##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935136.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	402609
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.955997009505502	33.0	31.0	34.0	30.0	34.0
2	32.27561480245102	34.0	31.0	34.0	30.0	34.0
3	32.33106562446443	34.0	31.0	34.0	30.0	34.0
4	35.85619794887844	37.0	35.0	37.0	35.0	37.0
5	30.336924907292186	37.0	35.0	37.0	0.0	37.0
6	32.82875941670454	37.0	35.0	37.0	17.0	37.0
7	34.995837152174936	37.0	35.0	37.0	32.0	37.0
8	35.544036521786644	37.0	35.0	37.0	33.0	37.0
9	37.33482112918489	39.0	37.0	39.0	34.0	39.0
10	37.38182703317611	39.0	37.0	39.0	34.0	39.0
11	37.24906050287003	39.0	37.0	39.0	34.0	39.0
12	36.967236201873284	39.0	35.0	39.0	33.0	39.0
13	36.76399186307311	39.0	35.0	39.0	33.0	39.0
14	37.87532320439931	40.0	36.0	41.0	33.0	41.0
15	38.04576152048265	40.0	36.0	41.0	33.0	41.0
16	38.14435842219126	40.0	36.0	41.0	34.0	41.0
17	38.14837720965006	40.0	36.0	41.0	34.0	41.0
18	38.07323979344724	40.0	36.0	41.0	34.0	41.0
19	38.02455732484867	40.0	36.0	41.0	34.0	41.0
20	37.962241281243095	40.0	36.0	41.0	34.0	41.0
21	37.903524262994615	40.0	35.0	41.0	34.0	41.0
22	37.848490719283475	40.0	35.0	41.0	34.0	41.0
23	37.77869595562941	39.0	35.0	41.0	33.0	41.0
24	37.75850018255926	39.0	35.0	41.0	33.0	41.0
25	37.710701946553606	39.0	35.0	41.0	33.0	41.0
26	37.633935157932385	39.0	35.0	41.0	33.0	41.0
27	37.58014848152923	39.0	35.0	41.0	33.0	41.0
28	37.55988564587478	39.0	35.0	41.0	33.0	41.0
29	37.54390984801631	39.0	35.0	41.0	33.0	41.0
30	37.3756349212263	39.0	35.0	41.0	33.0	41.0
31	32.80948264941917	38.0	33.0	40.0	9.0	41.0
32	33.671994416418904	38.0	33.0	40.0	16.0	41.0
33	35.53876838322044	38.0	34.0	40.0	28.0	41.0
34	36.36684227128554	38.0	35.0	40.0	31.0	41.0
35	36.52796385575086	39.0	35.0	40.0	31.0	41.0
36	36.627472311845985	39.0	35.0	40.0	31.0	41.0
37	36.632901897374374	39.0	35.0	40.0	31.0	41.0
38	36.633691745589395	39.0	35.0	41.0	31.0	41.0
39	36.66944355441632	39.0	35.0	41.0	31.0	41.0
40	36.57785593466614	39.0	35.0	41.0	31.0	41.0
41	36.573857017602684	39.0	35.0	41.0	31.0	41.0
42	36.49351852541796	39.0	35.0	41.0	31.0	41.0
43	36.43036544140841	39.0	35.0	41.0	31.0	41.0
44	36.37018049770373	38.0	35.0	40.0	31.0	41.0
45	36.37940781254269	38.0	35.0	40.0	31.0	41.0
46	36.340076848754	38.0	35.0	40.0	31.0	41.0
47	36.23738912940347	38.0	35.0	40.0	31.0	41.0
48	36.23322379777898	38.0	35.0	40.0	31.0	41.0
49	36.204836454227305	38.0	35.0	40.0	31.0	41.0
50	36.00072278563072	38.0	35.0	40.0	31.0	41.0
51	35.42757116706283	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	7.0
12	6.0
13	10.0
14	14.0
15	12.0
16	32.0
17	55.0
18	107.0
19	212.0
20	402.0
21	643.0
22	1025.0
23	1574.0
24	2112.0
25	2510.0
26	2792.0
27	3286.0
28	3868.0
29	4875.0
30	6605.0
31	9446.0
32	15295.0
33	16357.0
34	31422.0
35	48028.0
36	44235.0
37	44455.0
38	67621.0
39	95512.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.41834881982271	22.890447059057298	24.292800210626194	11.398403910493805
2	30.77576507231582	25.54662215698109	24.869538435554098	18.80807433514899
3	30.883313587127958	26.388133399899154	25.777118742998788	16.951434269974094
4	28.889071034179565	26.57118941702744	25.4065358697893	19.1332036790037
5	21.07379616451694	39.610639603188204	20.328159579145026	18.98740465314983
6	25.115682957907055	34.16540613846188	24.222508687088464	16.496402216542602
7	75.33438149668785	8.479442834114488	9.985867181309906	6.200308487887752
8	75.15977039758177	7.4039576859931095	10.084722398158014	7.351549518267103
9	68.14179514119158	9.60510073048541	12.974623021343287	9.278481106979724
10	40.24872767374798	23.823362120568593	19.586993832726044	16.340916372957384
11	33.28787980397855	24.312918985914372	23.826839439754202	18.572361770352874
12	29.625765941645614	22.899885496846817	28.412678305750738	19.061670255756827
13	22.82065229540323	29.02269944288379	29.512007928287744	18.644640333425233
14	17.84460854079268	33.229262137706804	27.516275095688375	21.40985422581214
15	17.005332717351077	27.175746195440244	35.85488650278558	19.9640345844231
16	18.534359639252973	28.74203010861655	32.266541483175985	20.457068768954496
17	19.91261993646441	26.372733843505735	25.47856605291983	28.23608016711002
18	20.45061089046693	27.731123745370816	30.134199682570433	21.684065681591818
19	21.671895064442175	29.413897851265126	27.100238693124094	21.813968391168604
20	23.76896691330795	29.232083733845986	26.555541480692185	20.443407872153877
21	22.041981177768008	28.60840169991232	28.526187939166785	20.823429183152886
22	20.494325760228907	26.7068048652663	27.680205857295785	25.118663517209004
23	19.37959658129848	28.251231343561617	27.693369994212745	24.675802080927152
24	21.36762963570114	26.34864098914828	29.174707967283393	23.109021407867186
25	19.07781495197574	29.612601804728655	26.805660082114407	24.503923161181195
26	17.150634983321286	30.820473461845115	28.482721449346638	23.54617010548696
27	20.20744692741593	30.66846493744551	28.02545397643868	21.09863415869988
28	16.80787066359669	29.361241303597286	29.88780678027565	23.94308125253037
29	18.075353506752208	27.185929773055246	31.357470896080315	23.381245824112227
30	19.613818866443623	30.913864319972976	27.467841007031634	22.004475806551767
31	25.028998358208582	29.550506819271305	23.212844223551883	22.20765059896823
32	24.522303276876574	27.763413137808644	25.994202812157702	21.720080773157083
33	23.822368600801276	29.16477276961022	24.188480635057836	22.82437799453067
34	22.156484330951372	26.01009912843478	27.55750616603206	24.27591037458179
35	22.4294538870219	26.483511297561652	27.288510689030797	23.79852412638565
36	25.894602455484105	28.00806738051062	25.963900459254514	20.133429704750764
37	22.95303880439831	27.401523562563177	29.0261767620694	20.619260870969104
38	22.260803906519726	29.26611178587662	24.913004925374246	23.56007938222941
39	25.222486332893702	26.47605989930677	27.344892935826078	20.95656083197345
40	25.025521039022973	25.075942167214343	29.064924032994792	20.833612760767892
41	22.16840656815918	25.827539871190165	25.9097536319357	26.09429992871496
42	24.32732502254048	24.25504645946812	26.914202116693865	24.50342640129754
43	24.337260220213654	23.36634302760246	28.125302713054108	24.171094039129777
44	21.11204667555867	24.685985658542158	27.76987101629621	26.432096649602965
45	20.96128005086821	25.302961434046434	27.4531865904637	26.282571924621656
46	24.78782143469222	25.27886857968898	27.8888450084325	22.0444649771863
47	21.979389432426995	24.30621272748498	31.521898417571393	22.192499422516633
48	22.58643001025809	22.46174327945972	30.062169499439907	24.88965721084228
49	23.43961511044214	21.39221924994225	31.025386913854387	24.14277872576122
50	21.397683608662497	22.34798526610185	31.228561706270845	25.0257694189648
51	20.407144400646782	22.19026400304017	27.58085388056402	29.821737715749023
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1086.0
1	1083.0
2	1080.0
3	1121.0
4	1162.0
5	999.5
6	837.0
7	863.0
8	889.0
9	941.5
10	994.0
11	1110.5
12	1227.0
13	1276.0
14	1325.0
15	1396.0
16	1467.0
17	1506.5
18	1546.0
19	1567.0
20	1588.0
21	1743.5
22	1899.0
23	2180.0
24	2461.0
25	2295.5
26	2659.5
27	3189.0
28	4103.0
29	5017.0
30	4939.0
31	4861.0
32	5618.0
33	6375.0
34	7572.0
35	8769.0
36	9475.5
37	10182.0
38	11392.0
39	12602.0
40	14586.0
41	16570.0
42	19733.0
43	22896.0
44	27658.5
45	32421.0
46	36683.5
47	40946.0
48	45440.0
49	49934.0
50	48624.5
51	47315.0
52	38522.0
53	29729.0
54	25058.5
55	20388.0
56	17887.0
57	15386.0
58	14558.0
59	13730.0
60	12962.5
61	12195.0
62	11201.5
63	10208.0
64	8751.0
65	7294.0
66	5952.5
67	4611.0
68	3951.0
69	3291.0
70	2748.5
71	2206.0
72	1521.5
73	837.0
74	812.5
75	777.0
76	766.0
77	454.0
78	142.0
79	119.5
80	97.0
81	79.5
82	62.0
83	53.0
84	44.0
85	27.0
86	10.0
87	32.0
88	54.0
89	28.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	402609.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.29600030673847
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.6966625675002	22.125227733978726
2	12.193076327178447	7.6318904095092455
3	5.579038042859524	5.238047289019118
4	3.068348562726628	3.8410815024109235
5	1.716490733508907	2.685964726120425
6	1.1103196798951502	2.084913902554583
7	0.7672927147910258	1.6809235126220696
8	0.4734154158215188	1.185280719901198
9	0.33301968017094713	0.9379965611501919
>10	2.8453495489634797	21.36540251682048
>50	1.1125103342281824	23.554052441509853
>100	0.09713039601845465	5.0925471702448855
>500	0.00489733089168679	0.9678802782934325
>1k	0.002448665445843395	1.6087912358648822
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	2216	0.5504099510939895	No Hit
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT	2188	0.5434553127227657	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	1894	0.470431609824917	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	794	0.1972136738125576	No Hit
TCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	740	0.18380115695376906	No Hit
ACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	649	0.16119858224729203	No Hit
TGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCTT	569	0.14132818690093862	Illumina Single End Adapter 2 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTG	520	0.12915756975129716	No Hit
AAAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTC	517	0.12841242992580892	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCC	500	0.12418997091470882	No Hit
GCAATTGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	468	0.11624181277616744	No Hit
GTGGCATGCCTGTGTATGTGTGTAACAGGATTCTGAATGTTAGGTTGTAAT	460	0.11425477324153212	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	457	0.11350963341604386	No Hit
CGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTG	440	0.10928717440494376	No Hit
TTTTTAGGGTACCAAAGCATATTTAATTGATGATACAATAAAAGTAAAAGA	423	0.10506471539384364	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.572764145858637	0.0
2	0.0	0.0	0.0	2.012374288701942	0.0
3	0.0	0.0	0.0	2.8186155798802313	0.0
4	0.0	0.0	0.0	3.674781239366234	0.0
5	0.0	0.0	0.0	5.978505199834082	0.0
6	0.0	0.0	0.0	7.449162835406064	0.0
7	0.0	0.0	0.0	8.637164097176168	0.0
8	0.0	0.0	0.0	10.576017923096602	0.0
9	0.0	0.0	0.0	11.290358635798007	0.0
10	0.0	0.0	0.0	12.750584313813153	0.0
11	0.0	0.0	0.0	15.04238603707319	0.0
12	0.0	0.0	0.0	16.69659644965711	0.0
13	0.0	0.0	0.0	17.418388560613398	0.0
14	0.0	0.0	0.0	17.72464102888907	0.0
15	0.0	0.0	0.0	18.300137354107832	0.0
16	0.0	0.0	0.0	19.369909763567133	0.0
17	0.0	0.0	0.0	20.585977958763962	0.0
18	0.0	0.0	0.0	22.095879625144992	0.0
19	0.0	0.0	0.0	22.946829305852578	0.0
20	0.0	0.0	0.0	23.669118176692525	0.0
21	0.0	0.0	0.0	24.525035456236697	0.0
22	0.0	0.0	0.0	25.284829698292885	0.0
23	0.0	0.0	0.0	26.003144490063562	0.0
24	0.0	0.0	0.0	26.572431316736587	0.0
25	0.0	0.0	0.0	27.0791263980686	0.0
26	0.0	0.0	0.0	27.583834439865974	0.0
27	0.0	0.0	0.0	28.053272529923575	0.0
28	0.0	0.0	0.0	28.55400649265168	0.0
29	0.0	0.0	0.0	29.105658343454817	0.0
30	0.0	0.0	0.0	29.690344726521264	0.0
31	0.0	0.0	0.0	30.24274171714989	0.0
32	0.0	0.0	0.0	30.798367647022296	0.0
33	0.0	0.0	0.0	31.30804328765626	0.0
34	0.0	0.0	0.0	31.83411200445097	0.0
35	0.0	0.0	0.0	32.377815697115565	0.0
36	0.0	0.0	0.0	32.9294675479187	0.0
37	0.0	0.0	0.0	33.497264094940746	0.0
38	0.0	0.0	0.0	34.01265247423679	0.0
39	0.0	0.0	0.0	34.599325896837875	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAAAGT	25	3.8862745E-5	45.000004	13
CGGCCTT	25	3.8862745E-5	45.000004	40
CCAATAC	25	3.8862745E-5	45.000004	24
TACGGTG	25	3.8862745E-5	45.000004	40
CGACATA	25	3.8862745E-5	45.000004	21
CATTAGC	25	3.8862745E-5	45.000004	13
AAAGTCC	25	3.8862745E-5	45.000004	25
TAGCAAC	25	3.8862745E-5	45.000004	21
AATGCTC	25	3.8862745E-5	45.000004	24
CTACGGT	25	3.8862745E-5	45.000004	28
TACTCAC	65	0.0	45.000004	30
GTTCAAT	25	3.8862745E-5	45.000004	39
TACTACG	25	3.8862745E-5	45.000004	26
ACCGTTT	25	3.8862745E-5	45.000004	29
CGGTCGT	25	3.8862745E-5	45.000004	31
GAACCGC	25	3.8862745E-5	45.000004	28
GATCTAG	25	3.8862745E-5	45.000004	9
AACAGCC	25	3.8862745E-5	45.000004	32
GTAACGT	25	3.8862745E-5	45.000004	31
TACACGG	25	3.8862745E-5	45.000004	2
>>END_MODULE