##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935126.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	395073
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.027336720049206	33.0	31.0	34.0	30.0	34.0
2	32.33417368435707	34.0	31.0	34.0	30.0	34.0
3	32.38491620535952	34.0	31.0	34.0	30.0	34.0
4	35.909454708370355	37.0	35.0	37.0	35.0	37.0
5	30.39671149382519	37.0	35.0	37.0	0.0	37.0
6	32.87692907386736	37.0	35.0	37.0	17.0	37.0
7	35.04392352805684	37.0	35.0	37.0	32.0	37.0
8	35.59789962867622	37.0	35.0	37.0	35.0	37.0
9	37.44705408873803	39.0	37.0	39.0	35.0	39.0
10	37.3887585332331	39.0	37.0	39.0	35.0	39.0
11	37.25620834630562	39.0	37.0	39.0	34.0	39.0
12	36.9736656263526	39.0	35.0	39.0	33.0	39.0
13	36.83253221556522	39.0	35.0	39.0	33.0	39.0
14	37.98247918739069	40.0	36.0	41.0	33.0	41.0
15	38.102181115895036	40.0	37.0	41.0	33.0	41.0
16	38.18891192260671	40.0	36.0	41.0	34.0	41.0
17	38.194799442128414	40.0	36.0	41.0	34.0	41.0
18	38.14920027437967	40.0	36.0	41.0	34.0	41.0
19	38.102221614739555	40.0	36.0	41.0	34.0	41.0
20	38.04814806377556	40.0	36.0	41.0	34.0	41.0
21	37.98863501175732	40.0	36.0	41.0	34.0	41.0
22	37.96981317376789	40.0	36.0	41.0	34.0	41.0
23	37.92062226474601	40.0	35.0	41.0	34.0	41.0
24	37.90069177088791	40.0	35.0	41.0	34.0	41.0
25	37.82010413265397	40.0	35.0	41.0	34.0	41.0
26	37.73803069306179	40.0	35.0	41.0	33.0	41.0
27	37.7011058715731	40.0	35.0	41.0	33.0	41.0
28	37.61270195634731	39.0	35.0	41.0	33.0	41.0
29	37.61485598864007	39.0	35.0	41.0	33.0	41.0
30	37.496558863804914	39.0	35.0	41.0	33.0	41.0
31	32.925925082199996	38.0	33.0	41.0	9.0	41.0
32	33.80315789740124	38.0	33.0	41.0	16.0	41.0
33	35.74588493772037	38.0	34.0	41.0	29.0	41.0
34	36.53166123729032	38.0	35.0	40.0	31.0	41.0
35	36.69102925282163	39.0	35.0	40.0	31.0	41.0
36	36.83384083447869	39.0	35.0	41.0	32.0	41.0
37	36.87999686133955	39.0	35.0	41.0	32.0	41.0
38	36.88330511070106	39.0	35.0	41.0	32.0	41.0
39	36.88197624236533	39.0	35.0	41.0	32.0	41.0
40	36.82682440966606	39.0	35.0	41.0	32.0	41.0
41	36.794453177007796	39.0	35.0	41.0	32.0	41.0
42	36.69571446289673	39.0	35.0	41.0	32.0	41.0
43	36.64926988176869	39.0	35.0	41.0	31.0	41.0
44	36.58141659895766	39.0	35.0	41.0	31.0	41.0
45	36.5640451258375	39.0	35.0	41.0	31.0	41.0
46	36.557899426182	39.0	35.0	41.0	31.0	41.0
47	36.46982962642347	39.0	35.0	41.0	31.0	41.0
48	36.460180776717216	39.0	35.0	41.0	31.0	41.0
49	36.40689442204352	39.0	35.0	40.0	31.0	41.0
50	36.207842601240785	38.0	35.0	40.0	31.0	41.0
51	35.610856727743986	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	4.0
12	6.0
13	8.0
14	9.0
15	12.0
16	29.0
17	71.0
18	114.0
19	179.0
20	332.0
21	506.0
22	806.0
23	1220.0
24	1788.0
25	2225.0
26	2564.0
27	3138.0
28	4017.0
29	4695.0
30	6148.0
31	8863.0
32	14582.0
33	15758.0
34	30043.0
35	44165.0
36	43489.0
37	43399.0
38	65483.0
39	101287.0
40	127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.929028306161136	22.25335570894493	24.01632103434049	10.80129495055344
2	32.492729191820246	25.455042485819074	23.87432196075156	18.177906361609118
3	32.05255737547238	24.55773996198171	24.975384296066803	18.414318366479108
4	28.17707107294095	27.434423511604184	24.93134180265419	19.457163612800674
5	22.586205587321835	40.84688146241328	19.85582411351826	16.711088836746626
6	26.525730687746314	33.300681139941226	23.82673581844368	16.34685235386878
7	77.60337962857497	8.043829874478895	8.56778367542201	5.7850068215241235
8	78.16884474514836	6.551194336236595	8.929742098295758	6.350218820319283
9	72.70074138197245	8.761418775770553	10.887101877374562	7.65073796488244
10	42.233966887132254	25.060684987331456	17.827338238755875	14.878009886780418
11	32.59296383200067	26.945146846279044	21.961004675085363	18.500884646634926
12	30.130380967568023	22.97752567247066	27.58502858965305	19.307064770308273
13	24.98702771386554	28.228960217478793	28.2608530575362	18.523159011119464
14	19.2746656946944	32.34921141156191	27.6498267408808	20.72629615286289
15	19.170887405618707	27.42379256491838	34.319986432887085	19.085333596575822
16	20.68908783946258	27.223323284557537	30.74874769979219	21.338841176187692
17	20.698453197257216	25.66892700842629	27.6574202742278	25.975199520088694
18	21.666122463443465	27.137769475514652	29.490246106415775	21.70586195462611
19	23.05067671038011	29.406717239598755	26.705191192513787	20.837414857507348
20	24.966778291606868	28.62053342040585	26.963118208533615	19.44957007945367
21	23.027389874782635	28.38184335553176	28.324385619872782	20.26638114981282
22	21.136600071379213	26.66393299466175	26.68747294803745	25.51199398592159
23	20.348138192182205	28.15834035735168	28.033047057126154	23.460474393339965
24	22.33865640020958	26.016963953497203	28.74607983840961	22.898299807883607
25	21.40895480075834	28.377793471080025	27.006148230833286	23.20710349732834
26	19.419955299400364	30.73204192642879	26.423471105340024	23.424531668830824
27	21.021937717839485	29.363940335077316	28.21959485968416	21.394527087399037
28	18.72666570481911	30.13721514758032	29.03944334338211	22.09667580421846
29	20.531142345844945	28.62382395152288	27.957111723656137	22.887921978976035
30	20.90474418651743	29.912699678287304	27.665266925353038	21.517289209842232
31	24.266148281456847	31.63187562804849	22.474580647120913	21.627395443373757
32	24.27652611036441	31.274979560739407	23.911277156373632	20.537217172522546
33	24.042898401054995	28.047221652707222	24.820223098009738	23.08965684822805
34	20.96827674885401	29.85321700040246	26.068853098035046	23.109653152708486
35	23.785224502813403	27.4928937183761	26.026329311291835	22.695552467518663
36	24.30234412374422	27.9583773125473	26.504975029931177	21.2343035337773
37	22.742379256491837	30.002809607338392	27.45036993163289	19.804441204536882
38	24.19552842132973	28.320082617642818	23.836354294016548	23.648034667010904
39	24.215271608031934	26.49231914101951	26.141244782609796	23.151164468338763
40	23.58981757801723	25.916476195538547	27.13903506440582	23.354671162038407
41	21.967585737319432	26.865667863913757	26.29387480288453	24.87287159588228
42	23.35112751314314	26.379681729705652	26.65962999243178	23.609560764719433
43	24.185656827978626	25.33177412781942	27.487325127254962	22.995243916946993
44	21.55297881657314	25.96431545562466	26.36272283856401	26.11998288923819
45	22.32270998018088	26.114920533673526	25.509462808139254	26.052906678006345
46	23.946713645326305	26.440683114259894	26.57812606784063	23.03447717257317
47	21.844823614876237	25.898504833283976	29.02552186557927	23.231149686260512
48	21.820018072609365	23.853060067379953	29.18447983030984	25.142442029700838
49	23.259751995200887	22.461418522652778	31.03553014253062	23.243299339615717
50	21.064208386804463	24.652152893262762	29.085763896798824	25.197874823133954
51	20.971567279971044	23.576402335770858	26.556104821134323	28.895925563123775
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	757.0
1	869.0
2	981.0
3	1037.5
4	1094.0
5	908.5
6	723.0
7	748.0
8	773.0
9	856.5
10	940.0
11	986.0
12	1032.0
13	1095.0
14	1158.0
15	1209.5
16	1261.0
17	1328.0
18	1395.0
19	1363.0
20	1331.0
21	1425.5
22	1520.0
23	1461.5
24	1403.0
25	1595.0
26	2472.5
27	3158.0
28	3777.0
29	4396.0
30	5048.5
31	5701.0
32	6136.0
33	6571.0
34	7997.0
35	9423.0
36	9787.0
37	10151.0
38	11823.0
39	13495.0
40	14832.5
41	16170.0
42	18952.0
43	21734.0
44	26319.0
45	30904.0
46	34531.5
47	38159.0
48	43020.0
49	47881.0
50	45039.0
51	42197.0
52	36121.5
53	30046.0
54	25676.5
55	21307.0
56	18756.0
57	16205.0
58	15479.0
59	14753.0
60	14215.0
61	13677.0
62	12435.0
63	11193.0
64	9273.0
65	7353.0
66	6354.5
67	5356.0
68	4448.0
69	3540.0
70	2872.0
71	2204.0
72	1872.0
73	1540.0
74	1296.5
75	698.5
76	344.0
77	250.0
78	156.0
79	141.0
80	126.0
81	89.5
82	53.0
83	57.5
84	62.0
85	36.0
86	10.0
87	5.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	395073.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.368080885721916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.83331347371691	23.251065008066867
2	12.18769746252814	7.889847545556372
3	5.19971856467109	5.0491473325279115
4	2.7003482509941437	3.4962036243118457
5	1.4815923326573466	2.3978150231559208
6	0.8963448922491334	1.7407778384294186
7	0.5410964039727455	1.225997651953416
8	0.3655652563576441	0.9466116629435114
9	0.3097559472871518	0.9023585000961549
>10	3.5051606102784496	29.59576409587288
>50	0.9252199713035721	18.99002121443897
>100	0.050143040402815975	3.061775083731882
>500	0.0016175174323489025	0.296596372252755
>1k	0.002426276148523354	1.1560190466621056
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	1543	0.39056073181412043	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGCT	1537	0.3890420251447201	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	1336	0.3381653517198087	Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	584	0.14782078248829963	No Hit
TCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	549	0.13896166025013099	Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	486	0.12301524022142743	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.41561939185922603	0.0
2	0.0	0.0	0.0	1.4749172937659623	0.0
3	0.0	0.0	0.0	2.0991057348895015	0.0
4	0.0	0.0	0.0	2.820238285076429	0.0
5	0.0	0.0	0.0	4.637370815013934	0.0
6	0.0	0.0	0.0	5.765010517043685	0.0
7	0.0	0.0	0.0	6.6833218164744235	0.0
8	0.0	0.0	0.0	8.154695461345119	0.0
9	0.0	0.0	0.0	8.70446727566804	0.0
10	0.0	0.0	0.0	9.83868803993186	0.0
11	2.5311777823338975E-4	0.0	0.0	11.73302149223055	0.0
12	2.5311777823338975E-4	0.0	0.0	13.08618913466625	0.0
13	2.5311777823338975E-4	0.0	0.0	13.689621917974653	0.0
14	2.5311777823338975E-4	0.0	0.0	13.96349535402318	0.0
15	2.5311777823338975E-4	0.0	0.0	14.431763243754952	0.0
16	2.5311777823338975E-4	0.0	0.0	15.332103180931119	0.0
17	2.5311777823338975E-4	0.0	0.0	16.411397387318292	0.0
18	5.062355564667795E-4	0.0	0.0	17.647877733988402	0.0
19	5.062355564667795E-4	0.0	0.0	18.38343799753463	0.0
20	5.062355564667795E-4	0.0	0.0	18.969911889701397	0.0
21	5.062355564667795E-4	0.0	0.0	19.663707719839117	0.0
22	5.062355564667795E-4	0.0	0.0	20.381043503352544	0.0
23	5.062355564667795E-4	0.0	0.0	21.117869355789942	0.0
24	5.062355564667795E-4	0.0	0.0	21.662578814548198	0.0
25	5.062355564667795E-4	0.0	0.0	22.18400143770898	0.0
26	5.062355564667795E-4	0.0	0.0	22.64467579409375	0.0
27	5.062355564667795E-4	0.0	0.0	23.08459449266338	0.0
28	5.062355564667795E-4	0.0	0.0	23.545015731269917	0.0
29	5.062355564667795E-4	0.0	0.0	24.04087345882913	0.0
30	5.062355564667795E-4	0.0	0.0	24.603048044285487	0.0
31	7.593533347001693E-4	0.0	0.0	25.09688082961883	0.0
32	7.593533347001693E-4	0.0	0.0	25.655258648401688	0.0
33	7.593533347001693E-4	0.0	0.0	26.186552864913573	0.0
34	7.593533347001693E-4	0.0	0.0	26.719112670316626	0.0
35	7.593533347001693E-4	0.0	0.0	27.24205400014681	0.0
36	7.593533347001693E-4	0.0	0.0	27.763729741085825	0.0
37	7.593533347001693E-4	0.0	0.0	28.27654635978667	0.0
38	7.593533347001693E-4	0.0	0.0	28.782275680696983	0.0
39	7.593533347001693E-4	0.0	0.0	29.328756963902872	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCA	20	7.0276594E-4	45.0	30
GTCATGT	20	7.0276594E-4	45.0	9
ACAACCG	20	7.0276594E-4	45.0	12
ATCCTCC	25	3.8861417E-5	45.0	43
CTATGTT	20	7.0276594E-4	45.0	11
CGGAATC	20	7.0276594E-4	45.0	20
CTAGACA	20	7.0276594E-4	45.0	45
AGTCCGA	35	1.2092278E-7	45.0	44
TCCATCG	20	7.0276594E-4	45.0	24
TCGCACG	25	3.8861417E-5	45.0	1
ATTAGTC	25	3.8861417E-5	45.0	31
GGTCGTT	20	7.0276594E-4	45.0	24
CGAGTTT	35	1.2092278E-7	45.0	14
AATCACG	25	3.8861417E-5	45.0	37
GTACAAG	35	1.2092278E-7	45.0	1
TTTACCG	20	7.0276594E-4	45.0	1
GCGAATA	20	7.0276594E-4	45.0	37
CCCTACC	40	6.7957444E-9	45.0	35
TCCGAAA	25	3.8861417E-5	45.0	24
GGCACGA	20	7.0276594E-4	45.0	8
>>END_MODULE