##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935130.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1680089
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.976034602928774	33.0	31.0	34.0	30.0	34.0
2	32.2892971741378	34.0	31.0	34.0	30.0	34.0
3	32.35737452004031	34.0	31.0	34.0	30.0	34.0
4	35.871575255834664	37.0	35.0	37.0	35.0	37.0
5	30.399121117988393	37.0	35.0	37.0	0.0	37.0
6	32.851814397927726	37.0	35.0	37.0	17.0	37.0
7	35.051538341123596	37.0	35.0	37.0	32.0	37.0
8	35.61808035169565	37.0	35.0	37.0	35.0	37.0
9	37.48719740442322	39.0	37.0	39.0	35.0	39.0
10	37.366477609221896	39.0	37.0	39.0	34.0	39.0
11	37.232358523863915	39.0	37.0	39.0	34.0	39.0
12	37.02981092073099	39.0	37.0	39.0	33.0	39.0
13	36.90946551045808	39.0	37.0	39.0	33.0	39.0
14	37.80657393745212	40.0	37.0	41.0	33.0	41.0
15	37.98091113030321	40.0	37.0	41.0	33.0	41.0
16	38.15856659974561	40.0	37.0	41.0	33.0	41.0
17	38.20051199668589	40.0	37.0	41.0	34.0	41.0
18	38.18743828451945	40.0	37.0	41.0	34.0	41.0
19	38.19471825599715	40.0	37.0	41.0	34.0	41.0
20	38.08790070049861	40.0	37.0	41.0	33.0	41.0
21	38.055003038529506	40.0	36.0	41.0	33.0	41.0
22	38.08939883541884	40.0	36.0	41.0	34.0	41.0
23	38.0368040026451	40.0	36.0	41.0	34.0	41.0
24	38.02349518388609	40.0	36.0	41.0	34.0	41.0
25	37.944949344945414	40.0	36.0	41.0	34.0	41.0
26	37.8821354106836	40.0	36.0	41.0	34.0	41.0
27	37.834100455392544	40.0	36.0	41.0	34.0	41.0
28	37.80567100909535	40.0	36.0	41.0	33.0	41.0
29	37.79726431159302	40.0	36.0	41.0	33.0	41.0
30	37.63380749472201	40.0	36.0	41.0	33.0	41.0
31	33.127844417765964	38.0	33.0	41.0	9.0	41.0
32	34.05444473477298	38.0	33.0	41.0	16.0	41.0
33	35.95870992548609	38.0	34.0	41.0	29.0	41.0
34	36.795467382977925	38.0	35.0	41.0	31.0	41.0
35	36.97248776701711	39.0	35.0	41.0	31.0	41.0
36	37.13765818358432	39.0	35.0	41.0	32.0	41.0
37	37.25000342243774	39.0	35.0	41.0	32.0	41.0
38	37.2635181826677	39.0	35.0	41.0	32.0	41.0
39	37.24801067086327	39.0	35.0	41.0	32.0	41.0
40	37.19593783424568	39.0	35.0	41.0	32.0	41.0
41	37.12468982297962	39.0	35.0	41.0	32.0	41.0
42	37.11088579235981	39.0	35.0	41.0	32.0	41.0
43	37.13993961034207	39.0	35.0	41.0	32.0	41.0
44	37.0851413228704	39.0	35.0	41.0	32.0	41.0
45	37.06525428117201	39.0	35.0	41.0	32.0	41.0
46	37.036257007813276	39.0	35.0	41.0	32.0	41.0
47	36.94938422904977	39.0	35.0	41.0	32.0	41.0
48	36.94260065984599	39.0	35.0	41.0	32.0	41.0
49	36.930246552414786	39.0	35.0	41.0	32.0	41.0
50	36.75049000380337	39.0	35.0	41.0	32.0	41.0
51	36.146915431265846	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	8.0
11	16.0
12	16.0
13	26.0
14	39.0
15	55.0
16	124.0
17	195.0
18	322.0
19	589.0
20	969.0
21	1529.0
22	2530.0
23	3838.0
24	5618.0
25	7547.0
26	9545.0
27	11804.0
28	15324.0
29	19947.0
30	27044.0
31	38881.0
32	55740.0
33	69126.0
34	126325.0
35	150765.0
36	190131.0
37	193048.0
38	290369.0
39	458180.0
40	431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.41305728446529	21.402080485021923	24.077474467126443	11.107387763386345
2	32.02098222177516	23.290135224979153	25.144620314757134	19.544262238488557
3	33.057653493356604	23.087348348807712	25.74976682782876	18.105231330006923
4	28.985845392714317	25.221580523412747	26.586389173430693	19.206184910442246
5	23.051457393030965	38.27309148503442	20.95216384370114	17.723287278233474
6	27.495805281744	31.797244074569857	24.605839333511497	16.10111131017464
7	79.3458560826242	5.597798688045693	9.172728349510056	5.883616879820057
8	79.69809932688091	4.952594773253083	9.040890095703263	6.308415804162755
9	73.89578766362972	6.961119321654984	11.257558379347762	7.885534635367532
10	40.50446137079643	27.07791075353746	18.291114339776048	14.126513535890062
11	29.53998270329727	23.76856226068976	26.512524038905084	20.17893099710789
12	27.850786476192628	21.2660757852709	30.59034372583833	20.29279401269814
13	25.663283314157763	22.949855632648035	32.46185172333133	18.92500932986288
14	20.13423098419191	26.043382225584477	32.56238211190002	21.26000467832359
15	18.857036740315543	25.979635602637718	35.074629974959656	20.08869768208708
16	24.149673023274364	26.224860706783986	29.31773257250062	20.307733697441027
17	23.780823515897072	25.92047207022961	27.174512778787314	23.124191635085996
18	23.669996053780483	24.632147463616512	30.537846506941	21.160009975662003
19	23.338465997932254	26.693764437479206	27.73091187431142	22.236857690277123
20	22.410122320900857	29.968829032271504	28.63199509073626	18.989053556091374
21	21.97324070331988	27.027794360893974	31.909202429157023	19.089762506629114
22	20.885679270562452	22.656299755548666	32.15793925202772	24.300081721861165
23	20.096375846755738	25.56322909083983	31.793137149281975	22.547257913122458
24	22.159183233745356	26.70834699828402	28.353378898379788	22.77909086959084
25	22.32667436070351	27.440212988716667	26.131948962227597	24.101163688352223
26	19.705503696530364	27.616156048875983	26.688467099064393	25.98987315552926
27	20.67420237856447	26.686145793466892	29.022926761617985	23.616725066350654
28	17.691800851026347	26.624720476117638	28.376115789104027	27.30736288375199
29	19.659851353112842	26.515797675004123	27.82971616384608	25.994634808036952
30	21.898839882887156	27.582645919353084	27.651213715463886	22.867300482295878
31	23.28114760587088	27.75448205422451	25.127180762447704	23.837189577456908
32	21.821820153575196	27.11171848634209	26.126651623812787	24.939809736269925
33	21.236077374472423	27.62835778342695	25.68262752747027	25.452937314630358
34	18.96465008698944	26.373364744367713	28.606579770476444	26.05540539816641
35	18.888642208835364	27.463902209942447	26.89869405727911	26.748761523943077
36	20.861811487367635	28.56021317918277	24.609232010923233	25.968743322526365
37	18.48919908409614	28.774606583341715	27.75025608762393	24.985938244938215
38	19.808057787414835	26.5791871740128	27.13838374038518	26.474371298187176
39	19.983822285605108	25.74500517532107	27.93643670067479	26.334735838399038
40	21.864853588113487	25.761790000410695	27.845072493183398	24.528283918292423
41	18.60115743868331	25.71756615274548	25.891306948619984	29.789969459951227
42	19.506109497770655	24.26758344349615	28.40837598484366	27.81793107388954
43	20.33808923217758	24.226990355868054	28.784903656889604	26.650016755064758
44	20.534566918776324	24.467275245537586	27.91155706632208	27.086600769364004
45	19.974834666496836	23.441377212754798	27.004283701637235	29.57950441911113
46	21.10810796332813	25.2028315166637	27.30849377622257	26.3805667437856
47	17.230218161061707	25.401987632798022	30.8044990473719	26.563295158768373
48	17.834233781662757	26.12736587168894	28.78668927657999	27.251711070068314
49	20.751281628532777	23.591547828716216	30.06459776833251	25.5925727744185
50	19.334630486837305	23.743265981742635	29.352373594494104	27.569729936925963
51	18.471819052443056	23.908673885728675	27.209332362749834	30.41017469907844
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	936.0
1	1432.0
2	1928.0
3	2403.0
4	2878.0
5	2369.0
6	1860.0
7	1867.0
8	1874.0
9	2018.5
10	2163.0
11	2189.0
12	2215.0
13	2413.5
14	2612.0
15	2812.5
16	3013.0
17	3366.5
18	3720.0
19	3817.5
20	3915.0
21	4525.0
22	5135.0
23	5967.0
24	6799.0
25	8209.5
26	12410.5
27	15201.0
28	17730.0
29	20259.0
30	23556.5
31	26854.0
32	28482.5
33	30111.0
34	36373.5
35	42636.0
36	45869.0
37	49102.0
38	53921.5
39	58741.0
40	64817.0
41	70893.0
42	79043.0
43	87193.0
44	100958.5
45	114724.0
46	134207.0
47	153690.0
48	172215.0
49	190740.0
50	190239.5
51	189739.0
52	163640.0
53	137541.0
54	122723.5
55	107906.0
56	96023.5
57	84141.0
58	78389.5
59	72638.0
60	63286.5
61	53935.0
62	47036.5
63	40138.0
64	34219.5
65	28301.0
66	23773.0
67	19245.0
68	16870.0
69	14495.0
70	11822.5
71	9150.0
72	7168.0
73	5186.0
74	4870.0
75	3498.0
76	2442.0
77	1646.5
78	851.0
79	758.0
80	665.0
81	450.0
82	235.0
83	148.5
84	62.0
85	45.5
86	29.0
87	24.0
88	19.0
89	11.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1680089.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.85774827075081
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.69906277675283	16.579885123995272
2	13.731123697872771	6.8264963271252475
3	5.927356910798581	4.420222379985789
4	3.155794616922175	3.1378379272656765
5	1.795804547435266	2.2319828691807717
6	1.1385963444126492	1.6981764786844034
7	0.8166815707614554	1.4210605432344738
8	0.5640358310326535	1.1216528562794752
9	0.4425162834596483	0.9899962541953333
>10	3.883191498069274	22.15060960090008
>50	1.6565172749079968	28.773042207867128
>100	0.17848496251901988	6.713001107251891
>500	0.005687684654284497	0.9455831922936725
>1k	0.004604316148706497	2.1320561433646152
>5k	5.416842527889998E-4	0.8583969883762425
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	7160	0.4261678994386607	Illumina PCR Primer Index 8 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT	5590	0.3327204689751555	RNA PCR Primer, Index 29 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	4853	0.2888537452480196	RNA PCR Primer, Index 29 (96% over 25bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	4087	0.24326092248684447	No Hit
TCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	2226	0.13249298102660037	Illumina PCR Primer Index 8 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2001	0.11910083334870951	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC	1802	0.10725622273581935	No Hit
ACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	1707	0.10160176038293209	TruSeq Adapter, Index 12 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCG	1704	0.10142319841389355	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1904131269236332E-4	0.0	0.0	0.35146947572420273	0.0
2	1.1904131269236332E-4	0.0	0.0	1.3561186341914029	0.0
3	1.7856196903854498E-4	0.0	0.0	1.9523965694674508	0.0
4	1.7856196903854498E-4	0.0	0.0	2.696940459701837	0.0
5	1.7856196903854498E-4	0.0	0.0	4.519165353740188	0.0
6	1.7856196903854498E-4	0.0	0.0	5.63291587528994	0.0
7	1.7856196903854498E-4	0.0	0.0	6.638160240320602	0.0
8	1.7856196903854498E-4	0.0	0.0	8.227837930014422	0.0
9	1.7856196903854498E-4	0.0	0.0	8.79590307418238	0.0
10	1.7856196903854498E-4	0.0	0.0	10.055717286405661	0.0
11	1.7856196903854498E-4	0.0	0.0	11.897822079663637	0.0
12	1.7856196903854498E-4	0.0	0.0	13.442025987908973	0.0
13	1.7856196903854498E-4	0.0	0.0	14.060981293252917	0.0
14	1.7856196903854498E-4	0.0	0.0	14.346323319776511	0.0
15	1.7856196903854498E-4	0.0	0.0	14.859093774198866	0.0
16	1.7856196903854498E-4	0.0	0.0	15.752974991205823	0.0
17	1.7856196903854498E-4	0.0	0.0	16.78119432958611	0.0
18	2.3808262538472665E-4	0.0	0.0	17.832567203285063	0.0
19	2.3808262538472665E-4	0.0	0.0	18.624787139252742	0.0
20	2.3808262538472665E-4	0.0	0.0	19.299453778936712	0.0
21	2.3808262538472665E-4	0.0	0.0	20.04953309021129	0.0
22	2.3808262538472665E-4	0.0	0.0	20.865323206092057	0.0
23	2.976032817309083E-4	0.0	0.0	21.60123660115625	0.0
24	2.976032817309083E-4	0.0	0.0	22.195371792803833	0.0
25	2.976032817309083E-4	0.0	0.0	22.747902045665437	0.0
26	2.976032817309083E-4	0.0	0.0	23.249244534069327	0.0
27	2.976032817309083E-4	0.0	0.0	23.84885562610076	1.1904131269236332E-4
28	2.976032817309083E-4	0.0	0.0	24.380494128584854	1.1904131269236332E-4
29	2.976032817309083E-4	0.0	0.0	25.020341184306307	1.1904131269236332E-4
30	2.976032817309083E-4	0.0	0.0	25.70774524444836	1.1904131269236332E-4
31	3.5712393807708995E-4	0.0	0.0	26.243550192876686	1.1904131269236332E-4
32	3.5712393807708995E-4	0.0	0.0	26.795961404425597	1.1904131269236332E-4
33	3.5712393807708995E-4	0.0	0.0	27.380751853026833	1.1904131269236332E-4
34	3.5712393807708995E-4	0.0	0.0	27.99512406783212	1.1904131269236332E-4
35	3.5712393807708995E-4	0.0	0.0	28.645446759070502	1.1904131269236332E-4
36	3.5712393807708995E-4	0.0	0.0	29.23595119068097	1.1904131269236332E-4
37	3.5712393807708995E-4	0.0	0.0	29.847287852012602	1.1904131269236332E-4
38	3.5712393807708995E-4	0.0	0.0	30.5386202754735	1.1904131269236332E-4
39	3.5712393807708995E-4	0.0	0.0	31.632848021741705	1.1904131269236332E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	30	2.1661035E-6	45.000004	1
CTACCGA	20	7.0342165E-4	45.000004	41
ATACGAT	20	7.0342165E-4	45.000004	42
TTCGATC	20	7.0342165E-4	45.000004	20
AATCCGT	25	3.891578E-5	45.0	9
CGGCATA	25	3.891578E-5	45.0	33
CGCATCG	25	3.891578E-5	45.0	21
ATAGCGT	25	3.891578E-5	45.0	40
TACGCTA	25	3.891578E-5	45.0	1
TAATACG	90	0.0	42.5	4
GCGTAAG	180	0.0	42.5	1
TTGCACG	135	0.0	41.666664	1
TACGTCA	50	1.0822987E-9	40.5	27
CGCCAAT	260	0.0	39.80769	26
TGTTACG	80	0.0	39.375004	1
TGCGTAG	190	0.0	39.078945	1
CTATCCG	35	6.250011E-6	38.57143	33
ATCCGAT	35	6.250011E-6	38.57143	35
GTAGCGT	70	0.0	38.57143	9
GTATCGC	35	6.250011E-6	38.57143	29
>>END_MODULE