##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935133.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1040626
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05031778948441	33.0	31.0	34.0	30.0	34.0
2	32.36624877717835	34.0	31.0	34.0	30.0	34.0
3	32.422284278885975	34.0	31.0	34.0	30.0	34.0
4	35.930253520477095	37.0	35.0	37.0	35.0	37.0
5	30.418939176995387	37.0	35.0	37.0	0.0	37.0
6	32.89937114775145	37.0	35.0	37.0	17.0	37.0
7	35.09007078431636	37.0	35.0	37.0	32.0	37.0
8	35.62565417354554	37.0	35.0	37.0	35.0	37.0
9	37.486876168767644	39.0	37.0	39.0	35.0	39.0
10	37.427000670750104	39.0	37.0	39.0	35.0	39.0
11	37.31910119485771	39.0	37.0	39.0	34.0	39.0
12	37.22429768235658	39.0	37.0	39.0	34.0	39.0
13	37.20544941218075	39.0	37.0	39.0	34.0	39.0
14	38.42571875006006	40.0	38.0	41.0	34.0	41.0
15	38.533244412497865	40.0	38.0	41.0	34.0	41.0
16	38.607087464660694	40.0	38.0	41.0	35.0	41.0
17	38.59409048015329	40.0	38.0	41.0	35.0	41.0
18	38.55738661152037	40.0	38.0	41.0	35.0	41.0
19	38.54765785210056	40.0	38.0	41.0	34.0	41.0
20	38.477398219917625	40.0	38.0	41.0	34.0	41.0
21	38.44630924078391	40.0	38.0	41.0	34.0	41.0
22	38.42330481844582	40.0	37.0	41.0	34.0	41.0
23	38.359013709055894	40.0	37.0	41.0	34.0	41.0
24	38.35148362620192	40.0	37.0	41.0	34.0	41.0
25	38.290327168454375	40.0	37.0	41.0	34.0	41.0
26	38.19695933024929	40.0	37.0	41.0	34.0	41.0
27	38.158564171950346	40.0	37.0	41.0	34.0	41.0
28	38.074016985929624	40.0	37.0	41.0	34.0	41.0
29	38.059294117194845	40.0	37.0	41.0	34.0	41.0
30	37.944981194012065	40.0	37.0	41.0	34.0	41.0
31	33.350089273187486	39.0	34.0	41.0	9.0	41.0
32	34.23166920680437	39.0	33.0	41.0	16.0	41.0
33	36.19237555087034	39.0	34.0	41.0	30.0	41.0
34	37.08219571680892	39.0	35.0	41.0	32.0	41.0
35	37.23395725265369	39.0	35.0	41.0	32.0	41.0
36	37.40417402601896	39.0	36.0	41.0	33.0	41.0
37	37.487813104804225	40.0	36.0	41.0	33.0	41.0
38	37.419783860868364	40.0	36.0	41.0	33.0	41.0
39	37.47768266408873	40.0	36.0	41.0	33.0	41.0
40	37.45989048899413	40.0	36.0	41.0	33.0	41.0
41	37.4285218704895	40.0	36.0	41.0	33.0	41.0
42	37.360288903025676	40.0	36.0	41.0	33.0	41.0
43	37.34701612298751	40.0	35.0	41.0	33.0	41.0
44	37.271656675885474	40.0	35.0	41.0	32.0	41.0
45	37.232761818367024	39.0	35.0	41.0	32.0	41.0
46	37.18587081237639	39.0	35.0	41.0	32.0	41.0
47	37.094003993749915	39.0	35.0	41.0	32.0	41.0
48	37.09243762888877	39.0	35.0	41.0	32.0	41.0
49	37.05459406165135	39.0	35.0	41.0	32.0	41.0
50	36.87565369306552	39.0	35.0	41.0	32.0	41.0
51	36.26872382585098	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	8.0
11	9.0
12	10.0
13	9.0
14	25.0
15	29.0
16	42.0
17	100.0
18	172.0
19	340.0
20	593.0
21	958.0
22	1477.0
23	2358.0
24	3395.0
25	4538.0
26	5581.0
27	6791.0
28	8400.0
29	10711.0
30	14274.0
31	19598.0
32	30311.0
33	35765.0
34	63247.0
35	96387.0
36	118548.0
37	119882.0
38	180872.0
39	315841.0
40	350.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.633472544410765	22.224891555659767	24.288937620240127	10.85269827968934
2	32.56760834343943	24.281538227951252	24.57559199943111	18.575261429178205
3	32.70829289293176	24.500733212508624	24.914522604662963	17.876451289896657
4	28.844080390072897	26.17289977379001	25.33907474923748	19.643945086899617
5	22.459269708809888	39.57560160903149	20.34746393036499	17.617664751793633
6	26.725836179376643	34.118309555978804	22.981743681207274	16.174110583437276
7	79.88047579053378	6.569122816458554	8.377073031040931	5.173328361966739
8	79.69597146333072	6.36366956043766	8.170851006990024	5.769507969241591
9	73.92838541416417	8.293757795788306	10.514632538491254	7.263224251556275
10	36.592108980555935	33.383271223282904	17.033881529002734	12.990738267158422
11	26.87334354513533	25.835218416606924	28.490351000263303	18.80108703799444
12	26.746977300201998	23.421863378389546	30.757063536755762	19.0740957846527
13	23.10810992614061	25.347435101563864	31.663537140144488	19.88091783215103
14	18.697976025968984	30.09822933503487	29.994253458975656	21.209541180020487
15	17.573076206052896	29.027431565230927	33.7578534459066	19.64163878280958
16	20.058407151080214	28.156417387226533	31.367080968570843	20.41809449312241
17	19.614059229732874	27.41618987032805	28.639011518067008	24.330739381872064
18	19.965770603463685	28.330735538031917	30.43985062837177	21.26364323013263
19	21.6277509883474	27.83315043060619	28.92576199326175	21.61333658778466
20	23.282428076945994	28.341690482459597	29.321004856692028	19.054876583902384
21	22.97492086494091	27.83478406266997	29.094890959864543	20.095404112524577
22	20.653145318298794	26.138785692458192	29.500416095696245	23.70765289354677
23	20.48603436777478	27.826423710343583	29.211839796430226	22.47570212545141
24	21.543570889061005	26.41554218326277	29.42132908460869	22.61955784306754
25	20.63853872572855	29.29121509552904	26.898424602114495	23.171821576627917
26	20.55128355432211	30.88054690157655	27.44588353548729	21.122286008614047
27	23.409755281916848	28.07387092000392	28.341210002440835	20.175163795638397
28	19.191813389248395	28.91432656881531	30.184235258392544	21.709624783543752
29	21.385396866885895	28.276729583923522	27.78375708467788	22.55411646451271
30	23.274836492649616	28.407612341033182	27.248021863762773	21.069529302554425
31	25.550582053494725	29.762373801923076	24.43798252205884	20.249061622523364
32	23.118103910530778	30.861904276848744	25.489080611093705	20.530911201526774
33	22.231522179918624	30.80914757078912	25.385969599068254	21.573360650224
34	22.609756050684876	27.400430125712795	27.72590729041942	22.26390653318291
35	22.528843215526038	28.24636324673802	26.476370953637524	22.748422584098417
36	23.513923349983568	28.378495251896457	26.481367945832606	21.626213452287374
37	22.67769592533725	28.99725742005293	28.128645642142324	20.196401012467497
38	19.69401110485419	30.650204780583994	26.978280381232068	22.677503733329747
39	23.156734504038916	27.880621856459477	26.572082573374107	22.390561066127503
40	23.743784990957366	26.827025271327066	28.40665138099567	21.022538356719895
41	20.853601582124607	27.856309567510323	26.892851033896903	24.397237816468163
42	22.67000824503712	26.5780405256067	27.532466034867475	23.2194851944887
43	23.39783937745165	25.88451566653149	28.0304355263082	22.68720942970866
44	21.897876854893113	26.429380007802994	27.930687874414055	23.74205526288984
45	21.646681901086463	26.68855092992103	26.781571861552568	24.883195307439944
46	23.157118888053922	27.034688735434248	27.051217248079524	22.75697512843231
47	20.989769619440608	26.14061151652947	29.97762885032663	22.89199001370329
48	20.40310351653716	24.743663909992637	29.984547762596748	24.868684810873454
49	21.301985535629512	25.422101696478848	30.25717212524	23.018740642651633
50	20.67966781533423	24.3812858798454	29.628608164700864	25.31043814011951
51	20.020449229598338	25.00677476826449	27.05169772809828	27.921078274038898
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	990.0
1	1295.0
2	1600.0
3	1936.5
4	2273.0
5	1910.5
6	1548.0
7	1472.0
8	1396.0
9	1504.5
10	1613.0
11	1750.0
12	1887.0
13	2044.0
14	2201.0
15	2347.0
16	2493.0
17	2812.0
18	3131.0
19	3355.5
20	3580.0
21	3691.5
22	3803.0
23	5405.0
24	7007.0
25	7730.0
26	9892.0
27	11331.0
28	13316.0
29	15301.0
30	18651.0
31	22001.0
32	23532.0
33	25063.0
34	28606.5
35	32150.0
36	33998.0
37	35846.0
38	39009.5
39	42173.0
40	46752.0
41	51331.0
42	58180.0
43	65029.0
44	70815.0
45	76601.0
46	86221.0
47	95841.0
48	105565.5
49	115290.0
50	111186.5
51	107083.0
52	91344.5
53	75606.0
54	64328.5
55	53051.0
56	47224.0
57	41397.0
58	38826.0
59	36255.0
60	34116.0
61	31977.0
62	28987.5
63	25998.0
64	20646.5
65	15295.0
66	11875.5
67	8456.0
68	7153.5
69	5851.0
70	4671.0
71	3491.0
72	3160.0
73	2829.0
74	2260.5
75	1238.0
76	784.0
77	684.0
78	584.0
79	406.5
80	229.0
81	129.5
82	30.0
83	52.5
84	75.0
85	42.0
86	9.0
87	5.0
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1040626.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.731745599276792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.34379535780566	16.655296144790068
2	13.636536566297252	6.745107064257983
3	5.907063882517665	4.382760035433094
4	3.128355365833704	3.0947875620772662
5	1.7546447284734101	2.169771352085824
6	1.1186717421339318	1.6600022961333698
7	0.7657235596834626	1.3256376194224826
8	0.5011170008042217	0.9914798543490075
9	0.40875370353273416	0.9098273347680409
>10	3.4137839013215263	20.64165861409959
>50	1.8118162814669856	31.318751321300187
>100	0.19953923796669615	7.194065795168041
>500	0.007538148989852965	1.2292421264944904
>1k	0.002660523172889282	1.6816128796205296
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGCT	4597	0.44175332924605	TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	3781	0.3633389901847542	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	3387	0.3254771647066285	TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	1285	0.12348336482079056	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCC	1187	0.11406595645313494	No Hit
ACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	1097	0.10541731611549202	TruSeq Adapter, Index 15 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4701016503527684	0.0
2	0.0	0.0	0.0	1.4511457526527303	0.0
3	0.0	0.0	0.0	2.029835887244793	0.0
4	0.0	0.0	0.0	2.6907842010482153	0.0
5	0.0	0.0	0.0	4.33162346510658	0.0
6	0.0	0.0	0.0	5.34159246453577	0.0
7	0.0	0.0	0.0	6.20395800220252	0.0
8	0.0	0.0	0.0	7.619163849452157	0.0
9	0.0	0.0	0.0	8.13366185353816	0.0
10	0.0	0.0	0.0	9.235306440546363	0.0
11	0.0	0.0	0.0	10.991941389125392	0.0
12	0.0	0.0	0.0	12.419543620858983	0.0
13	0.0	0.0	0.0	12.989200731098396	0.0
14	0.0	0.0	0.0	13.246257541133895	0.0
15	0.0	0.0	0.0	13.710881719272823	0.0
16	0.0	0.0	0.0	14.621391354819119	0.0
17	0.0	0.0	0.0	15.693534468675585	0.0
18	0.0	0.0	0.0	16.814878736452865	0.0
19	0.0	0.0	0.0	17.525220396184604	0.0
20	0.0	0.0	0.0	18.21422874308349	0.0
21	0.0	0.0	0.0	18.93783165133295	0.0
22	0.0	0.0	0.0	19.72207113794966	0.0
23	0.0	0.0	0.0	20.40800441272849	0.0
24	0.0	0.0	0.0	20.984772627245523	0.0
25	0.0	0.0	0.0	21.500423783376544	0.0
26	0.0	0.0	0.0	21.953612537069034	0.0
27	3.8438401500635194E-4	0.0	0.0	22.4207352113055	0.0
28	3.8438401500635194E-4	0.0	0.0	22.90525126222101	0.0
29	3.8438401500635194E-4	0.0	0.0	23.43310661082848	0.0
30	3.8438401500635194E-4	0.0	0.0	24.008241193281737	0.0
31	3.8438401500635194E-4	0.0	0.0	24.53436681382168	0.0
32	3.8438401500635194E-4	0.0	0.0	25.097297203798483	0.0
33	3.8438401500635194E-4	0.0	0.0	25.626978376477236	0.0
34	3.8438401500635194E-4	0.0	0.0	26.15906194924978	0.0
35	4.8048001875793995E-4	0.0	0.0	26.72429864331662	0.0
36	4.8048001875793995E-4	0.0	0.0	27.310868650216314	0.0
37	4.8048001875793995E-4	0.0	0.0	27.858615871600364	0.0
38	5.76576022509528E-4	0.0	0.0	28.397233972628015	0.0
39	5.76576022509528E-4	0.0	0.0	28.966218410841165	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGTT	35	1.2117744E-7	45.000004	45
ATCTCCG	35	1.2117744E-7	45.000004	24
TTGGACG	30	2.1653032E-6	45.000004	1
CGAAACC	35	1.2117744E-7	45.000004	31
TTATGCG	30	2.1653032E-6	45.000004	1
CCGGAAC	30	2.1653032E-6	45.000004	28
ATAGACG	35	1.2117744E-7	45.000004	1
CCCGTCG	30	2.1653032E-6	45.000004	40
CGTCGCA	30	2.1653032E-6	45.000004	42
TAGGCCG	30	2.1653032E-6	45.000004	1
CGACTTA	35	1.2117744E-7	45.000004	29
TCGAACG	35	1.2117744E-7	45.000004	1
TATCCGA	30	2.1653032E-6	45.000004	30
ACGCGTT	30	2.1653032E-6	45.000004	26
ACGCGAT	40	6.8139343E-9	45.0	26
GTCGACG	20	7.0329796E-4	45.0	33
CTCCGAT	20	7.0329796E-4	45.0	11
TTTACGC	20	7.0329796E-4	45.0	26
CGCGGAC	25	3.8905506E-5	45.0	40
ACGTTCG	20	7.0329796E-4	45.0	41
>>END_MODULE