SRR2935125.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935125.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	723034
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	4208	0.5819919948439493	RNA PCR Primer, Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT	3887	0.5375957423855586	TruSeq Adapter, Index 7 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	3121	0.43165328324809066	Illumina PCR Primer Index 3 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	1070	0.14798750819463538	Illumina PCR Primer Index 3 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	978	0.13526334861154524	No Hit
ACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	906	0.12530531067695294	TruSeq Adapter, Index 7 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG	863	0.11935814913268256	TruSeq Adapter, Index 7 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCTT	764	0.10566584697261816	Illumina PCR Primer Index 3 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCC	761	0.10525092872534349	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACGG	35	1.2110831E-7	45.000004	2
CTAACGC	55	1.8189894E-12	45.000004	29
TATCACG	40	6.8084773E-9	45.0	1
CACGGAA	20	7.0315483E-4	45.0	8
ACCGAAC	20	7.0315483E-4	45.0	11
GCCGGAC	20	7.0315483E-4	45.0	13
ATTCGTC	20	7.0315483E-4	45.0	19
CGTTCCA	20	7.0315483E-4	45.0	24
CGCTACA	20	7.0315483E-4	45.0	29
CGCTAAC	25	3.8893646E-5	45.0	37
TGCGAAG	40	6.8084773E-9	45.0	1
TCGTCTC	20	7.0315483E-4	45.0	31
ACTACGG	45	3.8380676E-10	45.0	2
TATTACG	20	7.0315483E-4	45.0	21
CTACCGA	25	3.8893646E-5	45.0	40
GTCCCCG	20	7.0315483E-4	45.0	29
GACGCGA	40	6.8084773E-9	45.0	26
GCTACGA	25	3.8893646E-5	45.0	10
ATCGGAA	25	3.8893646E-5	45.0	21
GCTAACG	20	7.0315483E-4	45.0	1