##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935139.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	525612
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.008418757562616	33.0	31.0	34.0	30.0	34.0
2	32.31792843390181	34.0	31.0	34.0	30.0	34.0
3	32.37097326545056	34.0	31.0	34.0	30.0	34.0
4	35.901046779753884	37.0	35.0	37.0	35.0	37.0
5	30.358564872948108	37.0	35.0	37.0	0.0	37.0
6	32.858928258867756	37.0	35.0	37.0	17.0	37.0
7	35.08271120141854	37.0	35.0	37.0	32.0	37.0
8	35.62949285784951	37.0	35.0	37.0	35.0	37.0
9	37.510992899705485	39.0	37.0	39.0	35.0	39.0
10	37.37181989756703	39.0	37.0	39.0	34.0	39.0
11	37.23765629399633	39.0	37.0	39.0	34.0	39.0
12	37.070247635137704	39.0	37.0	39.0	34.0	39.0
13	36.97434229051087	39.0	37.0	39.0	33.0	39.0
14	38.13162941485354	40.0	37.0	41.0	33.0	41.0
15	38.28760568632375	40.0	37.0	41.0	34.0	41.0
16	38.372771169608	40.0	37.0	41.0	34.0	41.0
17	38.349628623395205	40.0	37.0	41.0	34.0	41.0
18	38.27478824684368	40.0	37.0	41.0	34.0	41.0
19	38.22661202560063	40.0	37.0	41.0	34.0	41.0
20	38.16242779845209	40.0	36.0	41.0	34.0	41.0
21	38.10914705143718	40.0	36.0	41.0	34.0	41.0
22	38.11173641393271	40.0	36.0	41.0	34.0	41.0
23	38.00270161259636	40.0	36.0	41.0	34.0	41.0
24	37.96418460765736	40.0	36.0	41.0	34.0	41.0
25	37.918093574728125	40.0	36.0	41.0	34.0	41.0
26	37.80754815339071	40.0	36.0	41.0	33.0	41.0
27	37.78436945884036	40.0	36.0	41.0	33.0	41.0
28	37.732254971347686	40.0	36.0	41.0	33.0	41.0
29	37.72629430073895	40.0	36.0	41.0	33.0	41.0
30	37.57686087836655	39.0	36.0	41.0	33.0	41.0
31	32.97454015509539	38.0	33.0	40.0	9.0	41.0
32	33.84716102372092	38.0	33.0	40.0	16.0	41.0
33	35.79753125879927	38.0	34.0	40.0	29.0	41.0
34	36.68039352221791	38.0	35.0	40.0	31.0	41.0
35	36.80817218784959	39.0	35.0	40.0	32.0	41.0
36	36.986805856791705	39.0	35.0	41.0	32.0	41.0
37	37.058527963592915	39.0	35.0	41.0	32.0	41.0
38	37.041692351011775	39.0	35.0	41.0	32.0	41.0
39	37.081440301971796	39.0	35.0	41.0	32.0	41.0
40	37.037267033477164	39.0	35.0	41.0	32.0	41.0
41	36.99525124997146	39.0	35.0	41.0	32.0	41.0
42	36.91567544119997	39.0	35.0	41.0	32.0	41.0
43	36.9106983858816	39.0	35.0	41.0	32.0	41.0
44	36.84790491845696	39.0	35.0	41.0	32.0	41.0
45	36.842728096009985	39.0	35.0	41.0	32.0	41.0
46	36.785288387631944	39.0	35.0	41.0	32.0	41.0
47	36.67502073773049	39.0	35.0	41.0	31.0	41.0
48	36.694015737844644	39.0	35.0	41.0	32.0	41.0
49	36.70158786329079	39.0	35.0	41.0	32.0	41.0
50	36.50183024740683	39.0	35.0	41.0	31.0	41.0
51	35.90402235869805	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	2.0
11	5.0
12	7.0
13	9.0
14	12.0
15	14.0
16	43.0
17	79.0
18	123.0
19	284.0
20	407.0
21	730.0
22	1238.0
23	1938.0
24	2506.0
25	2896.0
26	3322.0
27	3678.0
28	4342.0
29	5557.0
30	7406.0
31	10611.0
32	17666.0
33	19631.0
34	35869.0
35	57434.0
36	59357.0
37	58690.0
38	93641.0
39	137973.0
40	137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.475841495247444	22.40949597802181	25.467074572117838	10.647587954612908
2	30.646941089625045	25.514257665350105	25.488763574652022	18.350037670372824
3	30.845186182963857	25.82456260511556	26.263289270412393	17.066961941508186
4	26.526220862537386	26.878952535330242	26.25073247947155	20.34409412266082
5	21.87126625723918	41.523405097296106	20.005631530482564	16.599697114982153
6	25.63544972337009	33.935869044085756	24.804418468375914	15.624262764168245
7	78.89393697251965	6.7070386520855685	8.914941059184342	5.4840833162104365
8	78.83324581630556	6.171662747425858	8.54775005136869	6.447341384899888
9	73.96577703705395	8.254758262748949	10.605731984810088	7.173732715387016
10	38.30487127386741	28.546912931972635	18.75775286713393	14.390462927026018
11	27.4978501251874	27.027731482538453	26.867347016430372	18.607071375843777
12	25.585222559606706	24.472234271668075	30.85736246508832	19.085180703636905
13	21.793642458695768	29.826183572673383	30.23028393567879	18.14989003295206
14	16.700341696917118	34.695174387190555	28.126831198678875	20.477652717213456
15	16.06051612215855	28.83020174577445	36.737555459159985	18.37172667290701
16	17.809905405508246	29.199105043263852	32.21425690433247	20.77673264689543
17	17.48552163953639	29.107212164105846	27.395873762395073	26.011392433962694
18	20.213769853047495	28.292352533808206	31.090424115126748	20.403453498017548
19	19.63025958311454	30.54287192834258	27.648912125293947	22.177956363248935
20	23.234629346361952	30.235040295883657	27.39321020068035	19.13712015707404
21	21.33874416870239	30.24930937649825	28.99382053682184	19.41812591797752
22	20.361026764990147	27.09165696369185	26.569598867605766	25.97771740371224
23	17.594537415431915	29.624513899987065	27.76002069967961	25.02092798490141
24	20.896783178466244	27.002617900656755	31.115918205824826	20.984680715052168
25	17.87611393955998	30.935557026856316	27.182218061992497	24.006110971591212
26	17.658462896585313	33.93567878967756	27.442295837994568	20.963562475742563
27	19.924202643775256	30.867255694314437	29.080196038142205	20.1283456237681
28	15.86189052000335	31.651484364892735	30.139532582969945	22.34709253213397
29	17.06258609011971	29.5025608243343	28.879287383088663	24.555565702457326
30	20.003348477584225	30.049161739077494	29.19948555208024	20.748004231258037
31	22.904157439327868	32.41364352412045	21.785271264735204	22.896927771816472
32	20.280929659140202	33.10293524500963	23.306165003843137	23.30997009200703
33	21.324094579271403	29.959171404001435	24.68893404260177	24.0277999741254
34	18.782485940199233	29.0518481313212	25.614331484060486	26.551334444419076
35	18.10803406314924	29.606629985616767	25.93491016186845	26.350425789365538
36	21.62964315883199	27.228449883183792	27.285145696825797	23.85676126115842
37	18.904248761443803	28.514569682579545	29.50731718453917	23.073864371437487
38	19.38121656278776	30.329786991164585	24.73916120636515	25.549835239682505
39	21.71221357198846	26.155795529782427	26.7528138626972	25.379177035531914
40	21.400196342549258	26.801328736786832	27.424221669216074	24.374253251447836
41	18.06446580367267	27.936386536076043	27.03420013241707	26.96494752783422
42	20.736398712358163	26.783254568008342	27.078529409526418	25.401817310107077
43	22.01110324726224	26.3483329908754	27.544462455195085	24.096101306667276
44	19.16622908152782	27.024497157599143	26.608981530102056	27.20029223077099
45	19.811762288532226	27.11011164128673	25.583129761116563	27.49499630906448
46	23.136838580549913	26.29905709915299	27.390546638965624	23.173557681331477
47	17.880489790948456	27.456184409792776	30.14295716231745	24.520368636941317
48	18.520886128931608	25.05212970784533	29.149638897133244	27.277345266089814
49	20.13766808976964	22.641416101611075	31.051231707038653	26.169684101580632
50	19.085180703636905	24.092105964095186	30.59214782006499	26.23056551220292
51	18.26137911615412	24.805369740416886	26.57283319254507	30.36041795088392
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1234.0
1	1168.0
2	1102.0
3	1195.0
4	1288.0
5	1119.0
6	950.0
7	963.0
8	976.0
9	1076.5
10	1177.0
11	1371.0
12	1565.0
13	1661.0
14	1757.0
15	1900.0
16	2043.0
17	2052.0
18	2061.0
19	2129.5
20	2198.0
21	2285.0
22	2372.0
23	2427.0
24	2482.0
25	2673.5
26	4026.5
27	5188.0
28	5545.0
29	5902.0
30	7152.0
31	8402.0
32	10166.0
33	11930.0
34	12111.0
35	12292.0
36	13784.0
37	15276.0
38	17477.5
39	19679.0
40	22472.5
41	25266.0
42	30135.5
43	35005.0
44	39851.5
45	44698.0
46	51808.5
47	58919.0
48	65138.5
49	71358.0
50	65992.0
51	60626.0
52	50627.0
53	40628.0
54	33405.0
55	26182.0
56	22676.0
57	19170.0
58	16093.0
59	13016.0
60	11136.5
61	9257.0
62	8139.5
63	7022.0
64	5929.0
65	4836.0
66	3709.0
67	2582.0
68	2159.0
69	1736.0
70	1495.0
71	1254.0
72	955.5
73	657.0
74	435.0
75	188.0
76	163.0
77	133.5
78	104.0
79	88.0
80	72.0
81	87.5
82	103.0
83	53.5
84	4.0
85	3.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	525612.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.063482656106903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.47737817933296	19.778406801835978
2	12.382098781385032	6.949696287952026
3	5.270211044063531	4.437014286873
4	2.9685197286349148	3.332280076754284
5	1.7166548201431522	2.408765638580483
6	1.0665491450080553	1.7958650059691526
7	0.7778640731894143	1.528070244873186
8	0.526301608301174	1.1815884845152933
9	0.40020205082246507	1.0107956980975197
>10	2.8590314796404854	17.95905977252805
>50	1.278778528653504	26.004847272670773
>100	0.26577938540898244	10.3070643064706
>500	0.00835306639856802	1.602127723112227
>1k	0.002278109017791278	1.7044183997674371
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	3107	0.5911204462607398	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT	2806	0.5338538693941538	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	2085	0.39668044108582	TruSeq Adapter, Index 16 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	999	0.19006415378644323	No Hit
TCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	981	0.18663957443893975	TruSeq Adapter, Index 16 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	795	0.15125225451473712	TruSeq Adapter, Index 13 (95% over 21bp)
TGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCTT	724	0.1377441915329178	TruSeq Adapter, Index 16 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCC	657	0.12499714618387708	No Hit
TGTCAAGGGAGGAAGGACAAACTTGAATAGCGACAAGCCCACCCACGTGAC	606	0.11529417136595053	No Hit
GCCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG	600	0.11415264491678272	No Hit
AAAACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTC	556	0.10578145095621866	No Hit
GATGAAGGGGACAATTAGAAGGAAAAGAGAAGATGTAGAAGGAGGAAATGA	543	0.10330814364968838	No Hit
ACCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG	531	0.10102509075135271	No Hit
GGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG	526	0.10007381871037953	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5738072951150278	0.0
2	0.0	0.0	0.0	1.9870170391847979	0.0
3	0.0	0.0	0.0	2.774099525886015	0.0
4	0.0	0.0	0.0	3.6941698439152835	0.0
5	0.0	0.0	0.0	6.03106473977002	0.0
6	0.0	0.0	0.0	7.4570215291888315	0.0
7	0.0	0.0	0.0	8.68187940914591	0.0
8	0.0	0.0	0.0	10.752798642344542	0.0
9	0.0	0.0	0.0	11.438285275069823	0.0
10	0.0	0.0	0.0	12.881174706817957	0.0
11	0.0	0.0	0.0	15.015829166761794	0.0
12	0.0	0.0	0.0	16.740104868229796	0.0
13	0.0	0.0	0.0	17.42083514075021	0.0
14	0.0	0.0	0.0	17.674254012465468	0.0
15	0.0	0.0	0.0	18.169676491404307	0.0
16	0.0	0.0	0.0	19.223876167210793	0.0
17	0.0	0.0	0.0	20.521411231098224	0.0
18	0.0	0.0	0.0	21.97172058476595	0.0
19	0.0	0.0	0.0	22.838329414092524	0.0
20	0.0	0.0	0.0	23.555398278578114	0.0
21	0.0	0.0	0.0	24.371399435324918	0.0
22	0.0	0.0	0.0	25.171609476191563	0.0
23	0.0	0.0	0.0	26.00206996796116	0.0
24	0.0	0.0	0.0	26.631241295860825	0.0
25	0.0	0.0	0.0	27.17879348264499	0.0
26	0.0	0.0	0.0	27.667557057297017	0.0
27	0.0	0.0	0.0	28.13691468231319	0.0
28	0.0	0.0	0.0	28.606272307329363	0.0
29	0.0	0.0	0.0	29.146594826602133	0.0
30	0.0	0.0	0.0	29.781854295564028	0.0
31	5.707632245839136E-4	0.0	0.0	30.354710318638084	0.0
32	5.707632245839136E-4	0.0	0.0	30.97893503192469	0.0
33	5.707632245839136E-4	0.0	0.0	31.529531289239973	0.0
34	5.707632245839136E-4	0.0	0.0	32.09515764480263	0.0
35	5.707632245839136E-4	0.0	0.0	32.7205238845384	0.0
36	5.707632245839136E-4	0.0	0.0	33.30574644414511	0.0
37	5.707632245839136E-4	0.0	0.0	33.87137279970777	0.0
38	7.610176327785515E-4	0.0	0.0	34.423871601105	0.0
39	7.610176327785515E-4	0.0	0.0	34.95905725135651	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAA	20	7.0297875E-4	45.000004	13
CGTGGCT	20	7.0297875E-4	45.000004	45
AGACTCC	20	7.0297875E-4	45.000004	37
TCCATTG	35	1.2102464E-7	45.000004	12
TTGGCGT	20	7.0297875E-4	45.000004	27
TCCATGC	20	7.0297875E-4	45.000004	23
AGTCCGC	40	6.8030204E-9	45.000004	28
ACACGAT	20	7.0297875E-4	45.000004	21
GCGACTC	20	7.0297875E-4	45.000004	17
TCCGCTG	20	7.0297875E-4	45.000004	38
TCCGCGA	40	6.8030204E-9	45.000004	30
TCACCGT	20	7.0297875E-4	45.000004	44
GATCGCC	20	7.0297875E-4	45.000004	36
GATCGAA	20	7.0297875E-4	45.000004	9
AGATACG	20	7.0297875E-4	45.000004	1
CGAAACT	20	7.0297875E-4	45.000004	15
CATGCGT	20	7.0297875E-4	45.000004	21
CTATCCG	20	7.0297875E-4	45.000004	35
CGTGCAG	35	1.2102464E-7	45.000004	24
GCGCGAG	35	1.2102464E-7	45.000004	1
>>END_MODULE