##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935129.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	639319
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92924971727729	33.0	31.0	34.0	30.0	34.0
2	32.249305902061415	34.0	31.0	34.0	30.0	34.0
3	32.308686273988414	34.0	31.0	34.0	30.0	34.0
4	35.83808396121498	37.0	35.0	37.0	35.0	37.0
5	30.342702156513415	37.0	35.0	37.0	0.0	37.0
6	32.81815181466529	37.0	35.0	37.0	17.0	37.0
7	35.01956143959432	37.0	35.0	37.0	32.0	37.0
8	35.590833371133975	37.0	35.0	37.0	35.0	37.0
9	37.433326711704176	39.0	37.0	39.0	35.0	39.0
10	37.315283919295375	39.0	37.0	39.0	34.0	39.0
11	37.246313655624185	39.0	37.0	39.0	34.0	39.0
12	37.088534831594245	39.0	37.0	39.0	34.0	39.0
13	37.0029437573418	39.0	37.0	39.0	33.0	39.0
14	38.144583533416025	40.0	37.0	41.0	33.0	41.0
15	38.27251810129216	40.0	37.0	41.0	34.0	41.0
16	38.36686067518719	40.0	37.0	41.0	34.0	41.0
17	38.35325244517995	40.0	37.0	41.0	34.0	41.0
18	38.28311687905412	40.0	37.0	41.0	34.0	41.0
19	38.26557008316662	40.0	37.0	41.0	34.0	41.0
20	38.17698676247695	40.0	37.0	41.0	34.0	41.0
21	38.12940644654703	40.0	37.0	41.0	34.0	41.0
22	38.10582823285402	40.0	36.0	41.0	34.0	41.0
23	38.031882362326165	40.0	36.0	41.0	34.0	41.0
24	38.0010526826201	40.0	36.0	41.0	34.0	41.0
25	37.93296773598157	40.0	36.0	41.0	34.0	41.0
26	37.83903184482238	40.0	36.0	41.0	33.0	41.0
27	37.81012139479665	40.0	36.0	41.0	33.0	41.0
28	37.767616792243	40.0	36.0	41.0	33.0	41.0
29	37.76361878811673	40.0	36.0	41.0	33.0	41.0
30	37.65863363985741	40.0	36.0	41.0	33.0	41.0
31	33.09117983354163	38.0	33.0	41.0	9.0	41.0
32	33.95884370713212	38.0	33.0	41.0	16.0	41.0
33	35.92278189761293	38.0	34.0	41.0	29.0	41.0
34	36.78514638232244	38.0	35.0	41.0	31.0	41.0
35	36.92908235168984	39.0	35.0	41.0	32.0	41.0
36	37.05217426668064	39.0	35.0	41.0	32.0	41.0
37	37.192124745236725	39.0	35.0	41.0	33.0	41.0
38	37.1671356552832	39.0	35.0	41.0	33.0	41.0
39	37.182049962538265	39.0	35.0	41.0	33.0	41.0
40	37.174227576530654	39.0	35.0	41.0	33.0	41.0
41	37.11112605757064	39.0	35.0	41.0	32.0	41.0
42	36.98381559127759	39.0	35.0	41.0	32.0	41.0
43	36.97559590752034	39.0	35.0	41.0	32.0	41.0
44	36.887162746610066	39.0	35.0	41.0	32.0	41.0
45	36.89301897800628	39.0	35.0	41.0	32.0	41.0
46	36.82176816268561	39.0	35.0	41.0	32.0	41.0
47	36.738853373667915	39.0	35.0	41.0	31.0	41.0
48	36.75987887111129	39.0	35.0	41.0	32.0	41.0
49	36.75058617059715	39.0	35.0	41.0	32.0	41.0
50	36.56316173928821	39.0	35.0	41.0	31.0	41.0
51	35.962289561236254	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	5.0
12	7.0
13	9.0
14	16.0
15	24.0
16	32.0
17	86.0
18	152.0
19	283.0
20	479.0
21	869.0
22	1302.0
23	2061.0
24	2983.0
25	3482.0
26	4195.0
27	4756.0
28	5834.0
29	7242.0
30	9581.0
31	13425.0
32	20939.0
33	24030.0
34	41992.0
35	64441.0
36	72649.0
37	71656.0
38	111107.0
39	175513.0
40	164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.46205728282751	22.128233323270543	23.906375377550173	11.503334016351774
2	31.11748594989356	25.054941273448776	24.51718156350742	19.310391213150243
3	30.993134882586006	25.736134855995207	26.198189010493977	17.07254125092481
4	26.329265984586726	27.358016889846855	25.814186657990767	20.498530467575655
5	21.36632885930185	39.72821079930363	21.330978744570395	17.57448159682412
6	26.1963120132516	33.06330642449231	24.497629508899312	16.242752053356774
7	76.82143030318198	6.905160021835735	9.713147896433549	6.560261778548737
8	76.4737165640314	5.740483232940051	10.107786566643568	7.678013636384966
9	70.96676307133059	8.543622197995054	12.503304297228771	7.986310433445588
10	36.143771732108696	29.504676069380075	20.035381398018828	14.316170800492397
11	26.222120725334303	27.319225613504372	26.632088206357075	19.82656545480425
12	24.462122977730992	24.042457677622593	31.2200951324769	20.27532421216951
13	21.91894813074537	27.935506374751885	31.36994207899343	18.775603415509316
14	16.937866698784177	32.394156907584474	29.222500817275883	21.445475576355467
15	15.87532984316124	29.735233897318864	34.7246054004339	19.664830859085995
16	18.210470829116606	29.3643705255123	31.347887361395482	21.077271283975605
17	18.541291593085766	28.265232223662988	26.769734670798144	26.4237415124531
18	18.825969508179796	27.505830422684134	31.740961867236855	21.927238201899208
19	19.92479497715538	28.85820693581764	28.406945515462546	22.81005257156443
20	21.278735654657535	30.21308611194099	28.071275841950577	20.436902391450904
21	20.753645676102227	29.911828054539285	29.506709483059318	19.82781678629917
22	19.50184493187282	27.496132603598518	27.783000348808656	25.21902211572001
23	18.403957961518426	28.35282542830731	28.57227768922869	24.670938920945567
24	20.464744517212846	27.246179137488486	29.310250438357066	22.978825906941605
25	17.84367428466853	30.328521442347245	27.1642169245713	24.66358734841292
26	17.47421866079375	32.05989498200429	26.61425673255448	23.851629624647476
27	19.389694346640724	29.704419859256493	26.66196374579826	24.243922048304526
28	16.038785019685008	29.17886063139059	28.709767737232898	26.072586611691502
29	16.437803350127243	28.484684484584378	27.567614915245752	27.509897250042627
30	18.441967155676586	28.428218150876166	26.927715272031644	26.202099421415596
31	21.955549576971748	28.641883003633556	22.741855005091356	26.660712414303344
32	18.107392397222668	29.73179273570784	25.569082101423547	26.591732765645947
33	18.651095931764893	27.76798437086963	26.654925006139347	26.925994691226133
34	16.82555969711521	25.941666054035622	27.814909301929085	29.41786494692008
35	16.644898712536307	26.439852405450175	28.71758855907614	28.197660322937374
36	18.533940020553118	29.15664949735578	27.60875243814121	24.70065804394989
37	17.194702488116263	27.07943921579055	29.486844595577484	26.239013700515706
38	17.092249721969782	27.876224545180108	27.76485604213233	27.266669690717784
39	19.700806639564913	26.70998359191577	29.12083013331373	24.468379635205586
40	20.01207534892597	24.879129198412684	30.268770363464874	24.840025089196473
41	16.878741285649262	26.906755469491756	28.227848695252295	27.986654549606694
42	19.385314686408506	25.670909201822568	28.579472845324478	26.36430326644445
43	21.351469297799692	24.860046393115173	27.83962309895373	25.94886121013141
44	18.637956951068247	25.200095726859363	26.79632546506517	29.365621857007223
45	19.0729510619894	23.56382338081615	27.22537575138546	30.13784980580899
46	21.253709024759157	24.51170698821715	29.0310470985533	25.20353688847039
47	17.10851703140373	24.7860614184781	31.93335408458062	26.172067465537545
48	17.30919931990133	22.986177479474254	31.584858263245735	28.119764937378676
49	19.991741212133533	21.64944261002723	32.91330306153892	25.44551311630031
50	18.792339974253856	21.493182589599247	32.1679787398779	27.546498696269
51	17.388658869828678	21.773637260897925	29.16587806713081	31.671825802142596
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1254.0
1	1313.0
2	1372.0
3	1455.0
4	1538.0
5	1371.0
6	1204.0
7	1212.5
8	1221.0
9	1401.0
10	1581.0
11	1656.0
12	1731.0
13	1803.0
14	1875.0
15	2108.0
16	2341.0
17	2369.0
18	2397.0
19	2446.0
20	2495.0
21	2474.5
22	2454.0
23	2700.5
24	2947.0
25	3112.0
26	4278.0
27	5279.0
28	6526.0
29	7773.0
30	9200.5
31	10628.0
32	11009.5
33	11391.0
34	13007.5
35	14624.0
36	17200.5
37	19777.0
38	21010.5
39	22244.0
40	25457.0
41	28670.0
42	33662.5
43	38655.0
44	45287.5
45	51920.0
46	60333.0
47	68746.0
48	77896.5
49	87047.0
50	82378.5
51	77710.0
52	62847.0
53	47984.0
54	41252.5
55	34521.0
56	29938.0
57	25355.0
58	21854.5
59	18354.0
60	15632.0
61	12910.0
62	11407.0
63	9904.0
64	8224.5
65	6545.0
66	5452.0
67	4359.0
68	3403.0
69	2447.0
70	2296.5
71	2146.0
72	1600.0
73	1054.0
74	916.0
75	583.5
76	389.0
77	344.5
78	300.0
79	186.0
80	72.0
81	50.5
82	29.0
83	16.0
84	3.0
85	2.0
86	1.0
87	8.5
88	16.0
89	8.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	639319.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.99126193933229
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.2163305599656	19.094611772440754
2	13.444095244111317	7.526343830305028
3	5.841910161952454	4.905673127077748
4	3.0771103029910973	3.445288020289678
5	1.7973729451429732	2.5155368455083043
6	1.0799726337022586	1.8137878126362288
7	0.7626135056382042	1.4942560076354008
8	0.5035995426710007	1.1277109369145544
9	0.3784993453470597	0.9535206887527807
>10	3.532962410269707	24.720174221437517
>50	1.2604430295287083	23.648935226558663
>100	0.09547839928814314	4.940112205889241
>500	0.005126357008759364	1.0031968472710233
>1k	0.004485562382664443	2.8108524572830693
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT	4124	0.6450613856306476	TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	3799	0.5942260436495709	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	3176	0.4967786034827684	TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	1300	0.203341367924307	TruSeq Adapter, Index 16 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	1195	0.18691764205349756	TruSeq Adapter, Index 13 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1070	0.16736558744539112	No Hit
TGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCTT	1007	0.15751135192290547	TruSeq Adapter, Index 16 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCC	853	0.13342322064571832	No Hit
ACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG	759	0.11872007558042229	No Hit
GCCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG	744	0.11637382902744953	No Hit
GGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG	729	0.11402758247447675	No Hit
AAAACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC	717	0.11215058523209853	No Hit
AGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG	648	0.10135785108842377	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5641643686485152E-4	0.0	0.0	0.6768139223142124	0.0
2	1.5641643686485152E-4	0.0	0.0	2.24363737038943	0.0
3	1.5641643686485152E-4	0.0	0.0	3.1311442331605974	0.0
4	1.5641643686485152E-4	0.0	0.0	4.201501910626776	0.0
5	1.5641643686485152E-4	0.0	0.0	6.65129614480408	0.0
6	1.5641643686485152E-4	0.0	0.0	8.148670694911305	0.0
7	1.5641643686485152E-4	0.0	0.0	9.425498069039087	0.0
8	1.5641643686485152E-4	0.0	0.0	11.517255079232745	0.0
9	1.5641643686485152E-4	0.0	0.0	12.26602056250479	0.0
10	1.5641643686485152E-4	0.0	0.0	13.731173326617855	0.0
11	1.5641643686485152E-4	0.0	0.0	15.997960329663282	0.0
12	1.5641643686485152E-4	0.0	0.0	17.74247285001697	0.0
13	1.5641643686485152E-4	0.0	0.0	18.45401122131518	0.0
14	1.5641643686485152E-4	0.0	0.0	18.761369519754613	0.0
15	3.1283287372970304E-4	0.0	0.0	19.309452714529055	0.0
16	3.1283287372970304E-4	0.0	0.0	20.442064133867444	0.0
17	3.1283287372970304E-4	0.0	0.0	21.714042598452416	0.0
18	3.1283287372970304E-4	0.0	0.0	23.090663659299974	0.0
19	3.1283287372970304E-4	0.0	0.0	23.987242675409302	0.0
20	3.1283287372970304E-4	0.0	0.0	24.77870984594545	0.0
21	3.1283287372970304E-4	0.0	0.0	25.6307101775483	0.0
22	3.1283287372970304E-4	0.0	0.0	26.474576854434172	0.0
23	3.1283287372970304E-4	0.0	0.0	27.272300682444914	0.0
24	3.1283287372970304E-4	0.0	0.0	27.91955189819167	0.0
25	3.1283287372970304E-4	0.0	0.0	28.52441425954805	0.0
26	3.1283287372970304E-4	0.0	0.0	29.079379777544542	0.0
27	3.1283287372970304E-4	0.0	0.0	29.595084769887958	0.0
28	3.1283287372970304E-4	0.0	0.0	30.15130161937937	0.0
29	3.1283287372970304E-4	0.0	0.0	30.718780452325053	0.0
30	3.1283287372970304E-4	0.0	0.0	31.37776290083667	0.0
31	3.1283287372970304E-4	0.0	0.0	31.95228047344127	0.0
32	3.1283287372970304E-4	0.0	0.0	32.57403580997906	0.0
33	6.256657474594061E-4	0.0	0.0	33.18703182605241	0.0
34	6.256657474594061E-4	0.0	0.0	33.755761990493006	0.0
35	6.256657474594061E-4	0.0	0.0	34.38768439542701	0.0
36	7.820821843242575E-4	0.0	0.0	34.95438114618837	0.0
37	7.820821843242575E-4	0.0	0.0	35.497615431419995	0.0
38	7.820821843242575E-4	0.0	0.0	36.080110242304706	0.0
39	7.820821843242575E-4	0.0	0.0	36.663230718936866	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCGT	25	3.8888556E-5	45.000004	29
TCGTTGA	25	3.8888556E-5	45.000004	24
TCCGCTC	25	3.8888556E-5	45.000004	40
GTCGATG	35	1.2107921E-7	45.000004	1
GATAACG	25	3.8888556E-5	45.000004	12
GGTCGCT	25	3.8888556E-5	45.000004	8
CAACGAC	25	3.8888556E-5	45.000004	12
GCAACGA	25	3.8888556E-5	45.000004	11
TTATGCG	25	3.8888556E-5	45.000004	14
CGTTGAT	25	3.8888556E-5	45.000004	25
CACGCGA	35	1.2107921E-7	45.000004	13
CGACCGA	35	1.2107921E-7	45.000004	17
GCACGAA	25	3.8888556E-5	45.000004	1
AATGCGG	50	2.1827873E-11	45.000004	2
GTTCGCA	25	3.8888556E-5	45.000004	39
TCTCGCG	25	3.8888556E-5	45.000004	1
ACGTGAC	25	3.8888556E-5	45.000004	14
CGGGCAC	50	2.1827873E-11	45.000004	6
CGAATAT	35	1.2107921E-7	45.000004	14
TCCGTGT	25	3.8888556E-5	45.000004	13
>>END_MODULE