##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935135.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	441541
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99390770053064	33.0	31.0	34.0	30.0	34.0
2	32.323747964515185	34.0	31.0	34.0	30.0	34.0
3	32.35563175333661	34.0	31.0	34.0	30.0	34.0
4	35.88762764952745	37.0	35.0	37.0	35.0	37.0
5	30.356177569013976	37.0	35.0	37.0	0.0	37.0
6	32.84242459930108	37.0	35.0	37.0	17.0	37.0
7	35.035047707913876	37.0	35.0	37.0	32.0	37.0
8	35.62260129863365	37.0	35.0	37.0	35.0	37.0
9	37.402143855270516	39.0	37.0	39.0	35.0	39.0
10	37.4176124074548	39.0	37.0	39.0	35.0	39.0
11	37.326255092958526	39.0	37.0	39.0	35.0	39.0
12	37.15001777864343	39.0	37.0	39.0	34.0	39.0
13	37.081317476746214	39.0	37.0	39.0	33.0	39.0
14	38.2476644298038	40.0	37.0	41.0	34.0	41.0
15	38.396939355575135	40.0	38.0	41.0	34.0	41.0
16	38.473093098942115	40.0	38.0	41.0	34.0	41.0
17	38.46229455475256	40.0	38.0	41.0	34.0	41.0
18	38.42374547324031	40.0	37.0	41.0	34.0	41.0
19	38.373960742037546	40.0	37.0	41.0	34.0	41.0
20	38.305758695115514	40.0	37.0	41.0	34.0	41.0
21	38.23629968677881	40.0	37.0	41.0	34.0	41.0
22	38.21908724218136	40.0	37.0	41.0	34.0	41.0
23	38.15034390917265	40.0	36.0	41.0	34.0	41.0
24	38.14050337341266	40.0	36.0	41.0	34.0	41.0
25	38.09012753062569	40.0	36.0	41.0	34.0	41.0
26	38.00260224984769	40.0	36.0	41.0	34.0	41.0
27	37.972007129575736	40.0	36.0	41.0	34.0	41.0
28	37.92168111228629	40.0	36.0	41.0	34.0	41.0
29	37.91614142288032	40.0	36.0	41.0	34.0	41.0
30	37.80932461538113	40.0	36.0	41.0	33.0	41.0
31	33.18736198903386	39.0	33.0	41.0	9.0	41.0
32	34.078434392276144	39.0	33.0	41.0	16.0	41.0
33	36.042211255579886	39.0	34.0	41.0	30.0	41.0
34	36.91254039828691	39.0	35.0	41.0	32.0	41.0
35	37.04124871755964	39.0	35.0	41.0	32.0	41.0
36	37.18691129476085	39.0	35.0	41.0	33.0	41.0
37	37.24997905064309	40.0	35.0	41.0	33.0	41.0
38	37.22417623731432	40.0	35.0	41.0	33.0	41.0
39	37.23884078715227	40.0	35.0	41.0	33.0	41.0
40	37.201374277813386	40.0	35.0	41.0	33.0	41.0
41	37.1117676501163	40.0	35.0	41.0	32.0	41.0
42	37.01705617371886	40.0	35.0	41.0	32.0	41.0
43	36.98174801434068	39.0	35.0	41.0	32.0	41.0
44	36.91172733675921	39.0	35.0	41.0	32.0	41.0
45	36.914379412104424	39.0	35.0	41.0	32.0	41.0
46	36.893921515782225	39.0	35.0	41.0	32.0	41.0
47	36.80503056341314	39.0	35.0	41.0	32.0	41.0
48	36.80164695917254	39.0	35.0	41.0	32.0	41.0
49	36.766984266466764	39.0	35.0	41.0	32.0	41.0
50	36.57475070265276	39.0	35.0	41.0	31.0	41.0
51	35.98153512357856	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	4.0
12	5.0
13	6.0
14	10.0
15	25.0
16	37.0
17	60.0
18	117.0
19	223.0
20	357.0
21	657.0
22	991.0
23	1524.0
24	2214.0
25	2506.0
26	2917.0
27	3276.0
28	3866.0
29	4735.0
30	6171.0
31	8616.0
32	13942.0
33	15257.0
34	27550.0
35	43160.0
36	49750.0
37	48562.0
38	73470.0
39	131398.0
40	129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.30860101326944	23.409830570660482	24.58956246418792	11.692005951882157
2	31.961244822111652	26.296765192813353	24.71842931913458	17.023560665940423
3	30.544841815369356	25.442484389898105	26.812232612599963	17.200441182132575
4	29.37326318507228	26.76127471741016	25.80553108318367	18.05993101433389
5	22.761872623380388	40.54096901533492	19.60225664207854	17.094901719206142
6	26.30922156719308	33.22839781583137	23.836291533515574	16.626089083459973
7	77.31354506150052	8.141939253659343	8.873015189982357	5.671500494857782
8	77.66390890087217	6.538237672152756	9.624021325312938	6.173832101662134
9	71.04821522803093	10.127032370719819	11.569707003426636	7.2550453978226255
10	37.399244917233055	32.742372735487756	17.508453348613155	12.349928998666035
11	27.900919733388292	25.696367947710407	27.804439451828934	18.598272867072367
12	26.42246133428153	23.796657615034615	30.875048976199267	18.90583207448459
13	22.97023379482313	27.804439451828934	31.273879435884776	17.951447317463156
14	16.88155799801151	33.11470508967457	29.439848168120285	20.56388874419363
15	15.894333708534427	30.179756806276202	35.53826258490152	18.387646900287855
16	17.921552018951807	29.64888877816556	32.330632942354164	20.09892626052847
17	17.024240104542955	28.345046099909183	28.4281640889521	26.20254970659576
18	19.47542810293948	29.030826129396818	30.390609252594892	21.103136515068815
19	20.59808715385434	30.05632546014979	28.54434808998485	20.801239296011016
20	23.463732699794583	29.25164367521929	28.23860071884604	19.046022906140088
21	21.244686223929374	29.09786407151318	30.075802700089007	19.58164700446844
22	20.156678541743574	27.023990977055355	27.67670499455317	25.1426254866479
23	18.557280071386348	28.279367034997883	29.023352304768977	24.14000058884679
24	20.583818943201198	27.482838513297747	29.68716381944146	22.2461787240596
25	18.699735698383616	29.958259821851197	28.150726659585406	23.191277820179778
26	17.993346031285885	31.45959265390983	29.150180843908043	21.39688047089625
27	20.375457771758455	30.049078115056133	29.655003725588337	19.920460387597075
28	18.353222011092967	28.641734289680915	30.798498893647476	22.206544805578645
29	18.585137054090104	25.55730951372579	32.54737385656145	23.31017957562265
30	19.37962725998265	26.626972353643264	32.16145273032402	21.831947656050062
31	23.586484607318457	28.954276046845028	26.72277319660009	20.736466149236424
32	20.484847386765896	28.23814775977769	28.45081204236979	22.826192811086628
33	21.289076212628046	27.517036922958454	28.77241298090098	22.421473883512515
34	19.412013833369947	25.9110705461101	31.910513406456026	22.766402214063927
35	19.546542676671024	24.661582956056176	30.20738730944578	25.584487057827022
36	23.50676381128819	24.956459309554493	31.254855155013917	20.281921724143398
37	21.928427937609417	26.15227125000849	31.402972770365604	20.516328042016482
38	20.58268654553031	25.576107315062472	27.866494844193408	25.974711295213808
39	22.091493202216782	24.585032873504385	30.662837652675517	22.660636271603316
40	21.39076552347347	23.353663646184614	33.13214401380619	22.123426816535723
41	19.386648125542134	23.960175838710335	30.902679479368842	25.750496556378682
42	22.784747056332254	24.607680826922078	29.109188048222023	23.49838406852365
43	23.83493265631051	23.547303647905856	30.150087987299028	22.467675708484602
44	21.387368330460816	24.382333690416065	29.327061360100192	24.903236619022923
45	20.10300289214365	24.047143979834264	29.551502578469496	26.29835054955259
46	21.579649454977	23.063996321972365	32.675561272905576	22.680792950145058
47	19.69035718087335	23.704933403692976	33.85597260503555	22.74873681039813
48	19.93993762753629	22.528372223644013	31.703058153149993	25.82863199566971
49	20.946412677418405	21.13688196566117	33.72665279102054	24.190052565899883
50	20.137880740406892	22.37821629248473	31.957847629098996	25.526055338009378
51	18.5695099662319	22.72971252952727	29.79247680283371	28.908300701407118
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1188.0
1	1197.0
2	1206.0
3	1276.0
4	1346.0
5	1154.5
6	963.0
7	986.0
8	1009.0
9	1109.0
10	1209.0
11	1343.5
12	1478.0
13	1518.0
14	1558.0
15	1640.5
16	1723.0
17	1839.0
18	1955.0
19	1823.5
20	1692.0
21	1701.0
22	1710.0
23	2013.5
24	2317.0
25	3601.0
26	5254.0
27	5623.0
28	5749.5
29	5876.0
30	7277.5
31	8679.0
32	9436.0
33	10193.0
34	11136.5
35	12080.0
36	12345.5
37	12611.0
38	14474.0
39	16337.0
40	19526.0
41	22715.0
42	26460.0
43	30205.0
44	32920.5
45	35636.0
46	40435.5
47	45235.0
48	50045.5
49	54856.0
50	51253.5
51	47651.0
52	38945.0
53	30239.0
54	25255.5
55	20272.0
56	17220.0
57	14168.0
58	12855.5
59	11543.0
60	11751.0
61	11959.0
62	10433.0
63	8907.0
64	7364.5
65	5822.0
66	4492.0
67	3162.0
68	2292.0
69	1422.0
70	1190.0
71	958.0
72	690.5
73	423.0
74	373.5
75	213.5
76	103.0
77	162.5
78	222.0
79	140.5
80	59.0
81	38.0
82	17.0
83	11.0
84	5.0
85	2.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	441541.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.572604416524857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.90536674479532	21.677617284972442
2	12.120059307881153	7.410835574493412
3	5.430670652006513	4.980892366806787
4	2.858674284569357	3.4958847223132463
5	1.714540096902278	2.6208978069431774
6	1.121021868034955	2.0563534868223843
7	0.6938811900728243	1.4849628595314768
8	0.49894131502339656	1.2203148361016811
9	0.3794975260868175	1.0442004967891887
>10	3.0398709817280394	23.134260660543294
>50	1.1290495614453038	23.23082050246862
>100	0.10135517983746987	5.059995593929352
>500	0.00471419441104511	1.0122393274539894
>1k	0.002357097205522555	1.5707244808309533
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGCT	2488	0.5634810810321125	No Hit
CCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGC	2182	0.49417834357398294	No Hit
GCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGC	1869	0.4232902493766151	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	963	0.218099791412349	No Hit
ACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGC	744	0.16850077342760922	No Hit
TCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGC	724	0.16397118274407133	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCC	712	0.16125342833394862	No Hit
TGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGCTT	541	0.12252542798969972	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTG	530	0.12003415311375389	No Hit
AAAACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTC	461	0.10440706525554819	No Hit
ACCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTG	447	0.10123635177707166	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5861290344498019	0.0
2	0.0	0.0	0.0	1.9024280870859105	0.0
3	0.0	0.0	0.0	2.6887650297480867	0.0
4	0.0	0.0	0.0	3.5240215517924725	0.0
5	0.0	0.0	0.0	5.507755791647888	0.0
6	0.0	0.0	0.0	6.792347709499231	0.0
7	0.0	0.0	0.0	7.820564794662331	0.0
8	0.0	0.0	0.0	9.47590370996125	0.0
9	0.0	0.0	0.0	10.10506385590466	0.0
10	0.0	0.0	0.0	11.386938019345882	0.0
11	0.0	0.0	0.0	13.493197687190998	0.0
12	0.0	0.0	0.0	15.12203849699122	0.0
13	0.0	0.0	0.0	15.799212304180132	0.0
14	0.0	0.0	0.0	16.081858762832898	0.0
15	0.0	0.0	0.0	16.570601597586634	0.0
16	0.0	0.0	0.0	17.565752670759906	0.0
17	0.0	0.0	0.0	18.80074557062651	0.0
18	0.0	0.0	0.0	20.223037045257406	0.0
19	0.0	0.0	0.0	21.09679508811186	0.0
20	0.0	0.0	0.0	21.838968521609544	0.0
21	0.0	0.0	0.0	22.659503873932433	0.0
22	0.0	0.0	0.0	23.544585893495736	0.0
23	0.0	0.0	0.0	24.290609479074423	0.0
24	0.0	0.0	0.0	24.92248737942796	0.0
25	0.0	0.0	0.0	25.44882581685506	0.0
26	0.0	0.0	0.0	25.96723747058597	0.0
27	0.0	0.0	0.0	26.40774016456003	0.0
28	0.0	0.0	0.0	26.893538765369467	0.0
29	0.0	0.0	0.0	27.44026036087249	0.0
30	0.0	0.0	0.0	28.05356693942352	0.0
31	0.0	0.0	0.0	28.593947107969587	0.0
32	0.0	0.0	0.0	29.187097007978874	0.0
33	0.0	0.0	0.0	29.73449804208443	0.0
34	0.0	0.0	0.0	30.269895660878603	0.0
35	0.0	0.0	0.0	30.86531035622966	0.0
36	0.0	0.0	0.0	31.472501987357912	0.0
37	0.0	0.0	0.0	32.04436281115457	0.0
38	0.0	0.0	0.0	32.62505633678413	0.0
39	0.0	0.0	0.0	33.20439098520862	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAA	30	2.1624528E-6	45.000004	10
GGTCGAA	30	2.1624528E-6	45.000004	9
CGAAAGG	55	1.8189894E-12	45.000004	2
ACGCATC	55	1.8189894E-12	45.000004	29
CGGCCGG	65	0.0	45.000004	21
GGCGTAA	30	2.1624528E-6	45.000004	32
GGTCCTT	30	2.1624528E-6	45.000004	9
TACGGGT	45	3.8380676E-10	45.000004	4
CGCGAGG	35	1.2096461E-7	45.000004	2
GTGGCGT	70	0.0	45.000004	34
TCGGCGT	60	0.0	45.000004	4
TAGCACG	45	3.8380676E-10	45.000004	1
CTAACGG	70	0.0	45.000004	2
GGGACGT	35	1.2096461E-7	45.000004	7
TCTACGG	30	2.1624528E-6	45.000004	2
GCCCTAC	30	2.1624528E-6	45.000004	26
ACGTGCG	35	1.2096461E-7	45.000004	1
GGAACGT	55	1.8189894E-12	45.000004	8
GCGATCA	30	2.1624528E-6	45.000004	9
ACGTCGT	45	3.8380676E-10	45.000004	31
>>END_MODULE