SRR2935139.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935139.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	525612
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	3107	0.5911204462607398	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT	2806	0.5338538693941538	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	2085	0.39668044108582	TruSeq Adapter, Index 16 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	999	0.19006415378644323	No Hit
TCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	981	0.18663957443893975	TruSeq Adapter, Index 16 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	795	0.15125225451473712	TruSeq Adapter, Index 13 (95% over 21bp)
TGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCTT	724	0.1377441915329178	TruSeq Adapter, Index 16 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCC	657	0.12499714618387708	No Hit
TGTCAAGGGAGGAAGGACAAACTTGAATAGCGACAAGCCCACCCACGTGAC	606	0.11529417136595053	No Hit
GCCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG	600	0.11415264491678272	No Hit
AAAACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTC	556	0.10578145095621866	No Hit
GATGAAGGGGACAATTAGAAGGAAAAGAGAAGATGTAGAAGGAGGAAATGA	543	0.10330814364968838	No Hit
ACCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG	531	0.10102509075135271	No Hit
GGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG	526	0.10007381871037953	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAA	20	7.0297875E-4	45.000004	13
CGTGGCT	20	7.0297875E-4	45.000004	45
AGACTCC	20	7.0297875E-4	45.000004	37
TCCATTG	35	1.2102464E-7	45.000004	12
TTGGCGT	20	7.0297875E-4	45.000004	27
TCCATGC	20	7.0297875E-4	45.000004	23
AGTCCGC	40	6.8030204E-9	45.000004	28
ACACGAT	20	7.0297875E-4	45.000004	21
GCGACTC	20	7.0297875E-4	45.000004	17
TCCGCTG	20	7.0297875E-4	45.000004	38
TCCGCGA	40	6.8030204E-9	45.000004	30
TCACCGT	20	7.0297875E-4	45.000004	44
GATCGCC	20	7.0297875E-4	45.000004	36
GATCGAA	20	7.0297875E-4	45.000004	9
AGATACG	20	7.0297875E-4	45.000004	1
CGAAACT	20	7.0297875E-4	45.000004	15
CATGCGT	20	7.0297875E-4	45.000004	21
CTATCCG	20	7.0297875E-4	45.000004	35
CGTGCAG	35	1.2102464E-7	45.000004	24
GCGCGAG	35	1.2102464E-7	45.000004	1