SRR2935129.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935129.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	639319
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT	4124	0.6450613856306476	TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	3799	0.5942260436495709	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	3176	0.4967786034827684	TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	1300	0.203341367924307	TruSeq Adapter, Index 16 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	1195	0.18691764205349756	TruSeq Adapter, Index 13 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1070	0.16736558744539112	No Hit
TGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCTT	1007	0.15751135192290547	TruSeq Adapter, Index 16 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCC	853	0.13342322064571832	No Hit
ACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG	759	0.11872007558042229	No Hit
GCCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG	744	0.11637382902744953	No Hit
GGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG	729	0.11402758247447675	No Hit
AAAACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC	717	0.11215058523209853	No Hit
AGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG	648	0.10135785108842377	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCGT	25	3.8888556E-5	45.000004	29
TCGTTGA	25	3.8888556E-5	45.000004	24
TCCGCTC	25	3.8888556E-5	45.000004	40
GTCGATG	35	1.2107921E-7	45.000004	1
GATAACG	25	3.8888556E-5	45.000004	12
GGTCGCT	25	3.8888556E-5	45.000004	8
CAACGAC	25	3.8888556E-5	45.000004	12
GCAACGA	25	3.8888556E-5	45.000004	11
TTATGCG	25	3.8888556E-5	45.000004	14
CGTTGAT	25	3.8888556E-5	45.000004	25
CACGCGA	35	1.2107921E-7	45.000004	13
CGACCGA	35	1.2107921E-7	45.000004	17
GCACGAA	25	3.8888556E-5	45.000004	1
AATGCGG	50	2.1827873E-11	45.000004	2
GTTCGCA	25	3.8888556E-5	45.000004	39
TCTCGCG	25	3.8888556E-5	45.000004	1
ACGTGAC	25	3.8888556E-5	45.000004	14
CGGGCAC	50	2.1827873E-11	45.000004	6
CGAATAT	35	1.2107921E-7	45.000004	14
TCCGTGT	25	3.8888556E-5	45.000004	13