATCT	20	7.0312765E-4	45.0	12
TAATTCG	20	7.0312765E-4	45.0	26
>>END_MODULE